Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,939	G→A	T528T (ACC→ACT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,939	0	G	A	100.0%	235.6 / NA	72	T528T (ACC→ACT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/35);  total (37/35)

GTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACC  >  NC_000913/4628695‑4629181                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   gtAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTaa                                                                                                                                                                                                                                                >  2:210169/1‑249 (MQ=255)            aaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCgaa                                                                                                                                                                                                                                   <  2:163842/246‑1 (MQ=255)            aaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTAATTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCgaa                                                                                                                                                                                                                                   <  1:48748/246‑1 (MQ=255)                 gtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACt                                                                                                                                                                                                                              <  2:8248/251‑1 (MQ=255)                    cACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGCCATTTC‑TTGGTTTGTAGGCCGGATAAGACGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCa                                                                                                                                                                                                                            >  1:54991/1‑250 (MQ=255)                     ccgccgtATCCGGCATGCGTTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATGGAAATTTC‑TTGGTTTGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACGTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAAc                                                                                                                                                                                                                          <  1:555744/250‑1 (MQ=255)                     aCGCCGTATCCGGCATTCGCTGCTCAATGCATGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAAc                                                                                                                                                                                                                          >  1:488235/1‑251 (MQ=255)                      cgccgtATCCGGCATTCGGAGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCTCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACt                                                                                                                                                                                                                         >  2:371586/1‑251 (MQ=255)                       gccgtATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACtt                                                                                                                                                                                                                        >  1:105234/1‑251 (MQ=255)                            atccggctgctggTGCTCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCGTGGTTT‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATTCGCGTGTACTTGATACGCTTCGGCTCCAGTGCGGCTGCTCCCAGCGTGCGTTGCTTTTACTCTTCGTACTCAGTAAATTTACCGTCGAATAACGCATCTTTAcc                                                                                                                                                                                                                    <  2:72499/239‑1 (MQ=255)                                          gCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTagtc                                                                                                                                                                                                     <  1:163207/251‑4 (MQ=255)                                               aTGCCTGATGCGACGCTTACAGCGTCTTCTCAGGCCTGGAGATTCACACCGTATTGAAATTTC‑TTGGCTTCTAGGCCGGATAAGGCGTTCACGCCGCATACGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTACAGTTACCTTCGAAGCACTCAACTTTACCTTCCTCCTGGTAGTCCAgg                                                                                                                                                                                                 >  2:248854/1‑249 (MQ=255)                                               aTGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTGGCGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGCAGAACTCAACTTTACCTTCATCCTGGTAGTCCAgg                                                                                                                                                                                                 >  2:68144/1‑249 (MQ=255)                                                   cTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGAtgtg                                                                                                                                                                                            >  2:395388/1‑251 (MQ=255)                                                     gATGCGACGCTTACCGCGTCTGAGCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGc                                                                                                                                                                                           <  1:59369/250‑1 (MQ=255)                                                        gCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCGTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTgg                                                                                                                                                                                       <  1:264838/251‑1 (MQ=255)                                                          gACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTagt                                                                                                                                                                                                      >  1:401956/1‑232 (MQ=255)                                                          gACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCCGGTagt                                                                                                                                                                                                      <  2:401956/234‑3 (MQ=255)                                                            cGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTGCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGAt                                                                                                                                                                                   <  1:357324/251‑1 (MQ=255)                                                            cGCTTACCGCGTCTTATCAGGCCGGGAGATTCCCAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCGGCGCCCAGCGTGCGGTTCTTGTACTCTTCGTACTCAGTAAAGTGACCGTCGAAGAACTCAACTTTACCTTCATCCGGGTAGTCCAGGATGTGCGTGGCGAt                                                                                                                                                                                   <  2:580402/251‑1 (MQ=255)                                                                     cgTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGCACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCg                                                                                                                                                                            >  1:57901/1‑249 (MQ=255)                                                                      gTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAgg                                                                                                                                                                         >  2:17750/1‑251 (MQ=255)                                                                       tCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAgg                                                                                                                                                                         >  2:513705/1‑250 (MQ=255)                                                                       tCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAgg                                                                                                                                                                         >  1:484227/1‑250 (MQ=255)                                                                              aggcctggagattcaCAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACg                                                                                                                                                                 >  2:298647/15‑251 (MQ=255)                                                                              aggcctggagattaaCAACGTATTGAAATTTC‑TTGATTTGAAAGCCGGCTAAAGAGTTCACGACGCATACGGAATTTTACGCATTACTTCGCAATACGATTGTACTTGATACGCTTCGACTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGACCTCAGTAAAGATACCTTCGAAGAACTCCACTTTACCTTAATCCTGGGCGTCCAGGATTGGCGTGGCGATACGATCGAgcgaccaccg                                                                                                                                                                 >  2:350501/15‑242 (MQ=255)                                                                                        attcaCAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCgag                                                                                                                                                       <  1:442066/247‑1 (MQ=255)                                                                                                       gaaatttcttggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGcaca                                                                                                                                         <  1:169732/239‑1 (MQ=255)                                                                                                         aatttcttggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGc                                                                                                                                       >  1:38127/11‑251 (MQ=255)                                                                                                          atttcttggtttGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGcc                                                                                                                                      <  2:105234/242‑1 (MQ=255)                                                                                                             tcttggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTAGGCTCCAGTGCGTATGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCgg                                                                                                                                   >  1:438546/7‑251 (MQ=255)                                                                                                                tggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGATCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGa                                                                                                                                 >  1:559128/4‑250 (MQ=255)                                                                                                                    ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTc                                                                                                                             <  2:219658/250‑1 (MQ=255)                                                                                                                            cGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAggcc                                                                                                                    <  2:477268/251‑3 (MQ=255)                                                                                                                             ggATAAGGCGTTCCCGCCGCCTCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGACCCGCGTGCGTTTCTTGTAATCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAAATTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGCACCAACGGTCGTGCGAGATAACCATAGCACAGCCCGGGAACTCCAGCAGGGCg                                                                                                                   >  2:371932/1‑251 (MQ=255)                                                                                                                               aTAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGtt                                                                                                                 >  2:501408/1‑251 (MQ=255)                                                                                                                                   ggCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTcc                                                                                                             >  2:393528/1‑251 (MQ=255)                                                                                                                                   ggCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTcc                                                                                                             <  1:210169/251‑1 (MQ=255)                                                                                                                                         cacgccgcATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAgcgcg                                                                                                       <  2:238178/249‑1 (MQ=255)                                                                                                                                           cgccgcATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAgcgcgcg                                                                                                     >  2:62907/1‑251 (MQ=255)                                                                                                                                            gccgcATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCTCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAgcgcgcgc                                                                                                    <  1:371586/251‑1 (MQ=255)                                                                                                                                                  tCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGCTACGCTTCGGCTCCCGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGtt                                                                                              >  2:520315/1‑251 (MQ=255)                                                                                                                                                          tttACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGCTTCGATATc                                                                                      >  2:353722/1‑251 (MQ=255)                                                                                                                                                             aCGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGATTCGATATcc                                                                                     >  2:336607/1‑249 (MQ=255)                                                                                                                                                               gCATTGCTTCGCTATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTGCTTGGTCTCTTCGTACTCAGGAAAGGTACCTTCGAATAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGGGGCGATACGGTCGAGGATCCAACGGTCGTGCGAGATAACCATCGCATAGCTCGGAAACTGCAGCAGGGCGTTTTCCAGCGCGCGCATGGTGTCGATAGCCAGGt                                                                                 <  2:581536/251‑1 (MQ=255)                                                                                                                                                               gCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGa                                                                                                                                                                           <  1:263181/161‑1 (MQ=255)                                                                                                                                                               gCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGa                                                                                                                                                                           >  2:263181/1‑161 (MQ=255)                                                                                                                                                                    aCTTCTCAATACGCTTGTACTTGATACGCTGCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCgttg                                                                            <  2:12297/251‑1 (MQ=255)                                                                                                                                                                    aCTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCgttg                                                                            <  2:12283/251‑1 (MQ=255)                                                                                                                                                                          cAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTCCCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTtggttggt                                                                      <  1:185724/251‑1 (MQ=255)                                                                                                                                                                              tcgcgtGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcg                                                                   <  2:359738/245‑1 (MQ=255)                                                                                                                                                                              aCGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGAGCAGGATTTCGATATCCAGGGCGTTGGTTGGTtcgt                                                                  >  2:401007/1‑251 (MQ=255)                                                                                                                                                                               cGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCGTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcgtc                                                                 <  1:371932/251‑1 (MQ=255)                                                                                                                                                                                  ttGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTtcgtcg                                                                >  2:31945/1‑249 (MQ=255)                                                                                                                                                                                          gATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGagcagcagc                                                       <  2:228279/250‑1 (MQ=255)                                                                                                                                                                                           aTACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGGGCAGCAGCAt                                                     >  1:230386/1‑251 (MQ=255)                                                                                                                                                                                              cGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGATTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGtt                                                  <  1:401007/251‑1 (MQ=255)                                                                                                                                                                                              cGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTGTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGGTTTCCAGCGCGCGCAGGGTTTCGATATCCAGTTCGTTTGTTTGTTCGTCGAGCAGCAACATGtt                                                  <  2:392602/251‑1 (MQ=255)                                                                                                                                                                                                 ttCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTgcc                                               >  2:557084/1‑251 (MQ=255)                                                                                                                                                                                                     gCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCa                                           <  1:31945/251‑1 (MQ=255)                                                                                                                                                                                                             gcgTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTgc                                     >  1:414029/1‑249 (MQ=255)                                                                                                                                                                                                               gTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTgcagc                                  >  1:25965/1‑250 (MQ=255)                                                                                                                                                                                                                tCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTgcagc                                  >  2:215973/1‑249 (MQ=255)                                                                                                                                                                                                                   gcgcCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGcagcag                                <  1:113352/248‑1 (MQ=255)                                                                                                                                                                                                                     gcCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCg                           <  2:156055/251‑1 (MQ=255)                                                                                                                                                                                                                        cAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGcc                         >  1:102436/1‑250 (MQ=255)                                                                                                                                                                                                                              gcgtTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCa                  <  1:444608/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCCTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAgacg              >  1:444829/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGAcc           <  1:600514/250‑1 (MQ=255)                                                                                                                                                                                                                                      tGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACcg          >  1:504097/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTACGCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACcg          <  2:611553/250‑1 (MQ=255)                                                                                                                                                                                                                                              tCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTcacc  >  2:345073/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   GTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACC  >  NC_000913/4628695‑4629181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑