Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,818	A→G	K168K (AAA→AAG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,818	0	A	G	100.0%	167.0 / NA	51	K168K (AAA→AAG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/18);  total (33/18)

GTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGT  >  NC_000913/4627585‑4628057                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ggtGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGAAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGgacct                                                                                                                                                                                                                                >  1:368552/3‑248 (MQ=255) gTTGACGAACTGCATATCATTATGGGTTTTGACGATACCAGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCGTTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCACAAAGTTTATGGCTGCAAAGGGGATTCAGACGgacct                                                                                                                                                                                                                                >  1:603462/1‑248 (MQ=255)         acTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACATGATCTac                                                                                                                                                                                                                         >  1:324313/2‑250 (MQ=255)          cTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTacac                                                                                                                                                                                                                       >  1:523224/1‑251 (MQ=255)                     tATGGGTTTTGACGATACCCGCGATCGCGCGTTGTGTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGTCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGACTCAGCCGGACCTGATCTACACCTCGgaagaa                                                                                                                                                                                                             <  2:336201/250‑1 (MQ=255)                          gTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCCTGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGAt                                                                                                                                                                                                       >  1:571692/1‑251 (MQ=255)                           ttttGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATg                                                                                                                                                                                                      >  1:504165/1‑251 (MQ=255)                                 cGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCcac                                                                                                                                                                                                <  2:523224/251‑1 (MQ=255)                                           gatcgcgcgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATgg                                                                                                                                                                                       >  1:69856/4‑250 (MQ=255)                                            atcgcgcgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAACAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCAAAGTATATGGa                                                                                                                                                                                      >  2:129937/3‑250 (MQ=255)                                                  cgTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGAATGGAGCCGTATCCGCACGGCTGGGATGTGGGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTg                                                                                                                                                                               >  1:225938/1‑251 (MQ=255)                                                      gtttgAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTAATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGAAACGGAATCAAAAAGTTTATGGCTGATAAGGGGATTCAGCCGGACCTGATCTAAACCTCGGAAGCAGCCGATGAGCCCCAGTATATGGAACCTCTgggg                                                                                                                                                                            >  2:520622/5‑250 (MQ=255)                                                         tgaagacatacctaTGTCGCAGCAGCCCACCGTGCCGGATCGGCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCCTTCATGCTTTCATCGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATAAAAACCATTATCGCTGAAAAGGGGATTCAGCCGGACCTGATATACACCTCGGAAAAAGCCGTTGCGCCACAGTATATGGGACATCTgtgttccg                                                                                                                                                                        >  2:22767/14‑244 (MQ=255)                                                             gACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCgatac                                                                                                                                                                    >  1:415687/1‑248 (MQ=255)                                                                     taTGTCGCAGCAGCCCAACGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAAAGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTgg                                                                                                                                                            >  2:328719/2‑251 (MQ=255)                                                                      atatCGCAGCAGCCCTCCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAACGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGt                                                                                                                                                           >  2:514479/4‑251 (MQ=255)                                                                            cagcagCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATcc                                                                                                                                                     <  1:387009/251‑1 (MQ=255)                                                                                    caCCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATGCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGTATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTa                                                                                                                                             <  1:369509/250‑1 (MQ=255)                                                                                        gTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCtt                                                                                                                                         >  2:134908/1‑251 (MQ=255)                                                                                        gTGCCGGAGCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCtt                                                                                                                                         >  2:230320/1‑251 (MQ=255)                                                                                                   tCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGAACCTTTATGAGTATc                                                                                                                               >  1:105205/1‑250 (MQ=255)                                                                                                    cTGCGCTGGTTATTACCAACTTTTAAATATCAGAAAAATCTTCGCCTTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCCACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATCTGGAACATCTGGGGATCGATACGGCGCTGGTCGATCCGAAACGTACCTTTATGAGTATc                                                                                                                               >  2:520383/1‑249 (MQ=255)                                                                                                               attacAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAg                                                                                                                   <  1:173603/246‑1 (MQ=255)                                                                                                                  acAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATcc                                                                                                               <  1:120830/250‑1 (MQ=255)                                                                                                                    aaaCTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCcgc                                                                                                             >  1:102153/1‑251 (MQ=255)                                                                                                                            aaaTATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATgcg                                                                                                                                                                                                    >  2:462003/1‑156 (MQ=255)                                                                                                                            aaaTATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATgcg                                                                                                                                                                                                    <  1:462003/156‑1 (MQ=255)                                                                                                                               tatCAGATAAATTTTCGAATTCATGCTTTCAACGAAGAGGGCATGGAGCAGTATCCGCACGGCTGGGTTGAGTGGAGCAACGGCATAAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACATGATCTACACATCGGAAGGAGCCGATGCGCCACAGTATATGGACCATCTGGGGATCGATACGGTGCTGTTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGtt                                                                                                  >  2:273899/1‑251 (MQ=255)                                                                                                                                    gAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCt                                                                                             <  1:148541/251‑1 (MQ=255)                                                                                                                                        aaTATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTg                                                                                         <  2:324313/251‑1 (MQ=255)                                                                                                                                          tatTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCAGCGAAAACCCGTTCCGCTACTggg                                                                                       >  1:251242/1‑251 (MQ=255)                                                                                                                                                 attcattcTTTCAACGAGGAGGGCTGGGAGCCTGATCCGCACGTGTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCCGACTTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTAGATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGGACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGGTTCGCTACTTGGAatatat                                                                                <  2:416836/251‑1 (MQ=255)                                                                                                                                                        cTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTAcg                                                                         >  2:218600/1‑250 (MQ=255)                                                                                                                                                          ttCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTAcgg                                                                        <  2:504165/250‑3 (MQ=255)                                                                                                                                                                 aaGAGGGCATGGAGCCGTATCCGCACGGCTGTGATGTGGGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAg                                                                <  2:563007/251‑1 (MQ=255)                                                                                                                                                                 aaGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAAcc                                                                                                      >  1:397143/1‑213 (MQ=255)                                                                                                                                                                 aaGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAAcc                                                                                                      <  2:397143/213‑1 (MQ=255)                                                                                                                                                                        cATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGACGTGAAGCCGttct                                                         >  2:520433/1‑249 (MQ=255)                                                                                                                                                                               ccGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTtgtacg                                                  <  1:23708/251‑4 (MQ=255)                                                                                                                                                                                  tATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAACGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACCCCTCGGAAGGAGCCGCTGCGCCACAGAATATGGAACATCTGGGGATCGAAACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATACGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGttctttttacgtcc                                               >  2:611019/1‑243 (MQ=255)                                                                                                                                                                                      cGCACGGCTGGGATGCGTGGAGAAACGGCATCAAAAAGTTTATGGCTGAACAGGGGATTCAGCCGGCCCTGCTCTACACCTCGGAAGAAGCCGATGCGCGACAGTATCTGGAACATCTGGGGCTCGAAAAGGTGCTGGTAGATCCGAAACGTACGTTTCTGAGTATCAGCGGTGCGCAGATCCGCGACAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGCTCTTTGTACGTACCGTg                                           >  1:406243/1‑251 (MQ=255)                                                                                                                                                                                               gggATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAATCCGTTCTTTGTACGTACCGTGGCGATCCtt                                  <  1:119209/251‑1 (MQ=255)                                                                                                                                                                                               gggATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCtt                                  <  1:246801/251‑1 (MQ=255)                                                                                                                                                                                               gggATGTGTGGAGCAACGGCATAAAACAGTTTATAGCTGAAAAGGGGATTGAGCCGGACCTGATCTACACCTAAGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATAGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGGAAACCCGTTCCGCTACTGGGAATATATTCCTACGGCAGTGACGCCGTTCTTTGTACGTACCGTGACGATCCtt                                  >  2:423587/1‑251 (MQ=255)                                                                                                                                                                                                 gATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAAAGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTCCTGGGCATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTgg                                >  1:381853/1‑251 (MQ=255)                                                                                                                                                                                                    gtggcgagtaaCGGAATCAGAAAGTTTATGGTTGATAAGGGGAATCAGCCGGACCTGATCTACACCTGGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTTAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTggtg                              <  2:597657/241‑3 (MQ=255)                                                                                                                                                                                                             aaCGGCATCAAAAAGTTTATGGCTGAAAAGGGGGTTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCg                      >  2:339640/1‑249 (MQ=255)                                                                                                                                                                                                                          aGTTGATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTAGCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCTAGCGGTAAATCCAcc       <  1:520383/251‑1 (MQ=255)                                                                                                                                                                                                                           gTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGCAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACAGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAccc      >  1:359585/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAccc      >  1:404129/1‑251 (MQ=255)                                                                                                                                                                                                                                tGGCTGCAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGATACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACGGAAGTGAAGCCGTTCTTTGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGt  >  2:597992/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                GTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGT  >  NC_000913/4627585‑4628057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑