Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,147,156	IS2 (–) +5 bp	coding (19‑23/342 nt)	frwD →	putative PTS enzyme IIB component FrwD

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4147156 =	0 (0.000)	15 (0.570)	14/488	0.6	100%	coding (19/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4147160	1 (0.040)	36 (1.360)	36/490	0.0	97.2%	coding (23/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4146916‑4147160 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGC  <  NC_000913/4499511‑4499266                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             CGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGTCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATT                                                                                                                                                                                                                                                  <  1:252726/251‑1          GGGAGACTATCCATCAGCCGCTGGATGTACTGCTCCCGCTGGCTATCAGGCAGACCGATCAGTTACCGCTTCATAAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAACCATGGTCGATGAAAGCGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGAATACCTGGTGGCAGTAACTGGATTTGCCCCTAT                                                                                                                                                                                                                                         >  2:49227/1‑251                ATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCC                                                                                                                                                                                                                                   <  1:203447/251‑1                            CGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTA                                                                                                                                                                                                                       <  1:102352/251‑1                                 GATGTACTGATCCCGCTGAATATCAGGCCGATCCATCTGTTACAGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGCCGTTGAACGAGGTACCTGCGCAGTCGTCAGCTGATGTGGCAACGATGCGCGCAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACT                                                                                                                                                                                                                  >  2:197612/1‑251                                      ACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACC                                                                                                                                                                                                             >  2:219096/1‑251                                      ACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAAC                                                                                                                                                                                                              <  1:114650/250‑1                                             CCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTAC                                                                                                                                                                                                       >  2:181054/1‑250                                              CGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAA                                                                                                                                                                                                     <  1:112128/251‑1                                                            CAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGC                                                                                                                                                                                         >  1:218296/1‑249                                                                       GTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCC                                                                                                                                                                                <  1:219096/247‑1                                                                          ACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCG                                                                                                                                                                         <  1:99300/251‑1                                                                                    CAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACC                                                                                                                                                               <  1:242538/251‑1                                                                                          GGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGC                                                                                                                                                         >  1:56014/1‑251                                                                                                               CTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTT                                                                                                                                    >  1:163854/1‑251                                                                                                                        ACATGGGCGTTGAAAGATGTGCCTGCGCCGTCGACAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCATGTTACCGAGGGGGGTTAACATGGCATACCTGGAGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTCCACGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCCCTATGCGGATGCCATTCGTTATACTGCTC                                                                                                                           >  2:51737/1‑251                                                                                                                                                    CGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCAGACTTCAGGTTACCGTGGGAGGTTACAATGGCATACCTGGCGGCAGTAAATGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGCTATTCCCGTGGAGAGCGATAACCCAGCGCACTATGAGGATGCCGTTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCC                                                                                               >  1:157273/1‑251                                                                                                                                                               GATGGGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTC                                                                                    <  2:56014/251‑1                                                                                                                                                                  GTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGGTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTGATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATGCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAAGGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTG                                                                                  <  1:238479/250‑1                                                                                                                                                                    GGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTACCTGGATTTGCCCCTATATTTCCAGACCTCTGTTATCCCTTAACCCATTACACGCCCGCTGCCGCCGATATTCCCGTGGCGAGCGCTAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGACCGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTT                                                                               >  1:267619/1‑251                                                                                                                                                                           ATGCGCGACATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGACCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCACGGATCTTTGCTGCCGTTAACCCGTCTGGTTTGtgcct                                                                        >  1:220466/1‑246                                                                                                                                                                                 GAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACT                                                                  >  1:201603/1‑251                                                                                                                                                                                 GAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACAGCTGTTATCACTTAACCCATTACAAGCCCGCGGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACTCGGCTGGTTTGGGCATGATACT                                                                  <  2:170644/251‑1                                                                                                                                                                                             CGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACG                                                      >  1:82453/1‑251                                                                                                                                                                                             CGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCGGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACG                                                      <  1:27944/251‑1                                                                                                                                                                                                  CGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCGCGAACGCCTCTGCAAGGGTCTTTGCTGCCGTTAACCCGTCTGGTTGGTGCATGATACTGATGTAGTCACGCTTTA                                                 <  2:267016/251‑1                                                                                                                                                                                                   GGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTAT                                                >  1:156629/1‑251                                                                                                                                                                                                                    GGGAGGTTAAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATAACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCT                               >  2:27874/1‑251                                                                                                                                                                                                                            AAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTTCAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATT                       <  2:47323/251‑1                                                                                                                                                                                                                            AAAATGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTAT                        <  1:138181/250‑1                                                                                                                                                                                                                              aagGGCATACCTGGTGGCAGTAACTGGAGTTGCCCCTATATTGCCAGACAGCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCTGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGGTAACCCGTCTGGGTTGGGCATGATACTGATGTAGTCCCGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCC                     <  2:98894/248‑1                                                                                                                                                                                                                                TGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCT                             >  1:45983/1‑241                                                                                                                                                                                                                                TGGCATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATGCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGAGGTATTCACGCTTTATCGTGTTCACGAAGCTCTCT                             <  2:45983/241‑1                                                                                                                                                                                                                                    ATACCTGGTGGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATACCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACT               >  1:153177/1‑251                                                                                                                                                                                                                                             GGCAGTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACT      >  1:161817/1‑251                                                                                                                                                                                                                                                 GTAACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGC  >  1:27029/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             CGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAATACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCTGATGTGGCAACGATGCGCGAAATGGCAGAACGGGCCGGACTTCAGGTTACCGTGGGAGGTTAAAATGGCATACCTGGTGGCAGTAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4146916‑4147160 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGC  <  NC_000913/4499511‑4499266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.