| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,614,321 | 2 bp→GT | intergenic (+61/+358) | yjjV → / ← yjjW | putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,614,321 | 0 | A | G | 91.0% | 133.9 / 3.5 | 68 | intergenic (+61/+359) | yjjV/yjjW | putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (38/23); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 4,614,322 | 0 | A | T | 92.4% | 140.1 / 1.2 | 68 | intergenic (+62/+358) | yjjV/yjjW | putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base T (38/23); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
GCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGT > NC_000913/4614078‑4614551 || gcagcGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTc < 1:468292/247‑1 (MQ=255) tCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGCTTTCAGCGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGACCCGGCGGACGAGGTTGCGCAAGAGTTGCTTAATAACACGTATACGTTGTTTACCGTGCCGTAGGCCGGATAAGGCGC‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCtt > 1:41612/1‑250 (MQ=37) aTTGCTGGAACCCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAt < 1:478377/250‑1 (MQ=255) tggtgtaaaCCGACGCGCCGGATTTGCCGCTCATCGTTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGACGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACTTTGGTGAACGTGCCGTAGGCCGGATAAGG‑GTCTCACGCCGCATCCGGCAGTTGGCGCACAATGCCAGATGCGCCGCTTGGCTCGTCTTATCATGc < 2:366512/244‑1 (MQ=37) cTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCt > 1:160270/1‑250 (MQ=255) gcgcCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGGGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGcc < 2:385827/251‑1 (MQ=255) cgcCGGATATGCCTCTCAACGGTTTACAGGGGCAGCCTACCCGCCCGGAGCAGGCGGCCTGTGTGTTTGCCGTGCTTTGCGATTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACTTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg < 1:420888/251‑1 (MQ=25) cGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGGGGGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAAc < 2:580712/251‑1 (MQ=37) ggATATGCCGCTCAACGGTTTACAGGGACAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACCATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaagactatgccgcacc > 2:445129/1‑236 (MQ=37) gatagcccGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCg < 1:66773/245‑1 (MQ=37) gATATGCCGCTAAACGGTTGTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGGGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCg < 1:6496/251‑1 (MQ=25) caccGGTTTTCAGGGGCATCCTAACCGCCCGTGGCAGGCTGCTCTTGTGTTTGCCGTGCTTTGCGTGTTGCGCGGGGAACCGGCGGATGAGATTGTGTAAGTGTTGCTTAAGATCACGTATACGTTGTTTACCGTGCCGTAGGCCGGATAAGGCGT‑TCTCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGACCCGTAGGCCGGATa < 2:630292/248‑1 (MQ=18) cAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa < 1:222535/249‑1 (MQ=255) aaCGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATaa < 1:341533/251‑1 (MQ=255) aCGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATa > 1:586917/1‑249 (MQ=255) gTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATAAGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCg > 2:440193/1‑251 (MQ=255) ttttCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTTTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGt > 1:74620/1‑251 (MQ=255) ttCAGGGGCCGCCTACCCGCCCGGACCAGGCTGCACGTGCGTTTGCCGTGCTTTGCGAGCTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATACCACGTATACGTTGTTTAACGTGCCGTAGGCCGGCTAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGCTGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc > 2:95600/1‑251 (MQ=21) cAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAc > 1:397691/1‑251 (MQ=255) aGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg > 2:523080/1‑251 (MQ=255) ggggGAGCCTAACCGCCCGGATGAGGGCGCCCGGGTGTGTGCCGGGCGTTGGGAGGTGCGCCGGGAACCGGAGGATGAGAGTGCGCAAGCGTTGCTTAATAGCTCGGATACGTTTTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTTTCGCGTCTTAGCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAc < 2:381076/249‑1 (MQ=17) ggggCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc > 2:564291/1‑251 (MQ=255) gCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGTGACGCTTGTCGCGTCTTATCATGCCTACAAATCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccgc > 2:335265/1‑251 (MQ=25) cAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGCTAAGGCGTTCAcgccgc > 2:136247/1‑250 (MQ=255) ccTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTACCGTGCCGTAGACCGGCTAAGGCGT‑TCACGCAGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCCTGCCTACAAGTCTGTGCCGAACCGTACGCCGGCTAAGGCGTTCACGCCGCCTcc > 1:623797/1‑251 (MQ=21) cTAACCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:239582/1‑251 (MQ=255) aaCCGCCCGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:170531/250‑1 (MQ=255) ccGCCCGGAGCAGGCTGCTCGTGTGTTGGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCAg < 1:125193/251‑1 (MQ=21) ccGCCCGGAGCAGGCTGCCCGGGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGGAGGCCGGATCAGGCGTTCACGCCGCATCCGGccg > 2:126126/1‑249 (MQ=21) ccGGAGCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtcgg > 1:15330/1‑248 (MQ=255) gCAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATGGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGcat < 1:335265/251‑2 (MQ=18) cAGGCTGCCGGTGTGGTGGCCGGGCTTTGCGAGGTGCCCGGGGAACCGGCGGAGGGTATGGCGCACGCGTGGCTTAATAGCCCGTGTACGTTGTTGAACGGGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTTCCATCCCTACAATTCTGTGCCGAACCGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAGtctgcgcata < 2:33200/251‑10 (MQ=2) cAGGCTGCCCGTGTGTTTGCCGTGTTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑GCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGcata < 1:278243/251‑3 (MQ=18) cAGGCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGCTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGcat > 1:216096/1‑249 (MQ=18) ggCTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt > 1:344176/1‑251 (MQ=255) cTGCCCGTGTGTTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGc > 1:604882/1‑251 (MQ=18) gtgtTTGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCg > 1:427869/1‑251 (MQ=18) gCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCtt > 1:164906/1‑251 (MQ=18) gTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGCTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGAAGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCGTCGGCGCATAATGCCTGATGCGACGCTTGTc > 1:294137/1‑251 (MQ=17) tGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCCTAATGCCTGATGCGACGCTTGTcg > 1:249541/1‑251 (MQ=17) cTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCTACGCTTGTcgcg > 2:231111/1‑251 (MQ=17) cTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcgcg > 1:470984/1‑251 (MQ=255) gCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCtt < 1:418525/251‑1 (MQ=255) gCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCtt > 1:398735/1‑251 (MQ=255) gAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACTCTTGTCGCGTCTTAt < 1:176056/251‑1 (MQ=17) gcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCt > 1:119354/1‑251 (MQ=255) ccGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTCGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCtc > 2:428371/1‑250 (MQ=17) cGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCCTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTAc > 2:384056/1‑251 (MQ=14) gggAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTGTCATGCCTACAAGTCTGTGCCGAACCGAAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATACCTGATGCGAAGCTTGTCGCGTCTTAACATGCCTAcc > 2:604988/1‑250 (MQ=14) gaaaCCGGCGGAGGGGATTGCGCAAGCGTTGTTTAATAACACGTACACGTTGTTTAAGGGGCCGTGGCCCGGATATGGGGT‑TCCCGCCGCATCCGGCGGTTGGCGCCCAATGCCGGAGGCGACGCTGGTCGGGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTGATCATGCCTACag < 2:613116/249‑2 (MQ=2) aaaCCGGCTGATGGGATTGCGCAAGCGTTGCTTATTAACACGTATACGTTGTTTAGCGGGCCGTAGCCCGGATAGGGTGT‑GCTCGCCGCATCCGGCAGTTGGCGCCCAATGCCGGGTTCGACGCTTGTCGCGTTTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACagg < 2:227158/250‑3 (MQ=11) aaCCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTATTATCATGCCTACaggt > 1:325204/1‑248 (MQ=14) gcggATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTGGTTTAACGGGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCACCCGGCAGTGGGCGCACAATGCCTGATGCGACGCTTGTCGCGTTTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTtgtg < 1:231111/251‑1 (MQ=11) gATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCg > 1:204733/1‑251 (MQ=11) aTGAGATAGCGCACGCGTTCCTTAATAACACGTCTACGTTCTTTAACGTGCCGTACGCCGCCTAAGGCGT‑TCACGCCGCATCCAACAATCGGCGCACAAAGCCTGATGCGACGATTGTCGCGGCTTATCCTGACTACAAGTCTATGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCACACAATGCCTGATGCGCCGCATATCACGTCTTaccacacctgcaggattgggccg > 2:359723/1‑228 (MQ=2) gagaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAAc > 1:473794/1‑251 (MQ=11) agaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTCACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAAcc > 1:506679/1‑251 (MQ=11) tGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGt > 1:171059/1‑249 (MQ=11) cgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc < 1:40747/251‑1 (MQ=11) gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGcc < 1:334821/251‑1 (MQ=11) cAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCg > 1:276819/1‑251 (MQ=11) gTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAGGCGACGCATAATGCCTGATCCGACGCTTGTCGCGTCTTATCCTGCCTACAAGTTTGTCCCGaaacggcggccggttac > 2:539640/1‑236 (MQ=11) ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATa > 1:257836/1‑249 (MQ=11) atccGTTGTTTACCGGGCCGTAGGCGGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:169305/248‑1 (MQ=255) ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:500179/1‑251 (MQ=255) tcgtggtgcagcgggCCGGAGGCCGGGTAAGGCGT‑CCGCGCCGCATCCGGCAGTTGGCTCCAAAGGCCTGATGCGCCGCTTAACGCGTATTATAATGCCTACAGGTTGGGGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCAGTTGGCGCACAATGCCTGATGCGACGCTTGGCGCGTCTTATCATGCCTACAGGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATcc < 2:148976/236‑1 (MQ=14) ggCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTAGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGACTGTGCCGAACCGTAGGCCGGGTACGGAGTTCACGACGCATCCGGCCGTAGGCGCagaaggcccgagtcggccc > 2:426681/1‑233 (MQ=14) caATGCCTGATGCGACGCTTACCTCGTCTTATCATGCCTACAGGTTTGTGCCGAACCTAAGGCCGGATAAGGCTTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTtgt < 1:54304/251‑1 (MQ=255) || GCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGT‑TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGT > NC_000913/4614078‑4614551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |