Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,591	C→T	V195V (GTC→GTT)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,591	0	C	T	100.0%	201.0 / NA	62	V195V (GTC→GTT)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/28);  total (34/28)

GCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACG  >  NC_000913/4636351‑4636836                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gcgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCcac                                                                                                                                                                                                                                              >  2:24130/1‑250 (MQ=255)           cTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgtt                                                                                                                                                                                                                                   >  2:166902/1‑249 (MQ=255)            tGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                                                                                                  >  1:95485/1‑248 (MQ=255)              cgtgggttgaagaatgtcgcgtcGCCGTCCCCCGTCATCCGTATTGGTTATTTTGATTTAATTGAACCCGCGGCGCAAATCAGTTGT‑TTTGCCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGTTTCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGc                                                                                                                                                                                                                                 <  1:417476/228‑1 (MQ=255)               gTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATAAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGt                                                                                                                                                                                                                               >  2:391575/1‑251 (MQ=255)                         gaatttCTCGTCGCCGTCTCCCTTCATCCTTATTGGTCATTTTAATTTGAATGAACCCGCGGCGCAGATCAGCGGG‑TTTAACACGCCATTACCGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                                                                                                                                     <  1:397570/247‑1 (MQ=255)                         gaagtgccctcccccgcctcCCTTCATCCTTATCGGTTATTTTGAATGGAATGACCCCGCTGCGCAGAACA‑CTGGGTTTCACCCGCCATTAACGCTGACGCGGTATGAGTTCTTATTGCTGAAGACGTTACCCAAGCCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAACCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                                                                                                                                     <  1:338264/234‑1 (MQ=255)                                     gCCGTCGCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCCCAGATCAGCTGG‑TTTGACACGCCATTACCGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCAACATTAAAACGTTGCGTGCCAAGCTGCGCGCAATCAAcc                                                                                                                                                                                                         >  1:257142/1‑251 (MQ=255)                                         tctcCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCg                                                                                                                                                                                                      >  1:89430/1‑250 (MQ=255)                                            cccGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGctct                                                                                                                                                                                                  >  1:249341/1‑251 (MQ=255)                                            cccGTCATCCGTATGGGTCATTTTGAATTGAATGACCCCGCGGCGAAGATCAGTTGG‑TTTGACACGCCATTAACGCTGACGCGGTAGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCt                                                                                                                                                                                                  <  1:480694/251‑1 (MQ=255)                                                 cATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAc                                                                                                                                                                                             >  1:92914/1‑251 (MQ=255)                                                        aTTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGTCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGCTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTa                                                                                                                                                                                       >  2:143138/1‑250 (MQ=255)                                                          tggtcATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCGGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                                                                                                                                     >  1:431702/5‑250 (MQ=255)                                                                     aatttaatgaccctgcgtgccAGATCACCGGG‑TTTGACACGCCATTAACGCTGACCCGGTTGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCTCAGGACACCTACGATCGCTCCTTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCTCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGc                                                                                                                                                                         <  1:30458/231‑1 (MQ=255)                                                                         gaatgaaCCCGCGGCGAAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGATCTTTCACCGATTAATACTCATCGCGGCATgg                                                                                                                                                                     >  1:79234/1‑251 (MQ=255)                                                                                 ccGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGc                                                                                                                                                             >  1:130873/1‑251 (MQ=255)                                                                                    cggcgCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGTGATATAGCCTg                                                                                                                                                          >  2:346002/1‑251 (MQ=255)                                                                                         tagacacggtGG‑TTTGACCAGCCATTAGCGCTGCGGCGGTATGAGTTTGTATTGCGGAAGAGGTTATTCAA‑TCGGCCAGGCCGCTATTGGGACCGCCATCAACTAAGGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCGCATTAAAACGGTGTGTGCCAAGCTGCGTGCCATCAGCCCCGAGCTTTCGCCGATTAATACTCATCGCGGCATGGGATATAGCCTGAggg                                                                                                                                                      <  1:270683/241‑1 (MQ=255)                                                                                              cAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                                                                                >  2:154153/1‑251 (MQ=255)                                                                                                gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                                                                                                                                     >  2:43706/1‑212 (MQ=255)                                                                                                gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTaa                                                                                                                                              <  1:119306/251‑1 (MQ=255)                                                                                                  tGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGAGGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGAGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATAGCGGCATGGGATATAGCCTGAGGGGCCTGTAATg                                                                                                                                            >  1:304237/1‑251 (MQ=255)                                                                                                    g‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgcgcg                                                                                                                                                                                                                  >  1:119331/1‑179 (MQ=255)                                                                                                            acGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCCGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTACTGCGTATCGGc                                                                                                                                   >  1:560012/1‑251 (MQ=255)                                                                                                              gcctttaacgcggacgcGGTATGAGTTTTTAGTGCTAAAGACTTTACTCAAGTCG‑CCAGGCCGCGTCTGGGCCCGCCAGCAGCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                                                 <  2:339769/235‑1 (MQ=255)                                                                                                              gCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                                                 >  2:502871/1‑251 (MQ=255)                                                                                                                    aacgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                                                                                                                           >  1:130886/3‑251 (MQ=255)                                                                                                                      cgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgttgc                                                                                                                         >  1:107142/1‑250 (MQ=255)                                                                                                                          gacgcGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACTGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCGTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTgctgct                                                                                                                     <  1:391575/247‑1 (MQ=255)                                                                                                                                 tATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCGAGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAt                                                                                                              >  1:388960/1‑251 (MQ=255)                                                                                                                                   tGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt                                                                                                            >  1:361029/1‑251 (MQ=255)                                                                                                                                    gggTTTTTATGGCTGAAGACGTCACTCAGGTCG‑CCAGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTTTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt                                                                                                            <  2:95485/248‑1 (MQ=255)                                                                                                                                    gAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttt                                                                                                           <  1:154153/251‑1 (MQ=255)                                                                                                                                    gAGTTTTTATTGCTGAAGACGTTACTCAAGTAG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACAt                                                                                                                                                                                                                                            >  1:184260/1‑122 (MQ=255)                                                                                                                                               gctgAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATGTGTTACtggtgg                                                                                                 <  1:117809/248‑1 (MQ=255)                                                                                                                                                ctgAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcg                                                                                               >  1:510953/2‑251 (MQ=255)                                                                                                                                                  gAAGACGTTACTCAAGTCG‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcg                                                                                               <  1:421100/249‑1 (MQ=255)                                                                                                                                                        gtaaCTCAAGGCT‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcggtggcgg                                                                                        <  2:508178/247‑3 (MQ=255)                                                                                                                                                           aCTCAAGTCG‑CCAGGCCGCGTCTGGTCCCTCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTg                                                                                    <  1:273640/251‑1 (MQ=255)                                                                                                                                                               caaggcgccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGtt                                                                                 <  1:143138/241‑1 (MQ=255)                                                                                                                                                               aaGTCG‑CCAGGCTGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCTGGTCACCTACGATCGCACCGTTGATTCCCACATTAAAACGTTGCGCGCCAAGCTGCGCGCCATCAACCCCGATCTTGCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTc                                                                                <  2:578310/251‑1 (MQ=255)                                                                                                                                                                     gccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACt                                                                           <  1:24130/247‑1 (MQ=255)                                                                                                                                                                      ccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTg                                                                          >  1:455781/4‑251 (MQ=255)                                                                                                                                                                      ccaggCCGCGTCTGGTACCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTg                                                                          >  1:223290/4‑251 (MQ=255)                                                                                                                                                                               gtcgggTCCCGCCAGCAACTAATGGATAGCGGGTGGGAAGATGCGCAGGACACCGACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttttt                                                                 <  2:92914/247‑1 (MQ=255)                                                                                                                                                                                      cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATTCTTATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaaa                                                           <  2:220967/250‑1 (MQ=255)                                                                                                                                                                                        cGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACt                                                                                                                                                                                  >  1:341868/1‑129 (MQ=255)                                                                                                                                                                                        cGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACt                                                                                                                                                                                  <  2:341868/129‑1 (MQ=255)                                                                                                                                                                                               aaCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCTGCATGGGATATCGCCTGAGGGGCCTGTCATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGTCGGCCTGGTTCGTACCGGCTATTTTTGTCTAAGAAGTTAAAc                                                 >  1:305374/1‑251 (MQ=255)                                                                                                                                                                                                   aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCggg                                             <  2:204090/250‑1 (MQ=255)                                                                                                                                                                                                           gCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATCTAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCgc                                      >  2:429324/1‑249 (MQ=255)                                                                                                                                                                                                            cgtgtGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAAACGGGCGTGCGa                                      >  1:581836/5‑249 (MQ=255)                                                                                                                                                                                                                           gcatgACACCTACGATCGCACCGTTGAAACCCACATTAAAACGTTGCGTGCCAAGCTTCGCGCCATCAACCCCGATCTTTCACCGATTAATCCTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTCCTGCTGGGc                                                                                                                 <  2:611298/155‑1 (MQ=255)                                                                                                                                                                                                                           gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCACCCCCGATCTTTCACCGATTAATACTCATCGCGGCGTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGc                                                                                                                 >  1:611298/1‑159 (MQ=255)                                                                                                                                                                                                                           gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAAcc                                                <  1:452607/224‑1 (MQ=255)                                                                                                                                                                                                                           gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTGACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAc                     <  1:536207/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCg             >  1:588898/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCGACGGAGGGGACGTTAATCGATACCg       >  1:283836/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCg       <  1:478890/251‑1 (MQ=255)                                                                                                                                                                                                                                             cACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATGTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAAc   <  2:312264/251‑1 (MQ=255)                                                                                                                                                                                                                                              cccGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCGATTTTTGTCAAAGAAGTTAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACg  <  2:290044/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACG  >  NC_000913/4636351‑4636836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑