Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,570	(A)6→5	coding (282/915 nt)	lgoR ←	putative DNA‑binding transcriptional regulator LgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,570	0	A	.	100.0%	126.6 / NA	28	coding (282/915 nt)	lgoR	putative DNA‑binding transcriptional regulator LgoR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/11);  total (17/11)

AAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC  >  NC_000913/4595328‑4595800                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       aaGTGCTGAAGTGAAGGGGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTGCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCa                                                                                                                                                                                                                               <  1:139989/251‑1 (MQ=255)     gCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGATGGTTTATCATCGTGAAACA‑GCCTGCTCAAac                                                                                                                                                                                                                            >  1:123335/1‑250 (MQ=255)              aaTGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACtttgtttt                                                                                                                                                                                                                  >  2:102411/1‑251 (MQ=255)                     ttCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACt                                                                                                                                                                                                           >  1:71926/1‑251 (MQ=255)                       cGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCTTAGGATTGATCGAATTGCTTCATTCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                                                                                                                                                         <  1:259112/251‑1 (MQ=255)                                  cgTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                                                                                                                                                               >  1:28670/1‑250 (MQ=255)                                     aGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagt                                                                                                                                                                                            <  1:50918/250‑1 (MQ=255)                                                        cGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                                                                                                                                                                       >  1:55580/1‑220 (MQ=255)                                                        cGTAGGATTGATCGAATGGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                                                                                                                                                                       <  2:55580/220‑1 (MQ=255)                                                              aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                                                                                                                                                                  <  2:124345/235‑1 (MQ=255)                                                              aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                                                                                                                                                                  >  1:124345/1‑235 (MQ=255)                                                              aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTc                                                                                                                                                                    >  1:186780/1‑249 (MQ=255)                                                                                 gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATa                                                                                                                                               <  1:35747/251‑1 (MQ=255)                                                                                                                     ataACGGCCGAATATTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTTGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgc                                                                                                            <  1:61639/250‑1 (MQ=255)                                                                                                                       aaCGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACCCCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTa                                                                                                         <  2:186780/251‑1 (MQ=255)                                                                                                                          ggCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGACGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGt                                                                                                      >  1:10301/1‑251 (MQ=255)                                                                                                                                  tttAACAGATATTCTCTTGCTACAACACGTCCGA‑ACCCAGCGGCCCGAGCCAGTTGCAGTTTAGACAACGTTTAACCTGGGCGCAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGCAGCGGTAGAACCGGCGACACCGTCATCATGGACAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCACGACGCCGCATTCGCGTCAGTCGCTgcg                                                                                               >  2:240225/1‑248 (MQ=255)                                                                                                                                               ctctTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                                                 >  1:128293/1‑251 (MQ=255)                                                                                                                                                 ctTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTAAGAAAACGTTTCCCCTGGGCGTAACTGGAGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGATTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAAACTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                                               >  1:32798/1‑251 (MQ=255)                                                                                                                                                      cacacccGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGGTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCg                                                                          <  2:128293/246‑1 (MQ=255)                                                                                                                                                                  gaccccc‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                                              <  2:71926/247‑1 (MQ=255)                                                                                                                                                                                  aGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                                               >  2:51091/1‑251 (MQ=255)                                                                                                                                                                                     cAGTTGCAGTTCAGAAAACGTTTCACCTGGGCGTAACTCGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCACACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAATTCCGCCAgcgacct                                            >  2:197785/1‑247 (MQ=255)                                                                                                                                                                                                        gTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGa                          >  2:96263/1‑250 (MQ=255)                                                                                                                                                                                                                       aaCTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGAGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGGAGTGGGGAAGGGATATGACCCcgc          >  1:129301/1‑251 (MQ=255)                                                                                                                                                                                                                          tGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGcc       >  1:128594/1‑251 (MQ=255)                                                                                                                                                                                                                           ggCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCCACCTGCGTCAAGACGCCGCATTCGCGTAAGGGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGGAGTGGGGACGGGATATGACCCCGCGcc       >  2:72877/1‑250 (MQ=255)                                                                                                                                                                                                                                 ggTTTATCATCGTGAAGAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCTGCGCCATatc  <  1:191320/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       AAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCC‑GCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATC  >  NC_000913/4595328‑4595800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑