Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,757	C→T	A79A (GCC→GCT)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,757	0	C	T	98.2%	175.6 / NA	55	A79A (GCC→GCT)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/22);  minor base G (0/1);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAG  >  NC_000913/4635518‑4635999                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   aaTATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTc                                                                                                                                                                                                                                          >  2:310764/1‑250 (MQ=255)   tatGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTAGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGc                                                                                                                                                                                                                                       >  1:522916/1‑251 (MQ=255)    atGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGc                                                                                                                                                                                                                                       >  1:197345/1‑250 (MQ=255)        aaTACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTc                                                                                                                                                                                                                                  >  1:419632/1‑251 (MQ=255)           aCAAGCATTTGATCCTGTCTTTAAACCTGATAATGCTGGGGCCATTGGATCATGCAGAAGAGATTGGTTCGGACGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTCTTTCTTGTCAGCAAGTCggg                                                                                                                                                                                                                               >  1:151184/1‑251 (MQ=255)            cAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGc                                                                                                                                                                                                                              <  2:622964/251‑1 (MQ=255)                       tCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACATGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGCAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTg                                                                                                                                                                                                                   >  2:226245/1‑251 (MQ=255)                               ttAAGCCTGATAATGCTGTGGCCATTGGCTCATGCAGAATAGATTGGTGCGATCGTCACCGTATTGAAAATGATCGGCGCGGATCACAAAATTGTTGTGGAAGACTGTGACGATCCCGATGTGAAAAATGACACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTAGTAAAGGGGGAGTGGGTCTGGCGGAAGATACCTCCGATGCGGCGATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATc                                                                                                                                                                                                            <  2:230497/250‑1 (MQ=255)                               ttAAGCCTGATAATGCTGGGGCCAGTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAAGTGTTGTGGAAGCCGTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGAt                                                                                                                                                                                                             <  2:325479/249‑1 (MQ=255)                                tAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGTTCGt                                                                                                                                                                                                          >  2:198970/1‑251 (MQ=255)                                    ccTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTAtt                                                                                                                                                                                                       >  2:322586/1‑250 (MQ=255)                                     cTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTa                                                                                                                                                                                                      >  1:499041/1‑250 (MQ=255)                                                 gggCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGc                                                                                                                                                                                         >  1:240213/1‑251 (MQ=255)                                                   gCCATAGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGGATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTACAAACGGCAAAGCTc                                                                                                                                                                                       >  2:592637/1‑251 (MQ=255)                                                      aTTGGCTCATGCAGAAGAGCTTGGTTAGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAACATTGTTGAGGAAGACTTTGACGATACCGATGTGAAAACTGTCAACTGTTATGTGAGCCGGGCGTACACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTACGATGCGGCTATTTCTTGTCAGCCAGTCGGACCGATTGAACTGTAGGATCGCATTCAAAACGGCAAAGCTCAgg                                                                                                                                                                                    >  1:277831/1‑251 (MQ=255)                                                          gCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCg                                                                                                                                                                                 >  1:301744/1‑250 (MQ=255)                                                             cATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGgt                                                                                                                                                                             <  1:463326/251‑1 (MQ=255)                                                                gCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACTGCAAAGCTCAGGGCGAGgtag                                                                                                                                                                           <  1:527042/250‑1 (MQ=255)                                                                    aagaGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCGGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTc                                                                                                                                                                      <  2:103694/251‑1 (MQ=255)                                                                        gaTTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGGCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCaaaa                                                                                                                                                                  <  1:615399/251‑1 (MQ=255)                                                                             gTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGc                                                                                                                                                             >  1:566822/1‑251 (MQ=255)                                                                             gTTCGGTCAACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGc                                                                                                                                                             <  2:449529/251‑1 (MQ=255)                                                                                     gACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCt                                                                                                                                                     >  1:546515/1‑251 (MQ=255)                                                                                       cacCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTgg                                                                                                                                                   <  2:499041/251‑1 (MQ=255)                                                                                           gTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCtt                                                                                                                                               <  1:550220/251‑1 (MQ=255)                                                                                                  aaaTGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCg                                                                                                                                        >  1:136470/1‑251 (MQ=255)                                                                                                      gATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTa                                                                                                                                     <  2:197345/250‑1 (MQ=255)                                                                                                         cGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAgg                                                                                                                                 >  1:547477/1‑251 (MQ=255)                                                                                                           gCCCGGATCACGAAATTGTTGTGCGAGCATTTGACGATCCCGATGAGAAAAATGTAAACTGTTATGTGCGCCGGGCGAAAACAGGCGGTATTAAAGAGGGCTTAGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCCAGTCGGGCCGATTGACCTGTCGGATCGTCTTCAAACCGGCAAAGCTCAGGGCGCGGTAGAATTCAAATAACGCACGACCCTGGTCTTTAAGTCGTTACAGGTc                                                                                                                               >  2:627172/1‑251 (MQ=255)                                                                                                              cGGATCACAAAATTGTTGTGGAAGCCTTTGGCGAATCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTAGTAAAGGGGGAGTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGTATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTg                                                                                                                            <  2:544093/251‑1 (MQ=255)                                                                                                              cGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTg                                                                                                                            <  2:457123/251‑1 (MQ=255)                                                                                                               ggATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGCAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTgc                                                                                                                           >  2:185055/1‑251 (MQ=255)                                                                                                                gATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTgcg                                                                                                                          >  1:352171/1‑251 (MQ=255)                                                                                                                gATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTAGTTACAGGTCGTgcg                                                                                                                          >  1:528296/1‑251 (MQ=255)                                                                                                                gATCACAAAATTGTTGGGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGGATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCGGGTCTTTAAGTCGTTACAGGTCGTgcg                                                                                                                          <  2:252056/251‑1 (MQ=255)                                                                                                                                           gacgatCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGcgct                                                                                               <  1:382272/248‑1 (MQ=255)                                                                                                                                           gacgatCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGGCGGGCGCTTTTATGACGCCAAACGCAACGcgct                                                                                               <  2:180033/248‑1 (MQ=255)                                                                                                                                                           aaaaTGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAAAGCGCCCGCTTATCTGGCTTAc                                                                               >  2:354792/1‑251 (MQ=255)                                                                                                                                                                gTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCg                                                                           >  1:521662/1‑250 (MQ=255)                                                                                                                                                                         tATGTGAGCAGGGCGAAAACAGGTGGTATTAAAGGGGGCTTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGt                                                                 >  2:254670/1‑251 (MQ=255)                                                                                                                                                                               aGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAACGTTGTAGAAgg                                                           >  2:508113/1‑251 (MQ=255)                                                                                                                                                                                  cGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTAGTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACTTCCCTGGTCTTTAAGTCGTTACAGGGCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTc                                                        <  2:301744/251‑1 (MQ=255)                                                                                                                                                                                                 ggtATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGAt                                         <  1:60170/251‑1 (MQ=255)                                                                                                                                                                                                   aaTTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGGCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGAtt                                        >  2:132115/2‑250 (MQ=255)                                                                                                                                                                                                        aaGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTCGAAGGTTCGCCGAAAAACGCGATTAGcgcg                                  >  1:123948/1‑251 (MQ=255)                                                                                                                                                                                                                ttGGGTCTGGCGGAAGATACCTCCGCAGCGGCTATTTAATGTCAGCACGACGGGCCGATTGCACTGCCGGCTCGTATTACAAACCCCAAAGCCCAGGGCGGGAGAGTATTCAAAAAACGCACGTCACTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAAAGCGCTCGATCATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGt                          >  2:599189/1‑251 (MQ=255)                                                                                                                                                                                                                  gggTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCa                        <  2:540417/251‑1 (MQ=255)                                                                                                                                                                                                                  gggTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCGTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTc                         <  2:402017/250‑1 (MQ=255)                                                                                                                                                                                                                    gTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGAAAGTCGGGCCGATTGAACTGTCGGCTCGTATTAAAAACGGCAAAGCTAAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTAAAGGCCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTAATCCGACAAAGATGTAGAAGGTTCGCCGAAAAACGCGATAAGCGCGGTTACTGTCATg                      >  1:293747/1‑251 (MQ=255)                                                                                                                                                                                                                         gCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTg                 <  1:24098/251‑1 (MQ=255)                                                                                                                                                                                                                         gCGGAAGATACCTCCGATGAGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGACTTAAAAACGGCAAAGCTCAGGGCGCGGTAGTATTCAAACAACGCACGTCCCTGGACTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAACACGCGATTAGCGCGGTTCCTGTCATGCCGTg                 >  2:270189/1‑251 (MQ=255)                                                                                                                                                                                                                             aaGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTggcgg             >  2:310030/1‑251 (MQ=255)                                                                                                                                                                                                                                  cccatggATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGGCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATa        <  1:4952/245‑1 (MQ=255)                                                                                                                                                                                                                                      ccGATGCAGCTATTTCTTGTCAGCAAGTCGTGCCGCTTGTACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGATCTTTAAGTCGTTACAGGTCGTGCGCGTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTCCTCCGACAACGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCCTGCCGTGGCGTCAATAACa     >  2:381401/1‑250 (MQ=255)                                                                                                                                                                                                                                        gATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGAGCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACagag  <  2:240213/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   AATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAG  >  NC_000913/4635518‑4635999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑