Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,259	T→C	T315T (ACT→ACC)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,259	0	T	C	100.0%	210.1 / NA	69	T315T (ACT→ACC)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/32);  total (37/32)

CCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCG  >  NC_000913/4628022‑4628495                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             ccTTGGTGGCGAGTCGAGCGGTAGATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTGCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGACTACGATAAAATCTCGCTGGGCCCCGCCCAATTCATTTATTTTGCAGTGAAATATGCCATTAAAGTGGCTTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCa                                                                                                                                                                                                                                 <  2:381853/251‑1 (MQ=255)     ggtggcgAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCaaaa                                                                                                                                                                                                                              <  1:134908/247‑1 (MQ=255)      gtggcgAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGGGGCGTTTATTTACACTGATTTTGTCACTACCCAGGCGTTCTGCaaaaa                                                                                                                                                                                                                             >  2:189226/3‑250 (MQ=255)             gTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAg                                                                                                                                                                                                                     <  2:161208/251‑1 (MQ=255)               cGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAggg                                                                                                                                                                                                                   <  2:429332/251‑1 (MQ=255)                gAGCGGTACATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGGGATGAGATCGCATTGCAGTATTTCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGAAAATGATTTTTTCACTACCCAGGCGCTCTGCAAAAAGTACGAAgggg                                                                                                                                                                                                                  >  2:157322/1‑250 (MQ=255)                gAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCCTTGCAGTATTTCGTTTTCGATAAAATCGCTCTGGGCCACGCCCAATACATTTATTTTGCAGTGAAATATGCCAATTAAGTGGCGTTTATTTACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAATTACGAAGGGc                                                                                                                                                                                                                  >  2:416604/1‑251 (MQ=255)                 aGCGGTAAAGCCGCCCTGGTAAGCAAACTTGCCAATATCTTCAATCCCACCAGTGCGTTGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTTCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGAGTTTGTCACTACCCAGGCGTTCTGCAAAAAGGACTAAgg                                                                                                                                                                                                                    <  2:251242/248‑1 (MQ=255)                    ggTAAATCCACCCTGGTAAACAACCTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTAAGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGGGAAATATGCCAATAAAGTGGCGTTTATTGACACCGATTTTTTCACTAACCAGGCGTTCTGCAAAAAGAACGAAGGGCgtg                                                                                                                                                                                                               >  1:171481/1‑248 (MQ=255)                    ggTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgtg                                                                                                                                                                                                               >  1:266671/1‑248 (MQ=255)                     gTAAATCCTCCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTTCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACTATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATGGACACTGATTTTGGCACTACCCAGGCGTTCTGCAAGAAGTACGAAGGTCGGGag                                                                                                                                                                                                             <  2:603462/251‑2 (MQ=255)                     gTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGAAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGAAAATGATTTTGTCACTACCCAGGGGTTCTGCAAAAAGTACGAagggaggg                                                                                                                                                                                                               >  1:72499/1‑249 (MQ=255)                         aTCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATc                                                                                                                                                                                                         >  1:244427/1‑251 (MQ=255)                                  ggTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGt                                                                                                                                                                                                  <  2:69856/249‑1 (MQ=255)                                           acTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGTATTCCGGTTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGCTTTTGTCACTACCCAGGCGTTCTGCCAAAAGTACGAAGGGCGTGAGCATCCGTTCGtcaaggcgttg                                                                                                                                                                                        >  2:195227/1‑240 (MQ=255)                                                          caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGGTGAGATCGCATTGCAGTATTCCGAATACGATAAAATCACGCTTGGCCACGCACCATACCTTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGGTTTTTGACACTAATTTTTTAACAACCCAGGCGTTCTGCAAAAAGTACAAAGGGAGCGAGCATCCGTTCGTACAGGCGTTGATTGGTGAATACCGtt                                                                                                                                                                        >  1:112635/5‑251 (MQ=255)                                                           aataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGttt                                                                                                                                                                       <  1:379554/248‑1 (MQ=255)                                                                 accaGTGCGTGGGAATCTGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGCTTATTGACACTGATTTTGTCACTAACCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCt                                                                                                                                                                 >  1:531537/1‑251 (MQ=255)                                                                      tGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGGGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATCAAGTGGCGTTTATTGACACTGATTTTGTCACAAACCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAAAAACGTTTCGATCTGGTg                                                                                                                                                             >  1:424677/1‑250 (MQ=255)                                                                         gTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATcc                                                                                                                                                         >  2:133185/1‑251 (MQ=255)                                                                            ggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAATATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGTTTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGTTTTTGGCACTACCCAGGCGTTCTGCAAAAAGTACCAAGGGCGGGAGCATCCGTTCGGACAGGCGTTGATTGATGAATACCGTTTCGGTCTGGGGATCctcg                                                                                                                                                      >  2:186740/1‑249 (MQ=255)                                                                            ggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGACTTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGAGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACCGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCctgc                                                                                                                                                      >  1:394516/1‑251 (MQ=255)                                                                               atatGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGATCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                                                                                                                                   >  1:46073/1‑251 (MQ=255)                                                                                         gATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCAGGGCCACGCACAATACATTGATTTTGCAGGGAAAAATGCCAATAACGGGGCGTTTAATGACACTGATTTTGTCACTACCCAGGCGTTCTGAAAAAAGTACGGAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGCGATCCTGCTGGAGAACAacac                                                                                                                                         >  1:54388/1‑251 (MQ=255)                                                                                           ttATGTCTTTTCACACCTCGGCGGTGATGCGATCGCATTTCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGAAGTGAAATATGCCAATAAAGTGGGGTTTATTGAAAATGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGAACGAAGGGCGGGAGCATCCGTACGGAAAGGCGGTGTTTGGTGAATACAGTTTCGATCTGGTGATCCTGCTGGAGAACCACACGc                                                                                                                                       >  2:225840/1‑251 (MQ=255)                                                                                                   tttCACACCTCGGCGGTTATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCGGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgg                                                                                                                                  <  1:514479/248‑1 (MQ=255)                                                                                                            tCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCCCAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGGGGGGGGCGGATggg                                                                                                                      >  1:373615/1‑250 (MQ=255)                                                                                                             cggcggTGAGGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACTCCGTGGGTGGCGGATGGtt                                                                                                                     <  2:500478/251‑1 (MQ=255)                                                                                                              ggcggTGATGAGATCGCATTGCATTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                                                                                                                    <  2:266671/251‑1 (MQ=255)                                                                                                              ggcggTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACCTTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                                                                                                                    >  1:589436/1‑251 (MQ=255)                                                                                                                cggTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATTCATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTGATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGGATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTTGGTGGCGTATGGTTTAc                                                                                                                  <  2:105205/251‑1 (MQ=255)                                                                                                                  gTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGc                                                                                                                >  1:474966/1‑251 (MQ=255)                                                                                                                   tgatgaGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTtgatt                                                                                                                                                                                     >  1:6614/1‑181 (MQ=255)                                                                                                                   tgatgaGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTtgatt                                                                                                                                                                                     <  2:6614/181‑1 (MQ=255)                                                                                                                       gagaTCGCATTGCAGTATGCCGATTACGATAAAATCGCGCTGGGCCACGCACAATTCATTTATTTTGCAGTGAAATATGCCAATAAAGTTGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCTTGAGCATCCGTTCGTACAGGCGTTGATTGAGGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGTCGGATGGTTTACTCAGCCt                                                                                                           <  2:388023/251‑1 (MQ=255)                                                                                                                            cGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCa                                                                                                      <  2:184955/251‑1 (MQ=255)                                                                                                                             gCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGc                                                                                                       >  1:164844/1‑249 (MQ=255)                                                                                                                              cATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTAGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGCGGGTGGCGGCTGGTTTACGCCGCCTCGGCAGt                                                                                                    >  2:464843/1‑251 (MQ=255)                                                                                                                               aTTGCAGTATTCCGATGACGATAAAATCGCGCTGGGCCACGCACAAGACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGtt                                                                                                   <  2:404129/251‑1 (MQ=255)                                                                                                                               aTTGCAGTAATCCGATTACGATAAACTCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGtt                                                                                                   >  2:328956/1‑251 (MQ=255)                                                                                                                                ttGCAGTATTCCGATTACGATACAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTc                                                                                                  >  1:160325/1‑251 (MQ=255)                                                                                                                                 tGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCAAAATACATTGATTTTGCAGTGAAATATGCCAATAAAGAGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTATGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGAGGGAGGCGGATGGTTTACGCAGCCTCGGCAGTTCg                                                                                                 >  2:163207/1‑251 (MQ=255)                                                                                                                                     gTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTgg                                                                                             <  1:133185/251‑1 (MQ=255)                                                                                                                                      gattccgattACGATAAAATCGCGCTGGGCCACGCACAATACATGGATTTTGCAGTGAAATAGGCCAATAAAGTGGCGGTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGa                                                                                            <  2:26752/242‑1 (MQ=255)                                                                                                                                       attccgattACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGa                                                                                            <  2:244427/242‑1 (MQ=255)                                                                                                                                            gattACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATTCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTTATTGATGAATACCGTTTCGATCTGGTGATCCTTCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCTTCGGCAGTTCGGTGGATCGCaa                                                                                      <  1:423587/248‑1 (MQ=255)                                                                                                                                             attACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCaa                                                                                      >  1:103129/3‑250 (MQ=255)                                                                                                                                                   aTAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTc                                                                               >  1:341776/1‑251 (MQ=255)                                                                                                                                                             gcTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGc                                                                     <  1:218600/251‑1 (MQ=255)                                                                                                                                                                gggCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCtg                                                                   <  1:416604/250‑1 (MQ=255)                                                                                                                                                                   ccACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCtggtgg                                                               <  1:611019/251‑1 (MQ=255)                                                                                                                                                                        cacAATACGTGGATTGTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGGTTCGATCTGGTGAGCCTGCTGGAGAACAACACGCCGTGGGTGGTGGAGGGTTTACGCAGCCGCGGCAGTTCGTGGGAGCGAAAAGAGTTCCAGAACTTGCGGGGGGAGATg                                                          <  2:102153/249‑1 (MQ=255)                                                                                                                                                                              ccATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTTCCCAGTCTTTCTGCAAAAAGTACGAAGGGCGTGATCATCCGTTCGTACAGGCGTTGATTGAGGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGGTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtgga                                                     <  1:219161/249‑4 (MQ=255)                                                                                                                                                                               cATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAag                                                   <  2:136359/251‑1 (MQ=255)                                                                                                                                                                                     ttttGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAAc                                              >  2:44631/1‑250 (MQ=255)                                                                                                                                                                                               aaaTATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCg                                       >  1:475409/1‑247 (MQ=255)                                                                                                                                                                                               aaaTATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCg                                       <  2:475409/247‑1 (MQ=255)                                                                                                                                                                                                aaTATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAttc                                  <  1:520622/251‑2 (MQ=255)                                                                                                                                                                                                 atatGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCTGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAttcg                                 <  1:603970/251‑3 (MQ=255)                                                                                                                                                                                                  tatGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAAttcgt                                >  1:188362/1‑248 (MQ=255)                                                                                                                                                                                                   atGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGGACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTTGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATAGCGAATTcgtg                               <  2:589436/251‑1 (MQ=255)                                                                                                                                                                                                    tGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGc                              >  1:442930/1‑251 (MQ=255)                                                                                                                                                                                                     gCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCCCTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGc                              <  2:375723/250‑1 (MQ=255)                                                                                                                                                                                                       cAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCggg                           <  2:239909/251‑1 (MQ=255)                                                                                                                                                                                                               tGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAag                     >  1:163842/1‑249 (MQ=255)                                                                                                                                                                                                               tGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTAGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAag                     >  2:264838/1‑249 (MQ=255)                                                                                                                                                                                                                ggcgattatttacactgcttttGTCACTACCCAGGCGTTCTGCAGAAATTACGAAGGGCGTGAGCATCCCTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCAGCTGGAGAACAACCCGCCGTGGGTGTCGGATGGTTTACGCAGCCTCGACATCTCGGTGGATCGAAAAGAGTTCCTGAACTTGCTTGTGGAGGTTCTGGAAGAGCACAATCTCGAATTCGTGCGGGTTAAAGAgg                  >  2:59369/19‑251 (MQ=255)                                                                                                                                                                                                                               tgATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAgcc   >  2:238162/2‑249 (MQ=255)                                                                                                                                                                                                                                gATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGCATTCGTGCGGGTTGAAGAGGCAGATTATGACAgccg  >  2:80080/1‑248 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             CCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCG  >  NC_000913/4628022‑4628495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑