Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,553	A→G	intergenic (‑31/‑180)	ettA ← / → slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,553	0	A	G	100.0%	224.8 / NA	64	intergenic (‑31/‑180)	ettA/slt	energy‑dependent translational throttle protein EttA/soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/31);  total (33/31)

TTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGC  >  NC_000913/4630314‑4630798                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTc                                                                                                                                                                                                                                             <  1:188495/250‑1 (MQ=255)          ggcggCGGACCCGCTTCCCCTGCGTTGTTTTTCACAAGGCCCGCCATAATGCGCAGCAGGGGAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAg                                                                                                                                                                                                                                   <  1:131637/247‑1 (MQ=255)                 gACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGcc                                                                                                                                                                                                                            >  1:100247/1‑251 (MQ=255)                         cgacttgtgatacgttaaccAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATTTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTcc                                                                                                                                                                                                                     <  2:3616/231‑1 (MQ=255)                         cctcctccGATGTCTTAACAAATCCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACTCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGGTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTccc                                                                                                                                                                                                                    <  1:425866/244‑1 (MQ=255)                          ccccTTCGATGTCTTTACCACTGCCCGCCATAATGCGCAGCAGGGTAGCCTTACCCTCGCCGTACAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGATATGTTTTTCAAACTACGACGTTTCGGCGGAACACCTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTTATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTccc                                                                                                                                                                                                                    <  1:494109/248‑1 (MQ=255)                                 gATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAAt                                                                                                                                                                                                            >  1:609623/1‑251 (MQ=255)                                   tGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATcc                                                                                                                                                                                                          >  2:495373/1‑251 (MQ=255)                                       tttATCAATGACCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGCTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCCCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAgt                                                                                                                                                                                                      >  2:157987/1‑250 (MQ=255)                                        ttatccctGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAgtt                                                                                                                                                                                                     >  1:483778/8‑249 (MQ=255)                                           tCAATGCCCGCCATAATGCACAGCCGGGTAGCCTTAACCGCGCCGTTCAGACCCAGGACACCACTTTTTGCCCCAGGGAAGAAACTCAGCGAGATGTTTTTCAACATATGACGTTTCGGCGGAACAACTTTGACGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGCAGTGTAGCCTTTTTCCCCGCCTACTCCCAgttggt                                                                                                                                                                                                  >  2:311568/1‑246 (MQ=255)                                                ccccGCCAGAATGCGCATCAGGGTAGACTTACCCGCGCCGTTCAGGCCCAGGACACCAATTTTGGCCCCATGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACGTTGCCGACACGATTCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTTGATGTGTATCGTGAGGATGAGTTTTCAAAGGCGAAGTTTAGCCTTGGTCCCCGCCTAATCCCAgttggccgatc                                                                                                                                                                                             <  2:623969/250‑11 (MQ=255)                                                  ccGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGCAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCAc                                                                                                                                                                                           >  1:33838/1‑251 (MQ=255)                                                                      gtagACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGCTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACATTCGCAgtaaaagtaaaa                                                                                                                                                                        >  1:256425/4‑250 (MQ=255)                                                                         gACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGGGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGTTGCATGGTATAACCGAATTGAGCCCCGTTGGACTTCGCCTCTATGTTTATCGTGATGATGGGTTTTCAAAGGCGAGGTTTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAgt                                                                                                                                                                    >  2:340562/1‑251 (MQ=255)                                                                             tACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAgtgt                                                                                                                                                                  <  1:60654/249‑1 (MQ=255)                                                                              aCCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCg                                                                                                                                                               >  1:70702/1‑251 (MQ=255)                                                                                   cgcCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTAAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTACCCGCCTAATCCCAGTTGGTCGATCACCTTAGCAGTAAAAGTAAAAAAGTGTCCGTAACg                                                                                                                                                          >  1:403109/1‑251 (MQ=255)                                                                                     ccgttCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAACGTAAAAAAGTGTCCGTAACGTg                                                                                                                                                        >  1:618271/4‑251 (MQ=255)                                                                                      cgttCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATGGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGGGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGGCCGTAACGTgg                                                                                                                                                       <  1:96131/249‑1 (MQ=255)                                                                                      cgttCAGACCCAGGACACCAAGTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTgg                                                                                                                                                       <  1:625231/249‑1 (MQ=255)                                                                                              cccAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGATTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCAACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATACCAGTTGGTCGATCACCTTCGCAGTAAAAGTAACAAAGTGTCCGTAACGTGGCGTAAACg                                                                                                                                               >  1:50395/1‑251 (MQ=255)                                                                                                    acacCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATg                                                                                                                                         >  1:550589/1‑251 (MQ=255)                                                                                                           tttttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTc                                                                                                                                                                                                                      >  2:325400/1‑167 (MQ=255)                                                                                                           tttttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTc                                                                                                                                                                                                                      <  1:325400/167‑1 (MQ=255)                                                                                                            ttttGCCCCAGGGAAGAAACTCAGAGAGATGTTTT‑CAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAATAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTa                                                                                                                                 <  2:524479/250‑1 (MQ=255)                                                                                                             tttGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGtt                                                                                                                                  >  1:449159/1‑249 (MQ=255)                                                                                                                  cccAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGGAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATa                                                                                                                            >  1:158898/1‑250 (MQ=255)                                                                                                                   ccAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATaaa                                                                                                                          <  1:113107/251‑1 (MQ=255)                                                                                                                      gggAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCt                                                                                                                       >  2:485684/1‑251 (MQ=255)                                                                                                                              aCTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTAcgcg                                                                                                               >  1:335720/1‑251 (MQ=255)                                                                                                                                 cAGAGAGATGTTTTTCAAAATATGACGTTGCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCa                                                                                                            <  2:483778/251‑1 (MQ=255)                                                                                                                                    agagaTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCGATTACGCGGCATGa                                                                                                         <  1:161929/251‑1 (MQ=255)                                                                                                                                     gagaTGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGAc                                                                                                        <  1:572452/251‑1 (MQ=255)                                                                                                                                          gTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGc                                                                                                   <  1:340562/251‑1 (MQ=255)                                                                                                                                              ttCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCAtt                                                                                                <  1:505491/250‑1 (MQ=255)                                                                                                                                                cAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGa                                                                                              <  1:124364/250‑1 (MQ=255)                                                                                                                                                  aaaTATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGt                                                                                           >  2:530987/1‑251 (MQ=255)                                                                                                                                                             ttCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCCCGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTACAACAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACtt                                                                                 >  1:622361/1‑250 (MQ=255)                                                                                                                                                                  cggAACAACTTTGCCGACACGATGCATGGTATACACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCACAGTTGGTCGATCACCTTCGAAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAgg                                                                            >  1:531239/1‑250 (MQ=255)                                                                                                                                                                   ggAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCGCTATGTTTATCGTGATGATGAGTTTTCAAAGTCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTTCATTGATGTATTTACTCTTATAGGAt                                                                          <  2:598401/251‑1 (MQ=255)                                                                                                                                                                    gAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATg                                                                         <  2:23611/251‑1 (MQ=255)                                                                                                                                                                     aacaacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAATTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgc                                                                        <  1:55064/251‑1 (MQ=255)                                                                                                                                                                     aacaacTTTGCCGACACGATGCATGGTATAAACGAATTGAGACACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgc                                                                        >  2:602573/1‑251 (MQ=255)                                                                                                                                                                      acaacGTTGCCGACACGATGCATGGTTTAAACGAATTGAGCCCCGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTTTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgcg                                                                       <  1:588344/251‑1 (MQ=255)                                                                                                                                                                       caacTTTGCCGACACGATGCATGGTATAAACGCATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgcgc                                                                      >  2:238210/1‑251 (MQ=255)                                                                                                                                                                       caacTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATgcgc                                                                      >  2:57794/1‑251 (MQ=255)                                                                                                                                                                             tGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTgg                                                                >  1:188433/1‑251 (MQ=255)                                                                                                                                                                              gCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTgg                                                                >  1:611886/1‑250 (MQ=255)                                                                                                                                                                              gCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGa                                                               <  1:42/251‑1 (MQ=255)                                                                                                                                                                                cGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaaa                                                             <  1:57794/251‑1 (MQ=255)                                                                                                                                                                                cGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGaa                                                              >  2:138440/1‑250 (MQ=255)                                                                                                                                                                                         gCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGGTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTGAGAGGATGCGCTTGTGGAAAAAGCCaaa                                                     <  2:378294/250‑1 (MQ=255)                                                                                                                                                                                          aaTGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGCCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACa                                                   <  2:232457/250‑1 (MQ=255)                                                                                                                                                                                             ggTATAAACGAATTGAGCCCCGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTGTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGTCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACGTAGAGAATGCGCTTGGGGAAAAAGCCAAACAAg                                                 <  2:321023/250‑1 (MQ=255)                                                                                                                                                                                             ggTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGGGATGATGAGTTTGCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGt                                                <  1:73934/251‑1 (MQ=255)                                                                                                                                                                                                           gAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGt                                  <  2:488712/251‑1 (MQ=255)                                                                                                                                                                                                                  gTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGATGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGc                           <  2:70702/251‑1 (MQ=255)                                                                                                                                                                                                                     ggACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCgg                        >  2:197490/1‑251 (MQ=255)                                                                                                                                                                                                                     ggACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAACCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCgg                        >  2:320131/1‑251 (MQ=255)                                                                                                                                                                                                                        cTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGgtgt                     >  2:25303/1‑251 (MQ=255)                                                                                                                                                                                                                             ccTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAACAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGAGGCTGTTGGCTGCCGGtgtctgtc                >  1:501313/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTcagc  <  2:559731/251‑1 (MQ=255)                                                                                                                                                                                                                                           gTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCACAGTTGGTCGATCACCTTCGCAGTAAACGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGAAGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTaagc  >  1:569550/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      TTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTAACGTGGCGTAAACGGCAATGACTGGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGC  >  NC_000913/4630314‑4630798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑