Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,555	A→G	V183V (GTA→GTG)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,555	0	A	G	100.0%	197.1 / NA	59	V183V (GTA→GTG)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/24);  total (35/24)

AACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGA  >  NC_000913/4636317‑4636800                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATgcg                                                                                                                                                                                                                                           >  1:329279/1‑251 (MQ=255)        ttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGac                                                                                                                                                                                                                                     >  1:328963/1‑250 (MQ=255)             cccGCGAAGTGTGCGCCAGTGTGCCCCCTTTCCTGCGTCGGGTAAAGAAGTTCTCGTCGCCGTCTCCCTTCATCCGTATTGGTCATTTTGAATGGAATGACCCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTGCTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTAc                                                                                                                                                                                                                               <  1:270189/251‑1 (MQ=255)                   aagtggcccccaggtggcgcCCCTTACTGGGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGCCACCCGATTTGGTATTTTTAAATTGAATGACCCCGGGGCCCAGATCAGTTGG‑TTTGACACGCCATTAACGCTGTCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGc                                                                                                                                                                                                                         <  1:460151/235‑1 (MQ=255)                    aGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCCTTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACg                                                                                                                                                                                                                              >  2:90094/1‑245 (MQ=255)                         gcgcCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGtt                                                                                                                                                                                                                   >  2:283526/1‑250 (MQ=255)                                   gcgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCcac                                                                                                                                                                                                          >  2:24130/1‑250 (MQ=255)                                             cTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgtt                                                                                                                                                                                               >  2:166902/1‑249 (MQ=255)                                              tGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                                                              >  1:95485/1‑248 (MQ=255)                                                cgtgggttgaagaatgtcgcgtcGCCGTCCCCCGTCATCCGTATTGGTTATTTTGATTTAATTGAACCCGCGGCGCAAATCAGTTGT‑TTTGCCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGTTTCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGc                                                                                                                                                                                             <  1:417476/228‑1 (MQ=255)                                                 gTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATAAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGt                                                                                                                                                                                           >  2:391575/1‑251 (MQ=255)                                                           gaatttCTCGTCGCCGTCTCCCTTCATCCTTATTGGTCATTTTAATTTGAATGAACCCGCGGCGCAGATCAGCGGG‑TTTAACACGCCATTACCGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                                                                                                 <  1:397570/247‑1 (MQ=255)                                                           gaagtgccctcccccgcctcCCTTCATCCTTATCGGTTATTTTGAATGGAATGACCCCGCTGCGCAGAACA‑CTGGGTTTCACCCGCCATTAACGCTGACGCGGTATGAGTTCTTATTGCTGAAGACGTTACCCAAGCCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAACCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                                                                                                 <  1:338264/234‑1 (MQ=255)                                                                       gCCGTCGCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCCCAGATCAGCTGG‑TTTGACACGCCATTACCGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCAACATTAAAACGTTGCGTGCCAAGCTGCGCGCAATCAAcc                                                                                                                                                                     >  1:257142/1‑251 (MQ=255)                                                                           tctcCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCg                                                                                                                                                                  >  1:89430/1‑250 (MQ=255)                                                                              cccGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGctct                                                                                                                                                              >  1:249341/1‑251 (MQ=255)                                                                              cccGTCATCCGTATGGGTCATTTTGAATTGAATGACCCCGCGGCGAAGATCAGTTGG‑TTTGACACGCCATTAACGCTGACGCGGTAGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCt                                                                                                                                                              <  1:480694/251‑1 (MQ=255)                                                                                   cATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAc                                                                                                                                                         >  1:92914/1‑251 (MQ=255)                                                                                          aTTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGTCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGCTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTa                                                                                                                                                   >  2:143138/1‑250 (MQ=255)                                                                                            tggtcATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCGGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                                                                                                 >  1:431702/5‑250 (MQ=255)                                                                                                       aatttaatgaccctgcgtgccAGATCACCGGG‑TTTGACACGCCATTAACGCTGACCCGGTTGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCTCAGGACACCTACGATCGCTCCTTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCTCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGc                                                                                                                                     <  1:30458/231‑1 (MQ=255)                                                                                                       aattgaatGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAg                                                                                                                                                                                                                                                >  2:453737/1‑144 (MQ=255)                                                                                                           gaatgaaCCCGCGGCGAAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGATCTTTCACCGATTAATACTCATCGCGGCATgg                                                                                                                                 >  1:79234/1‑251 (MQ=255)                                                                                                                   ccGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGc                                                                                                                         >  1:130873/1‑251 (MQ=255)                                                                                                                      cggcgCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGTGATATAGCCTg                                                                                                                      >  2:346002/1‑251 (MQ=255)                                                                                                                           tagacacggtGG‑TTTGACCAGCCATTAGCGCTGCGGCGGTATGAGTTTGTATTGCGGAAGAGGTTATTCAA‑TCGGCCAGGCCGCTATTGGGACCGCCATCAACTAAGGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCGCATTAAAACGGTGTGTGCCAAGCTGCGTGCCATCAGCCCCGAGCTTTCGCCGATTAATACTCATCGCGGCATGGGATATAGCCTGAggg                                                                                                                  <  1:270683/241‑1 (MQ=255)                                                                                                                                cAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                                            >  2:154153/1‑251 (MQ=255)                                                                                                                                  gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                                                                                                 >  2:43706/1‑212 (MQ=255)                                                                                                                                  gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTaa                                                                                                          <  1:119306/251‑1 (MQ=255)                                                                                                                                    tGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGAGGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGAGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATAGCGGCATGGGATATAGCCTGAGGGGCCTGTAATg                                                                                                        >  1:304237/1‑251 (MQ=255)                                                                                                                                      g‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgcgcg                                                                                                                                                                              >  1:119331/1‑179 (MQ=255)                                                                                                                                              acGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCCGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTACTGCGTATCGGc                                                                                               >  1:560012/1‑251 (MQ=255)                                                                                                                                                gcctttaacgcggacgcGGTATGAGTTTTTAGTGCTAAAGACTTTACTCAAGTCG‑CCAGGCCGCGTCTGGGCCCGCCAGCAGCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                             <  2:339769/235‑1 (MQ=255)                                                                                                                                                gCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                             >  2:502871/1‑251 (MQ=255)                                                                                                                                                      aacgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                                                                                       >  1:130886/3‑251 (MQ=255)                                                                                                                                                        cgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgttgc                                                                                     >  1:107142/1‑250 (MQ=255)                                                                                                                                                            gacgcGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACTGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCGTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTgctgct                                                                                 <  1:391575/247‑1 (MQ=255)                                                                                                                                                                   tATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCGAGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAt                                                                          >  1:388960/1‑251 (MQ=255)                                                                                                                                                                     tGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt                                                                        >  1:361029/1‑251 (MQ=255)                                                                                                                                                                      gggTTTTTATGGCTGAAGACGTCACTCAGGTCG‑CCAGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTTTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt                                                                        <  2:95485/248‑1 (MQ=255)                                                                                                                                                                      gAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttt                                                                       <  1:154153/251‑1 (MQ=255)                                                                                                                                                                      gAGTTTTTATTGCTGAAGACGTTACTCAAGTAG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACAt                                                                                                                                                                                                        >  1:184260/1‑122 (MQ=255)                                                                                                                                                                                 gctgAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATGTGTTACtggtgg                                                             <  1:117809/248‑1 (MQ=255)                                                                                                                                                                                  ctgAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcg                                                           >  1:510953/2‑251 (MQ=255)                                                                                                                                                                                    gAAGACGTTACTCAAGTCG‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcg                                                           <  1:421100/249‑1 (MQ=255)                                                                                                                                                                                          gtaaCTCAAGGCT‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTggtggcggtggcgg                                                    <  2:508178/247‑3 (MQ=255)                                                                                                                                                                                             aCTCAAGTCG‑CCAGGCCGCGTCTGGTCCCTCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTg                                                <  1:273640/251‑1 (MQ=255)                                                                                                                                                                                                 caaggcgccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGtt                                             <  1:143138/241‑1 (MQ=255)                                                                                                                                                                                                 aaGTCG‑CCAGGCTGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCTGGTCACCTACGATCGCACCGTTGATTCCCACATTAAAACGTTGCGCGCCAAGCTGCGCGCCATCAACCCCGATCTTGCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTc                                            <  2:578310/251‑1 (MQ=255)                                                                                                                                                                                                       gccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACt                                       <  1:24130/247‑1 (MQ=255)                                                                                                                                                                                                        ccaggCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTg                                      >  1:455781/4‑251 (MQ=255)                                                                                                                                                                                                        ccaggCCGCGTCTGGTACCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTg                                      >  1:223290/4‑251 (MQ=255)                                                                                                                                                                                                                 gtcgggTCCCGCCAGCAACTAATGGATAGCGGGTGGGAAGATGCGCAGGACACCGACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttttt                             <  2:92914/247‑1 (MQ=255)                                                                                                                                                                                                                        cccGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATTCTTATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCaaa                       <  2:220967/250‑1 (MQ=255)                                                                                                                                                                                                                          cGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACt                                                                                                                                              <  2:341868/129‑1 (MQ=255)                                                                                                                                                                                                                          cGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACt                                                                                                                                              >  1:341868/1‑129 (MQ=255)                                                                                                                                                                                                                                 aaCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCTGCATGGGATATCGCCTGAGGGGCCTGTCATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGTCGGCCTGGTTCGTACCGGCTATTTTTGTCTAAGAAGTTAAAc             >  1:305374/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCggg         <  2:204090/250‑1 (MQ=255)                                                                                                                                                                                                                                             gCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATCTAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCgc  >  2:429324/1‑249 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      AACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGA  >  NC_000913/4636317‑4636800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑