| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,206 | A→T | A290A (GCA→GCT) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,206 | 0 | A | T | 97.9% | 278.9 / NA | 94 | A290A (GCA→GCT) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (46/46); total (47/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
ACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑ACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACG > NC_000913/962‑1451 | actccgcggcGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGGGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGGGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCGAAAGTTCTTCCCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAAATCCCTTGCCTGATTACAAATACCGGGAATCCTCATGC‑TCCAGGt > 1:533448/9‑251 (MQ=255) ggcGGTGCTGGCTGCCTGTTTACGCGCCGGTTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGGGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGGAGCGCTGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGCTCACTTGCCTGATTAACAATACCGGAAATCCTCATGC‑TCCAGGTACGCTcc > 2:352360/2‑250 (MQ=255) tgctgcCTGCCTGATTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGACTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGACGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACAATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTCAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGt > 2:298211/1‑251 (MQ=255) ggctgCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCa < 1:119341/251‑1 (MQ=255) cTGTTTAAGAGCCGAATGGTGCGGGATTCGGATGGGCGTTGACGGGGTCTATACCAGCGACCAGCGTCAGGTGCCCGATGCGCGGTTGTTGGCGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTAATTCGGCGCTAAGGTTCTTCACCCCCGCACCATTAACCCC‑ATCGCCCAGTTCAAGATCCCTTGCATGATTAAAAATACCGAACAACCACAAGC‑TCCAGGTAACCTCATTGGTGCAAGCCGtg > 2:359262/1‑251 (MQ=255) gTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGtgat < 1:581830/251‑1 (MQ=255) ttACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCGATTCCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑ACCAGGTACGCTCATGGGTGCCAGCCGtgatg < 2:84610/250‑1 (MQ=255) aCGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAg < 1:117107/251‑1 (MQ=255) cgcgCCGATTGTTGCGAGATTTGGCCGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGCAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCATTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAgc > 2:608666/1‑250 (MQ=255) gcgcCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGCTGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCCCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGAc < 1:627216/251‑1 (MQ=255) cgcCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGAc > 1:478149/1‑251 (MQ=255) cGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAAt > 1:34342/1‑251 (MQ=255) tgttgCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTAcc > 2:218988/1‑251 (MQ=255) gttgCGAGATTTGGACTGACTTTTACGGGGTTTATACCGGCGACCCGCTTCAGGTGCCCGATGCGAGGTTGTTGAAGGCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACGTCGGCGCGAAAGCTCTTCCCCCCCGCACTATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTAATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCAGATTGTTGCCATCCGGGATGAAGACCAATTAc < 2:64597/250‑1 (MQ=255) cGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACAGGTc > 1:371223/1‑250 (MQ=255) agaTTTGGACGGACGTTGACGGGGTCTATACCTGCGCCCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCGTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATGGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa < 2:215571/251‑1 (MQ=255) aTTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAggg > 2:306809/1‑251 (MQ=255) aTTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAggg > 1:68146/1‑251 (MQ=255) tttGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGCTGAAGAAGAATTACCGGTCAAGGGc > 2:529252/1‑251 (MQ=255) tGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCCGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACACCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCAt > 2:253078/1‑251 (MQ=255) ggacgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCa < 1:462142/251‑1 (MQ=255) ggacgTTGACGGGGTCTATACCTGCGACCCGCGGCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTGGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAATACGAAGTACCGGTCAAGGGCATTTCCa < 2:420413/251‑1 (MQ=255) gacgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCTCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCaa < 1:272730/251‑1 (MQ=255) gacgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGACATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTcc > 2:56547/1‑250 (MQ=255) gacgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCa < 1:114297/251‑1 (MQ=255) acgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAAt < 1:198302/251‑1 (MQ=255) acgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGAGGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCAACATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAATC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAAt < 2:172222/251‑1 (MQ=255) cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCATATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATc < 1:616003/251‑1 (MQ=255) gACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTg < 1:8622/250‑1 (MQ=255) cGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGTAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCa < 1:97432/251‑1 (MQ=255) gACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCGACCAGGAAGCGATGGAGCTGTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAg < 2:198867/251‑1 (MQ=255) aCCCGCGTCAGGTGCCCGATGCGAGTTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAgt < 2:25655/251‑2 (MQ=255) cgTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTc < 1:345777/251‑1 (MQ=255) gTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCCCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGGCCGCTCATTGTTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTcc > 2:113115/1‑250 (MQ=255) gCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTccggcccg > 1:415608/1‑244 (MQ=255) aTGCTAGGTTGTTGAAGTCGATGTTCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGGTCTTCTCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATTCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCTGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATg < 2:12359/251‑1 (MQ=255) gttgttGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGAGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAggg > 1:273300/1‑250 (MQ=255) tgttgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGAt < 1:227933/250‑1 (MQ=255) gttgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATg < 1:69372/250‑1 (MQ=255) gAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATACCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGtt > 1:100579/1‑250 (MQ=255) gATGTCCTACCAGGAAGCGATGGCGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATgg > 1:149596/1‑251 (MQ=255) tACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGgcgcg > 2:243403/1‑251 (MQ=255) aCCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGgcgcgc > 2:121099/1‑251 (MQ=255) aGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGtg > 1:519283/1‑250 (MQ=255) aGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTg > 1:231809/1‑251 (MQ=255) attgAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTGCCAGATCCCTTGCCTGATTAAAAAGTCCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGTTGCCAGCCGTTATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAAGGTTCTGCGTTTCCTGCCCGGGTATTAAAGGGATGGGTGGCATGGCGgcgcgctggtttgcagc < 2:412174/248‑12 (MQ=255) aTGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCCTTCACCCC‑ATCGCCCAGTTCCAGATCACTTGACTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACACTCATTGGTGCCAGCCGTGATGAAGACGACTTACCGGCCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGGGTGTTTGCAGc > 1:296005/1‑251 (MQ=255) tGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TACAGGTACGATCATTGGTGCCAGCCGTGATGAAGACGAATTACAGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCg > 1:75706/1‑251 (MQ=255) ggAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATCACACCC‑ATCGCCCAGTTCCAAACCCCTTGCCTGATTAAAAATAACGCCAATCCTCAAGCTACC‑GGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAACGGCATTTCCAATCTGAAATATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGTCGCGCGGCTTTGCAGCg > 1:581603/1‑250 (MQ=255) tttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGgtgta > 2:483405/1‑249 (MQ=255) tttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTc > 2:357042/1‑250 (MQ=255) ttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCa < 1:357042/250‑1 (MQ=255) cTTCGGCGCTAAAGTTATTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGACAGGGATGGTTGGCATGGCGGCGCGGGTGTTTGCAGCGATGTCACGCGccc > 1:515480/1‑251 (MQ=255) tCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTTCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGccc < 1:582031/250‑1 (MQ=255) gcgcTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCGTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTTGTGCCAGCCGTGATCAAGTCGAATTACCGGTCAAGGGCATTTCCTATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGTTGATGAAATGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGt < 1:402833/249‑1 (MQ=255) gcTAAAGTTCTTCCCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTc < 1:591227/251‑1 (MQ=255) gTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCggggg > 1:172979/1‑247 (MQ=255) tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATGCCGGAAATCCTCAAGC‑TCCAGTTACGCTCATTGTTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGGATCCGGCCCGGGGATGAGAGGGATGGTTGGCATGTCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCt < 1:359262/249‑1 (MQ=255) tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGTTTGCAGCGCAGTCACAAGCCCGTATTTCCG‑GGGTGCttg > 1:53861/1‑249 (MQ=255) tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTAAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCt < 1:352360/249‑1 (MQ=255) cACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGCTTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTGTGCGGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGAt > 2:189294/1‑251 (MQ=255) ccccccGCACCATTACCCCC‑ATCGCCCAGTGCCAGATCCCGTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATGACCGGTCAAGGGCATTTCCAATCTGAATAATATGTCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGTTTGTCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGAtt < 2:123938/250‑1 (MQ=255) cGCACCATTACCCCC‑ATCGCCCCGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCG‑TGGTGCTGATTACGc > 1:161638/1‑250 (MQ=255) aCCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGGTTACGCAatc > 1:384469/1‑251 (MQ=255) aCCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatc < 1:243403/251‑1 (MQ=255) ccATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGTTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatca < 1:23007/251‑1 (MQ=255) cATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatcat > 1:65395/1‑251 (MQ=255) aTTACCCCC‑AGCGCCCAGTTCCAGATCCATTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatcatc < 1:608666/251‑1 (MQ=255) tACCCCC‑CTCGCCCAGTTCCAGATCCCTTGCATGATTACAAATACCGGAAATCCTCAAGC‑TCCAGGTCCGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGGTGTCCCGCGCCCGCATTTCCG‑TGGTGCTGATTTCGCaagc > 2:102503/1‑244 (MQ=255) tACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatc < 1:102503/246‑1 (MQ=255) tcccacagcGCCCAGTTCCATGTCCCTCGCCTGACTACAAAGTCCGGACAACCTCAAGC‑TCCAGCTGCGCTCTTTGGTGCCAGCCGTGATTCAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATTATATAGGAATGTACAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCTGAGCGCGTGTTTGCAGCGATGTGACGCGCCCGTATGTCCG‑TGGTGCGGATTCCGCAATCATCtt < 2:337812/242‑1 (MQ=255) cccccacagCCCAGTTCCAGATCCCTTGCCGGATTAAAAATCCCTGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTTCCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGGTTGGTATGGCGGCGCACGGGGTTGCAGCGATGTCTCGCGCCCGTTTTTGCGATGG‑GCTGATTACGctgtcagcttcc < 2:603549/243‑12 (MQ=255) ccAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCGTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCTGTCAAGGTCATTTCCAATCTGAATAATATGGCAATGTGCAGCTTTTCCGGCCCGGGGATTAAAGGGATGGTAGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGGATTCATCTTCCTAATACatta < 2:35527/251‑4 (MQ=255) ccAGTACCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCGCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTGCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCTCGCGCCCGTATATCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAgta < 2:60219/251‑3 (MQ=255) cAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAACACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGttt > 2:414465/1‑251 (MQ=255) aGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCCGCGCTGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTc > 1:38696/1‑251 (MQ=255) gATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCt < 2:161638/251‑1 (MQ=255) ttGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTc > 1:561776/1‑251 (MQ=255) gCCTGATTAAAAATACCGGAACTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTccg > 2:110636/1‑250 (MQ=255) cTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGTCACGCGCCCGGATTTCCG‑TGGTGCTGGTTACGCAATCATCTTCCGCATACAGTATCAGTTTCTGCGTTccgc > 2:472822/1‑248 (MQ=255) cTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGGTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTccgc > 2:175262/1‑248 (MQ=255) gATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGTCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCaaa < 1:323895/251‑1 (MQ=255) tAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGc < 1:110636/250‑1 (MQ=255) aaaaaTACCGGAAATCCCCAAGC‑TCCATGTTCGCGCATTGGTGCCAGCCGGGATGAAGACGAAGTACCGGGCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCGGATTACGCAATCATCTTCCGAATACAGTAGCAGTTTCTGCGTTCCGCAAAGCg < 2:381568/250‑1 (MQ=255) aCCGGAAATTCTCAAGC‑TCCAGGTACGCGCATTGGTGCCAGCCGTGAAGAAGACGCATTGCCGGTCAAGGGCATTTCCAATCTGAACACTAGGGCAATGTTCAGCGTTTCCGGCCCGGGGAAGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTTCG‑TGGTGCTGATTACGCAATCATCTTCCGAATCCAGTATCAGTTTCTGCGTCCCGCAAAGCGACtgtgt < 2:533448/251‑1 (MQ=255) cgtaaaaccTCAAGC‑TCCAGGTACGCGCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAAGACAGTATCATTTTCTGCGTTCAGCAAAGCGACTGTGTGc < 2:87690/244‑1 (MQ=255) ggAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCg > 1:434441/1‑251 (MQ=255) gAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCACTCTGAATAATATGGCACTGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGAGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGCATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCgg > 1:377384/1‑250 (MQ=255) aaaTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAg < 1:414465/251‑1 (MQ=255) aaTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGTTGAAAGGGATGGTTGGCATGGCGCCGCGCGTGTTTGCAGAGATGTCACGCGCCCGTATTTCAG‑TGGTGCTGATTACGCAATCATCTTCCGAATCCAGTATCAGTTTCTGCGTTCCGCAAAGCGTCTGTGTGCGAGc > 1:382787/1‑251 (MQ=255) ccTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTTATGAAGACGAAGTACCGGTCAAGGTCATTGCCAATCTGAATAAGAGGGCAATGTTCAGCTTTTCCGGCCCGGGGATGAAAGGGGTGGTTGGCATGGCGGCTCGCGTGTTTGCAGCGATGTCACGCGCCTGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACATTATCAGTTGCTGCGTTCCGCAAAGCGACTGTGTTCGAGCTGa < 2:296005/251‑1 (MQ=255) ccAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc < 2:129594/250‑1 (MQ=255) cAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACg > 2:132323/1‑251 (MQ=255) cAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACg > 1:557567/1‑251 (MQ=255) | ACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑ACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACG > NC_000913/962‑1451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |