Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,323	A→T	T197T (ACA→ACT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,323	0	A	T	99.0%	306.3 / NA	96	T197T (ACA→ACT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (55/40);  minor base G (0/1);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTC  >  NC_000913/4631081‑4631567                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         ggcatGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTTATATGCAAAATGGAACTCCGGGCAGAGTGAAGAAGGCTGGCAAGTGGCGAAAGAGCTGTTGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTATTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGTCGTATTTAGAGCGTATCCTTCTGGCGATGAAAGTGGTTAACTCTGGCCTgg                                                                                                                                                                                                                                                   <  2:531239/246‑1 (MQ=255)        tAGCCTTTAGACCGGCAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGACACGCAGACTGGCAGGGGGCGAAGGAGCTGTGGCTAACCGGCCAGAGCCAGCCTAACGCCTGTCACAAGTTATTTAG‑GACTTGGCGTGCGTCAGGTAAAAAAGATCCACTGGCGTATTTAGAGCGTATCTGTCTGGCGATGACAGCGGGTAACAATGGCATGGGAACAGTg                                                                                                                                                                                                                                           >  2:238129/1‑251 (MQ=255)         aGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTg                                                                                                                                                                                                                                           <  2:47884/250‑1 (MQ=255)         aGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGc                                                                                                                                                                                                                                          >  1:49483/1‑251 (MQ=255)          gCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCt                                                                                                                                                                                                                                         <  1:476874/251‑1 (MQ=255)              ttAGCCCGGAAAAGCCTGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGCGAAGAAGCCTGGCAAGGGTCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAACCAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACAATGGCCTGGTAAAAGTGCTggcg                                                                                                                                                                                                                                     >  2:138285/1‑250 (MQ=255)                     ggAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagggc                                                                                                                                                                                                                                 >  2:239014/1‑248 (MQ=255)                             ccGGATCTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcctgcc                                                                                                                                                                                                                      <  1:15678/251‑1 (MQ=255)                              cGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCTAAAGAGCTGTTGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGTTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATTAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCg                                                                                                                                                                                                                     <  2:279866/251‑1 (MQ=255)                                    tACCGAAGCGCTATGAAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTACCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAc‑c                                                                                                                                                                                                              >  2:320900/1‑251 (MQ=255)                                      ccGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAg                                                                                                                                                                                                            <  2:521594/251‑1 (MQ=255)                                        gAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGAc                                                                                                                                                                                                          >  1:118091/1‑251 (MQ=255)                                             gcAATGTAATTCCTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCg                                                                                                                                                                                                     <  2:441530/251‑1 (MQ=255)                                               aaTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGcc                                                                                                                                                                                                   >  1:166680/1‑251 (MQ=255)                                                   taattaatACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGAGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCGCgg                                                                                                                                                                                               >  2:203593/1‑251 (MQ=255)                                                    aaTTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCgg                                                                                                                                                                                               <  1:337716/250‑1 (MQ=255)                                                      ttACTACTCTGTGAACTGGACCACCGGGCAGAGTCCAGCAGCCTGGTAAGCGGCGAAAGAGCTGTGGCTAACCGGAAAGAGCCAGCCAAACGCCTGTGACAAGCTATTTAGTGTCT‑GGCGTGCGTCAGGAACACAAGATCCGCTGGCGTATTTAGAGCGTATCAGTCTGGCGATGAAAGCGGGAAACACTGGCCTGGTAACAGGGCTGGCAGGGCAGATGCCTGCCGATTCC‑CACCCTATCGCCTCGGCa                                                                                                                                                                                             >  2:549104/1‑250 (MQ=255)                                                       tactacTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAAt                                                                                                                                                                                           >  1:352607/1‑251 (MQ=255)                                                        actactATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTCACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCCCGGCAATc                                                                                                                                                                                          >  2:610322/1‑251 (MQ=255)                                                            ctaTGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCAttt                                                                                                                                                                                      >  1:4818/1‑251 (MQ=255)                                                                  aaaaTGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTgg                                                                                                                                                                                >  1:241097/2‑251 (MQ=255)                                                                       ggAACACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aac                                                                                                                                                                          >  1:175115/1‑251 (MQ=255)                                                                        gcactccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGATTAGGTATTGAGTGGCT‑GGCGTGCGTTAGGTAAACAAGATCCGCTGGCGTATTTAGAGCTTATCCGTCTGGCGATGAAAGCGGGTACTACTGGCCTGGTAACAGTGCTGGCACAGCAGATGCCGGCCGATTAC‑CAGAATATCGCCTCGGTGATCATTTCTCTGGCG‑aaca                                                                                                                                                                         <  2:394608/246‑1 (MQ=255)                                                                        gAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aac                                                                                                                                                                          >  1:169938/1‑250 (MQ=255)                                                                         aaCACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aacaa                                                                                                                                                                        <  1:67418/251‑1 (MQ=255)                                                                            acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGTGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTCGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCATGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                                                                                                     >  2:358468/1‑251 (MQ=255)                                                                            acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                                                                                                     >  1:381807/1‑251 (MQ=255)                                                                            acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                                                                                                     >  1:77550/1‑251 (MQ=255)                                                                             ccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTCC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCt                                                                                                                                                                    >  2:544056/1‑251 (MQ=255)                                                                             ccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCt                                                                                                                                                                    >  1:515846/1‑251 (MQ=255)                                                                                  cAGAGCGAAGAAGCCTGGCAAGGGGAGACAGAGCTGTGGCTAACAGGCAAGAGCCAGCCTAACGCCTGTGAAAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGCTCAGCTGGCGTATTTAGAGCGTATCCGACTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGAAATCATTTCACTGGCG‑AACAACCCTAATAc                                                                                                                                                               >  2:625752/1‑251 (MQ=255)                                                                                   agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                                                                                                              <  2:49483/251‑1 (MQ=255)                                                                                   agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                                                                                                              >  1:285188/1‑251 (MQ=255)                                                                                   agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCGGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                                                                                                              <  2:218713/251‑1 (MQ=255)                                                                                        gaagaaGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACt                                                                                                                                                         >  1:209265/1‑251 (MQ=255)                                                                                         aagaagTCTGGCACGGGACGACAGAGCTGTGGCTCACCGGCAAGACCCAGCCTAACGCCTGTGACCAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTCGAGCGTATCCGTCTGGCGATGAAAGCGGGTCACACTGGCCTGGTAAACGGGCTGGCAGGCCAGATGCTCGCCGATTACACA‑ACACTCGCCTCGGCCATCATTTCACCCGCG‑AACACCCATAATACGGTACTg                                                                                                                                                        >  2:266549/1‑251 (MQ=255)                                                                                         aagaagCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTg                                                                                                                                                        >  1:359965/1‑251 (MQ=255)                                                                                           gaagCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGAc                                                                                                                                                      <  1:89210/251‑1 (MQ=255)                                                                                               ccTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTc                                                                                                                                                  <  1:242461/251‑1 (MQ=255)                                                                                                  ggCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTcgcg                                                                                                                                               <  1:495698/251‑1 (MQ=255)                                                                                                    cAAGGGGCGAAAGAGCTGTGGCTAACCGGCACGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTcgcgcg                                                                                                                                             >  2:215227/1‑251 (MQ=255)                                                                                                     aagtggCGAAAGGGCGGGGGGTAACCGGTAAGAGCCATCCGAACACCTGTGACAAGTTATTTATTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCGGGCAGGGCAGATGCCGGCCTATTTT‑CAGACTATCGCCTCGGGAATCATTTCATGGGCG‑AAGACCCCTAATACGGTACGGAGCTGCGCGCGt                                                                                                                                            <  2:263240/247‑1 (MQ=255)                                                                                                     aaGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGt                                                                                                                                            <  1:94000/251‑1 (MQ=255)                                                                                                     aaGGGGCGAAAGAGCGTTGGCTAACCGGCAAGCGCCAGCCTAACGACTGTGACAAGTTATGTATTGTCT‑GGCGTGCGTCAGGCAAACAAGATACGCAGGCGTATTTAGAGCGTATCAGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCAGCCATCATTTCAC‑GGCGGCACAACCCTAATAAGGGACTGACCATcacgcgg                                                                                                                                            >  2:18839/1‑250 (MQ=255)                                                                                                              aaGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGgc                                                                                                                                   <  2:74062/251‑1 (MQ=255)                                                                                                                    tgtgGCTAACCGGCCAGAGCCAGACTCCCGCCTGTGACAAGTTATTTCGTGTCT‑GGCGGGCGCCCGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGAAACAGTGCTGGCCGGGCAGATGCCTGCCGATTAC‑CAGACTATCACCTCGGCAATCCTTTCACTGGCG‑AAACACCCTAATACGGTACTGACATTCGCGCGTACAACTGGCGCGAcc                                                                                                                             >  2:231380/1‑251 (MQ=255)                                                                                                                    tgtgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGgcgagccc                                                                                                                             >  2:94838/1‑246 (MQ=255)                                                                                                                     gtgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGAc                                                                                                                              <  1:543928/249‑1 (MQ=255)                                                                                                                       ggCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGAGTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACGGGCGCGACCGAt                                                                                                                          <  2:322262/251‑1 (MQ=255)                                                                                                                        gCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTAGTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACCGACCTTCGCGCGTTCAACTGGCGCGACCGAtt                                                                                                                         <  1:499037/251‑1 (MQ=255)                                                                                                                         cTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACACCTGGCGCGACCGAttt                                                                                                                        >  2:17891/1‑251 (MQ=255)                                                                                                                          tAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGCTTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGGACAACTGGCGCGACCGAtttt                                                                                                                       >  2:181255/1‑251 (MQ=255)                                                                                                                            aCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAc                                                                                                                     >  1:276868/1‑251 (MQ=255)                                                                                                                                 cAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTc                                                                                                               <  1:8536/251‑1 (MQ=255)                                                                                                                                  aaGAGCCCGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCGCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCa                                                                                                              <  2:352607/251‑1 (MQ=255)                                                                                                                                           ccTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATggcggc                                                                                                     >  1:317845/1‑251 (MQ=255)                                                                                                                                            cTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGgcggcg                                                                                                    >  2:598108/1‑251 (MQ=255)                                                                                                                                              aaCGCCTGTGACTCGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACCACTGGCGCGACCGATTTTAC‑CCGTCAACTGGcggcggg                                                                                                  >  2:35188/1‑250 (MQ=255)                                                                                                                                                cGCCTGTGACAAGTTAGTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCGGGCGTATTTATAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCGGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAAGCATTTCACTTGCG‑AACAACCCGAATACGGGAGTGACCTGCGCGCGTACAACTGGCTCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTgg                                                                                                <  2:569795/251‑1 (MQ=255)                                                                                                                                                              tATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGtgtg                                                                                 >  1:466055/1‑251 (MQ=255)                                                                                                                                                               aTTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTCTTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAggtggg                                                                                >  1:150108/1‑246 (MQ=255)                                                                                                                                                                tttAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAACTGGCGGCGGTGGCGTTT‑GCCCGTGTGgc                                                                               >  1:415881/1‑251 (MQ=255)                                                                                                                                                                        tcaggagtgcgtCAGGTAAACCAGATCCGCTGGCGTATTTAAAGCGCATCCGTCTGGCGATGCAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑CACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTAACATGGAGGCGGTGGCGTTT‑GCCAGGGTGGCGCGGc                                                                          >  2:409494/7‑249 (MQ=255)                                                                                                                                                                        cT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGGGGCGCGGCAgg                                                                       >  2:581233/1‑251 (MQ=255)                                                                                                                                                                         t‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAggg                                                                      >  2:539839/1‑250 (MQ=255)                                                                                                                                                                           ggCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGAACTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAATGTGGCGCGGCAGGAt                                                                     >  1:511827/1‑251 (MQ=255)                                                                                                                                                                             cgtgcgtCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGc                                                                   <  1:436972/251‑1 (MQ=255)                                                                                                                                                                             agtgcgtCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCCATCATTTCACTGTCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGGGGCGTTT‑GCCCGTGTGGCCCGGctggcggt                                                                   >  2:9515/2‑244 (MQ=255)                                                                                                                                                                              gtgggtCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGGGCGGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAAGCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAAGGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCt                                                                  <  1:404568/247‑1 (MQ=255)                                                                                                                                                                              gtgcgtCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTCCTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGGTGCt                                                                  >  2:561409/1‑251 (MQ=255)                                                                                                                                                                                    cAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGaa                                                             <  1:181255/250‑1 (MQ=255)                                                                                                                                                                                    cAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAAt                                                            >  1:193150/1‑251 (MQ=255)                                                                                                                                                                                    cAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTAGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGAATATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATCCGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGaa                                                             <  1:432519/250‑1 (MQ=255)                                                                                                                                                                                     aGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATg                                                           >  1:501540/1‑251 (MQ=255)                                                                                                                                                                                      ggTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGATGCGTTTTGCC‑GTGTGGCGCGGGAgggagctgggaaagg                                                          >  2:495205/1‑238 (MQ=255)                                                                                                                                                                                               gATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgat                                                 >  1:569120/1‑251 (MQ=255)                                                                                                                                                                                                  ccGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCtgatta                                               <  2:352840/250‑3 (MQ=255)                                                                                                                                                                                                   cGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCCATCATTTCACTGGCG‑AACAACCCTAATACGGTACt                                                                                                                                                         >  1:254307/1‑145 (MQ=255)                                                                                                                                                                                                   cGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATAAC‑CAGACGATCGCCTCGGCAATCAGTTCACTGGCG‑AACATCCCTAATACGGTACt                                                                                                                                                         <  2:254307/145‑1 (MQ=255)                                                                                                                                                                                                      tGGCGTATTTCGAGCGTATCCGTCTGGCGATGACAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCCGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGGGGGGGTT‑GCCAGTGTGGCGGGGCAGTATGGTGAGAATGACCGGCTGATGATccc                                           >  2:101540/1‑250 (MQ=255)                                                                                                                                                                                                        gCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATc                                        <  2:501540/251‑1 (MQ=255)                                                                                                                                                                                                            aTTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCtt                                    >  1:22367/1‑251 (MQ=255)                                                                                                                                                                                                            aTTTAGAGCGTATCCGGGTGGCGATGAAAGCGGGGAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAGGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGAGTGTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGcg                                     <  2:13658/250‑2 (MQ=255)                                                                                                                                                                                                                agagCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGAAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATACCATCGCTTGccc                                >  1:68385/1‑251 (MQ=255)                                                                                                                                                                                                                 gagCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCa                               <  2:515846/251‑1 (MQ=255)                                                                                                                                                                                                                      tATCCGTCTGGCTACGAAAGCGGGTAACGCTGTCGTGGTAACAGTGCTGGCAGGGCAGATGCTCGCTGATTAT‑CTGACTAGCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTTGCGCGACCGATTTTACTCCG‑CAAATTGCGGCGGGGGCGTTT‑GTCAGTGTGGCGCGGCAGGATGCTCAGAATGCTCGTCtgaataccacagcgcgtgcgcaggc                            <  2:598618/249‑23 (MQ=255)                                                                                                                                                                                                                         ccGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcagc                       <  2:193150/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCgcagca                      <  2:438430/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCGTGCCCAGGCgcagca                      <  1:371684/251‑1 (MQ=255)                                                                                                                                                                                                                               ggCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCagcagc                    <  1:561409/248‑1 (MQ=255)                                                                                                                                                                                                                               ggCGATGAAAGCGGGTAACACGGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTa                 <  1:608584/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGAAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCagcagc                    <  1:281540/244‑1 (MQ=255)                                                                                                                                                                                                                                   aTGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGGGGCGCGGAAGGATGCTGGGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCagcagc                    >  2:281540/1‑244 (MQ=255)                                                                                                                                                                                                                                      aaaGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGGGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGGGCAGCAGCTTAATGa             >  2:397978/1‑248 (MQ=255)                                                                                                                                                                                                                                         gCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCa       <  1:359764/251‑1 (MQ=255)                                                                                                                                                                                                                                              tAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCCGTGTGGCGCGGCAGGGTGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCCGGCGCCGCCGCTTAATGGAGATCAGATTc  >  1:218003/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         GGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAC‑CCGTCAAATGGCGGCGGTGGCGTTT‑GCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTC  >  NC_000913/4631081‑4631567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑