Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,8110CT59.3% 10.7 / 23.9 27intergenic (‑217/‑69)ybcV/ybcWDUF1398 domain‑containing protein YbcV/uncharacterized protein YbcW
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base T (10/6);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATA  >  NC_000913/579563‑580054
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
aaTATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCAATGCGATACGAGGGCGCGTCGTTTGCATTATAGTCTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTCGCTGATGCTTTATCTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACACCCGACAGATGTATGTACGGCCAACGTGCTCAAAcat                                                                                                                                                                                                                                                   >  1:159638/1‑249 (MQ=255)
 atatTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATCATCGTTTTTATCGTTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTAAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTCTGTAAGGCCAACGTGCTCAAATCtt                                                                                                                                                                                                                                                  >  1:139390/1‑251 (MQ=255)
   atTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTc                                                                                                                                                                                                                                                 >  1:230308/1‑250 (MQ=255)
                        tGAGCCATTGGTAAAACCTTCCATGTGATACAAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCACTCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCACATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGCAGTAat                                                                                                                                                                                                                           >  1:148595/1‑251 (MQ=255)
                              aTTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGACTGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATAtttt                                                                                                                                                                                                                      >  2:89699/1‑250 (MQ=255)
                                               tgtgATACGAGGGCGCGTCGTTTGCATTATAGTTTTTATCGTTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCACATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAag                                                                                                                                                                                                       >  1:189366/1‑248 (MQ=255)
                                                       gAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCAt                                                                                                                                                                                            <  2:60584/251‑1 (MQ=255)
                                                       gAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTTCGTAACAAAGTGCGGTCCTGCGGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACATAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCAt                                                                                                                                                                                            <  1:223198/251‑1 (MQ=255)
                                                                           aTCGTTTTTATCGTTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATGAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATaa                                                                                                                                                                         <  2:137031/250‑1 (MQ=255)
                                                                               tttttATCGCTTCACTCTGGTCTGACCTCTTTGTGTTTTGTTGATGCTTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAACGAAc                                                                                                                                                                    >  2:128361/1‑251 (MQ=255)
                                                                                ttttATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGATCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAAc                                                                                                                                                                    <  1:18134/250‑1 (MQ=255)
                                                                                  ttATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACaa                                                                                                                                                                  <  1:139196/250‑1 (MQ=255)
                                                                                                  gTCTGTCCTTTTTGTGTTTTGTTGATGATTTATGTCAAAAATTAGGAAAGTTTTCACTTAATAGTATTGGTTGAGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGAAGTATGTAAGGCAAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGCCAAAATGAATAAAGAACAATCTTCTGATGTTCCCTc                                                                                                                                                 <  1:150547/251‑1 (MQ=255)
                                                                                                           ttttGTGTTGTTTTGATGATTTATGTTAAATATGAGGAATGTTGTCAATTAATAGGATTGTTTGCGTAACAAAGTGCGGTCCTGCTTGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGTCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCGGATGATCCTTCCGTGGATc                                                                                                                                         <  2:216237/249‑1 (MQ=255)
                                                                                                             ttGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCAGTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGAGGATCCCTCCGTTGATCTg                                                                                                                                       <  2:43842/250‑1 (MQ=255)
                                                                                                                                              ggAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGATGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTTGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCAttc                                                                                                     <  2:185062/251‑2 (MQ=255)
                                                                                                                                                                   aTTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGAtgttg                                                                                >  1:223159/1‑251 (MQ=255)
                                                                                                                                                                             tAACAAAGAGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAAAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGATTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATg                                                                      >  2:159129/1‑251 (MQ=255)
                                                                                                                                                                                cAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGATGTAATTGCATGtat                                                                   >  2:173282/1‑251 (MQ=255)
                                                                                                                                                                                cAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGtat                                                                   >  1:212202/1‑251 (MQ=255)
                                                                                                                                                                                   aGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTg                                                                                                                                             >  1:238114/1‑174 (MQ=255)
                                                                                                                                                                                   aGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTg                                                                                                                                             <  2:238114/174‑1 (MQ=255)
                                                                                                                                                                                        ggTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCTTGGAGCGACAAAATGAATAAAGAAAAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAAAATGCTTAATAGAACCATTTCTATGAGTTACCCTGATGTAGTAATTTCATGTATAGAACAt                                                            >  2:209659/1‑250 (MQ=255)
                                                                                                                                                                                                       cTGGAGGGAAATCCAACCGAAAGATGTCTGTAAGGCCAACGTGCTCACCTCTTCATCCAGAAAGCTTTGAAGAAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATACAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAACAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGa                                              >  2:21824/1‑249 (MQ=255)
                                                                                                                                                                                                                          aCAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGc                         >  1:135198/1‑251 (MQ=255)
                                                                                                                                                                                                                                   tgtaAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGt                >  1:3442/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cAACGAGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAAAAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGAGAAGCACg        >  2:239886/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGataata  <  1:89699/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
AATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATA  >  NC_000913/579563‑580054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑