| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,497,385 | 4 bp→CAA | coding (530‑533/1656 nt) | opgD → | glucans biosynthesis protein D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,497,385 | 0 | T | C | 95.0% | 52.5 / ‑4.2 | 20 | F177S (TTT→TCT) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (11/8); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| * | NC_000913 | 1,497,386 | 0 | T | . | 95.0% | 76.9 / ‑3.0 | 20 | coding (531/1656 nt) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (11/8); minor base C (0/1); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
| * | NC_000913 | 1,497,387 | 0 | C | A | 100.0% | 55.2 / NA | 20 | L178N (CTC→AAC) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (11/9); total (11/9) | |||||||||||
| * | NC_000913 | 1,497,388 | 0 | T | A | 100.0% | 51.0 / NA | 20 | L178N (CTC→AAC) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/9); total (11/9) | |||||||||||
CATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCC > NC_000913/1497174‑1497600 ||| cATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGCTGATACatat > 2:67872/1‑251 (MQ=255) atatGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATc < 1:439456/251‑1 (MQ=255) gccgcGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAACTTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCcgcg > 2:442329/1‑251 (MQ=255) cgcgTTCGTATGTTTTCTGTAGAGCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGc < 2:314155/251‑1 (MQ=255) acTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGCCACt > 2:48490/2‑251 (MQ=255) gTAGATCCATCAACCCATCTGGCGCGTGAAATTCCCTTTCGCCTGGAGGTGTTCAAATACAACGAGGCAGGTGTTGATACAAAACAATGAGAAGGGCAAATCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCTCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATAGCAATACGGTTTGTCGGCCCGCGGCCTGGCGAGCGTCACt < 2:34217/248‑1 (MQ=255) catcacaTCTGTCGTGTGAAATTCACTTGCGCCCGGAGTTGTTCAAATACAACGATGCATGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTGTAAAGCCCCCGAACTGGCTCGCCGTGATGTAGGAGC‑GTCAACGGCGCGAGTTATTTCCGCGCCTTTGATGATACATATGAATTCGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTa < 1:261007/248‑1 (MQ=255) cacaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTaa > 2:94255/1‑249 (MQ=255) acaTCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAaaga < 1:94255/251‑1 (MQ=255) ggCGCGTGAAATTCACTTTCGCCCTGAGTTGTTCAAAAACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGGAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCGGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACCCCGACAGTAaagaag < 1:427515/247‑1 (MQ=255) gcgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTccc < 1:12171/251‑1 (MQ=255) tCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGCTCTCGGCTTTGCCGGTTTTCGCGTGTTTACATCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGCCAGTAAAGAAGAGTTCCCCGCCTTTACCGCCTTCTGGTTTGATACgg > 1:412708/1‑251 (MQ=255) aCGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACGGGCGCGCCGTGATGTATTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTGACCGCCTTCTGGTTTGATACGGTAAAACCgg < 2:574339/251‑1 (MQ=255) gATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCgggg > 2:415200/1‑251 (MQ=255) cAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGCTTTTCGCGTGTTTAAAGACCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATCCGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGACTTCCCCGCCTTTACCGCCTTCTGGTTTGATACGGTCACACCGGGGGCAACTACCTTTACCGTTTAt > 2:292013/1‑251 (MQ=255) aaaGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGAt > 1:616600/1‑250 (MQ=255) aaGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCATTACCGTTTATGCGTTGCTCGAt > 2:165463/1‑249 (MQ=255) gtcTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCAtt < 1:313324/249‑1 (MQ=255) cGGTTTTCGCGTGTTTAAAGCCCCCGCACTGGTGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGCCAGTACAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGCTACGGTAACACCGGGGGCAACTACCTTTACCGTTTCTGCGTTGCTCGATAGCGCCAGCCTTACTGGTGc > 2:435889/1‑251 (MQ=255) aGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATcc > 1:545977/1‑250 (MQ=255) ||| CATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCC > NC_000913/1497174‑1497600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |