Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	293,207	IS2 (–) +5 bp	intergenic (‑259/+9)	yagK ← / ← yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	293207 =	0 (0.000)	18 (0.680)	17/488	0.5	100%	intergenic (‑259/+13)	yagK/yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
	?	NC_000913	381260 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 293211	0 (0.000)	13 (0.490)	13/488	0.7	100%	intergenic (‑263/+9)	yagK/yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
	?	NC_000913	= 382590	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

ATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAG  >  NC_000913/381015‑381503                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aTCGCATCCGGTAGCGATAATACCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCGAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACTCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGAc                                                                                                                                                                                                                                                 <  1:265801/250‑1 (MQ=255)            tAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATCACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGa                                                                                                                                                                                                                                      >  1:234039/1‑250 (MQ=255)                                                         ccAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGCCGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAAt                                                                                                                                                                                                          >  2:256070/1‑233 (MQ=255)                                                         ccAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAAt                                                                                                                                                                                                          <  1:256070/233‑1 (MQ=255)                                                                     tACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAGCTACCGAGTGGGCAATAAAACATTTCGGACCAATCGTTGCTTCCGCTTCAAGATCCTCATTTTTAATCTCGTTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACGAGACTGGCTTCCTGAAGCTCCAGACAACTAATATCACTTAAATATGTGATAGTCTTAATACGAGTTTTTAGa                                                                                                                                                                             <  2:31428/250‑1 (MQ=255)                                                                                                                             aTAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGt                                                                                                                    >  1:107495/1‑251 (MQ=255)                                                                                                                                         ggACCAATAGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAACACCAATTTTTACGCCATTGATGATTATTGTGCCAGGAAGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGAAAACCAATATCACTTAAATAAGAGCTAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGCAACGCAGACGGAGTACCACACAGGa                                                                                                         >  2:84119/1‑250 (MQ=21)                                                                                                                                                         ccGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTc                                                                                        >  1:66363/1‑251 (MQ=255)                                                                                                                                                            cTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGCAAAGATCGCAATTGTTcagc                                                                                     >  1:11739/1‑251 (MQ=255)                                                                                                                                                            cTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCAATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTcagc                                                                                     >  2:87247/1‑251 (MQ=255)                                                                                                                                                                      cGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTg                                                                            >  1:237538/1‑250 (MQ=255)                                                                                                                                                                        cATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAc                                                                         >  1:77048/1‑251 (MQ=255)                                                                                                                                                                                                                               aGGACGAATAAACGCATAATTACTAGGCTGGCCCCATGTATATCCAGACAACCCATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTATACTAGTCCTTGGAGAACAGATGATTGATGTATTAGGGCAGGAGAAACGCAGACGACGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGCGCTTTGAACCGGGGATGACGGTCGCCCTCGTTGACAGGCAACATGGTGTTGCAGCCAGCCAGtt                  >  2:248276/1‑251 (MQ=14)                                                                                                                                                                                                                                              aTAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTa    >  1:53602/1‑250 (MQ=32)                                                                                                                                                                                                                                                aaTTACTAGACTGGCCCCCTGAATCTCCAGACAGCCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATGGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATTGTGTAGCAGCCAGCCAGTTAGTTCTCTTGCGTAAg  <  2:234039/250‑1 (MQ=9)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAG  >  NC_000913/381015‑381503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.