Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,600	G→A	D120D (GAC→GAT)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,600	0	G	A	98.4%	178.1 / NA	63	D120D (GAC→GAT)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/29);  minor base C (1/0);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAAC  >  NC_000913/7355‑7834                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              cGGGCGACGCCATGAAGCCCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGTGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACg                                                                                                                                                                                                                                          <  1:463049/251‑1 (MQ=255)     cGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCt                                                                                                                                                                                                                                      <  1:419416/251‑1 (MQ=255)      gACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCtt                                                                                                                                                                                                                                     >  2:182650/1‑251 (MQ=255)           cATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGCAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCCTCCCGTTCTTtata                                                                                                                                                                                                                                 >  2:538903/1‑250 (MQ=255)                 gACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCtgt                                                                                                                                                                                                                          <  1:169284/251‑1 (MQ=255)                    cgcgAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCTGTCCGCCACGAAACTGCCCATTGGCATCACGtt                                                                                                                                                                                                                                        <  1:142612/234‑1 (MQ=255)                    cgcgAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGtt                                                                                                                                                                                                                                        >  2:142612/1‑234 (MQ=255)                       gAGTGATCGCCATCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTGCTTTATAACGCTGTGcgagg                                                                                                                                                                                                                    <  2:56730/251‑5 (MQ=255)                            atcgCCAGCAGAGCCAAGACAGCGAGAATAATACCTGTCAACAGCGGGGGAAAATCAAAAGAAAAACTCAGAGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCCTCCCCAGCCCGCGCGCCATATACCATGCAGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTCTAAAGCTGTGCGAGGGAAc                                                                                                                                                                                                                >  2:249570/4‑250 (MQ=255)                                     agagCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCCAACAGAACGCCCATCCAGCGCATCCCCATCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTCCTTTATAAAGCTGTGCTAGGGATCATTCGGCaaa                                                                                                                                                                                                      <  1:484529/251‑1 (MQ=255)                                      gagcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAAc                                                                                                                                                                                                     >  1:592607/5‑251 (MQ=255)                                       agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGATCGCGAAACGGAGTTCGCCTGAATTCCGCTGATAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCAAATACCAGGCCGGACCGCCACGAAACTGCCCATTGGCATCACGTTGTTTATAAAGCTGTGCGCGGGAACATTCAGCAAACg                                                                                                                                                                                                    >  1:277259/4‑251 (MQ=255)                                       agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACg                                                                                                                                                                                                    <  1:105825/248‑1 (MQ=255)                                            aGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTc                                                                                                                                                                                               <  2:200336/251‑1 (MQ=255)                                               cAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                                                                                                                            >  1:137501/1‑251 (MQ=255)                                                        aaataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                   <  1:370898/246‑1 (MQ=255)                                                         aataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCAGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGCCCGCCACGAAACTGCCCCTTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                   >  2:555721/5‑250 (MQ=255)                                                             ccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCGCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgc                                                                                                                                                                              <  1:319605/251‑1 (MQ=255)                                                              cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCACCGGCGTTAGCCTGAATTCCGCTGCAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGAATCCCCAGCCCGCGCGCCATATACCATGCCGGTACGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgcg                                                                                                                                                                             >  2:607514/1‑251 (MQ=255)                                                               tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGACGCCATGCCGATAAACGCgg                                                                                                                                                                            >  1:451862/1‑251 (MQ=255)                                                                             gAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGACCATCCAGCGAATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATcc                                                                                                                                                                >  1:583644/1‑249 (MQ=255)                                                                              aaaaTAAAAAGAAAAACCCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCGGAAAATTAGGCCGTAGGCGATGGGCAAAAAGACGGCGAACAGAACGCCCATCCAGCCCATCCCCAGCCCGCGCGCCTTATACCATGCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGCCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCaga                                                                                                                                                             <  2:519143/251‑3 (MQ=255)                                                                                   cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                        <  1:232223/251‑1 (MQ=255)                                                                                    aaaaGTAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACgg                                                                                                                                                       <  1:620114/251‑1 (MQ=255)                                                                                        aaaaaaCCCAGGGCTCGGCCAACGGCTTTCGCCTGAATGCCGCTGAAAATGATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCTCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGTTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAATACGGCTcc                                                                                                                                                   <  2:288932/250‑1 (MQ=255)                                                                                           aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAgg                                                                                                                                                >  1:483979/1‑251 (MQ=255)                                                                                           aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCAAgg                                                                                                                                                >  2:260682/1‑251 (MQ=255)                                                                                            aaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGt                                                                                                                                               >  2:548345/1‑251 (MQ=255)                                                                                             aCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTc                                                                                                                                              >  2:86900/1‑251 (MQ=255)                                                                                                cAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTcc                                                                                                                                             <  1:18816/249‑1 (MQ=255)                                                                                                       ggcgcagcGGCGTTCGCCTGAATCCCGCTAAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCATCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAATCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcg                                                                                                                                      <  2:195630/242‑1 (MQ=255)                                                                                                            aaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgag                                                                                                                                                                                                                     <  1:127960/165‑4 (MQ=255)                                                                                                            aaCGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgag                                                                                                                                                                                                                     >  2:127960/1‑162 (MQ=255)                                                                                                                     cgcctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCGCac                                                                                                                      >  2:279800/5‑250 (MQ=255)                                                                                                                                cGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAATTGACAGCGCAACGCCGGCCAgg                                                                                                           >  1:463767/1‑251 (MQ=255)                                                                                                                                  cTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGtt                                                                                                         >  1:87187/1‑251 (MQ=255)                                                                                                                                    gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                         <  1:531352/249‑1 (MQ=255)                                                                                                                                              ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTCCCGcgccccac                                                                                             >  2:350145/4‑244 (MQ=255)                                                                                                                                                   ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCACGCGCGCCATATACCATGCCGGTCCGCCGCGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGCGGGAACATTCGGCAAACGAGGTCGCCATGCCGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCAGCGGAACGCCGGCCCGGTTGACGGTACCCAcgcgac                                                                                        >  1:362641/1‑249 (MQ=255)                                                                                                                                                    gCGATGAGCAAAACGACGGCGAACAGAACGCCAATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGGTGCCGCCACCCACccgcgc                                                                                       >  1:499886/1‑251 (MQ=255)                                                                                                                                                        tGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGACGGCCAGGGTTCCGCCACCCACGGGcgacgc                                                                                    >  1:418753/1‑250 (MQ=255)                                                                                                                                                                  gACGGCGAACATACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTac                                                                        <  1:249570/251‑1 (MQ=255)                                                                                                                                                                   aCGGCGAACAGCACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGGTGCCGCTACCCCCGCGcgccgccacacagggacc                                                                        >  2:450849/1‑239 (MQ=255)                                                                                                                                                                    cGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTaca‑c                                                                     <  1:440603/251‑1 (MQ=255)                                                                                                                                                                      gCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑Caa                                                                   <  1:607514/251‑1 (MQ=255)                                                                                                                                                                        gAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAtt                                                                                                                                <  1:172086/190‑2 (MQ=255)                                                                                                                                                                                       ccAGCGCATCCCCAGCCCGCGCGCCATATCCCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTa                                                  <  2:159119/251‑1 (MQ=255)                                                                                                                                                                                        cAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGACATGAgggta                                                  >  1:79965/1‑247 (MQ=255)                                                                                                                                                                                           cgcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAAcc                                              <  1:623283/251‑1 (MQ=255)                                                                                                                                                                                            gcATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCg                                             <  1:383276/251‑1 (MQ=255)                                                                                                                                                                                            gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCCCGCGCGCCTCA‑AGACTGGGACA‑CACAGACTGAAATGAGGTTAAAccc                                             >  1:114212/1‑250 (MQ=255)                                                                                                                                                                                                ccccAGCCCCCGCGCCATAGCCCATGCCGGTCCGCCACGAAACTGCCCATTTGCACCCCTTTCTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTg                                         <  2:463767/251‑1 (MQ=255)                                                                                                                                                                                                           cccccaaaacccATGCCGGCCCGCCCCGAAACGGCCCATTGGCATCACTTTCTTGATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGa                             <  2:548305/241‑1 (MQ=255)                                                                                                                                                                                                                 tataCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCCGCGCCACGACGGCCCGGTTGCCGCTCCCCACGCCCGCCCCC‑CGCGTGGGACA‑CAATGACTTGAATGAGGGTAAAcccgccgggtgggggggcatgatt                        >  2:288852/1‑229 (MQ=255)                                                                                                                                                                                                                   aaCCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACGGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCGGTG‑GAGGAATGCTAtt                     <  2:116635/250‑1 (MQ=255)                                                                                                                                                                                                                    ccctggCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTTTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTAttt                    <  1:538903/246‑1 (MQ=255)                                                                                                                                                                                                                     ccATGGCGGTCCGACACGAAAATGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCACACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCCCCACCCACGCGCGCCGCA‑AGACTGGGACA‑CAAAGACTGAAATGTGGGTTAACCGCCCGGATGTG‑GGTGAATGCTAtttt                   >  2:619826/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTAttttt                  >  1:43479/1‑250 (MQ=255)                                                                                                                                                                                                                       aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATTATAtttttt                 >  1:94733/1‑250 (MQ=255)                                                                                                                                                                                                                          ccGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCACGCAACGCCGGCCAGGGTGCCGCCACCCACGCGCGCCGCA‑AGACTGGTACA‑CAACGAATGAACTGAGGGTAAACCGCCTGGCT‑TGTGATGAATGCTATTTTTAAgc              >  2:520448/1‑250 (MQ=255)                                                                                                                                                                                                                             gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTGATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACAACAA‑GAATTAAATGACGGTAAACCGCCTGGCTGGG‑GATGGATGCTATTTTTAAGACtt           >  1:125506/1‑251 (MQ=255)                                                                                                                                                                                                                               acGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAAAATTCGGCAAACGAGGACGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCGCGCCACGGCGGCAAGGATGCCCCTACCCACCCGCGCCGCACGGCCTGG‑ACA‑CCATGAATGCAATGAGGTTAAACCGCCTGTCTGTG‑GGTGAATACTATTTTTAAGACtttt         >  2:390620/2‑251 (MQ=255)                                                                                                                                                                                                                                      gAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATTCTATTTTTAAGACTTTTGCCAAAc  >  1:191775/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAAC  >  NC_000913/7355‑7834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑