Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,898	A→G	L354L (CTT→CTC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,898	0	A	G	100.0%	147.1 / NA	49	L354L (CTT→CTC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/14);  total (35/14)

AGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AGCACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCA  >  NC_000913/6664‑7136                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  aGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTc                                                                                                                                                                                                                                     >  1:230600/1‑249 (MQ=255)     gagaGCAGTAAAATGGCGGTCAAATTGGTAAGAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCAcc                                                                                                                                                                                                                               <  2:75257/251‑1 (MQ=255)               aaaTGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAAccc                                                                                                                                                                                                                     >  2:542506/1‑251 (MQ=255)                aaTGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGATGGTAACAAACTCAGCACCCCAGGAACCCa                                                                                                                                                                                                                    <  2:308851/251‑1 (MQ=255)                   ggCGGTAAAATTGGTAATAGCCATGCAGGCCCTTATGATATCGGGCAGTTAACACATCAGCGGAAGTATTCGCCTGATGACGCCGATGAACGTTGCGAAGGTGCAGATCCGCAAACACAAGATAGCTTTAGGGTTGTTCTGGCGTACACAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAATAGAATAACCACGAGGCTGACAAACTCAGCACCCCAGGAACCCAtt                                                                                                                                                                                                                  >  2:59482/1‑250 (MQ=255)                    gCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGACGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑a                                                                                                                                                                                                                >  1:272819/1‑250 (MQ=255)                      ggTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCACAGGAACCCATT‑aac                                                                                                                                                                                                              >  1:51876/1‑248 (MQ=255)                      ggTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGGTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAAc                                                                                                                                                                                                                                           >  1:426211/1‑222 (MQ=255)                                     aGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGTGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTc                                                                                                                                                                                               <  1:550022/250‑1 (MQ=255)                                     aGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCAACCCAGGAACCCATT‑AACACCCGCATCGCCTTCt                                                                                                                                                                                              >  1:19329/1‑251 (MQ=255)                                     aGCCATGCAGGCCATTATGATAGCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCAACGCCTTc                                                                                                                                                                                               <  2:2197/250‑1 (MQ=255)                                            cAGGCCATTACGATCTCTGCCGGTTGCCACATCAGCGGAATACTTAGCAAGGTGACGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGCTCGCTTTAGGGATGTTCCGGCGTACAAAGCAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGAAAACAGAATAACCACGAGGGGAACAAACTCAGCACCCCAGGAACCCATT‑AACACCGGCATCGCCTTCTGGA‑Ta                                                                                                                                                                                        >  2:304487/1‑249 (MQ=255)                                                          atCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCAGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGAAAAAAACTAAGCACCCCAGGA‑CCCATTTAACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTcc                                                                                                                                                                         >  1:572259/1‑250 (MQ=255)                                                              gCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGAGCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCgg                                                                                                                                                                    <  1:399113/251‑1 (MQ=255)                                                                cAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCa                                                                                                                                                                  <  1:155584/251‑1 (MQ=255)                                                                 aGTTGCCACATCAGCGGACGCCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCAt                                                                                                                                                                 >  1:472022/1‑251 (MQ=255)                                                                 aGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCAt                                                                                                                                                                 >  2:394866/1‑251 (MQ=255)                                                                          aTCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTtgt                                                                                                                                                        >  1:136287/1‑251 (MQ=255)                                                                                     acTTAGAAAGGCGCCGCCGCTGACCGTAGCGCAGGGGCAGAACCGCAAAGACCCGCTCGCTTCAGGGTTGTTCAGGCATAAAAAGAAGAGATTGCTTTA‑GGCGTAAATGAAGTTGGCAACGATGGAGATGAAGGCAAACAGAATAACCACGAGGGAAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGATTGTGCCGTT‑AACCg                                                                                                                                            >  2:410081/2‑251 (MQ=255)                                                                                           cAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGt                                                                                                                                       >  2:219594/1‑250 (MQ=255)                                                                                                    gccgATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGc                                                                                                                              >  1:306763/1‑250 (MQ=255)                                                                                                           aCCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGAATGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCt                                                                                                                      >  1:169411/1‑251 (MQ=255)                                                                                                              gTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGc                                                                                                                   >  1:224047/1‑251 (MQ=255)                                                                                                                 gCGAAGGGGCAGACCCGCAACCAACAGCGCGTTTTGGGGTTTTTCAGGGGTAAAAAGAAGAGATGGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCAAAAGGCAAACAAAATAACCACGAGGGTAACAAACTCACCCCCCCATGACCCCATT‑ACCCCCCGCATCGCCTTCGGAA‑AAAGCTGAATCCCTTCCAGCGGCAGGTAGGTTGTGCCGTT‑CCCCGCCAGTAATATCAGCATGGCGCTTGCCGt                                                                                                                <  1:582244/251‑1 (MQ=255)                                                                                                                    aaGGTGCAGATCCGAAAACACAGGTTCTTTTTGGGGTTGTCCAGGCGAAAAAAGAAGAGTTTTTTTGC‑GGCTTAAATGTAGTTGGCAACGATGGAGCGGAGGGAAAACAGAATAACCCCGGGGGTACCAAACCCAGCCCCCCAGGACCCCTTT‑ACCCCCCGCAGCGCCTTCTGGA‑TAAGCTGAATCCCTCCCAGCGGCATGTAGGTTGGGCCGTT‑CCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACa                                                                                                             <  1:394866/251‑1 (MQ=255)                                                                                                                                 gCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAACAGAAGAGCTTGTTTTC‑GGCGTCAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGACTAACCACGAGGGTAACACACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGgtg                                                                                                >  2:394948/1‑251 (MQ=255)                                                                                                                                    aaCACCAGACCGTTTTAGGGTGGTTCGGGCAAAAAAAAAAGAAATTTTTTCC‑GGCGTAAATGAAGTGGGCAACGATGGAGCGAAAGGCAAAAAAAATACCCACGAGGGTAACAAACCCACCCCCCCAGGACCCCATT‑ACCCCCCGCATCCCTTTCTGGA‑AAAGCAGAATCCCTCCCAGCGGCATGTGGGTTGGGCCGTT‑CCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCg                                                                                             <  1:468409/251‑1 (MQ=255)                                                                                                                                       cccAGACCGTTTTAGGGTTGTTCGGGCAAAAAAAAAAGAGAT‑GTTTTCCGGCGAAAATGTAGTGGGAAACGAGGGAGCGGAAGGAAAACAAAATAACCACGGGGATAACAACCTCAGCACCCCAGAACCCCATT‑ACCCCCCGACCCGCCTCCGGGA‑AAACCTAAATCCCTCCCGGCGGCATGTGGGTTGTGCCGTT‑CCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATa                                                                                          <  2:230600/250‑1 (MQ=255)                                                                                                                                           gATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaaa                                                                                       >  1:519143/1‑250 (MQ=255)                                                                                                                                                    aGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatc                                                                              >  1:85980/1‑250 (MQ=255)                                                                                                                                                      ggTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc                                                                           >  1:114360/1‑251 (MQ=255)                                                                                                                                                            tCAGGCGAAAAAAGAAGAGATTGTTTGC‑GGCGAAATTGTAGTTGGCAACGAGGGAGCGGAAGGCAAACAGAATAACCACGAGGGTAACAACCTCAGCACCCCAGGAACCCTTT‑AACCCCCGCATCTCCTTCGTGA‑TAAGCTGAATCCCTCCCAGCGGCATGGGGGTTGTGCCTTC‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACTAGGGTGTCGATAAAAATGCCAATCATCTGGAc                                                                      <  1:446226/250‑1 (MQ=255)                                                                                                                                                                     aaaaGAAGCGATAGTTTTC‑GACGTAAATGTAGTTGCCAACGATGGAGCTGAAGGCACACAGAATAACCACGAGGGTAACAAACTCCGCACCCAAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGCATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACACGCCAGTACTATCAGCCTGGCGCTTGCCGTACAGATGACCAGGGGGCCGATAAAACTGCCAATCATCTGGACAATCCCTTgc                                                            >  2:319605/1‑251 (MQ=255)                                                                                                                                                                                ttGTTTTC‑GGCGTAAATGTAGTTGGCAACGCTGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATg                                                 >  2:259555/1‑251 (MQ=255)                                                                                                                                                                                ttGTTTTC‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCCGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGAGTCGATAACAATGCTAATCATCTGGACAATCCCTTTCGCTGCCGGATg                                                 >  2:221938/1‑251 (MQ=255)                                                                                                                                                                                 ttttttC‑GGCGTAAATGTAGTGGGCAAGGAGGGAGCTGAAGGCAAAAAGAATAACCACGAGGGAAACAAACTCCGCCCCCCAGGAACCCATT‑AACCCCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCGGCGGAATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATg                                                 <  1:484517/248‑1 (MQ=255)                                                                                                                                                                                       c‑GGCGTAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGCCCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGc                                          >  1:56730/1‑251 (MQ=255)                                                                                                                                                                                          gcgtAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGAAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGcc                                         >  2:541696/4‑250 (MQ=255)                                                                                                                                                                                              aaaTGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCAGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGa                                     <  1:345721/250‑1 (MQ=255)                                                                                                                                                                                                        ggaaaagagggAGCTGAAGTCAAACAAAATACCCACGGGGGTAAAAAACCCCGCACCCCGGAAACCCATT‑ACCCCCCCCCCCGCCTT‑TGGAATAAGCAGAATCCCTCCCAGGGGATTGTAGGTTG‑GCCCTTGCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACgccgctgcc                           <  2:492718/241‑1 (MQ=255)                                                                                                                                                                                                          cAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACgccgctgccgct                        >  1:157195/1‑251 (MQ=255)                                                                                                                                                                                                           aaCGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGccgctgccgctg                       >  2:168602/1‑251 (MQ=255)                                                                                                                                                                                                                   aGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGttt               >  1:597039/1‑251 (MQ=255)                                                                                                                                                                                                                   aGCTGAAGGCAAACAGAACAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACAACCGCATAGACTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGATGTACCGTT‑ACCCGCCCGTAATATCAGCAAGCCGCTTGCCGTACAGATGACCAGGGCGTCGATAAAAATGCCAATACTCTGTACAATCCCTCGCGCCGCCGGAGTAGGAGGACATGACGCCGATGCACCAGCCGCGttt               >  2:73588/1‑251 (MQ=255)                                                                                                                                                                                                                      tGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTCGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGAGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAAAGCCAATCCTCTGGAAAAACACTTGCGCTGCCGGATGCGGACGCCAGGACGCCGCTGCCGCCGCCGCGTTTGGc            >  2:484529/1‑251 (MQ=255)                                                                                                                                                                                                                       gAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCCGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATTCGGAGGCCAGGACGCCGCTGCCGCTGCCCCGTTTTGCg           >  2:343921/1‑251 (MQ=255)                                                                                                                                                                                                                        aaGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGATGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGt          >  2:1393/1‑251 (MQ=255)                                                                                                                                                                                                                                cAGAATAACCCCGAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AACACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACgccgctg                             >  2:584739/1‑224 (MQ=255)                                                                                                                                                                                                                                cAGAATAACCACGAGGGTAAAAAAATCAGCACCCCAGGCACCCATT‑AACAACCGCAGCGCCTTCTGGC‑TACGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAAAATCCGCATGGAGCTTGGCGTACAGATTACCAGGGGGTCGATAAAAATGCAAATACTCTGGACAATCAATTGCGCTGCCGGAGGAGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCgcccccc  >  2:444803/1‑245 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  AGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTC‑GGCATAAATGTAGTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATT‑AGCACCCGCATCGCCTTCTGGA‑TAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTT‑ACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGATGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCA  >  NC_000913/6664‑7136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑