Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,833	T→C	D173D (GAT→GAC)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,833	0	T	C	100.0%	163.8 / NA	54	D173D (GAT→GAC)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/21);  total (33/21)

GCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTT  >  NC_000913/4627593‑4628078                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      acTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACATGATCTac                                                                                                                                                                                                                                              >  1:324313/2‑250 (MQ=255)  cTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTacac                                                                                                                                                                                                                                            >  1:523224/1‑251 (MQ=255)             tATGGGTTTTGACGATACCCGCGATCGCGCGTTGTGTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGTCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGACTCAGCCGGACCTGATCTACACCTCGgaagaa                                                                                                                                                                                                                                  <  2:336201/250‑1 (MQ=255)                  gTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCCTGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGAt                                                                                                                                                                                                                            >  1:571692/1‑251 (MQ=255)                   ttttGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATg                                                                                                                                                                                                                           >  1:504165/1‑251 (MQ=255)                         cGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCcac                                                                                                                                                                                                                     <  2:523224/251‑1 (MQ=255)                                   gatcgcgcgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATgg                                                                                                                                                                                                            >  1:69856/4‑250 (MQ=255)                                    atcgcgcgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAACAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCAAAGTATATGGa                                                                                                                                                                                                           >  2:129937/3‑250 (MQ=255)                                          cgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTATCCGCACGGCTGGGATGTGGGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTg                                                                                                                                                                                                    >  1:225938/1‑251 (MQ=255)                                              gtttgAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTAATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGAAACGGAATCAAAAAGTTTATGGCTGATAAGGGGATTCAGCCGGACCTGATCTAAACCTCGGAAGCAGCCGATGAGCCCCAGTATATGGAACCTCTgggg                                                                                                                                                                                                 >  2:520622/5‑250 (MQ=255)                                                 tgaagacatacctaTGTCGCAGCAGCCCACCGTGCCGGATCGGCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCCTTCATGCTTTCATCGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATAAAAACCATTATCGCTGAAAAGGGGATTCAGCCGGACCTGATATACACCTCGGAAAAAGCCGTTGCGCCACAGTATATGGGACATCTgtgttccg                                                                                                                                                                                             >  2:22767/14‑244 (MQ=255)                                                     gACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCgatac                                                                                                                                                                                         >  1:415687/1‑248 (MQ=255)                                                             taTGTCGCAGCAGCCCAACGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAAAGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTgg                                                                                                                                                                                 >  2:328719/2‑251 (MQ=255)                                                              atatCGCAGCAGCCCTCCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAACGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGt                                                                                                                                                                                >  2:514479/4‑251 (MQ=255)                                                                    cagcagCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATcc                                                                                                                                                                          <  1:387009/251‑1 (MQ=255)                                                                            caCCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATGCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGTATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTa                                                                                                                                                                  <  1:369509/250‑1 (MQ=255)                                                                                gTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCtt                                                                                                                                                              >  2:134908/1‑251 (MQ=255)                                                                                gTGCCGGAGCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCtt                                                                                                                                                              >  2:230320/1‑251 (MQ=255)                                                                                           tCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGAACCTTTATGAGTATc                                                                                                                                                    >  1:105205/1‑250 (MQ=255)                                                                                            cTGCGCTGGTTATTACCAACTTTTAAATATCAGAAAAATCTTCGCCTTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCCACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATCTGGAACATCTGGGGATCGATACGGCGCTGGTCGATCCGAAACGTACCTTTATGAGTATc                                                                                                                                                    >  2:520383/1‑249 (MQ=255)                                                                                                       attacAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAg                                                                                                                                        <  1:173603/246‑1 (MQ=255)                                                                                                          acAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATcc                                                                                                                                    <  1:120830/250‑1 (MQ=255)                                                                                                            aaaCTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCcgc                                                                                                                                  >  1:102153/1‑251 (MQ=255)                                                                                                                    aaaTATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATgcg                                                                                                                                                                                                                         <  1:462003/156‑1 (MQ=255)                                                                                                                    aaaTATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATgcg                                                                                                                                                                                                                         >  2:462003/1‑156 (MQ=255)                                                                                                                       tatCAGATAAATTTTCGAATTCATGCTTTCAACGAAGAGGGCATGGAGCAGTATCCGCACGGCTGGGTTGAGTGGAGCAACGGCATAAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACATGATCTACACATCGGAAGGAGCCGATGCGCCACAGTATATGGACCATCTGGGGATCGATACGGTGCTGTTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGtt                                                                                                                       >  2:273899/1‑251 (MQ=255)                                                                                                                            gAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCt                                                                                                                  <  1:148541/251‑1 (MQ=255)                                                                                                                                aaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTg                                                                                                              <  2:324313/251‑1 (MQ=255)                                                                                                                                  tatTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCAGCGAAAACCCGTTCCGCTACTggg                                                                                                            >  1:251242/1‑251 (MQ=255)                                                                                                                                         attcattcTTTCAACGAGGAGGGCTGGGAGCCTGATCCGCACGTGTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCCGACTTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTAGATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGGACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGGTTCGCTACTTGGAatatat                                                                                                     <  2:416836/251‑1 (MQ=255)                                                                                                                                                cTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTAcg                                                                                              >  2:218600/1‑250 (MQ=255)                                                                                                                                                  ttCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTAcgg                                                                                             <  2:504165/250‑3 (MQ=255)                                                                                                                                                         aaGAGGGCATGGAGCCGTATCCGCACGGCTGTGATGTGGGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAg                                                                                     <  2:563007/251‑1 (MQ=255)                                                                                                                                                         aaGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAAcc                                                                                                                           <  2:397143/213‑1 (MQ=255)                                                                                                                                                         aaGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAAcc                                                                                                                           >  1:397143/1‑213 (MQ=255)                                                                                                                                                                cATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGACGTGAAGCCGttct                                                                              >  2:520433/1‑249 (MQ=255)                                                                                                                                                                       ccGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTtgtacg                                                                       <  1:23708/251‑4 (MQ=255)                                                                                                                                                                          tATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAACGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACCCCTCGGAAGGAGCCGCTGCGCCACAGAATATGGAACATCTGGGGATCGAAACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATACGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGttctttttacgtcc                                                                    >  2:611019/1‑243 (MQ=255)                                                                                                                                                                              cGCACGGCTGGGATGCGTGGAGAAACGGCATCAAAAAGTTTATGGCTGAACAGGGGATTCAGCCGGCCCTGCTCTACACCTCGGAAGAAGCCGATGCGCGACAGTATCTGGAACATCTGGGGCTCGAAAAGGTGCTGGTAGATCCGAAACGTACGTTTCTGAGTATCAGCGGTGCGCAGATCCGCGACAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGCTCTTTGTACGTACCGTg                                                                >  1:406243/1‑251 (MQ=255)                                                                                                                                                                                       gggATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAATCCGTTCTTTGTACGTACCGTGGCGATCCtt                                                       <  1:119209/251‑1 (MQ=255)                                                                                                                                                                                       gggATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCtt                                                       <  1:246801/251‑1 (MQ=255)                                                                                                                                                                                       gggATGTGTGGAGCAACGGCATAAAACAGTTTATAGCTGAAAAGGGGATTGAGCCGGACCTGATCTACACCTAAGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATAGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGGAAACCCGTTCCGCTACTGGGAATATATTCCTACGGCAGTGACGCCGTTCTTTGTACGTACCGTGACGATCCtt                                                       >  2:423587/1‑251 (MQ=255)                                                                                                                                                                                         gATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAAAGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTCCTGGGCATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTgg                                                     >  1:381853/1‑251 (MQ=255)                                                                                                                                                                                            gtggcgagtaaCGGAATCAGAAAGTTTATGGTTGATAAGGGGAATCAGCCGGACCTGATCTACACCTGGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTTAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTggtg                                                   <  2:597657/241‑3 (MQ=255)                                                                                                                                                                                                     aaCGGCATCAAAAAGTTTATGGCTGAAAAGGGGGTTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCg                                           >  2:339640/1‑249 (MQ=255)                                                                                                                                                                                                                  aGTTGATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTAGCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCTAGCGGTAAATCCAcc                            <  1:520383/251‑1 (MQ=255)                                                                                                                                                                                                                   gTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGCAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACAGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAccc                           >  1:359585/1‑251 (MQ=255)                                                                                                                                                                                                                   gTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAccc                           >  1:404129/1‑251 (MQ=255)                                                                                                                                                                                                                        tGGCTGCAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGt                       >  2:597992/1‑250 (MQ=255)                                                                                                                                                                                                                                  ggggATTCAGCCGGACCTGATCTACACCTCGGAAGCAGCCGATGCGCCACAGTATATGGCACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGAGGCGATCCTTGGTGGCGAGACGCGCGGGAAATCAACCCTGGTAAACAAACTTg            >  2:379554/2‑251 (MQ=255)                                                                                                                                                                                                                                         cAGCCGGACCTTATCTACACCGCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCTGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCGACTGGGAATATATTCCTACGGAAGTTAAGCCGTGCTTTGTACGTACCGTGGCGATCCTGGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAatat     <  1:565385/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCt   <  1:188088/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACAGCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGAAGATCCGCGAAAACCCGTGCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACTTTCCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCt   <  1:598677/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCtt  >  1:388023/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTT  >  NC_000913/4627593‑4628078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑