| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 364,170 | 2 bp→AA | coding (2135‑2136/3075 nt) | lacZ ← | beta‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 364,170 | 0 | G | A | 97.1% | 208.8 / ‑5.9 | 70 | A712V (GCC→GTT) | lacZ | beta‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (29/39); minor base C (2/0); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 364,171 | 0 | G | A | 98.6% | 208.6 / ‑6.3 | 70 | A712V (GCC→GTT) | lacZ | beta‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (31/38); minor base C (0/1); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
CCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCGGCTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCC > NC_000913/363935‑364406 || ccAGCGGTGCACGGGTGAACTGATCGCTCAGCGGCGTCAGCAGTGGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCACCTTCTGACCATGc < 1:456791/250‑1 (MQ=255) gCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAAGCCAATTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCTGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CTACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCACGCGG‑TCGCGtt < 2:456875/251‑1 (MQ=255) cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGTCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTc < 1:573214/251‑1 (MQ=255) aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTGCTGACCATGCGG‑GCGCGTTCGGTTGCACt < 1:501768/251‑1 (MQ=255) gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCGCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTAc < 1:56677/250‑1 (MQ=255) gcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACt < 1:481828/251‑1 (MQ=255) aGCGGCGACTGCAGTTGGTTTTTATCGCCAATCCACATCTGTTAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATGTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACGTCTGACCATGCGT‑TCTCGTTCGGTTGCACTACGCGTACtgt < 2:207214/251‑1 (MQ=255) gcgccgTCAGCAGTTGTTGTGTTTCTCCATTCCACAGCTGTGAAAGTAATCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGAGGCAAAAATCCAGTTCGCTGGTGGGCAGATGCGGGATGGCGTGTGACGCGGCGGGGTGCGTCGCACT‑GAGGTGTTCCGGCTGTCG‑CCACTGCTGCCAGGCGCTG‑ATGTTCGTAACTGCTGACCATGCGT‑TCGCGTTCGTTTGCACTACGCTTACtgtg < 2:424673/247‑1 (MQ=255) cggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGTCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGGGGTCAGATGCGTGATGGCGTGGGACGCTGCGGGGAGCGTCACACT‑GAGTTTTGCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGGCCATGCGG‑GCGCGGTCTGTTTCACTACGCGTGCTGTGa < 2:301661/251‑1 (MQ=255) ggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGTTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCg < 1:418413/219‑1 (MQ=255) gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCCTCTCGATGCAAAAAACCATTTCGCTGGTGGTTCGATGCGGGGTGGCGTGGGGCGCGGCGGGGGGGGGCACCCTTGGGGTTTTTC‑CCCGACG‑CCCCCGCTGCCCGGCGCGG‑GTGTGCCCCACTTCTGTACATACGG‑TCGCGTTCGGGTGCCCCACCCCTCCTGTGGGCCCGAGTTGcccc > 1:429841/1‑250 (MQ=255) aTCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCCAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGctct < 1:109969/251‑1 (MQ=255) cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTcc < 1:94974/251‑1 (MQ=255) ccACATCTGTGAAAGAAAGCCTGACTTGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GCGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTTCCCGGCGCTCTCCGGCTGCg < 1:514449/251‑1 (MQ=255) gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCCTTTTCCTGGTGGTCAGATGAGGGATGGCGTGGGACGCGGCGGGGGGCGGCACACT‑TGGGGTTTCCCCCCGACGGCCCCT‑CTGCCCGGCGCCGCATG‑GGCCAAATTCTGACCATGCGGGTGGGGTT‑GGGTGCACCACCCGTACTGTGAGGCAGAGTTGGCCGGCGCGCTCCGGcgtcgggaggtt > 1:82226/1‑239 (MQ=255) gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGt < 2:502737/248‑1 (MQ=255) gggaaagaaaGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTc < 2:337221/248‑1 (MQ=255) ggaaagaaaGCCTGGCTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAg < 1:597727/250‑1 (MQ=255) gaaagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGt < 2:388077/251‑1 (MQ=255) aagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCATGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTtc < 1:207102/251‑1 (MQ=255) ccTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGtt < 1:111368/211‑1 (MQ=255) cGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCTGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGttt < 2:478162/251‑1 (MQ=255) gTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGCTGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GCGGTTTTTCGCCAGACG‑CCACTGGTGCCAGGCGCTG‑ATGTGCCCCACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGCACTGTGAGCCAGAGTTGGCCGGCGCTCTCCGGCTGCGGTAGTTAAGGCAGTTCAATCAACTGttttc > 2:620487/1‑249 (MQ=255) gTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCAGTGAGCGTCACACT‑GAGGTTTTTCGCCAGACG‑CCACTGCTGTCCCGCGCTG‑ATGTGCCCAACTTTTGACCCTTCGG‑TCGCGTTCGGTTGCCCTACTCGTACTGTGAGCCAGAGTTGCCCTGCGCTCTCCGGCTGCGGTCGTTCAGGAAGTTCAATCACATGTTTAc > 2:356074/1‑251 (MQ=255) tAAATTGCCAACGCTTATTCCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACCCT‑GGGGTGTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTTCCCAACTTCTTACCATGCGG‑TCGCGGTCGGTTGCACTACGCGTACTGTGCGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTGGTTCAGGCAGTTCAATCAACTGTTTAccc > 2:131979/1‑250 (MQ=255) aaaTTGCCAACACTTATTACCCAGCTCGATGCAAAAATCCATTTCGGTGGGGGTCAGATGCGGGATGGCGTGGGACGCGGAGGGGAGCGTCACAAT‑GCGGTTTTCCGCCAGGCG‑CCACTTCTACCAGGCGCTG‑AGGTGCCCAACTTCTGACCATGCGG‑TAGCGTTCGGTTGCGCTACGCCTACTGTTAGCCAGAGGTGCCCGGCGCTCTCCGGCTGCGGTAGTTCCGGCAGTTCAATAAACTGTTTACCtt > 1:385435/1‑251 (MQ=255) aaTTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTtg > 1:523402/1‑251 (MQ=255) aTTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGGTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTTAATCAACTATTTACCTtgt > 1:270397/1‑251 (MQ=255) aaCGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCg > 1:177678/1‑251 (MQ=255) cGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCCGGCGCTG‑ATGTGCCCAAATTCTGACCATGCGG‑TCGCGGTCGGTTGCAATACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGGTCAATCAACTGTTTACCTTGTGGCGCGAc > 1:39474/1‑251 (MQ=255) attaCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATcc > 1:99380/1‑250 (MQ=255) taCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCag < 1:322134/250‑1 (MQ=255) cccAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAgg > 1:193090/1‑251 (MQ=255) ccAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGCTGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCCGACG‑CCCCTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTCCTGTGAGCCCGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCCGTTCAATCAACTGCTTTCCTTGTTGAGCGACCTCCAGAGGc > 2:426038/1‑251 (MQ=255) ccAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGGCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGc > 2:157545/1‑251 (MQ=255) aGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCAc > 1:480426/1‑251 (MQ=255) tCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCCCTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGGCGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTc > 2:174306/1‑251 (MQ=255) ccGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTc < 1:321481/250‑1 (MQ=255) aaaaaTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCtt > 1:86064/1‑251 (MQ=255) aTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGCGGAACGTCACACT‑GAGGTTTTCCGCCAGACG‑ACACTGATGCCCGGAGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGTCAGAGTTGCCCGGCGCTCTCCCGCTGCGGTAGTTCCGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGATATCAAGAGGCACTTCCCCGCTTGcc > 1:288502/1‑250 (MQ=255) cATTGCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCg < 1:228387/251‑1 (MQ=255) tggtggTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGGTCAATCAACTGATTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTAcc > 1:475904/1‑250 (MQ=255) ggtggtCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCTGCTTACCAt < 2:587249/251‑1 (MQ=255) ggCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGt < 1:295639/251‑1 (MQ=255) ggCGTGGGACACTGCGGGGAGCGTCCCATT‑GAGGTTTTCCGACAGAGG‑CCACGGCTGCCAGGCGCTG‑ATGGGCCCAACTTCTGACCATGCGG‑GCGTGTTCGGTTGCCCTACGGGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGTAGCGACATCCAGAGGCAGTTCACCGCTGGCCAGCGGCTTACCATCCAGCGCCACCATCCAGt < 2:433237/251‑1 (MQ=255) gCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTg < 1:262019/251‑1 (MQ=255) cGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGc > 1:170409/1‑251 (MQ=255) ggACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTGCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGa < 1:284490/251‑1 (MQ=255) ggggcggGGAGCTGCACACT‑GGTGTTATCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTGTTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGGACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAGCGCCTTCATCCAGTGCAGGAGCtt < 2:117679/249‑2 (MQ=255) gcggcgGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTc < 2:291315/251‑1 (MQ=255) gcggGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGtt < 2:439392/251‑1 (MQ=255) tgacgcgCGTCACACT‑GAGGTTTTCAGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGGTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGAGCAGGAGCTCGtt > 2:380543/7‑250 (MQ=255) cccacT‑GAGGTTTTCCGCCAGACT‑CCTCTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGGTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCGTACCATCCAGCGCCGCCATCCAGTGCATGAGCTCGTTATCGCTATGAc < 2:611024/249‑1 (MQ=255) acT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCAGCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGa < 1:566321/251‑1 (MQ=255) cT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGa > 1:432631/1‑250 (MQ=255) gAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAAc > 1:467248/1‑250 (MQ=255) aGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAAc > 1:366723/1‑249 (MQ=255) aGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTGGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAAGCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAg < 1:23539/251‑1 (MQ=255) ggTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAgg > 1:625175/1‑251 (MQ=255) tttCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTa < 1:585338/250‑1 (MQ=255) ccGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCg < 1:57282/251‑1 (MQ=255) cGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACCGGAATTCGc > 2:449739/1‑251 (MQ=255) gCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGGGCCCAACTTCTGACCACGCGG‑TCGCGTTCGGTTGCACTACGCGAACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGGGAGACATCCAGAGGCACTTCAACGCTTCCCAGCGGCTTACCATCCAGCGCCACCATCCAGTACAGGAGCTCGCTATCGCTATGACGTAACGGGTAGTCGCt > 2:260039/1‑251 (MQ=255) gCCAGACG‑CCACTGCTGCAAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCt > 1:163611/1‑251 (MQ=255) cAGACG‑CCACTGCTGCCATGCGCTG‑ATGTGCCCAACTGCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTgg < 2:425319/251‑1 (MQ=255) cgcggcCAGGCGCTT‑ATGTGCCCAACTTCTGACCAGGCGG‑TCGTGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGCTATCGCTATGACGGAACAGGTACTCGCTGGTCACTTCGAt < 2:497204/247‑1 (MQ=255) cAGGCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTg > 1:215811/1‑251 (MQ=255) ggCGCTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTACCTTCGATGGTTTGcc > 2:88204/1‑251 (MQ=255) ggCGCTG‑ATGTGCCCAACTGTTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGcc < 1:380543/251‑1 (MQ=255) gcgcTG‑ATGTGCCCAACTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGAATTCGCTGGTCACTTCGATGGTTTGccc > 1:412896/1‑251 (MQ=255) || CCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACT‑GAGGTTTTCCGCCAGACG‑CCACTGCTGCCAGGCGCTG‑ATGTGCCCGGCTTCTGACCATGCGG‑TCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCC > NC_000913/363935‑364406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |