Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,629,896 T→C E209E (GAA→GAG)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,8960TC99.0% 338.1 / NA 104E209E (GAA→GAG) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (49/54);  minor base A (1/0);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 104 total aligned reads displayed.

GACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGT‑ACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTT  >  NC_000913/4629652‑4630137
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
gACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGa                                                                                                                                                                                                                                               >  2:457603/1‑249 (MQ=255)
            cTTTCGACTGCCGGACTTTAGTTCCCAGCCGTTACCCAT‑CCAGCTATTTCCCGCTCGCCTTACGACCCGACGCTTCCTGTGAAGCTTCCTGCGCCAGGCGATGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCACGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGATATCGCCACCACGGTGCCCTCgcagccgagcggga                                                                                                                                                                                                                                   >  2:283600/1‑236 (MQ=255)
             tttCGACTGGCGGCCTTTAGTTCCCTGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGCGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGc                                                                                                                                                                                                                                >  2:617601/1‑251 (MQ=255)
              ttCGACTGGCGGCCTTTAGTTCCCTGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCACGTTCGAGGTTCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCg                                                                                                                                                                                                                               >  1:92909/1‑251 (MQ=255)
                  aCTGGCGGCCTTTAGTTCCCTGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTcc                                                                                                                                                                                                                           <  2:503315/251‑1 (MQ=255)
                     ggcGGCCTTTAGTTCCCTGACGT‑TCCCACTCCAGCCCTTTCTCGATCGACTTACGTCGCGCCGCTTCTTGTGAAGTTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCGTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTcca                                                                                                                                                                                                                          <  2:344944/247‑1 (MQ=255)
                      gcGGCCTTTAGTTCCCGGACGT‑ACCCACCCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGATGAAGTAACGGTCGTGGGTAAGCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTccagcc                                                                                                                                                                                                                       <  1:226507/250‑1 (MQ=255)
                       cGGCCTTTAGTTCCCTGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTccagcc                                                                                                                                                                                                                       >  1:509855/1‑250 (MQ=255)
                                gttccctGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGa                                                                                                                                                                                                             <  2:611869/245‑1 (MQ=255)
                                  tccctGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCACGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGCAATACCTTCACCGCGGTCAAGGTCGAGGATCCAGCCTGCAACGTTATCGATGAAGTAACGGTCGTGGGTAATCGCCATCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGAt                                                                                                                                                                                                            <  1:627681/246‑1 (MQ=255)
                                    cctGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGc                                                                                                                                                                                                                   <  1:347806/239‑1 (MQ=255)
                                    cctGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGc                                                                                                                                                                                                                   >  2:347806/3‑241 (MQ=255)
                                    cctGACGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCg                                                                                                                                                                                                         >  1:454547/3‑251 (MQ=255)
                                         cGT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATc                                                                                                                                                                                                    >  1:223719/1‑251 (MQ=255)
                                          gT‑ACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATcc                                                                                                                                                                                                   >  1:279914/1‑251 (MQ=255)
                                                  ctCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGCAGCTTCCCGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACAGCGGTCAAGTTCGAGGATCCAGCCTGCACCGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGCAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAggtggt                                                                                                                                                                                            >  1:359391/2‑251 (MQ=255)
                                                      aGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCAACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCCCGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGtggttggt                                                                                                                                                                                        >  2:507413/1‑251 (MQ=255)
                                                            ttCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGtt                                                                                                                                                                                   <  2:148982/250‑1 (MQ=255)
                                                            ttCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGtt                                                                                                                                                                                   <  1:595841/250‑1 (MQ=255)
                                                            ttCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGtt                                                                                                                                                                                   <  1:161876/250‑1 (MQ=255)
                                                              cgcgaTCGGCTTACGACGCGCCGCTTCTTGTGAAGCGTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATGCGGCATCCAGGTGTTTGGTCGGTtcg                                                                                                                                                                                 <  2:440325/246‑1 (MQ=255)
                                                              cccgaTCGACCTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATGCCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTTGTTGGTCGGTtcg                                                                                                                                                                                 <  2:223719/246‑1 (MQ=255)
                                                                cgaTCGACTTCCGACGCGCCGCTTCTGGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTTGTTGGTCGGTtcgtc                                                                                                                                                                               <  2:405585/248‑1 (MQ=255)
                                                                cgaTCGACTTACGACGCGCCGCGTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTtcgtcg                                                                                                                                                                              <  1:558468/249‑1 (MQ=255)
                                                                 gagcGACTTACGACGCGCCGCTTATTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAAGCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGa                                                                                                                                                                             <  2:102155/248‑1 (MQ=255)
                                                                 gaTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTtcgtcg                                                                                                                                                                              >  1:14627/2‑250 (MQ=255)
                                                                 gaTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCACACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTtcgtcg                                                                                                                                                                              >  1:540860/2‑250 (MQ=255)
                                                                  aTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTtcgtcg                                                                                                                                                                              >  1:323231/1‑249 (MQ=255)
                                                                    cGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCCCCGCGGTCAAGTGCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGGGGTTGGTCGGTACGGCGagc                                                                                                                                                                           <  1:194175/250‑1 (MQ=255)
                                                                    cGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGagc                                                                                                                                                                           <  1:215711/250‑1 (MQ=255)
                                                                      aCTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGagcagc                                                                                                                                                                        >  2:483612/1‑251 (MQ=255)
                                                                        ttACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGagcagc                                                                                                                                                                        >  1:542388/1‑249 (MQ=255)
                                                                          acgacgCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGagcagcagc                                                                                                                                                                     >  2:171855/1‑250 (MQ=255)
                                                                              cgcgCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTc                                                                                                                                                                >  2:111215/1‑251 (MQ=255)
                                                                               gcgcCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATGCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCt                                                                                                                                                               <  1:372195/251‑1 (MQ=255)
                                                                                  ccgcCTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGGTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGGGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGt                                                                                                                                                            <  2:109802/251‑1 (MQ=255)
                                                                                    gcTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGttt                                                                                                                                                          >  1:602019/1‑251 (MQ=255)
                                                                                     cttcttGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGACAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGGCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCAAGGAGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGtttt                                                                                                                                                         >  2:539317/1‑251 (MQ=255)
                                                                                       tcttGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGttttt                                                                                                                                                        <  2:162954/250‑1 (MQ=255)
                                                                                          tgtgAAGATTCCTGCGCCAGGCGCTGATCTTTCTGCGCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCATCGTTATCGAGGAAGTAACGGTCGTGGGTAAGCGCCACCACGGTGCCCTCGAGGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCAGCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCca                                                                                                                                                     <  1:278482/250‑1 (MQ=255)
                                                                                          tgggAAGCTTCCTGCGCCAGGCGCTGATCTTTCTTCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTcc                                                                                                                                                      <  2:2572/246‑1 (MQ=255)
                                                                                           gtgAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGCATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGGAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGAGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCCGCATGTCTGGTTTTTCcagc                                                                                                                                                   >  1:93264/1‑251 (MQ=255)
                                                                                            tgAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGGTTTTCcagc                                                                                                                                                   >  1:3616/1‑250 (MQ=255)
                                                                                                   tCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAggcg                                                                                                                                           >  1:461611/1‑251 (MQ=255)
                                                                                                    ccTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAggcgg                                                                                                                                          <  1:9261/251‑1 (MQ=255)
                                                                                                    ccTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATAGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGCTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTTGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAggcgg                                                                                                                                          >  1:381643/1‑251 (MQ=255)
                                                                                                      tGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGCGGAGTAGTTGCCTTCCCACGGCATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAggcggcc                                                                                                                                        >  2:121089/1‑250 (MQ=255)
                                                                                                       gcgcCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGcac                                                                                                                                       <  2:349095/251‑1 (MQ=255)
                                                                                                        cgcCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGcaca                                                                                                                                      <  1:111215/251‑1 (MQ=255)
                                                                                                            aGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGc                                                                                                                                  >  2:455047/1‑251 (MQ=255)
                                                                                                            aGGCGCTGATCTTTCTGCTCCAGACAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGCTCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCCGGCCACGGATTCGGCCTCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACACCGc                                                                                                                                  >  2:381555/1‑251 (MQ=255)
                                                                                                                gcTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTAcg                                                                                                                              >  1:72931/1‑251 (MQ=255)
                                                                                                                gcTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCGAcg                                                                                                                              >  2:505387/1‑251 (MQ=255)
                                                                                                                acTGATCTTTCTGCCCCAGCCAGGAGGAGTAGGTGCCTTCCCACGGAATTCCTTCCCCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAGGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGGTTTTCCAGCAGCAGGCGGCACAACGCTAcg                                                                                                                              <  1:203290/250‑1 (MQ=255)
                                                                                                                       tttCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGCTTTTCCAGCAGCAGGCGGCACAACGCTACGCgacgac                                                                                                                       >  2:416367/1‑251 (MQ=255)
                                                                                                                        ttCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCATCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGGTTTTCCATCAGCAGGCGGCACAACGCTACGcgac                                                                                                                          <  2:406679/247‑1 (MQ=255)
                                                                                                                        ttCTGCGCCAGCCAGGAGGAGTAGGTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTGCCAGCAGCAGGCGGCACAACGCTACGcgactac                                                                                                                       <  2:164980/250‑4 (MQ=255)
                                                                                                                        gtcggCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGcgac                                                                                                                          <  2:382588/243‑1 (MQ=255)
                                                                                                                            gCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTca                                                                                                                  <  1:502635/251‑1 (MQ=255)
                                                                                                                                cagacagGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCAAGCCTGCAACGATATCGCGGAAGTAACGGTCGTGGGTCATCGCCACCACGGTGCCCTCGCAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGAGTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGAAGCAGGCGGCACCCCGCAACGCGACGACGCTcacccc                                                                                                              >  2:82280/5‑249 (MQ=255)
                                                                                                                                            gtagtTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTGATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGTCGGCACAACGCTACGCGACGACTCTCACCACCGGAGAGGTTAg                                                                                                  <  2:359391/251‑1 (MQ=255)
                                                                                                                                              agtTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGCAGTCGTGCCGGAAGCGTTCCAGCCAGGCCACGGCTTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCg                                                                                                >  1:619410/1‑251 (MQ=255)
                                                                                                                                              agtTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCg                                                                                                <  2:395772/251‑1 (MQ=255)
                                                                                                                                                   ccTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGAtttt                                                                                           <  2:176578/251‑1 (MQ=255)
                                                                                                                                                       cccACGGAATATCTGCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTGACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGAGTCGTCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTTGTTGTTCCAGGAGCAGGCGGCACAACGCTACGCGGCGACGCACACCACCGGTGAGGTTAGCGATTTTcgcg                                                                                       <  2:223191/251‑1 (MQ=255)
                                                                                                                                                            ggAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCa                                                                                  <  2:454547/251‑1 (MQ=255)
                                                                                                                                                             gAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGCGGTTAGCGATTTTCGCGTccc                                                                                   >  2:602776/1‑249 (MQ=255)
                                                                                                                                                             gAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTccc                                                                                   >  1:502109/1‑249 (MQ=255)
                                                                                                                                                             gAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTAGCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGGCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCGCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAg                                                                                 <  2:623962/251‑1 (MQ=255)
                                                                                                                                                               aTACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTCTCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGGGCAGGAAGCGTTCCCGCCAGGCCACGGATTCGGCATCCCGGTGGTTGGGCGGGTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCATCAGGCGTCACAACGCTACGCGCCGACCCCCACCCCCGGAGTGGTTGGCGCTTTTCGCGTCCCacac                                                                               >  2:5503/1‑248 (MQ=255)
                                                                                                                                                                tACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTcc                                                                              >  2:172845/1‑251 (MQ=255)
                                                                                                                                                                  ccTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCgg                                                                            >  1:138392/1‑251 (MQ=255)
                                                                                                                                                                          gcgGTCAAGTGCGAGGATCCAGCCTTCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGGCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTGCTCTTCCCAGTCCGTCAGTCGTa                                                                    <  2:388627/251‑1 (MQ=255)
                                                                                                                                                                           cgGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGGTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACTCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAg                                                                   <  1:578732/251‑1 (MQ=255)
                                                                                                                                                                               cAAGTTCGCGGAACCAGCCTGCAACGTTATCGAGGAAGTAACGGTAGTGGGTAATCGCCACCACGGGGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGGGGTTGGACGGTTCGTCGGGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGATAGCGATTTGCGCGTCACAGGCCGGCAGACGTAgctc                                                                >  2:588344/1‑248 (MQ=255)
                                                                                                                                                                                      gAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTGTCCAGCAGCAGGCGGCACAACGCTACGCTACTACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGGCCGGCAGACGTAGCGCATccgccg                                                        <  1:283600/251‑1 (MQ=255)
                                                                                                                                                                                      gAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTAGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCAAGGATTCGGCATCCAGGGGGTTGGACGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCCGCAGAAGGCGGAACAACGCTACGCGCCGACGCTCACCACCGGAGTGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATccgccg                                                        >  1:266568/1‑251 (MQ=255)
                                                                                                                                                                                            ccAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCt                                                  <  2:468212/251‑1 (MQ=255)
                                                                                                                                                                                               gCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCa                                               <  1:121089/251‑1 (MQ=255)
                                                                                                                                                                                                 cTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGc                                             >  2:572792/1‑251 (MQ=255)
                                                                                                                                                                                                   gCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTg                                           <  1:540562/251‑1 (MQ=255)
                                                                                                                                                                                                    caacttttgcGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGCCGGGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCAGCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCGGGGTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGGCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGt                                          <  2:453542/242‑1 (MQ=255)
                                                                                                                                                                                                           aTCGAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCa                                   <  1:583948/251‑1 (MQ=255)
                                                                                                                                                                                                              gAGGAAGTAACGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAggt                                <  1:457603/251‑3 (MQ=255)
                                                                                                                                                                                                                  aaGTAGCGGTCGTGGGGAAGTGACACCACGGTGCCCTCGAAGGCGTGCAGGAGGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTGGGGCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCCCCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGTCGTAGCGCATCCTCCGCACGCTCCAGCTGTACGTTCAGGTTATg                            <  2:199135/251‑1 (MQ=255)
                                                                                                                                                                                                                      aaCGGTCGTGGGTAATCGCCACCCCGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATGCGGCAGCCAGGTGGTTGGTCGATTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACCACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGGCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTTTACGTTCAGGTTATGACcg                        <  2:134379/251‑1 (MQ=255)
                                                                                                                                                                                                                      aaCGGTCGTGGGTAATCGCCACCACGGTGCCATCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACcg                        >  2:409969/1‑251 (MQ=255)
                                                                                                                                                                                                                        cGGTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGGCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACcgtc                      <  2:247658/251‑1 (MQ=255)
                                                                                                                                                                                                                         ggTCGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACcgtcg                     <  2:15539/251‑1 (MQ=255)
                                                                                                                                                                                                                          gtctttggTAATCGCCACCACGGTGCCCCCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCTGGTGGTTGGTCGGTTCGTCGAGCAGCATCATGTCTGGTTTTGCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCGCACCACCGGAGAGGTTAGCGATTTTCGCGGCCCAGTCCGGCAGTCGTAGCGCATCCGCCGCACTCTCCAGCTGTACGTTCAGGGTATGACcgtcgt                    <  2:463489/245‑1 (MQ=255)
                                                                                                                                                                                                                            cGTGGGTAATCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTg                   <  1:387522/250‑1 (MQ=255)
                                                                                                                                                                                                                                   aaTCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGa            <  1:53022/250‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAAt          >  1:263566/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cGCCACCACGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATg         >  1:69665/1‑250 (MQ=255)
                                                                                                                                                                                                                                        cccccaCGGTGCCCTCGAAGTCGGGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGCCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTCGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCTCACGCTCCAGCTGTGCTTTCAGGTTATGACCGTCGTGAGCCTGAATGAtc      <  2:454852/246‑1 (MQ=255)
                                                                                                                                                                                                                                         caccacgttGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGATACGCGACGCCGATCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCCGGTTATGACCGTCGTGAGCCTGactgctct     >  2:27854/9‑244 (MQ=255)
                                                                                                                                                                                                                                         caccaCGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGAtct     >  1:517857/5‑251 (MQ=255)
                                                                                                                                                                                                                                           ccaCGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATctct   >  2:286501/3‑251 (MQ=255)
                                                                                                                                                                                                                                            caCGGTGCCCTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCtt  <  2:14627/250‑1 (MQ=255)
                                                                                                                                                                                                                                            caCGGTGACCTCGAAGTCGTGCAGGAAGCGTTCCAGACAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCCTCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCtt  >  2:24686/2‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
GACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGT‑ACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGGGTAATCGCCACAACGGTGCCTTCGAAGTCGTGCAGGAAGCGTTCCAGCCAGGCCACGGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTT  >  NC_000913/4629652‑4630137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑