Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,208	G→A	A298A (GCG→GCA)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,208	0	G	A	100.0%	204.6 / NA	70	A298A (GCG→GCA)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/34);  total (36/34)

GAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAAC  >  NC_000913/4627972‑4628451                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    gAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCa                                                                                                                                                                                                                                        >  1:184955/1‑250 (MQ=255)     atatTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAAGACATTGATTTTGCAGTGAAATATGCCAATaaa                                                                                                                                                                                                                                   <  2:601712/251‑1 (MQ=255)         tCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTgg                                                                                                                                                                                                                               >  1:569248/1‑251 (MQ=255)          ccTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATTCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGAGTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGc                                                                                                                                                                                                                              <  1:53132/251‑1 (MQ=255)             acggAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACTCACAATACATTTATTTTGCAGTGAAATATGCCAATAAAGTGGcgtt                                                                                                                                                                                                                           <  1:230320/248‑4 (MQ=255)             acggAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAAACGCGCTGGGCCACGCACAATACATTTGTTTTGCAGGGAAATATGCCACTAAAggtgcggt                                                                                                                                                                                                                           >  2:14936/4‑244 (MQ=255)                  aGTGACGCCGTTCTTTGTACGAACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCACTATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACAACTCGGCGGGGATGGAATCGCAATGCAGTATTCCGATTCCGATAAAATCGCGACGGGCCAAGACCAATACCTTGATTTTGCAATGAAATAAGCCacaacaggggcgtttattt                                                                                                                                                                                                                      >  2:603970/1‑233 (MQ=255)                  aGTGAAGCCGTTCTTTGTACGTACCGTGGCGAGCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGGAAACAAACGTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTAttg                                                                                                                                                                                                                      <  1:22767/251‑3 (MQ=255)                         ccGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGCTTACGGTAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTAttgaaactt                                                                                                                                                                                                                >  1:504760/1‑242 (MQ=255)                           gttctttGTCCGTACCGAGGCGATACTTGGTGGCGAGTCGCGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATCCCACCAGTGCGTGGGAATATGGTCGCGCTTATGTCTTTTCACACCTCGGCGGTGATGAACTCGCCATGCAGTATTTCGATTACGATCAAAACGCGCTGGGCCACGAAACATACATTGATTTTGAAGTGAAAAATTCCAATAAAGTGGCGTTTAttgtcaatgatt                                                                                                                                                                                                             >  2:308118/5‑240 (MQ=255)                             tctttGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGAttt                                                                                                                                                                                                            <  2:85747/248‑1 (MQ=255)                                  gtacgttccgaggCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACCATACATTGAGTTTGCAGTGAAATATTCCAATAAAGTGCCGTTTATTGACCCTGATTTTGTct                                                                                                                                                                                                       <  2:368552/239‑2 (MQ=255)                                     cGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTcactac                                                                                                                                                                                                   >  1:198565/1‑248 (MQ=255)                                        aCCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTcactaccc                                                                                                                                                                                                 <  1:475905/250‑6 (MQ=255)                                          cgtcgCGAACCTTGGTGGCGAGTCGAACGGTCAATCCACCCTGGTAAACAAACTTTTCAATATCTTCAATACCAACATTTCGTGGAAATATGGTCGCTATTATGTCTTTTCACACCTCGGCGGTGATGATATCGCATTGAAGTATTCCGACTACGATAAAATCGCGCTGGGCCACGCACAATACACTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTctctaccctgg                                                                                                                                                                                              <  2:485653/247‑11 (MQ=255)                                                   ccTTGGTGGCGAGTCGAGCGGTAGATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTGCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGACTACGATAAAATCTCGCTGGGCCCCGCCCAATTCATTTATTTTGCAGTGAAATATGCCATTAAAGTGGCTTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCa                                                                                                                                                                                     <  2:381853/251‑1 (MQ=255)                                                       ggtggcgAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCaaaa                                                                                                                                                                                  <  1:134908/247‑1 (MQ=255)                                                        gtggcgAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGGGGCGTTTATTTACACTGATTTTGTCACTACCCAGGCGTTCTGCaaaaa                                                                                                                                                                                 >  2:189226/3‑250 (MQ=255)                                                               gTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAg                                                                                                                                                                         <  2:161208/251‑1 (MQ=255)                                                                 cGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAggg                                                                                                                                                                       <  2:429332/251‑1 (MQ=255)                                                                  gAGCGGTACATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGGGATGAGATCGCATTGCAGTATTTCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGAAAATGATTTTTTCACTACCCAGGCGCTCTGCAAAAAGTACGAAgggg                                                                                                                                                                      >  2:157322/1‑250 (MQ=255)                                                                  gAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCCTTGCAGTATTTCGTTTTCGATAAAATCGCTCTGGGCCACGCCCAATACATTTATTTTGCAGTGAAATATGCCAATTAAGTGGCGTTTATTTACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAATTACGAAGGGc                                                                                                                                                                      >  2:416604/1‑251 (MQ=255)                                                                   aGCGGTAAAGCCGCCCTGGTAAGCAAACTTGCCAATATCTTCAATCCCACCAGTGCGTTGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTTCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGAGTTTGTCACTACCCAGGCGTTCTGCAAAAAGGACTAAgg                                                                                                                                                                        <  2:251242/248‑1 (MQ=255)                                                                      ggTAAATCCACCCTGGTAAACAACCTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTAAGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGGGAAATATGCCAATAAAGTGGCGTTTATTGACACCGATTTTTTCACTAACCAGGCGTTCTGCAAAAAGAACGAAGGGCgtg                                                                                                                                                                   >  1:171481/1‑248 (MQ=255)                                                                      ggTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgtg                                                                                                                                                                   >  1:266671/1‑248 (MQ=255)                                                                       gTAAATCCTCCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTTCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACTATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATGGACACTGATTTTGGCACTACCCAGGCGTTCTGCAAGAAGTACGAAGGTCGGGag                                                                                                                                                                 <  2:603462/251‑2 (MQ=255)                                                                       gTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGAAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGAAAATGATTTTGTCACTACCCAGGGGTTCTGCAAAAAGTACGAagggaggg                                                                                                                                                                   >  1:72499/1‑249 (MQ=255)                                                                           aTCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATc                                                                                                                                                             >  1:244427/1‑251 (MQ=255)                                                                                    ggTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGt                                                                                                                                                      <  2:69856/249‑1 (MQ=255)                                                                                             acTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGTATTCCGGTTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGCTTTTGTCACTACCCAGGCGTTCTGCCAAAAGTACGAAGGGCGTGAGCATCCGTTCGtcaaggcgttg                                                                                                                                            >  2:195227/1‑240 (MQ=255)                                                                                                            caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGGTGAGATCGCATTGCAGTATTCCGAATACGATAAAATCACGCTTGGCCACGCACCATACCTTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGGTTTTTGACACTAATTTTTTAACAACCCAGGCGTTCTGCAAAAAGTACAAAGGGAGCGAGCATCCGTTCGTACAGGCGTTGATTGGTGAATACCGtt                                                                                                                            >  1:112635/5‑251 (MQ=255)                                                                                                             aataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGttt                                                                                                                           <  1:379554/248‑1 (MQ=255)                                                                                                                   accaGTGCGTGGGAATCTGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGCTTATTGACACTGATTTTGTCACTAACCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCt                                                                                                                     >  1:531537/1‑251 (MQ=255)                                                                                                                        tGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGGGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATCAAGTGGCGTTTATTGACACTGATTTTGTCACAAACCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAAAAACGTTTCGATCTGGTg                                                                                                                 >  1:424677/1‑250 (MQ=255)                                                                                                                           gTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATcc                                                                                                             >  2:133185/1‑251 (MQ=255)                                                                                                                              ggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAATATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGTTTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGTTTTTGGCACTACCCAGGCGTTCTGCAAAAAGTACCAAGGGCGGGAGCATCCGTTCGGACAGGCGTTGATTGATGAATACCGTTTCGGTCTGGGGATCctcg                                                                                                          >  2:186740/1‑249 (MQ=255)                                                                                                                              ggAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGACTTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGAGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACCGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCctgc                                                                                                          >  1:394516/1‑251 (MQ=255)                                                                                                                                 atatGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGATCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTgg                                                                                                       >  1:46073/1‑251 (MQ=255)                                                                                                                                           gATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCAGGGCCACGCACAATACATTGATTTTGCAGGGAAAAATGCCAATAACGGGGCGTTTAATGACACTGATTTTGTCACTACCCAGGCGTTCTGAAAAAAGTACGGAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGCGATCCTGCTGGAGAACAacac                                                                                             >  1:54388/1‑251 (MQ=255)                                                                                                                                             ttATGTCTTTTCACACCTCGGCGGTGATGCGATCGCATTTCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGAAGTGAAATATGCCAATAAAGTGGGGTTTATTGAAAATGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGAACGAAGGGCGGGAGCATCCGTACGGAAAGGCGGTGTTTGGTGAATACAGTTTCGATCTGGTGATCCTGCTGGAGAACCACACGc                                                                                           >  2:225840/1‑251 (MQ=255)                                                                                                                                                     tttCACACCTCGGCGGTTATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCGGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCgtgg                                                                                      <  1:514479/248‑1 (MQ=255)                                                                                                                                                              tCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCCCAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGGGGGGGGCGGATggg                                                                          >  1:373615/1‑250 (MQ=255)                                                                                                                                                               cggcggTGAGGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACTCCGTGGGTGGCGGATGGtt                                                                         <  2:500478/251‑1 (MQ=255)                                                                                                                                                                ggcggTGATGAGATCGCATTGCATTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                                                                        <  2:266671/251‑1 (MQ=255)                                                                                                                                                                ggcggTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACCTTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGttt                                                                        >  1:589436/1‑251 (MQ=255)                                                                                                                                                                  cggTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATTCATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTGATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGGATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTTGGTGGCGTATGGTTTAc                                                                      <  2:105205/251‑1 (MQ=255)                                                                                                                                                                    gTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGc                                                                    >  1:474966/1‑251 (MQ=255)                                                                                                                                                                     tgatgaGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTtgatt                                                                                                                                         >  1:6614/1‑181 (MQ=255)                                                                                                                                                                     tgatgaGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTtgatt                                                                                                                                         <  2:6614/181‑1 (MQ=255)                                                                                                                                                                         gagaTCGCATTGCAGTATGCCGATTACGATAAAATCGCGCTGGGCCACGCACAATTCATTTATTTTGCAGTGAAATATGCCAATAAAGTTGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCTTGAGCATCCGTTCGTACAGGCGTTGATTGAGGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGTCGGATGGTTTACTCAGCCt                                                               <  2:388023/251‑1 (MQ=255)                                                                                                                                                                              cGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCa                                                          <  2:184955/251‑1 (MQ=255)                                                                                                                                                                               gCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGc                                                           >  1:164844/1‑249 (MQ=255)                                                                                                                                                                                cATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTAGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGCGGGTGGCGGCTGGTTTACGCCGCCTCGGCAGt                                                        >  2:464843/1‑251 (MQ=255)                                                                                                                                                                                 aTTGCAGTATTCCGATGACGATAAAATCGCGCTGGGCCACGCACAAGACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGtt                                                       <  2:404129/251‑1 (MQ=255)                                                                                                                                                                                 aTTGCAGTAATCCGATTACGATAAACTCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGtt                                                       >  2:328956/1‑251 (MQ=255)                                                                                                                                                                                  ttGCAGTATTCCGATTACGATACAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTc                                                      >  1:160325/1‑251 (MQ=255)                                                                                                                                                                                   tGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCAAAATACATTGATTTTGCAGTGAAATATGCCAATAAAGAGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTATGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGAGGGAGGCGGATGGTTTACGCAGCCTCGGCAGTTCg                                                     >  2:163207/1‑251 (MQ=255)                                                                                                                                                                                       gTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTgg                                                 <  1:133185/251‑1 (MQ=255)                                                                                                                                                                                        gattccgattACGATAAAATCGCGCTGGGCCACGCACAATACATGGATTTTGCAGTGAAATAGGCCAATAAAGTGGCGGTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGa                                                <  2:26752/242‑1 (MQ=255)                                                                                                                                                                                         attccgattACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGa                                                <  2:244427/242‑1 (MQ=255)                                                                                                                                                                                              gattACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATTCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTTATTGATGAATACCGTTTCGATCTGGTGATCCTTCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCTTCGGCAGTTCGGTGGATCGCaa                                          <  1:423587/248‑1 (MQ=255)                                                                                                                                                                                               attACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCaa                                          >  1:103129/3‑250 (MQ=255)                                                                                                                                                                                                     aTAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTc                                   >  1:341776/1‑251 (MQ=255)                                                                                                                                                                                                               gcTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGc                         <  1:218600/251‑1 (MQ=255)                                                                                                                                                                                                                  gggCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCtg                       <  1:416604/250‑1 (MQ=255)                                                                                                                                                                                                                     ccACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCtggtgg                   <  1:611019/251‑1 (MQ=255)                                                                                                                                                                                                                          cacAATACGTGGATTGTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGGTTCGATCTGGTGAGCCTGCTGGAGAACAACACGCCGTGGGTGGTGGAGGGTTTACGCAGCCGCGGCAGTTCGTGGGAGCGAAAAGAGTTCCAGAACTTGCGGGGGGAGATg              <  2:102153/249‑1 (MQ=255)                                                                                                                                                                                                                                ccATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTTCCCAGTCTTTCTGCAAAAAGTACGAAGGGCGTGATCATCCGTTCGTACAGGCGTTGATTGAGGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGGTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtgga         <  1:219161/249‑4 (MQ=255)                                                                                                                                                                                                                                 cATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAag       <  2:136359/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttttGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAAc  >  2:44631/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    GAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAAC  >  NC_000913/4627972‑4628451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑