Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,945	C→T	R71R (CGC→CGT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,945	0	C	T	100.0%	272.1 / NA	85	R71R (CGC→CGT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/39);  total (46/39)

ATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAA  >  NC_000913/4630702‑4631190                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       aTTGATGTATTTACAATTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATc                                                                                                                                                                                                                                                  >  2:5453/1‑250 (MQ=255)    gATGTATTTACACTTAGAGGATCCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGGCAAATGGTTGTGGTCGAACAACTGATGCCTGGACTGAAAGCTTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCg                                                                                                                                                                                                                                              >  2:15678/1‑251 (MQ=255)       gTATTTACCCTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAGATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGAAAGATTATCCACTTTATCCCTACATGGAATACCGTCAGATCACCgatg                                                                                                                                                                                                                                           >  2:283955/1‑251 (MQ=255)       gTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCgatg                                                                                                                                                                                                                                           >  1:74062/1‑251 (MQ=255)             acacTTAGAGGATGCGCTGGGGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGGGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCGGGAATACCGTCAGAGCACCGATGACCtg                                                                                                                                                                                                                                      <  2:609623/250‑1 (MQ=255)                 ttAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga                                                                                                                                                                                                                                  >  1:441530/1‑250 (MQ=255)                       gATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAcc                                                                                                                                                                                                                           >  2:118196/1‑251 (MQ=255)                        aTGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcg                                                                                                                                                                                                                          <  1:378275/251‑1 (MQ=255)                         tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcgg                                                                                                                                                                                                                         >  1:522668/1‑251 (MQ=255)                           cgcTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcggcg                                                                                                                                                                                                                       >  1:74018/1‑251 (MQ=255)                           cgcTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATAAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGCACTGATGAATCAACcggcg                                                                                                                                                                                                                       >  1:78197/1‑251 (MQ=255)                               tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGCTGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTg                                                                                                                                                                                                                    >  1:77935/1‑250 (MQ=255)                               tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGt                                                                                                                                                                                                                     <  1:197490/249‑1 (MQ=255)                                gtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGa                                                                                                                                                                                                                   <  2:611886/250‑1 (MQ=255)                                   gAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACGTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcggc                                                                                                                                                                                                                        <  2:279197/242‑1 (MQ=255)                                   gAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGCGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcggc                                                                                                                                                                                                                        >  1:279197/1‑242 (MQ=255)                                     aaaaGCCAAACAAGTTCCCAGGCGGCGGTTGGCTGCCGGAGTCTGTCAGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGCTGCGCAGCGTAGCCGCTACGCGCAAATCAAGCAGGCCTGGGATAATCGCCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGCTGAATCAACcggcgtggcctgacc                                                                                                                                                                                                             >  2:75359/1‑241 (MQ=255)                                         gCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTa                                                                                                                                                                                                          >  1:284774/1‑250 (MQ=255)                                          ccAAACACGTTACCTGGCGAATGTTGGCAGCCGGTGTCAGTCTGCTGACGGTCAGCAGCGTGCCACGAGCCGCCTCACTGGATAAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCAGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGCTGACCTGATGAATCAACCGGCCGTGACTGTAACTaa                                                                                                                                                                                                         >  2:72015/1‑250 (MQ=255)                                          ccAAACAAGTTCACTGGCGACTGTTGGCTGCCCGTGTCTGTCTGCTGACGGTCAGCCGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAACTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGAct                                                                                                                                                                                                                 >  2:397596/1‑241 (MQ=255)                                          ccAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGGGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAct                                                                                                                                                                                                                 <  1:397596/242‑2 (MQ=255)                                                 aGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTc                                                                                                                                                                                                 <  1:320131/251‑1 (MQ=255)                                                     aCCTGGCGGCTGTTGGCTGCCGGTGGCTGTCGGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTcgcg                                                                                                                                                                                              <  2:449159/250‑1 (MQ=255)                                                        tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTACCTTTGTTCGCGCTa                                                                                                                                                                                           >  2:353574/1‑250 (MQ=255)                                                        tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTa                                                                                                                                                                                           >  1:171047/1‑250 (MQ=255)                                                            ggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAcccc                                                                                                                                                                                      <  1:238210/251‑1 (MQ=255)                                                                   ggCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtc                                                                                                                                                                               <  1:602573/251‑1 (MQ=255)                                                                        acGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGAGACTGTCACTAACTTTGTTCGCGCTAACAACACGCTTcccccc                                                                                                                                                                          >  2:8536/2‑251 (MQ=255)                                                                                tgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAATGTCACTAACTTTGTTCAAGCTAACCCCACGCTTCCTCCCGCTCgaac                                                                                                                                                                  >  1:598618/1‑248 (MQ=255)                                                                                 gtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc                                                                                                                                                                  <  2:497847/250‑1 (MQ=255)                                                                                      ctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGc                                                                                                                                                             >  1:41029/1‑250 (MQ=255)                                                                                                  agcGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGt                                                                                                                                                >  2:476874/1‑251 (MQ=255)                                                                                                   gcGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTc                                                                                                                                               <  1:353574/251‑1 (MQ=255)                                                                                                     gTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCaa                                                                                                                                             <  1:495373/251‑1 (MQ=255)                                                                                                           cgAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACt                                                                                                                                       >  2:521943/1‑251 (MQ=255)                                                                                                               ccGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgcg                                                                                                                                   <  2:550589/251‑1 (MQ=255)                                                                                                                     cACTGGACGAGCAGCTGAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCGGGACTGAAAGATTAGCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACGTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTTGCACGCTGCAATCTCGTATCGTGAATGAACTGGCGCGGCGt                                                                                                                             <  2:569550/251‑1 (MQ=255)                                                                                                                          gATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTACCTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCGCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGTGGCGTGAAGa                                                                                                                        <  2:403109/251‑1 (MQ=255)                                                                                                                           aTGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGAc                                                                                                                       >  1:521594/1‑251 (MQ=255)                                                                                                                                 agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGACTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAATGTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGt                                                                                                                 >  1:578106/1‑251 (MQ=255)                                                                                                                                   cgtagccggtaagcgaaaaTCAAGCAGGCATGGGATAATCGCCAAATGGATGTGGTCGAACAAATGAAGCCTGGACTGAAAGACTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGCTCGCGCAAACCCCACACTCCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGTACTGGCGCGGCGTGAAGACCGGCGTgg                                                                                                               >  2:89210/17‑251 (MQ=255)                                                                                                                                    gTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGc                                                                                                              >  1:279866/1‑251 (MQ=255)                                                                                                                                     tagccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGTTGTGGTCGCACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCt                                                                                                             >  2:543928/5‑251 (MQ=255)                                                                                                                                      agccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCtt                                                                                                            <  1:138440/248‑1 (MQ=255)                                                                                                                                          gTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGATTGTCCCTACCTTTGTTCGCGCTAACCCAAAGGTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGAACTGGCgcggactggaggaccacgtgggtgtttt                                                                                                        >  2:144351/1‑227 (MQ=255)                                                                                                                                            tACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCGGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCTGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGGGGCTTGTTAg                                                                                                       <  2:256425/250‑1 (MQ=255)                                                                                                                                               gcgcAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑tt                                                                                                  <  1:75359/251‑1 (MQ=255)                                                                                                                                                cgcAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCCCGGCGTGACGAATGGCGAGGCtttgttggcttt                                                                                                  >  2:596917/1‑241 (MQ=255)                                                                                                                                                       cAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCg                                                                                           <  1:585568/250‑1 (MQ=255)                                                                                                                                                                  gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCgg                                                                                <  1:278301/250‑1 (MQ=255)                                                                                                                                                                  gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCgg                                                                                <  1:520226/250‑1 (MQ=255)                                                                                                                                                                  gggATAATCGACAAATGGAAGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGCTGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCTTGTTTGCC‑CTTTGCCCCGAAAAACCCgg                                                                                >  2:371684/1‑250 (MQ=255)                                                                                                                                                                     aTAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc                                                                                                                                                       >  2:156315/1‑177 (MQ=255)                                                                                                                                                                     aTAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc                                                                                                                                                       <  1:156315/177‑1 (MQ=255)                                                                                                                                                                      tAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGCAGACTGGCGTGGCTTGTTTGCC‑TTTTGCCCGGGAAAGCCCGGAACtt                                                                           >  2:337716/1‑250 (MQ=255)                                                                                                                                                                            aCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGAGGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCTTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCACGGAACTTCCGAAg                                                                     <  2:291558/251‑1 (MQ=255)                                                                                                                                                                            aCAAATGGATGTGGTCGAACAAATGAGGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAg                                                                     <  1:42303/251‑1 (MQ=255)                                                                                                                                                                              aaaTGGATGTGGTCGAACAAATGATGCCTGGACGGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAAGGAACTGGCTCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAgcg                                                                   <  2:618271/251‑1 (MQ=255)                                                                                                                                                                                  ggATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAAt                                                               <  2:159175/251‑1 (MQ=255)                                                                                                                                                                                       tgGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTGGCCCTTTA‑CCCGGAAAAGCCCGGGACTACCGAAGCGCAATGGAAt                                                          >  2:188852/1‑251 (MQ=255)                                                                                                                                                                                        ggTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAAt                                                          <  1:118196/250‑1 (MQ=255)                                                                                                                                                                                        ggTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGGTAGGC‑TTTAGCCCGGGAAAACACGGAACTACCCAAGCGCAATGGAAtt                                                         >  1:218713/1‑251 (MQ=255)                                                                                                                                                                                         gtagAACAAATGAGGCCTGGACTGAAAGATTATCCCCTTTATCCCGTCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGTCTGTCACTAACTTTGTTCGCGCTAACCCCTCGCTTCCTCCCGCTCGCACGCTTCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAATC‑TTTAGCCCGGAAAAGCCCGGAACTGCCTAAGCGCAATGTAATta                                                        <  2:404373/248‑1 (MQ=255)                                                                                                                                                                                           caaaCAAATGATGCCTGGACTGAAAGATTAGCCACTTTATCCCTTCCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCTGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCGCTCACTCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGGGAAGACTGGCGTGGCTTGTTAGCC‑TTTATCCCGGAAAAGCCCGGAACTCCCAAAGCGGAGTCTAATtac                                                       <  2:33838/248‑1 (MQ=255)                                                                                                                                                                                           cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATtact                                                      <  1:171494/251‑1 (MQ=255)                                                                                                                                                                                           cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATtac                                                       <  1:185384/250‑1 (MQ=255)                                                                                                                                                                                             aaCAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACACCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTACC‑TTTAGCCCGGAAAAGCCCCGAACCAACCAAGCGCAAattaattaattc                                                    >  2:495698/1‑247 (MQ=255)                                                                                                                                                                                                 aaTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGACTACCGTCAGGTCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCAAGCTTCCTCCCGCTCGCCCGCTGCAATCACGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCC‑CTTAGTCCGGCAAAGCCCGGAACTACCGAAGCCCGATGAAAATACTACTATg                                                >  2:608584/1‑251 (MQ=255)                                                                                                                                                                                                 aaTGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGACTACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCCAAGCGCCATGTAATTCCTACTAtt                                                >  1:263240/1‑250 (MQ=255)                                                                                                                                                                                                  atgatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCCATGTAATTACTACTATGc                                               >  1:336652/1‑251 (MQ=255)                                                                                                                                                                                                     atgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCGTGTTAACC‑CTTAGCCCGGAAAAGCCCGGAACTACCCAAGCGCCATGGAATTACTCCTATGcaa                                             >  2:88055/1‑248 (MQ=255)                                                                                                                                                                                                         cTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAACCCGGAACTACCGCAGCGCCATGTAATTACTACTATGCAAAATgg                                        >  1:322262/1‑251 (MQ=255)                                                                                                                                                                                                           ggACTGAAAGATTATCCTCTTTATCCCTCCCTGGAATACCGTCAGATCAACGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGACCTACCGAAGCGCAATGTAATTACTACTATGCAAAATgg                                        <  1:249845/249‑1 (MQ=255)                                                                                                                                                                                                               tGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGGAAAGCCCGGAACTACCGAAACGCAATGTAATTACTACTAAGCAAAATGGAACAcc                                  >  1:489485/1‑251 (MQ=255)                                                                                                                                                                                                                gaaagATTATCCACTTTAGCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACAcc                                  <  2:336652/246‑1 (MQ=255)                                                                                                                                                                                                                  aataTTATCCACTTTATCCCTACCTGGAATACCGTCAGAGCACCGATGACCTGATGAATCAACCGGCTGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACTCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCTTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTa                                                                                                <  1:57882/183‑1 (MQ=255)                                                                                                                                                                                                                  aagATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAACCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCCGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACATCggg                               >  1:111195/3‑251 (MQ=255)                                                                                                                                                                                                                           cacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGCAGCGCCATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTga                       >  1:418969/3‑250 (MQ=255)                                                                                                                                                                                                                                  tcacTACATGGAATACCGTCAGATCACCGATGACCTGATGAATCCACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCAGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGGCTGGCGTGGCTTGTTCACC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTTTGCCAAATGGAACACCGGACCGAGGGAAGAAGCCt               >  2:399405/4‑251 (MQ=255)                                                                                                                                                                                                                                   cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTg              <  1:157987/251‑1 (MQ=255)                                                                                                                                                                                                                                   cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATATCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTTGCCCGGGAAAGCCCGGAACTACCGAAGCGCACTGTAATTACTACTATGCAAAATGGAACACCGGGCCGAGTGGAGAAACCTg              >  1:108134/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGAGTGGCTTGTTAGCC‑TTTAGCCCGGGAAAGCCCGGGACTACAGAAGCGCAATGTAATTACTACTATGAAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGc            >  1:604992/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAggg       <  2:209593/250‑1 (MQ=255)                                                                                                                                                                                                                                              aTACCGTCAGAGCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGa   <  2:572484/251‑1 (MQ=255)                                                                                                                                                                                                                                               gaCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGaa  <  2:258653/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCC‑TTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAA  >  NC_000913/4630702‑4631190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑