Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,553	G→A	P286P (CCG→CCA)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,553	0	G	A	100.0%	201.6 / NA	65	P286P (CCG→CCA)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/36);  total (29/36)

GGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTT  >  NC_000913/4637309‑4637795                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ggATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACGCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGaa                                                                                                                                                                                                                                              <  2:181201/251‑1 (MQ=255) ggATCAACCGCTCCATTCCCCGGCTCACTCGCTATGCCGATTCCGTCACTCACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGa                                                                                                                                                                                                                                               >  2:590521/1‑250 (MQ=255)       aCCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGCTTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAgtggtg                                                                                                                                                                                                                                        >  1:389666/1‑251 (MQ=255)                    ccGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGTAAGTGGTGGCTCGTTTTAccg                                                                                                                                                                                                                           <  1:456024/251‑3 (MQ=255)                       gCTCAATCGATATGCCGACTCCGTCACTGACAATCAGCCCGTTCCTCTTCCTGAAATCGGCAGTAGCGAGTTGCGTAAGCTTGCGACGGCGCTGGAAAGTATGCGCGTGACGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGCAGGTACGCCACCGGCAGGGGTGGCTCGTTTTAccgcc                                                                                                                                                                                                                         >  2:408735/1‑246 (MQ=255)                                    gccgATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCa                                                                                                                                                                                                           <  1:171631/248‑1 (MQ=255)                                      cgATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACGCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATGCTGACGCaaa                                                                                                                                                                                                         <  2:505262/250‑1 (MQ=255)                                           ccGTCCCTGACAATAAGTCCGTTCCTCTTCCTTAACTATGCAGTAGCAATGTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATACGCATGAAGCTGGAAGGTAAAAACTATAGTGAGCAGTATGTTTATGCGTTAACCCTTGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACCCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTGCCGACAACATTCTGACGCAAAATgct                                                                                                                                                                                                    <  1:436499/251‑2 (MQ=255)                                           ccGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATgcg                                                                                                                                                                                                    <  1:436511/251‑1 (MQ=255)                                            cGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATgcgc                                                                                                                                                                                                   <  1:175883/251‑1 (MQ=255)                                             gTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGCGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGcgcg                                                                                                                                                                                                  >  2:180501/1‑251 (MQ=255)                                                    aCAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACAGGAAGTGGTGGCTCGTTTTACCGACAACATTCTTACGCAAAATGCGCGTATGCAg                                                                                                                                                                                           <  1:491267/251‑1 (MQ=255)                                                      aaTAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTCTGCAGGc                                                                                                                                                                                         >  2:46855/1‑251 (MQ=255)                                                         aaGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCTCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGAGTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAtt                                                                                                                                                                                      <  1:607748/251‑1 (MQ=255)                                                         aaGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAtt                                                                                                                                                                                      <  2:389666/251‑1 (MQ=255)                                                          aGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTg                                                                                                                                                                                     <  1:130081/251‑1 (MQ=255)                                                            cccGGTCCTCTGCCTGAACTCGGCAGTAGCGAGTGGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACGGGCGGCGATTCGTGGCGCTGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACAGTCTGACGCAAAATGCGCGTAGGCAGGCATGGGt                                                                                                                                                                                   <  2:528294/251‑1 (MQ=255)                                                               gTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGa                                                                                                                                                                                <  1:605449/251‑1 (MQ=255)                                                                      ttcctgccctcGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACACCATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGttt                                                                                                                                                                         >  2:253277/9‑250 (MQ=255)                                                                                cggcagtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAgg                                                                                                                                                               <  1:253277/247‑1 (MQ=255)                                                                                 ggcagtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGc                                                                                                                                                              <  1:308685/248‑1 (MQ=255)                                                                                     gtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGGATGCGCGTGAAGCTGGAAGGGAAAAACTAGATTGAGCAGTATGTTTATGCGTTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCGCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGa                                                                                                                                                          <  2:378465/251‑1 (MQ=255)                                                                                                ggtaagcttgcgcAGGCGCGGGAAAGTATTCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCg                                                                                                                                               <  2:337034/242‑1 (MQ=255)                                                                                                 gtaagcttgcgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGt                                                                                                                                              <  1:543719/243‑1 (MQ=255)                                                                                                  taagcttgcgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGCCTGGAGAATCGt                                                                                                                                              >  2:563694/8‑250 (MQ=255)                                                                                                             aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCCAAATGCGCGTATGCAGGCATTGGTAGAAACg                                                                                                                                                                            >  2:419215/1‑209 (MQ=255)                                                                                                             aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACg                                                                                                                                                                            <  1:419215/209‑1 (MQ=255)                                                                                                                cgcTGGAAAGTATGCGCGTGAAGCCGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAGCCCATGAGCTAAAAAGCCCACTTGCGGCGATTCGTGGCGCGTCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATGCTGACGCAAAATGCGCTTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTg                                                                                                                               <  2:533793/251‑1 (MQ=255)                                                                                                                       aaGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGActgttg                                                                                                                        >  1:248124/1‑248 (MQ=255)                                                                                                                       aaGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCAACTGGCGGCGATTCGTGGCGCGGCGGCAATTTTACGCGAAGGTCAGCCAACGGAAGTGGTGGGTAGTTTTACCGACAACATTCTGCCGCAAAATGCGCGTAGGCAGACATTGGTAGAAACTTTACTTCGCCAGGCACGGATGGAGAATCGTCAGGAAGACGATCTGActgttt                                                                                                                        >  2:54816/1‑248 (MQ=255)                                                                                                                              cgcgTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGAGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTAAGGAAGGCGTTCTGACTGTTGTTGAtgt                                                                                                                 >  1:355346/1‑251 (MQ=255)                                                                                                                                    aaGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTAATACGCCAGGCAAGACTGGCGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggc                                                                                                           >  1:447361/1‑251 (MQ=255)                                                                                                                                         ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGCAATTTTACGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCCTTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGACGTCGGTCTGACTGTGGTTGATGTGGCGGCattat                                                                                                      >  2:221011/1‑251 (MQ=255)                                                                                                                                           aaGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                                                                                                    <  1:414726/251‑2 (MQ=255)                                                                                                                                            aGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatttt                                                                                                   >  1:492985/1‑249 (MQ=255)                                                                                                                                              ggAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTtcgc                                                                                                 <  1:234819/251‑1 (MQ=255)                                                                                                                                                 aaaaCTGTATTGAGCAGTATGTTTATGCGTTAACCAATGAGCTAAAAAGCCCACTGGCGGCGATTCGGGGCGCGGCGGAAATTTTAAGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTCTGCAGGCATTGGTAGAAACGATACTACGCCAGGAAAGACTGGAGAATCGTAAGGAAGTCGTTCTGACTGCTGTTGATGTggcggagttatttctccgc                                                                                              >  2:447449/1‑237 (MQ=255)                                                                                                                                                      gatatTGAGCAGTATGGTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATGCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAg                                                                                         <  2:28301/250‑1 (MQ=255)                                                                                                                                                               cAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTGACTACGCCGGGCAAGATTGGAGAATCGTCAGGAAGTCGTTCTGACGGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAgcgcg                                                                                <  1:285051/251‑1 (MQ=255)                                                                                                                                                                  tATGTTTATGCGATAACCCATGAGCTAAAAAGACCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTTTTTCGCCCAGTCAGCGAAGCGGGCAc                                                                             >  1:445172/1‑251 (MQ=255)                                                                                                                                                                     gTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCg                                                                           <  1:88830/250‑1 (MQ=255)                                                                                                                                                                        tatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGc                                                                        >  1:375002/7‑250 (MQ=255)                                                                                                                                                                        tatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGGCTGGAGAATCGTCAGGAAGTCGGTCTGACTGTTGTTGATGTGGCGGCATTATTTTGCCGCGGCATCGGAGGGCGCACCGTGc                                                                        >  2:461128/7‑250 (MQ=255)                                                                                                                                                                         atgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCCAGACTGGAGAATCGTCAGGAAGTCGATCTGACAGTTGTTGATGTGGCGGACTTATTTCGCCGCGTCAACGAAGCGCGCACCGTGc                                                                        >  1:378778/6‑249 (MQ=255)                                                                                                                                                                          tgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTTTTTTCCCGCGTCAGCGAAgcgccccccctgcagg                                                                     >  2:537730/5‑239 (MQ=255)                                                                                                                                                                           gcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTGCGCCGCGTCAGCGAAGCGCGCACCGTGCAGtt                                                                    <  1:537730/248‑1 (MQ=255)                                                                                                                                                                             gttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTgg                                                                  <  1:116529/250‑1 (MQ=255)                                                                                                                                                                               tAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGAGTCAGCGAAGCGCGCTCCGGGCCGTTGGc                                                                 >  1:185356/1‑250 (MQ=255)                                                                                                                                                                                aacccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAg                                                               <  2:362590/247‑1 (MQ=255)                                                                                                                                                                                aacccATGAGCGAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCa                                                                <  2:586990/246‑1 (MQ=255)                                                                                                                                                                                   ccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCa                                                                                          <  1:587664/220‑1 (MQ=255)                                                                                                                                                                                    cATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAAGTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCa                                                                                                                                            <  2:246488/170‑1 (MQ=255)                                                                                                                                                                                     aTGAGCCAAAAAGCCCACAGGCGGCGGTTCGTGGCGCGGCGGGACTTTTAAGCGAAGGCCCGCCACCGGAAGTGGTGGCTCGTTTAACCGACAACAATCTGGCGCAAGATGCGCGTATGCACGCATTGGCAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCGGCCTGTTGTTGATGTGGAGGCAGTATTTCGCCGCGTCCACGAAGCGAGCGCCGTGCAGtttggctgaaa                                                           >  2:304411/1‑241 (MQ=255)                                                                                                                                                                                                  cccACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCActctac                                             <  2:345607/251‑3 (MQ=255)                                                                                                                                                                                                         gcggcgATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTat                                      <  1:563694/251‑2 (MQ=255)                                                                                                                                                                                                                   gaggcgctgcggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGTCAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTTATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAg                            <  2:355346/243‑1 (MQ=255)                                                                                                                                                                                                                   gTGGCGCGGCGGAAATTTTACGCGCAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGGCGCAACATGCGCGTATGCAGGCATTGGTAGACACGTTACTACGCAAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCAATCTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGATGGCGGAAAAAGACATCACTCTCCATGTTATGccccctg                              >  1:254122/1‑245 (MQ=255)                                                                                                                                                                                                                   gTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCGGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTACTTCGCCGCGTCCGCGCAGCGCGCACCGTGGAGTTGGCCGGAAAAAACATCACTCTACATGGTATGCCCActg                              >  2:503000/1‑247 (MQ=255)                                                                                                                                                                                                                    tggcgcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCTGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAACACATCCCTCTACATGttttgccccctgagg                           >  1:222434/5‑238 (MQ=255)                                                                                                                                                                                                                     ggcgcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGGTATGCCCACTGAGGt                          >  1:607296/4‑251 (MQ=255)                                                                                                                                                                                                                         cggcggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTaat                      >  2:181235/1‑250 (MQ=255)                                                                                                                                                                                                                             ggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGAGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCCGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGACGTTGGGAGAAAAAAAAATCACCCTACATGTTATGACCACTGAAGTTaatgatg                  >  1:330188/1‑246 (MQ=255)                                                                                                                                                                                                                                       cgcgAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGCAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAAcc        >  2:456790/1‑251 (MQ=255)                                                                                                                                                                                                                                            aGGTCCGCCACCGGAAGTGGTGGCTCGTTTTGCCGACAACATTCTGACGCAGAATGCGCGTATGCAGGCATTGGTAGAAGCGTTACTACGCCAGGCAAGACTGGAGAATCGTCATGAAGGCGGTCTGACTGTTGTTGATGTGGTGGCATTAGTTCGCCGCGTGTGCGAAGCGCGCGCCGTGCGTTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGt   <  1:309990/251‑1 (MQ=255)                                                                                                                                                                                                                                             ggTCCGCCACCGGAAGTGGTGGCTCGGTTTAACGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGCTAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTTCATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGtt  <  2:375677/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTT  >  NC_000913/4637309‑4637795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑