Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,577	T→C	T294T (ACT→ACC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,577	0	T	C	100.0%	231.3 / NA	71	T294T (ACT→ACC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/37);  total (34/37)

GCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG  >  NC_000913/4637344‑4637823                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gccgATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCa                                                                                                                                                                                                                                       <  1:171631/248‑1 (MQ=255)   cgATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACGCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATGCTGACGCaaa                                                                                                                                                                                                                                     <  2:505262/250‑1 (MQ=255)        ccGTCCCTGACAATAAGTCCGTTCCTCTTCCTTAACTATGCAGTAGCAATGTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATACGCATGAAGCTGGAAGGTAAAAACTATAGTGAGCAGTATGTTTATGCGTTAACCCTTGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACCCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTGCCGACAACATTCTGACGCAAAATgct                                                                                                                                                                                                                                <  1:436499/251‑2 (MQ=255)        ccGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATgcg                                                                                                                                                                                                                                <  1:436511/251‑1 (MQ=255)         cGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATgcgc                                                                                                                                                                                                                               <  1:175883/251‑1 (MQ=255)          gTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGCGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGcgcg                                                                                                                                                                                                                              >  2:180501/1‑251 (MQ=255)                 aCAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACAGGAAGTGGTGGCTCGTTTTACCGACAACATTCTTACGCAAAATGCGCGTATGCAg                                                                                                                                                                                                                       <  1:491267/251‑1 (MQ=255)                   aaTAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTCTGCAGGc                                                                                                                                                                                                                     >  2:46855/1‑251 (MQ=255)                      aaGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCTCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGAGTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAtt                                                                                                                                                                                                                  <  1:607748/251‑1 (MQ=255)                      aaGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAtt                                                                                                                                                                                                                  <  2:389666/251‑1 (MQ=255)                       aGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTg                                                                                                                                                                                                                 <  1:130081/251‑1 (MQ=255)                         cccGGTCCTCTGCCTGAACTCGGCAGTAGCGAGTGGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACGGGCGGCGATTCGTGGCGCTGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACAGTCTGACGCAAAATGCGCGTAGGCAGGCATGGGt                                                                                                                                                                                                               <  2:528294/251‑1 (MQ=255)                            gTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGa                                                                                                                                                                                                            <  1:605449/251‑1 (MQ=255)                                   ttcctgccctcGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACACCATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGttt                                                                                                                                                                                                     >  2:253277/9‑250 (MQ=255)                                             cggcagtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAgg                                                                                                                                                                                           <  1:253277/247‑1 (MQ=255)                                              ggcagtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGc                                                                                                                                                                                          <  1:308685/248‑1 (MQ=255)                                                  gtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGGATGCGCGTGAAGCTGGAAGGGAAAAACTAGATTGAGCAGTATGTTTATGCGTTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCGCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGa                                                                                                                                                                                      <  2:378465/251‑1 (MQ=255)                                                             ggtaagcttgcgcAGGCGCGGGAAAGTATTCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCg                                                                                                                                                                           <  2:337034/242‑1 (MQ=255)                                                              gtaagcttgcgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGt                                                                                                                                                                          <  1:543719/243‑1 (MQ=255)                                                               taagcttgcgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGCCTGGAGAATCGt                                                                                                                                                                          >  2:563694/8‑250 (MQ=255)                                                                          aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCCAAATGCGCGTATGCAGGCATTGGTAGAAACg                                                                                                                                                                                                        >  2:419215/1‑209 (MQ=255)                                                                          aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACg                                                                                                                                                                                                        <  1:419215/209‑1 (MQ=255)                                                                             cgcTGGAAAGTATGCGCGTGAAGCCGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAGCCCATGAGCTAAAAAGCCCACTTGCGGCGATTCGTGGCGCGTCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATGCTGACGCAAAATGCGCTTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTg                                                                                                                                                           <  2:533793/251‑1 (MQ=255)                                                                                    aaGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGActgttg                                                                                                                                                    >  1:248124/1‑248 (MQ=255)                                                                                    aaGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCAACTGGCGGCGATTCGTGGCGCGGCGGCAATTTTACGCGAAGGTCAGCCAACGGAAGTGGTGGGTAGTTTTACCGACAACATTCTGCCGCAAAATGCGCGTAGGCAGACATTGGTAGAAACTTTACTTCGCCAGGCACGGATGGAGAATCGTCAGGAAGACGATCTGActgttt                                                                                                                                                    >  2:54816/1‑248 (MQ=255)                                                                                           cgcgTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGAGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTAAGGAAGGCGTTCTGACTGTTGTTGAtgt                                                                                                                                             >  1:355346/1‑251 (MQ=255)                                                                                                 aaGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTAATACGCCAGGCAAGACTGGCGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggc                                                                                                                                       >  1:447361/1‑251 (MQ=255)                                                                                                      ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGCAATTTTACGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCCTTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGACGTCGGTCTGACTGTGGTTGATGTGGCGGCattat                                                                                                                                  >  2:221011/1‑251 (MQ=255)                                                                                                        aaGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                                                                                                                                <  1:414726/251‑2 (MQ=255)                                                                                                         aGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatttt                                                                                                                               >  1:492985/1‑249 (MQ=255)                                                                                                           ggAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTtcgc                                                                                                                             <  1:234819/251‑1 (MQ=255)                                                                                                              aaaaCTGTATTGAGCAGTATGTTTATGCGTTAACCAATGAGCTAAAAAGCCCACTGGCGGCGATTCGGGGCGCGGCGGAAATTTTAAGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTCTGCAGGCATTGGTAGAAACGATACTACGCCAGGAAAGACTGGAGAATCGTAAGGAAGTCGTTCTGACTGCTGTTGATGTggcggagttatttctccgc                                                                                                                          >  2:447449/1‑237 (MQ=255)                                                                                                                   gatatTGAGCAGTATGGTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATGCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAg                                                                                                                     <  2:28301/250‑1 (MQ=255)                                                                                                                            cAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTGACTACGCCGGGCAAGATTGGAGAATCGTCAGGAAGTCGTTCTGACGGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAgcgcg                                                                                                            <  1:285051/251‑1 (MQ=255)                                                                                                                               tATGTTTATGCGATAACCCATGAGCTAAAAAGACCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTTTTTCGCCCAGTCAGCGAAGCGGGCAc                                                                                                         >  1:445172/1‑251 (MQ=255)                                                                                                                                  gTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCg                                                                                                       <  1:88830/250‑1 (MQ=255)                                                                                                                                     tatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGc                                                                                                    >  1:375002/7‑250 (MQ=255)                                                                                                                                     tatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGGCTGGAGAATCGTCAGGAAGTCGGTCTGACTGTTGTTGATGTGGCGGCATTATTTTGCCGCGGCATCGGAGGGCGCACCGTGc                                                                                                    >  2:461128/7‑250 (MQ=255)                                                                                                                                      atgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCCAGACTGGAGAATCGTCAGGAAGTCGATCTGACAGTTGTTGATGTGGCGGACTTATTTCGCCGCGTCAACGAAGCGCGCACCGTGc                                                                                                    >  1:378778/6‑249 (MQ=255)                                                                                                                                       tgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTTTTTTCCCGCGTCAGCGAAgcgccccccctgcagg                                                                                                 >  2:537730/5‑239 (MQ=255)                                                                                                                                        gcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTGCGCCGCGTCAGCGAAGCGCGCACCGTGCAGtt                                                                                                <  1:537730/248‑1 (MQ=255)                                                                                                                                          gttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTgg                                                                                              <  1:116529/250‑1 (MQ=255)                                                                                                                                            tAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGAGTCAGCGAAGCGCGCTCCGGGCCGTTGGc                                                                                             >  1:185356/1‑250 (MQ=255)                                                                                                                                             aacccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAg                                                                                           <  2:362590/247‑1 (MQ=255)                                                                                                                                             aacccATGAGCGAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCa                                                                                            <  2:586990/246‑1 (MQ=255)                                                                                                                                                ccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCa                                                                                                                      <  1:587664/220‑1 (MQ=255)                                                                                                                                                 cATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAAGTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCa                                                                                                                                                                        <  2:246488/170‑1 (MQ=255)                                                                                                                                                  aTGAGCCAAAAAGCCCACAGGCGGCGGTTCGTGGCGCGGCGGGACTTTTAAGCGAAGGCCCGCCACCGGAAGTGGTGGCTCGTTTAACCGACAACAATCTGGCGCAAGATGCGCGTATGCACGCATTGGCAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCGGCCTGTTGTTGATGTGGAGGCAGTATTTCGCCGCGTCCACGAAGCGAGCGCCGTGCAGtttggctgaaa                                                                                       >  2:304411/1‑241 (MQ=255)                                                                                                                                                               cccACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCActctac                                                                         <  2:345607/251‑3 (MQ=255)                                                                                                                                                                      gcggcgATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTat                                                                  <  1:563694/251‑2 (MQ=255)                                                                                                                                                                                gaggcgctgcggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGTCAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTTATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAg                                                        <  2:355346/243‑1 (MQ=255)                                                                                                                                                                                gTGGCGCGGCGGAAATTTTACGCGCAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGGCGCAACATGCGCGTATGCAGGCATTGGTAGACACGTTACTACGCAAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCAATCTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGATGGCGGAAAAAGACATCACTCTCCATGTTATGccccctg                                                          >  1:254122/1‑245 (MQ=255)                                                                                                                                                                                gTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCGGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTACTTCGCCGCGTCCGCGCAGCGCGCACCGTGGAGTTGGCCGGAAAAAACATCACTCTACATGGTATGCCCActg                                                          >  2:503000/1‑247 (MQ=255)                                                                                                                                                                                 tggcgcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCTGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAACACATCCCTCTACATGttttgccccctgagg                                                       >  1:222434/5‑238 (MQ=255)                                                                                                                                                                                  ggcgcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGGTATGCCCACTGAGGt                                                      >  1:607296/4‑251 (MQ=255)                                                                                                                                                                                      cggcggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTaat                                                  >  2:181235/1‑250 (MQ=255)                                                                                                                                                                                          ggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGAGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCCGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGACGTTGGGAGAAAAAAAAATCACCCTACATGTTATGACCACTGAAGTTaatgatg                                              >  1:330188/1‑246 (MQ=255)                                                                                                                                                                                                    cgcgAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGCAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAAcc                                    >  2:456790/1‑251 (MQ=255)                                                                                                                                                                                                         aGGTCCGCCACCGGAAGTGGTGGCTCGTTTTGCCGACAACATTCTGACGCAGAATGCGCGTATGCAGGCATTGGTAGAAGCGTTACTACGCCAGGCAAGACTGGAGAATCGTCATGAAGGCGGTCTGACTGTTGTTGATGTGGTGGCATTAGTTCGCCGCGTGTGCGAAGCGCGCGCCGTGCGTTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGt                               <  1:309990/251‑1 (MQ=255)                                                                                                                                                                                                          ggTCCGCCACCGGAAGTGGTGGCTCGGTTTAACGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGCTAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTTCATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGtt                              <  2:375677/251‑1 (MQ=255)                                                                                                                                                                                                               gcaaccgGAAGTGGTTGCTCGTTTTAACGAAAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTTTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTgg                         <  2:445172/247‑1 (MQ=255)                                                                                                                                                                                                                    cgGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCTTTACttgggccg                    >  2:248915/1‑244 (MQ=255)                                                                                                                                                                                                                     ggAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                                                                                                                                >  1:147564/1‑141 (MQ=255)                                                                                                                                                                                                                     ggAAGGGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                                                                                                                                <  2:147564/142‑2 (MQ=255)                                                                                                                                                                                                                      gAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCCGCGAAGCGCGCACCGTGCAGTTGGCAGAAACAAACATCACTCTACATGTTATGCCCCCTGCGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAggg                  >  2:130333/1‑250 (MQ=255)                                                                                                                                                                                                                       aaGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCGGGCATTGGTAGAAACGTTACTCCTCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTGCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGGCAGCGAAGCGCGCTCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAAGGTTGCTGCTGAACCGTCGTTATTGGAGCAGgcg                 <  2:557048/251‑1 (MQ=255)                                                                                                                                                                                                                            gtggCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGCACCGGCGTTACTGGAGCAGGCGCTggg            >  1:388765/1‑251 (MQ=255)                                                                                                                                                                                                                             tggCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCAACGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACAGGCGTTACTGGAGCCGGCGCTgggg           >  2:466440/1‑250 (MQ=255)                                                                                                                                                                                                                                    ttttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGCAACGTTACTACGCCAGGCAAGACTGGAGACTCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCACCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTTCATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGTGAATTTAc    >  2:140084/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGCGCAGGCGCTGGGGAATTTACTg  >  1:238888/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTAAGGAAGTCGTAATGACTGTTGTTGATGAGGCGGCATTATTTCGCCGCGTAAGCGGAGAGCGCACCGTGCAGTTGGAAGAAACAAACACAACTCTACATGTTTTGCCCCCTGCGGTTAAAGTTGCTGCTGAACAGGCGTTACTGGAGCAGGGGCTggggagattaacg  >  2:226168/1‑241 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTG  >  NC_000913/4637344‑4637823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑