Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,493	T→C	L163L (TTG→CTG)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,493	0	T	C	100.0%	191.9 / NA	59	L163L (TTG→CTG)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/19);  total (40/19)

CCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTT  >  NC_000913/4636255‑4636731                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                ccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTACTGACGACTATGGGGCTAAACCGTTTTCACCCCGCGAAGTGAGCGCCAGGGTGCGCACCTTACTGCGTCGGGCGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGCATGGGTTTATATTGCTGAGGACGTTAc                                                                                                                                                                                                                                     >  2:492588/1‑251 (MQ=255)        gAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGt                                                                                                                                                                                                                               <  1:577571/250‑1 (MQ=255)           gagGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTcgcc                                                                                                                                                                                                                           >  1:12238/1‑248 (MQ=255)             ggTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTcgccag                                                                                                                                                                                                                         <  2:7311/251‑6 (MQ=255)              gTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTCAACCGTTTTCACACCGCGAAGTGTGCGCCAGGGGGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCCTTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTcgcccgg                                                                                                                                                                                                                        >  2:627592/1‑245 (MQ=255)                cgagcGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGcc                                                                                                                                                                                                                     <  1:350398/247‑1 (MQ=255)                 gATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCcg                                                                                                                                                                                                                    >  2:421100/1‑251 (MQ=255)                     cccTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTc                                                                                                                                                                                                                <  1:511383/250‑1 (MQ=255)                      ccTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTc                                                                                                                                                                                                                >  2:511383/1‑250 (MQ=255)                       cTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTg                                                                                                                                                                                                              >  1:204090/1‑251 (MQ=255)                           ttGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCCCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTc                                                                                                                                                                                                           <  2:117364/250‑1 (MQ=255)                            tGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCCGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTccc                                                                                                                                                                                                         >  2:303407/1‑251 (MQ=255)                             gggCTGGAAATTGGTGCTTACGACTATGTGGCTAACCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATGGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTccc                                                                                                                                                                                                         <  1:615555/250‑1 (MQ=255)                                    aaaTTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCa                                                                                                                                                                                                  >  1:312264/1‑250 (MQ=255)                                                    ctatgTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGtgt                                                                                                                                                                                 >  2:149188/5‑249 (MQ=255)                                                               aaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATgcg                                                                                                                                                                      >  1:329279/1‑251 (MQ=255)                                                                      ttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGac                                                                                                                                                                >  1:328963/1‑250 (MQ=255)                                                                           cccGCGAAGTGTGCGCCAGTGTGCCCCCTTTCCTGCGTCGGGTAAAGAAGTTCTCGTCGCCGTCTCCCTTCATCCGTATTGGTCATTTTGAATGGAATGACCCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTGCTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTAc                                                                                                                                                          <  1:270189/251‑1 (MQ=255)                                                                                 aagtggcccccaggtggcgcCCCTTACTGGGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGCCACCCGATTTGGTATTTTTAAATTGAATGACCCCGGGGCCCAGATCAGTTGG‑TTTGACACGCCATTAACGCTGTCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGc                                                                                                                                                    <  1:460151/235‑1 (MQ=255)                                                                                  aGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCCTTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACg                                                                                                                                                         >  2:90094/1‑245 (MQ=255)                                                                                       gcgcCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGtt                                                                                                                                              >  2:283526/1‑250 (MQ=255)                                                                                                 gcgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCcac                                                                                                                                     >  2:24130/1‑250 (MQ=255)                                                                                                  cgcACCTTACTGCGTCGGGTGAAGACGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGACTGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGcc                                                                                                                                                                                                      >  2:604425/1‑184 (MQ=255)                                                                                                           cTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgtt                                                                                                                          >  2:166902/1‑249 (MQ=255)                                                                                                            tGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                         >  1:95485/1‑248 (MQ=255)                                                                                                              cgtgggttgaagaatgtcgcgtcGCCGTCCCCCGTCATCCGTATTGGTTATTTTGATTTAATTGAACCCGCGGCGCAAATCAGTTGT‑TTTGCCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGTTTCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGc                                                                                                                        <  1:417476/228‑1 (MQ=255)                                                                                                               gTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATAAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGt                                                                                                                      >  2:391575/1‑251 (MQ=255)                                                                                                                     tGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATgg                                                                                                                                                                                          >  1:587863/1‑177 (MQ=255)                                                                                                                         gaatttCTCGTCGCCGTCTCCCTTCATCCTTATTGGTCATTTTAATTTGAATGAACCCGCGGCGCAGATCAGCGGG‑TTTAACACGCCATTACCGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                            <  1:397570/247‑1 (MQ=255)                                                                                                                         gaagtgccctcccccgcctcCCTTCATCCTTATCGGTTATTTTGAATGGAATGACCCCGCTGCGCAGAACA‑CTGGGTTTCACCCGCCATTAACGCTGACGCGGTATGAGTTCTTATTGCTGAAGACGTTACCCAAGCCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAACCACATTAAAACGTTGCGTGCCAAGCTgc                                                                                                            <  1:338264/234‑1 (MQ=255)                                                                                                                                     gCCGTCGCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCCCAGATCAGCTGG‑TTTGACACGCCATTACCGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCAACATTAAAACGTTGCGTGCCAAGCTGCGCGCAATCAAcc                                                                                                >  1:257142/1‑251 (MQ=255)                                                                                                                                         tctcCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCg                                                                                             >  1:89430/1‑250 (MQ=255)                                                                                                                                            cccGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGctct                                                                                         >  1:249341/1‑251 (MQ=255)                                                                                                                                            cccGTCATCCGTATGGGTCATTTTGAATTGAATGACCCCGCGGCGAAGATCAGTTGG‑TTTGACACGCCATTAACGCTGACGCGGTAGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCt                                                                                         <  1:480694/251‑1 (MQ=255)                                                                                                                                                 cATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCAc                                                                                    >  1:92914/1‑251 (MQ=255)                                                                                                                                                        aTTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGTCACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGCTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTa                                                                              >  2:143138/1‑250 (MQ=255)                                                                                                                                                          tggtcATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCGGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                            >  1:431702/5‑250 (MQ=255)                                                                                                                                                                     aatttaatgaccctgcgtgccAGATCACCGGG‑TTTGACACGCCATTAACGCTGACCCGGTTGGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCGGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCTCAGGACACCTACGATCGCTCCTTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCTCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGc                                                                <  1:30458/231‑1 (MQ=255)                                                                                                                                                                     aattgaatGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAg                                                                                                                                                                           >  2:453737/1‑144 (MQ=255)                                                                                                                                                                         gaatgaaCCCGCGGCGAAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCAGATCTTTCACCGATTAATACTCATCGCGGCATgg                                                            >  1:79234/1‑251 (MQ=255)                                                                                                                                                                                 ccGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGc                                                    >  1:130873/1‑251 (MQ=255)                                                                                                                                                                                    cggcgCAGATCAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGTGATATAGCCTg                                                 >  2:346002/1‑251 (MQ=255)                                                                                                                                                                                         tagacacggtGG‑TTTGACCAGCCATTAGCGCTGCGGCGGTATGAGTTTGTATTGCGGAAGAGGTTATTCAA‑TCGGCCAGGCCGCTATTGGGACCGCCATCAACTAAGGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGAGCGCACCGTTGATACCCGCATTAAAACGGTGTGTGCCAAGCTGCGTGCCATCAGCCCCGAGCTTTCGCCGATTAATACTCATCGCGGCATGGGATATAGCCTGAggg                                             <  1:270683/241‑1 (MQ=255)                                                                                                                                                                                              cAGCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                       >  2:154153/1‑251 (MQ=255)                                                                                                                                                                                                gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAAt                                                                            >  2:43706/1‑212 (MQ=255)                                                                                                                                                                                                gCTGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTaa                                     <  1:119306/251‑1 (MQ=255)                                                                                                                                                                                                  tGG‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGAGGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGAGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATAGCGGCATGGGATATAGCCTGAGGGGCCTGTAATg                                   >  1:304237/1‑251 (MQ=255)                                                                                                                                                                                                    g‑TTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTgcgcg                                                                                                         >  1:119331/1‑179 (MQ=255)                                                                                                                                                                                                            acGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCCGCAACTCATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTACTGCGTATCGGc                          >  1:560012/1‑251 (MQ=255)                                                                                                                                                                                                              gcctttaacgcggacgcGGTATGAGTTTTTAGTGCTAAAGACTTTACTCAAGTCG‑CCAGGCCGCGTCTGGGCCCGCCAGCAGCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                        <  2:339769/235‑1 (MQ=255)                                                                                                                                                                                                              gCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                        >  2:502871/1‑251 (MQ=255)                                                                                                                                                                                                                    aacgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgtt                  >  1:130886/3‑251 (MQ=255)                                                                                                                                                                                                                      cgcTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgttgc                >  1:107142/1‑250 (MQ=255)                                                                                                                                                                                                                          gacgcGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACTGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCGTGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTgctgct            <  1:391575/247‑1 (MQ=255)                                                                                                                                                                                                                                 tATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCGAGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAt     >  1:388960/1‑251 (MQ=255)                                                                                                                                                                                                                                   tGAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt   >  1:361029/1‑251 (MQ=255)                                                                                                                                                                                                                                    gggTTTTTATGGCTGAAGACGTCACTCAGGTCG‑CCAGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTTTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAttt   <  2:95485/248‑1 (MQ=255)                                                                                                                                                                                                                                    gAGTTTTTATTGCTGAAGACGTTACTCAAGTCG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtttt  <  1:154153/251‑1 (MQ=255)                                                                                                                                                                                                                                    gAGTTTTTATTGCTGAAGACGTTACTCAAGTAG‑CCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACAt                                                                                                                                   >  1:184260/1‑122 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                CCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGG‑TTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCA‑CCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTT  >  NC_000913/4636255‑4636731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑