Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,592	(T)7→6	intergenic (+45/+263)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,592	0	T	.	100.0%	98.9 / NA	23	intergenic (+45/+263)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/7);  total (16/7)

GGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGC  >  NC_000913/4628349‑4628837                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ggAGAACAACACGCCGTGGGGGGCGGATTGTTTACGGAGCCTCGGCAGGTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCTTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGATATAACCGCGATGAAACGGCTCAAAGGCGAGTGATAAAATAAGTTTTTT‑GATGAGtt                                                                                                                                                                                                                                               <  2:249719/251‑1 (MQ=255)           acGCCGAGGGTGGAGGATGGTTAACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGATGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGAGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCGGAGATAACCGCGATGAAACGGCCCAAAGGCGAGGAATAAAATAAGTTTTTT‑GATGAGTTGAAAACctct                                                                                                                                                                                                                                     >  2:191165/1‑251 (MQ=255)                gtgggtggCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGCGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTgg                                                                                                                                                                                                                                 >  2:21223/1‑250 (MQ=255)                        cGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTg                                                                                                                                                                                                                        >  1:101854/1‑251 (MQ=255)                                                gTTCGGTTGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGATCTGTTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGATTTGAAAACCTCTCTGGAAGAGGCTGTCGAGAGTAATGAAGGGTTGaaaa                                                                                                                                                                                                <  2:159911/251‑1 (MQ=255)                                                    ggTGGATCGCAAAGAGTTCCAGAACTTGCTTGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAATAAAcc                                                                                                                                                                                            <  2:120180/251‑1 (MQ=255)                                                    ggTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAAcc                                                                                                                                                                                            <  1:88501/251‑1 (MQ=255)                                                                                                   agaACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATAAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCtgttgt                                                                                                                                               >  1:74131/1‑249 (MQ=255)                                                                                                       cAATATCGGATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGCGCTGGTGCGGGAGATGCTGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGCGGCTGTCGGGATTAAACAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTACGAACTTGCGGATACTAAGGCTGTTGTAGTCCg                                                                                                                                         >  2:32893/1‑251 (MQ=255)                                                                                                              gaattcgggCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGaaaagg                                                                                                                                  <  1:241303/243‑4 (MQ=255)                                                                                                                       cGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCAcgc                                                                                                                         >  1:30078/1‑251 (MQ=255)                                                                                                                            tGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCGCGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCAcgccgta                                                                                                                     <  1:21223/250‑3 (MQ=255)                                                                                                                                       aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                         >  1:67974/1‑251 (MQ=255)                                                                                                                                       aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACATCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGCGATCCGCTACGAAATTGCGGCTACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                         >  2:76106/1‑251 (MQ=255)                                                                                                                                        ttatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGt                                                                                                        >  1:236371/5‑251 (MQ=255)                                                                                                                                            gACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGGGATGAAACGGCTTAAAGGCGAGGTAAAAAATAAGTTTTTG‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGCt                                                                                                     <  2:101854/250‑1 (MQ=255)                                                                                                                                                                    gagcTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCACAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGCTGCGACGCTTACcgc                                                                            >  2:98574/4‑251 (MQ=255)                                                                                                                                                                              gggAGATGCTGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATACAATAAGTTTTTT‑GATGAGTTGCAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCttctc                                                                    >  2:161325/1‑246 (MQ=255)                                                                                                                                                                                                     tAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCCTCTTATCAGGCCtggagattcacaccgtattg                                           >  2:77185/1‑232 (MQ=255)                                                                                                                                                                                                                 aacggCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAAtttcttggt                               >  2:52066/5‑244 (MQ=255)                                                                                                                                                                                                                      cTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt                           >  2:69036/1‑239 (MQ=255)                                                                                                                                                                                                                        cAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAgg                        >  1:204194/1‑251 (MQ=255)                                                                                                                                                                                                                                aGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATa                 >  2:119861/1‑250 (MQ=255)                                                                                                                                                                                                                                         aataagttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTc         >  1:61836/7‑250 (MQ=255)                                                                                                                                                                                                                                               ttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCAcgccgc  >  1:25981/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGC  >  NC_000913/4628349‑4628837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑