Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,857	T→C	C388R (TGC→CGC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,857	0	T	C	100.0%	232.7 / NA	71	C388R (TGC→CGC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/37);  total (34/37)

ATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAC  >  NC_000913/4637613‑4638100                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aTTGGTAGAAACGTTACTACGCCAGGCAAGACGGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGGTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCGGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAtaa                                                                                                                                                                                                                                               <  2:85916/251‑3 (MQ=255)       aGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTa                                                                                                                                                                                                                                          <  2:447361/250‑1 (MQ=255)         aaaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGAG‑CAGCGCGCACCGGGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGGTACTGGCGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgc                                                                                                                                                                                                                                       >  1:280968/1‑251 (MQ=255)          aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACGGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACCGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgcg                                                                                                                                                                                                                                      <  1:627929/251‑1 (MQ=255)            cGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGcc                                                                                                                                                                                                                                    <  1:181235/251‑1 (MQ=255)                     gCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGAt                                                                                                                                                                                                                           <  1:46855/251‑1 (MQ=255)                                   gcgaATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGGATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGGGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTcgc                                                                                                                                                                                                             >  2:17545/3‑249 (MQ=255)                                       aTCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCTCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTcgccctt                                                                                                                                                                                                         <  1:396591/251‑7 (MQ=255)                                       aTCGTCAGAAAGTCGATCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCAGCG‑AAGCGGGAACCGTGCAGGTGACCGAAAAAAACATCACTCTACATTTTATGCCCACTGCGGTTAATGTTGCTGCTGAACCGGAGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGCCGAATAACGCTCAGAGCCGTAGTGGCTCAGGCACACGTccccctt                                                                                                                                                                                                         >  2:379221/1‑245 (MQ=255)                                                aaGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCAGCGCAAG‑GCGCACCGTGCAGTTGGCAGCACAAAACATACCTCTACAAGTTATGCCCACTGAGGTTAATGTTGCTGCTTCACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACCCTAAGCCCCGGAGTGGGTCAGGAACAGGTGGCCCTTAAGGTGCTg                                                                                                                                                                                                >  2:81394/1‑251 (MQ=255)                                                    cGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTTCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGAt                                                                                                                                                                                             <  1:313093/250‑1 (MQ=255)                                                                 gTTGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACGCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTa                                                                                                                                                                                <  2:194143/250‑1 (MQ=255)                                                                   tGATGTGGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTAtt                                                                                                                                                                              <  2:248124/250‑1 (MQ=255)                                                                       gtgGCGGCATTCTTTAGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGGGGATCAGGAACACGTCGCCCTTAAGGGGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                          >  1:483271/1‑250 (MQ=255)                                                                       gtgGCGGCATTATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGATACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGAACAGGAACACGTCGCCCTTAAGGAGCTGGATACCGGTAGGGGTATTCCTgg                                                                                                                                                                         >  1:623454/1‑250 (MQ=255)                                                                       gtgGCGGCAATATTTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGGAGTAAAAAACATCACTCTCCATGTTATGCCCCCTGAGGTTACTGTTGCTGCTGCACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGAATAACGCTAAGCGCCGAAGGGGGTCAGGAACACGTCGCCCATAAGGTGCTGGATACCGGTAGTGGGATTcatg                                                                                                                                                                          >  1:363722/1‑247 (MQ=255)                                                                                 tattTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCTCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCt                                                                                                                                                                <  1:259783/250‑1 (MQ=255)                                                                                 tattTCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCt                                                                                                                                                                <  1:536973/250‑1 (MQ=255)                                                                                     tcgccgcGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCa                                                                                                                                                            <  1:248915/249‑1 (MQ=255)                                                                                      cgccgcGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCAc                                                                                                                                                           <  1:408735/250‑1 (MQ=255)                                                                                        ccgcGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAACACCTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTACCCCCGAGAGCGGTCGCATAACGCTACGCGCCGAAGTGGCTCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACga                                                                                                                                                         >  2:57617/1‑249 (MQ=255)                                                                                        ccgcGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTa                                                                                                                                                        <  1:348878/251‑1 (MQ=255)                                                                                        ccgcGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGGAGTGGCATTCCTGACTCCGCGCTGTCACGt                                                                                                                                                         >  2:244836/1‑250 (MQ=255)                                                                                         cgcgTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAt                                                                                                                                                       <  1:180501/251‑1 (MQ=255)                                                                                              cAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAttttt                                                                                                                                                   <  1:221011/250‑1 (MQ=255)                                                                                                 cG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGACGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCGGACTACGCGCTGTCACGTATTTTTGAAc                                                                                                                                               <  2:222434/251‑1 (MQ=255)                                                                                                 cG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCAGCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAAc                                                                                                                                               <  1:590521/251‑1 (MQ=255)                                                                                                       cgcgcACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCttt                                                                                                                                          >  1:265195/1‑251 (MQ=255)                                                                                                          gcACCGTGCAGTTGGCAGAAAACCACCTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGACGGGGATCAGGACCACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGACAGCTTTTAc                                                                                                                                       >  2:397785/1‑251 (MQ=255)                                                                                                          gcACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTAc                                                                                                                                       >  1:391613/1‑251 (MQ=255)                                                                                                            aCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGAACCGACGTTACTGGAGCAGGCGCTGGGGAATTTCCTGGATAACGCCATCGATTTTAACCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAAGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                     >  1:289193/1‑251 (MQ=255)                                                                                                             ccGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActct                                                                                                                                    <  2:388765/251‑1 (MQ=255)                                                                                                                   aGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGCACGCTTTTACTCTTTGccg                                                                                                                              <  1:473832/251‑2 (MQ=255)                                                                                                                         cAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCa                                                                                                                        <  2:375002/251‑1 (MQ=255)                                                                                                                                 aacaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGc                                                                                                                 >  1:329851/4‑250 (MQ=255)                                                                                                                                  acaTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCa                                                                                                                >  1:620401/3‑250 (MQ=255)                                                                                                                                   caTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaa                                                                                                               >  1:218688/2‑250 (MQ=255)                                                                                                                                    aTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaaa                                                                                                             <  1:130333/251‑1 (MQ=255)                                                                                                                                         tctacgtGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTACGCGCCGAAGTGGATAAGGCACACGTCGCCCTTAAGGTGCTGGATACCGGTAGGGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCACAAAagc                                                                                                         >  2:593237/7‑250 (MQ=255)                                                                                                                                               tGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTc                                                                                                  >  2:176506/1‑251 (MQ=255)                                                                                                                                                gTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCt                                                                                                 <  1:54816/251‑1 (MQ=255)                                                                                                                                                         actgAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGc                                                                                        <  1:503000/248‑1 (MQ=255)                                                                                                                                                         actgAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCCGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATACCGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGTCGCTGGAGACCGGTCGTGGTATTCCTGCATACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGAAGCGGTCTGGGGTTGGc                                                                                        <  1:572714/248‑1 (MQ=255)                                                                                                                                                             aGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc                                                                                    >  1:223231/1‑251 (MQ=255)                                                                                                                                                                   atgTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGACCACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGt                                                                              >  2:311869/3‑251 (MQ=255)                                                                                                                                                                    tgTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGCTTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTg                                                                             >  2:511761/2‑251 (MQ=255)                                                                                                                                                                     gTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGGTGGCGTTCGTCAGTgg                                                                            >  2:241288/1‑250 (MQ=255)                                                                                                                                                                       tgctgcTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGGTGGCGTTCGTCAGTGAgg                                                                          >  1:458514/1‑251 (MQ=255)                                                                                                                                                                       tgctgcTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCGTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGTCAGTGAg                                                                           <  2:620401/250‑1 (MQ=255)                                                                                                                                                                          tgctgAACCGGCGTTACTAGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGCTCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCCCGTATTTTTGACCGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAgggcg                                                                       >  1:105271/1‑248 (MQ=255)                                                                                                                                                                           gctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTTGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGTTCg                                                                       <  2:492959/250‑1 (MQ=255)                                                                                                                                                                              gAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGTCAGTGAGGTCGccc                                                                    <  2:623454/250‑1 (MQ=255)                                                                                                                                                                                   ggCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgtttgt                                                              >  1:246951/1‑251 (MQ=255)                                                                                                                                                                                   ggCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgtttgt                                                              >  2:451284/1‑251 (MQ=255)                                                                                                                                                                                            ggAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGc                                                      <  2:483271/250‑1 (MQ=255)                                                                                                                                                                                                        ggggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActct                                                                                                                                    <  1:159407/156‑1 (MQ=255)                                                                                                                                                                                                        ggggaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActct                                                                                                                                    >  2:159407/5‑160 (MQ=255)                                                                                                                                                                                                           gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgcc                                      >  2:262077/2‑250 (MQ=255)                                                                                                                                                                                                               ttACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                  >  1:200301/1‑251 (MQ=255)                                                                                                                                                                                                                tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACCCGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGCGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTg                                 >  1:50753/1‑251 (MQ=255)                                                                                                                                                                                                                           gCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGGCGCCCGTTTGTGTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTgg                      <  2:50753/251‑1 (MQ=255)                                                                                                                                                                                                                                cGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGc                 >  1:516058/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGGGGCGTGCTGGcc            >  2:474262/1‑251 (MQ=255)                                                                                                                                                                                                                                       aCCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTc          <  1:17545/251‑1 (MQ=255)                                                                                                                                                                                                                                        cccccGAGAGCGGTCGCATAACGCTAAGCGCCGCAGAGGATCAGGCACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGATGTCGCCCGTGTGTTTAACGGCGAAGTCACGCTGCACAACGTGCAGGAAGGTGGCGTGCTGGCCTCg         >  2:123140/1‑251 (MQ=255)                                                                                                                                                                                                                                        cccccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCg         <  1:311869/251‑1 (MQ=255)                                                                                                                                                                                                                                        cccccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCg         <  2:65720/251‑1 (MQ=255)                                                                                                                                                                                                                                          cccGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCt       <  1:244836/251‑1 (MQ=255)                                                                                                                                                                                                                                             gagaGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCg    <  2:223231/251‑1 (MQ=255)                                                                                                                                                                                                                                              agagCGGTCGCATAACGCTAAGCGCCGAAGTGGATCCGGAACACGTCGCCCTTAAGGTGCTGGATACAGGTAGTGGTATTCATGACTACGCGCTGTCACGTATTTTTGAACGATTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCACGTTTGTTTAAAGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCtt      >  1:358801/1‑248 (MQ=255)                                                                                                                                                                                                                                               gagCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAc  <  1:81394/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCG‑AAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAC  >  NC_000913/4637613‑4638100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑