| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,630,984 | G→T | T84T (ACG→ACT) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,630,984 | 0 | G | T | 98.7% | 242.6 / NA | 78 | T84T (ACG→ACT) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (39/38); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTG > NC_000913/4630742‑4631229 | gCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTa > 1:284774/1‑250 (MQ=255) ccAAACACGTTACCTGGCGAATGTTGGCAGCCGGTGTCAGTCTGCTGACGGTCAGCAGCGTGCCACGAGCCGCCTCACTGGATAAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCAGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGCTGACCTGATGAATCAACCGGCCGTGACTGTAACTaa > 2:72015/1‑250 (MQ=255) aGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTc < 1:320131/251‑1 (MQ=255) aCCTGGCGGCTGTTGGCTGCCGGTGGCTGTCGGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTcgcg < 2:449159/250‑1 (MQ=255) tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTACCTTTGTTCGCGCTa > 2:353574/1‑250 (MQ=255) tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTa > 1:171047/1‑250 (MQ=255) ggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAcccc < 1:238210/251‑1 (MQ=255) ggCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtc < 1:602573/251‑1 (MQ=255) acGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGAGACTGTCACTAACTTTGTTCGCGCTAACAACACGCTTcccccc > 2:8536/2‑251 (MQ=255) tgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAATGTCACTAACTTTGTTCAAGCTAACCCCACGCTTCCTCCCGCTCgaac > 1:598618/1‑248 (MQ=255) gtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc < 2:497847/250‑1 (MQ=255) ctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGc > 1:41029/1‑250 (MQ=255) agcGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGt > 2:476874/1‑251 (MQ=255) gcGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTc < 1:353574/251‑1 (MQ=255) gTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCaa < 1:495373/251‑1 (MQ=255) cgAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACt > 2:521943/1‑251 (MQ=255) ccGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgcg < 2:550589/251‑1 (MQ=255) cACTGGACGAGCAGCTGAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCGGGACTGAAAGATTAGCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACGTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTTGCACGCTGCAATCTCGTATCGTGAATGAACTGGCGCGGCGt < 2:569550/251‑1 (MQ=255) gATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTACCTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCGCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGTGGCGTGAAGa < 2:403109/251‑1 (MQ=255) aTGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGAc > 1:521594/1‑251 (MQ=255) agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGACTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAATGTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGt > 1:578106/1‑251 (MQ=255) cgtagccggtaagcgaaaaTCAAGCAGGCATGGGATAATCGCCAAATGGATGTGGTCGAACAAATGAAGCCTGGACTGAAAGACTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGCTCGCGCAAACCCCACACTCCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGTACTGGCGCGGCGTGAAGACCGGCGTgg > 2:89210/17‑251 (MQ=255) gTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGc > 1:279866/1‑251 (MQ=255) tagccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGTTGTGGTCGCACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCt > 2:543928/5‑251 (MQ=255) agccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCtt < 1:138440/248‑1 (MQ=255) gTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGATTGTCCCTACCTTTGTTCGCGCTAACCCAAAGGTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGAACTGGCgcggactggaggaccacgtgggtgtttt > 2:144351/1‑227 (MQ=255) tACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCGGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCTGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGGGGCTTGTTAg < 2:256425/250‑1 (MQ=255) gcgcAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑tt < 1:75359/251‑1 (MQ=255) cgcAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCCCGGCGTGACGAATGGCGAGGCtttgttggcttt > 2:596917/1‑241 (MQ=255) cAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCg < 1:585568/250‑1 (MQ=255) gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCgg < 1:520226/250‑1 (MQ=255) gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCgg < 1:278301/250‑1 (MQ=255) gggATAATCGACAAATGGAAGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGCTGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCTTGTTTGCC‑CTTTGCCCCGAAAAACCCgg > 2:371684/1‑250 (MQ=255) aTAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc < 1:156315/177‑1 (MQ=255) aTAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc > 2:156315/1‑177 (MQ=255) tAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGCAGACTGGCGTGGCTTGTTTGCC‑TTTTGCCCGGGAAAGCCCGGAACtt > 2:337716/1‑250 (MQ=255) aCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGAGGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCTTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCACGGAACTTCCGAAg < 2:291558/251‑1 (MQ=255) aCAAATGGATGTGGTCGAACAAATGAGGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAg < 1:42303/251‑1 (MQ=255) aaaTGGATGTGGTCGAACAAATGATGCCTGGACGGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAAGGAACTGGCTCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAgcg < 2:618271/251‑1 (MQ=255) ggATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAAt < 2:159175/251‑1 (MQ=255) tgGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTGGCCCTTTA‑CCCGGAAAAGCCCGGGACTACCGAAGCGCAATGGAAt > 2:188852/1‑251 (MQ=255) ggTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAAt < 1:118196/250‑1 (MQ=255) ggTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGGTAGGC‑TTTAGCCCGGGAAAACACGGAACTACCCAAGCGCAATGGAAtt > 1:218713/1‑251 (MQ=255) gtagAACAAATGAGGCCTGGACTGAAAGATTATCCCCTTTATCCCGTCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGTCTGTCACTAACTTTGTTCGCGCTAACCCCTCGCTTCCTCCCGCTCGCACGCTTCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAATC‑TTTAGCCCGGAAAAGCCCGGAACTGCCTAAGCGCAATGTAATta < 2:404373/248‑1 (MQ=255) caaaCAAATGATGCCTGGACTGAAAGATTAGCCACTTTATCCCTTCCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCTGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCGCTCACTCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCTTGTTAGCC‑TTTATCCCGGAAAAGCCCGGAACTCCCAAAGCGGAGTCTAATtac < 2:33838/248‑1 (MQ=255) cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATtact < 1:171494/251‑1 (MQ=255) cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATtac < 1:185384/250‑1 (MQ=255) aaCAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACACCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTACC‑TTTAGCCCGGAAAAGCCCCGAACCAACCAAGCGCAAattaattaattc > 2:495698/1‑247 (MQ=255) aaTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGACTACCGTCAGGTCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCAAGCTTCCTCCCGCTCGCCCGCTGCAATCACGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCC‑CTTAGTCCGGCAAAGCCCGGAACTACCGAAGCCCGATGAAAATACTACT‑ATg > 2:608584/1‑251 (MQ=255) aaTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGACTACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCCAAGCGCCATGTAATTCCTACT‑Att > 1:263240/1‑250 (MQ=255) atgatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCCATGTAATTACTACT‑ATGc > 1:336652/1‑251 (MQ=255) atgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCGTGTTAACC‑CTTAGCCCGGAAAAGCCCGGAACTACCCAAGCGCCATGGAATTACTCCT‑ATGcaa > 2:88055/1‑248 (MQ=255) cTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAACCCGGAACTACCGCAGCGCCATGTAATTACTACT‑ATGCAAAATgg > 1:322262/1‑251 (MQ=255) ggACTGAAAGATTATCCTCTTTATCCCTCCCTGGAATACCGTCAGATCAACGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGACCTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATgg < 1:249845/249‑1 (MQ=255) tGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGGAAAGCCCGGAACTACCGAAACGCAATGTAATTACTACT‑AAGCAAAATGGAACAcc > 1:489485/1‑251 (MQ=255) gaaagATTATCCACTTTAGCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACAcc < 2:336652/246‑1 (MQ=255) aataTTATCCACTTTATCCCTACCTGGAATACCGTCAGAGCACCGATGACCTGATGAATCAACCGGCTGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACTCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCTTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTa < 1:57882/183‑1 (MQ=255) aagATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAACCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCCGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACATCggg > 1:111195/3‑251 (MQ=255) cacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGCAGCGCCATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTga > 1:418969/3‑250 (MQ=255) tcacTACATGGAATACCGTCAGATCACCGATGACCTGATGAATCCACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCAGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGGCTGGCGTGGCTTGTTCACC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑TTGCCAAATGGAACACCGGACCGAGGGAAGAAGCCt > 2:399405/4‑251 (MQ=255) cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTg < 1:157987/251‑1 (MQ=255) cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATATCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTTGCCCGGGAAAGCCCGGAACTACCGAAGCGCACTGTAATTACTACT‑ATGCAAAATGGAACACCGGGCCGAGTGGAGAAACCTg > 1:108134/1‑251 (MQ=255) cTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGAGTGGCTTGTTAGCC‑TTTAGCCCGGGAAAGCCCGGGACTACAGAAGCGCAATGTAATTACTACT‑ATGAAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGc > 1:604992/1‑251 (MQ=255) ggAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAggg < 2:209593/250‑1 (MQ=255) aTACCGTCAGAGCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGa < 2:572484/251‑1 (MQ=255) gaCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGaa < 2:258653/250‑1 (MQ=255) gtcAGATCGCCTATGACCTGATGAATCAACCGGCGGTGACTGTCACTATCGTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTATCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACGCCGGGCAGAGTGAAGAAGGCTGGCAAGGTGGGAAagag < 2:271614/249‑1 (MQ=255) gtcAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAAACTGGCGTGGCTTGTTAACC‑TTTAGCCCGGAAAAACCCGGAACTACCGAAGCGCAATGTAATTACTATTTAT‑CCAAATGGAAAAACGGGCAGAGGGAAAAAGCCCGGCAAGGGGCGAAag > 1:438499/3‑249 (MQ=255) cAGATCACCTATGACCTGATGAATCAGCCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTTAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAAGGTAATTACTACT‑ATGCAAAATGTAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCt < 2:531208/251‑1 (MQ=255) atgacCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑CTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGAGGCGAAAGAGCTGTGGCTCAcc > 1:317356/1‑251 (MQ=255) gatgaATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAaga < 1:88055/251‑1 (MQ=255) gatgaATCAACCGGCGGCGACTGACACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCGTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGTGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCaa < 2:100247/249‑1 (MQ=255) atgaATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAAATGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGGACTACCGTAGCGCAATGTCATCAATAAT‑ATGCAAAATGGAACAACGGGCAGAGTGAAGACGGCTGGCAAGGGGGGAAAGAGCTGTGGCTAACCGGCAag > 1:628074/1‑249 (MQ=255) gaATCAACCGGCGGTGACTGTCACTAACTTTGTTCCCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGGGAAGAAGACTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGagcc > 1:170647/1‑251 (MQ=255) cAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGGAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAAAAACCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGagccagac > 1:130636/1‑249 (MQ=255) ggTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCGTACGCCt < 2:418969/251‑1 (MQ=255) gTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTTGCCCGGAAAAACCCGGAACTACCGAAGTGCAATGTAATTACTACT‑ATGCACAATGGAACACCGGGAACAGTGAAGAAACCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAACAGCCAGCCTAACGCCtg > 2:275829/1‑251 (MQ=255) gTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCGCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGTCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCAAAAGGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAAGCGGCGGGAGCCAGCCTAACGCCtg < 2:77935/251‑1 (MQ=255) | GCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACT‑ATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTG > NC_000913/4630742‑4631229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |