Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,245	C→T	S171S (AGC→AGT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,245	0	C	T	98.9%	256.4 / NA	88	S171S (AGC→AGT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/36);  minor base G (1/0);  total (52/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGC  >  NC_000913/4631000‑4631490                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         cgcgCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑g                                                                                                                                                                                                                                                    <  2:41029/251‑1 (MQ=255) cgcgCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCGTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑g                                                                                                                                                                                                                                                    <  2:522668/251‑1 (MQ=255)   cgcTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTAATACTATGCAAAATGGGACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGGGTCT‑ggg                                                                                                                                                                                                                                                  >  1:388540/1‑250 (MQ=255)   cccgacacgccgctcgtccgatctccGCACGCTGCAATCTCGTTTCGACAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAAGGTAATTACTACTAGGCAAAATGGAACACCGGGCAGAGAGAAGAAGCCTGGCAAGGGGCGAAAAAGCGGTGGCTAACCGGCAAGAGCCAGCCGAACTCCTGTGACAAGGTATTTAGTGTCT‑Ggc                                                                                                                                                                                                                                                  <  2:36703/226‑1 (MQ=255)       aaCCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGCAGCGCCATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑Ggcgtgc                                                                                                                                                                                                                                              >  1:47884/1‑251 (MQ=255)       aaCCCCACGCTTCCTCACGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑Ggcgtgc                                                                                                                                                                                                                                              >  1:141539/1‑251 (MQ=255)        aCCCCACGCTTCCTGCCGCCCGGACGCTGGAATCTCGGTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACGCCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTGACGCCTGTGACAACTTAGTTAGTTTCT‑tgcgtgcg                                                                                                                                                                                                                                             <  2:144396/251‑1 (MQ=255)        aCCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGACGACTGGCGTGGCTT‑GTTAACCTTTAGCCCGGAGAAGCCCGGAACTACCGCAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGCAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAAAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTTT‑Ggggtgcg                                                                                                                                                                                                                                             >  2:359764/1‑251 (MQ=255)               gCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTATTTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGATGTGGCTAACCGGAAAGAGCCAGCCTAACGCCTGTGACAAGGTATTTAATGGCT‑GGCGTGCGGCAGGt                                                                                                                                                                                                                                       >  2:436972/1‑250 (MQ=255)                 ttCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGGACAACGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGATAACCGGCAAGCGCCCGCCTAACGCCTGTGACACGTTATTTAGTGTCT‑GGCGTGCGTCAGGTaa                                                                                                                                                                                                                                     >  1:569795/1‑250 (MQ=255)                      cccGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCCAGGGGCGCAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTCCCGCCTGTGACAAGTTATTTAGTGTCT‑TGCGTGCGTCAGGTAAACa                                                                                                                                                                                                                                  >  1:13658/1‑248 (MQ=255)                      cccGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAg                                                                                                                                                                                                                                <  1:72015/250‑1 (MQ=255)                         gCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGGAAACAAGATcc                                                                                                                                                                                                                            >  1:477671/1‑251 (MQ=255)                           tCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAAACTGGCGTGGCTT‑GGTAACCTTTAGGCCGGAAACCCCCGGATCTACCGACGCGCAATGGAATTACTACTATGCACACTGGGACACCCGGCCGGGCGAAGAAGCCTGGCAAGGGGCGAAAGGGCTGTGGGTAACCGGCAAGCGCCAGCCTAACGCCTGTGGCCAGTTATTTTGTGTCT‑Ggcagatcggaagaacacacgtccta                                                                                                                                                                                                                           >  1:36703/1‑227 (MQ=255)                             gCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTg                                                                                                                                                                                                                        <  1:283955/251‑1 (MQ=255)                              cACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGTCAGGCGTGGCTT‑GTTATCCATTACACCGACAAAGCCCGGAACTGCCGAAGCGCCATGTAATTACTACTATGCAAAATGGAACACCGGGCTGAGTGAAGAAACCTGGCAAGGGGCGAACGAACTGTGGCTTACCGACAAGAGCCAGCCTCCCGCCTGTGGCACGTTGTTTCATGTCT‑GGCGTGCGTCATGTCAACAAGATCCGCTgg                                                                                                                                                                                                                       >  1:394608/1‑251 (MQ=255)                                    gCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTa                                                                                                                                                                                                                   <  1:188852/249‑1 (MQ=255)                                     cAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGGAAACAAGATCCGCTGGCGTAttt                                                                                                                                                                                                                >  2:467769/1‑251 (MQ=255)                                       aTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTAttt                                                                                                                                                                                                                >  1:438430/1‑249 (MQ=255)                                               tcttCAATGAACTGGCTCGGCGTGAATACCGGCGTGGCTTTGTT‑GTCTTTAGCCCGGAAAAGCTCTGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGATGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCGAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGAGTCAGGTAAACAAGATCCTCTGGAGTATTTAGAGCTTAt                                                                                                                                                                                                       <  2:399653/247‑1 (MQ=255)                                                        aaCTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCg                                                                                                                                                                                             <  2:161471/251‑1 (MQ=255)                                                         aCTGGCGCGGCGTGAAGACTGGAGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACAAACGAAGCACAATGTACTTACTACTCTGCCAAATGGACCACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCCAACCGGCAAAAGCCAGCCTAAAGCCAGGGAACAGTTATTTAGTGTCT‑GGCGGGCGTCAGGGAAACAAAAAACGCGGGAGGATTTAGAGGGTATCCGTCTGGCg                                                                                                                                                                                             >  2:293260/1‑250 (MQ=255)                                                            ggCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTACCCGTCTGGCGATg                                                                                                                                                                                          <  2:10290/250‑1 (MQ=255)                                                                cggcgTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGc                                                                                                                                                                                     <  1:521943/251‑1 (MQ=255)                                                                                  ggcat‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTTATATGCAAAATGGAACTCCGGGCAGAGTGAAGAAGGCTGGCAAGTGGCGAAAGAGCTGTTGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTATTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGTCGTATTTAGAGCGTATCCTTCTGGCGATGAAAGTGGTTAACTCTGGCCTgg                                                                                                                                                                    <  2:531239/246‑1 (MQ=255)                                                                                          tAGCCTTTAGACCGGCAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGACACGCAGACTGGCAGGGGGCGAAGGAGCTGTGGCTAACCGGCCAGAGCCAGCCTAACGCCTGTCACAAGTTATTTAG‑GACTTGGCGTGCGTCAGGTAAAAAAGATCCACTGGCGTATTTAGAGCGTATCTGTCTGGCGATGACAGCGGGTAACAATGGCATGGGAACAGTg                                                                                                                                                            >  2:238129/1‑251 (MQ=255)                                                                                           aGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTg                                                                                                                                                            <  2:47884/250‑1 (MQ=255)                                                                                           aGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGc                                                                                                                                                           >  1:49483/1‑251 (MQ=255)                                                                                            gCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCt                                                                                                                                                          <  1:476874/251‑1 (MQ=255)                                                                                                ttAGCCCGGAAAAGCCTGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGCGAAGAAGCCTGGCAAGGGTCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAACCAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACAATGGCCTGGTAAAAGTGCTggcg                                                                                                                                                      >  2:138285/1‑250 (MQ=255)                                                                                                       ggAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagggc                                                                                                                                                  >  2:239014/1‑248 (MQ=255)                                                                                                               ccGGATCTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcctgcc                                                                                                                                       <  1:15678/251‑1 (MQ=255)                                                                                                                cGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCTAAAGAGCTGTTGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGTTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATTAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCg                                                                                                                                      <  2:279866/251‑1 (MQ=255)                                                                                                                 ggAACTACCGAAGCGCAATGTAATTACTACTATGCAAACTGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCg                                                                                                                                                                                    >  2:492782/1‑204 (MQ=255)                                                                                                                 ggAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCg                                                                                                                                                                                    <  1:492782/204‑1 (MQ=255)                                                                                                                      tACCGAAGCGCTATGAAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTACCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAc‑c                                                                                                                               >  2:320900/1‑251 (MQ=255)                                                                                                                        ccGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAg                                                                                                                             <  2:521594/251‑1 (MQ=255)                                                                                                                          gAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGAc                                                                                                                           >  1:118091/1‑251 (MQ=255)                                                                                                                               gcAATGTAATTCCTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCg                                                                                                                      <  2:441530/251‑1 (MQ=255)                                                                                                                                 aaTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGcc                                                                                                                    >  1:166680/1‑251 (MQ=255)                                                                                                                                     taattaatACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGAGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCGCgg                                                                                                                >  2:203593/1‑251 (MQ=255)                                                                                                                                      aaTTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCgg                                                                                                                <  1:337716/250‑1 (MQ=255)                                                                                                                                        ttACTACTCTGTGAACTGGACCACCGGGCAGAGTCCAGCAGCCTGGTAAGCGGCGAAAGAGCTGTGGCTAACCGGAAAGAGCCAGCCAAACGCCTGTGACAAGCTATTTAGTGTCT‑GGCGTGCGTCAGGAACACAAGATCCGCTGGCGTATTTAGAGCGTATCAGTCTGGCGATGAAAGCGGGAAACACTGGCCTGGTAACAGGGCTGGCAGGGCAGATGCCTGCCGATTCC‑CACCCTATCGCCTCGGCa                                                                                                              >  2:549104/1‑250 (MQ=255)                                                                                                                                         tactacTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAAt                                                                                                            >  1:352607/1‑251 (MQ=255)                                                                                                                                          actactATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTCACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCCCGGCAATc                                                                                                           >  2:610322/1‑251 (MQ=255)                                                                                                                                              ctaTGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCAttt                                                                                                       >  1:4818/1‑251 (MQ=255)                                                                                                                                                    aaaaTGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTgg                                                                                                 >  1:241097/2‑251 (MQ=255)                                                                                                                                                         ggAACACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aac                                                                                           >  1:175115/1‑251 (MQ=255)                                                                                                                                                          gcactccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGATTAGGTATTGAGTGGCT‑GGCGTGCGTTAGGTAAACAAGATCCGCTGGCGTATTTAGAGCTTATCCGTCTGGCGATGAAAGCGGGTACTACTGGCCTGGTAACAGTGCTGGCACAGCAGATGCCGGCCGATTAC‑CAGAATATCGCCTCGGTGATCATTTCTCTGGCG‑aaca                                                                                          <  2:394608/246‑1 (MQ=255)                                                                                                                                                          gAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aac                                                                                           >  1:169938/1‑250 (MQ=255)                                                                                                                                                           aaCACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑aacaa                                                                                         <  1:67418/251‑1 (MQ=255)                                                                                                                                                              acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGTGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTCGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCATGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                      >  2:358468/1‑251 (MQ=255)                                                                                                                                                              acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                      >  1:381807/1‑251 (MQ=255)                                                                                                                                                              acCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAAccc                                                                                      >  1:77550/1‑251 (MQ=255)                                                                                                                                                               ccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTCC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCt                                                                                     >  2:544056/1‑251 (MQ=255)                                                                                                                                                               ccGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCt                                                                                     >  1:515846/1‑251 (MQ=255)                                                                                                                                                                    cAGAGCGAAGAAGCCTGGCAAGGGGAGACAGAGCTGTGGCTAACAGGCAAGAGCCAGCCTAACGCCTGTGAAAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGCTCAGCTGGCGTATTTAGAGCGTATCCGACTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGAAATCATTTCACTGGCG‑AACAACCCTAATAc                                                                                >  2:625752/1‑251 (MQ=255)                                                                                                                                                                     agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                               <  2:49483/251‑1 (MQ=255)                                                                                                                                                                     agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                               >  1:285188/1‑251 (MQ=255)                                                                                                                                                                     agagTGAAGAAGCCTGGCAAGGGGCGAAAGAGCGGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACg                                                                               <  2:218713/251‑1 (MQ=255)                                                                                                                                                                          gaagaaGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACt                                                                          >  1:209265/1‑251 (MQ=255)                                                                                                                                                                           aagaagTCTGGCACGGGACGACAGAGCTGTGGCTCACCGGCAAGACCCAGCCTAACGCCTGTGACCAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTCGAGCGTATCCGTCTGGCGATGAAAGCGGGTCACACTGGCCTGGTAAACGGGCTGGCAGGCCAGATGCTCGCCGATTACACA‑ACACTCGCCTCGGCCATCATTTCACCCGCG‑AACACCCATAATACGGTACTg                                                                         >  2:266549/1‑251 (MQ=255)                                                                                                                                                                           aagaagCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTg                                                                         >  1:359965/1‑251 (MQ=255)                                                                                                                                                                             gaagCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGAc                                                                       <  1:89210/251‑1 (MQ=255)                                                                                                                                                                                 ccTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTc                                                                   <  1:242461/251‑1 (MQ=255)                                                                                                                                                                                    ggCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTcgcg                                                                <  1:495698/251‑1 (MQ=255)                                                                                                                                                                                      cAAGGGGCGAAAGAGCTGTGGCTAACCGGCACGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTcgcgcg                                                              >  2:215227/1‑251 (MQ=255)                                                                                                                                                                                       aagtggCGAAAGGGCGGGGGGTAACCGGTAAGAGCCATCCGAACACCTGTGACAAGTTATTTATTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCGGGCAGGGCAGATGCCGGCCTATTTT‑CAGACTATCGCCTCGGGAATCATTTCATGGGCG‑AAGACCCCTAATACGGTACGGAGCTGCGCGCGt                                                             <  2:263240/247‑1 (MQ=255)                                                                                                                                                                                       aaGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGt                                                             <  1:94000/251‑1 (MQ=255)                                                                                                                                                                                       aaGGGGCGAAAGAGCGTTGGCTAACCGGCAAGCGCCAGCCTAACGACTGTGACAAGTTATGTATTGTCT‑GGCGTGCGTCAGGCAAACAAGATACGCAGGCGTATTTAGAGCGTATCAGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCAGCCATCATTTCAC‑GGCGGCACAACCCTAATAAGGGACTGACCATcacgcgg                                                             >  2:18839/1‑250 (MQ=255)                                                                                                                                                                                                aaGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGgc                                                    <  2:74062/251‑1 (MQ=255)                                                                                                                                                                                                      tgtgGCTAACCGGCCAGAGCCAGACTCCCGCCTGTGACAAGTTATTTCGTGTCT‑GGCGGGCGCCCGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGAAACAGTGCTGGCCGGGCAGATGCCTGCCGATTAC‑CAGACTATCACCTCGGCAATCCTTTCACTGGCG‑AAACACCCTAATACGGTACTGACATTCGCGCGTACAACTGGCGCGAcc                                              >  2:231380/1‑251 (MQ=255)                                                                                                                                                                                                      tgtgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGgcgagccc                                              >  2:94838/1‑246 (MQ=255)                                                                                                                                                                                                       gtgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGAc                                               <  1:543928/249‑1 (MQ=255)                                                                                                                                                                                                         ggCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGAGTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACGGGCGCGACCGAt                                           <  2:322262/251‑1 (MQ=255)                                                                                                                                                                                                          gCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTAGTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACCGACCTTCGCGCGTTCAACTGGCGCGACCGAtt                                          <  1:499037/251‑1 (MQ=255)                                                                                                                                                                                                           cTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACACCTGGCGCGACCGAttt                                         >  2:17891/1‑251 (MQ=255)                                                                                                                                                                                                            tAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGCTTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGGACAACTGGCGCGACCGAtttt                                        >  2:181255/1‑251 (MQ=255)                                                                                                                                                                                                              aCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAc                                      >  1:276868/1‑251 (MQ=255)                                                                                                                                                                                                                   cAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGAGCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTc                                 <  1:8536/251‑1 (MQ=255)                                                                                                                                                                                                                    aaGAGCCCGCCTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCGCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCa                                <  2:352607/251‑1 (MQ=255)                                                                                                                                                                                                                             ccTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATggcggc                       >  1:317845/1‑251 (MQ=255)                                                                                                                                                                                                                              cTAACGCCTGTGACAAGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGgcggcg                      >  2:598108/1‑251 (MQ=255)                                                                                                                                                                                                                                aaCGCCTGTGACTCGTTATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACCACTGGCGCGACCGATTTTACCCGTCAACTGGcggcggg                    >  2:35188/1‑250 (MQ=255)                                                                                                                                                                                                                                  cGCCTGTGACAAGTTAGTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCGGGCGTATTTATAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCGGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAAGCATTTCACTTGCG‑AACAACCCGAATACGGGAGTGACCTGCGCGCGTACAACTGGCTCGACCGATTTTACCCGTCAAATGGCGGCGGTgg                  <  2:569795/251‑1 (MQ=255)                                                                                                                                                                                                                                                tATTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGtgtg    >  1:466055/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aTTTAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTCTTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAggtggg   >  1:150108/1‑246 (MQ=255)                                                                                                                                                                                                                                                  tttAGTGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAACTGGCGGCGGTGGCGTTTGCCCGTGTGgc  >  1:415881/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         CGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTT‑GTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCT‑GGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAC‑CAGACTATCGCCTCGGCAATCATTTCACTGGCG‑AACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGC  >  NC_000913/4631000‑4631490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑