Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,886	C→T	N325N (AAC→AAT)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,886	0	C	T	97.9%	275.3 / NA	96	N325N (AAC→AAT)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/53);  minor base G (0/2);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCC  >  NC_000913/4626644‑4627130                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ggCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGcgcc                                                                                                                                                                                                                                               <  1:587188/250‑1 (MQ=255)   ggATGCCGACTCCCGTTGTCGCTCTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCGGCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTTCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATccgcgccgcg                                                                                                                                                                                                                                            <  1:243070/250‑1 (MQ=255)    gATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATccgcgccgcgg                                                                                                                                                                                                                                           >  1:95944/1‑250 (MQ=255)     aTGCCGACGCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTg                                                                                                                                                                                                                                          <  1:567272/251‑1 (MQ=255)        ccGACTCCCGTTTTCGCACTTTACGCCGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGCTGGCGAATCCGCGCCGCGTGGcc                                                                                                                                                                                                                                       >  2:79769/1‑250 (MQ=255)        ccGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAGCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCa                                                                                                                                                                                                                                       <  1:125156/251‑1 (MQ=255)         cGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGc                                                                                                                                                                                                                                        >  1:124842/1‑249 (MQ=255)                     tCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGaa                                                                                                                                                                                                                           <  1:235891/250‑1 (MQ=255)                         actttacgcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACaaa                                                                                                                                                                                                                       >  2:520473/7‑250 (MQ=255)                         actttacgcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACaaa                                                                                                                                                                                                                       >  2:295407/7‑250 (MQ=255)                            tgacgcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCGGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCATAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGGGATGGTGGTGTGGGAAGGAACGCTGCCGTTGCTGGTTGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAAGCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAAc                                                                                                                                                                                                                     <  2:525601/246‑1 (MQ=255)                             tacgcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCg                                                                                                                                                                                                                   <  1:7057/248‑1 (MQ=255)                                gcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTTGAACAAAACCGTCTg                                                                                                                                                                                                               <  2:615607/251‑1 (MQ=255)                                gcAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTg                                                                                                                                                                                                               <  2:95944/251‑1 (MQ=255)                                   gCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGc                                                                                                                                                                                                             >  1:274052/1‑250 (MQ=255)                                    ccATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCa                                                                                                                                                                                                            >  1:468980/1‑250 (MQ=255)                                     cATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGCTGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAAt                                                                                                                                                                                                          >  1:590127/1‑251 (MQ=255)                                     cATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCCATTTTCTTAAGTCGCGGAGATGGAGTGAACTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGGGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAAt                                                                                                                                                                                                          >  1:558703/1‑251 (MQ=255)                                        aaaaaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCct                                                                                                                                                                                                       >  1:298548/1‑251 (MQ=255)                                         aaaaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCctg                                                                                                                                                                                                      <  1:163969/251‑1 (MQ=255)                                           aaCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCctgct                                                                                                                                                                                                    >  1:364232/1‑251 (MQ=255)                                            aCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCctgctg                                                                                                                                                                                                   <  1:221052/251‑1 (MQ=255)                                             ccGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTgctgg                                                                                                                                                                                                  >  1:502251/1‑251 (MQ=255)                                                  tCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCtgt                                                                                                                                                                                              <  1:209147/250‑1 (MQ=255)                                                   cGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCtgtct                                                                                                                                                                                            >  2:486796/1‑249 (MQ=255)                                                      cgcgGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAATCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGc                                                                                                                                                                                         <  2:93222/251‑1 (MQ=255)                                                        cgttGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCAc                                                                                                                                                                                       <  2:542246/248‑1 (MQ=255)                                                            gAATGAGCTGGGCGTCTGCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTc                                                                                                                                                                                   <  1:515653/251‑1 (MQ=255)                                                                gAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACgg                                                                                                                                                                               <  1:108912/251‑1 (MQ=255)                                                                gAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACg                                                                                                                                                                                <  2:468980/250‑1 (MQ=255)                                                                   cTGGGAGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACggtgg                                                                                                                                                                            >  1:134670/1‑251 (MQ=255)                                                                      ggCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAAGCCGCGGCGCGTGGCAGTGGGGCTGGAACAAAAGCGTCTGGCTAGCCTgctggcggtcttgttcggtcgcgtgggtct                                                                                                                                                                         <  2:38967/251‑25 (MQ=255)                                                                       gCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCCACCCTTCGGCGATTTTCTTAAGTCGCGGAGATGAATTGACCTCCGGCCGCTCTGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACACAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACggcggcctg                                                                                                                                                                        >  1:296880/1‑244 (MQ=255)                                                                              cgcgATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTGCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCATCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTTGGGCGGGAACAAAACCGTCTGGCAATCCTGCGGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATg                                                                                                                                                                  <  2:575637/250‑1 (MQ=255)                                                                                    gACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGACTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATc                                                                                                                                                           >  1:277706/1‑251 (MQ=255)                                                                                    gACCGAACAGGGGCTGCGGGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGTCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGAt                                                                                                                                                            <  1:576685/250‑1 (MQ=255)                                                                                           cAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTGAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGAtgt                                                                                                                                                    <  2:166511/251‑1 (MQ=255)                                                                                            aGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGAtgtg                                                                                                                                                   >  1:305159/1‑251 (MQ=255)                                                                                            aGGGGCTGCGTGAAGTCAGCAACCCTTCGGAAATTTTCTTAAGTCGAGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCAGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGAtg                                                                                                                                                     >  2:559294/1‑249 (MQ=255)                                                                                             ggggCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGCACGCGGCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGCGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGGCTTGCCCCGTCACGGGGGTCTGCAAATGGCCGATCAGGAtgagg                                                                                                                                                  >  2:4575/1‑248 (MQ=255)                                                                                             ggggCTGCGTGAAGTAAGCAACCCTTCGGAAATTTTCTTAAGTCGCGGAGATGAAGTGACCCCCGGCCGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGACGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGAGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGACGATCAGGATgtgt                                                                                                                                                  >  2:356892/1‑251 (MQ=255)                                                                                                gCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGGCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGCTGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCCAATGGCCGATCAGGCTGTGtttt                                                                                                                                               >  1:250875/1‑250 (MQ=255)                                                                                                   gCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTtgtg                                                                                                                                             <  1:486796/250‑1 (MQ=255)                                                                                                    cGTGAAGTCAGCAATCCTTCGGCAATTTTCTTAAGGAGCGGAGATGAAGTGACCGCCGGCAGCTCCGTGAGGGTGGTGGGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGaa                                                                                                                                           <  1:146424/251‑1 (MQ=255)                                                                                                        aatgcAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTg                                                                                                                                       <  2:127974/247‑1 (MQ=255)                                                                                                                  cccTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGgcggcg                                                                                                                             >  1:165790/1‑251 (MQ=255)                                                                                                                  cccTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGGGAACGGGGTCGgcggcg                                                                                                                             >  1:141703/1‑251 (MQ=255)                                                                                                                        ggCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGGGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAgg                                                                                                                       <  1:484471/251‑1 (MQ=255)                                                                                                                        ggCAATTTTCTTAAGTCGCGGAGAGGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAgg                                                                                                                       <  2:539972/251‑1 (MQ=255)                                                                                                                        ggCAATTTTCTTAAGCCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTACGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGCTGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAACTGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAgg                                                                                                                       >  2:321767/1‑251 (MQ=255)                                                                                                                               ttCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGGGGTCGGCGGCGTGAAGGTAACCg                                                                                                                 <  2:377264/250‑1 (MQ=255)                                                                                                                                tCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGCACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGa                                                                                                                >  1:80690/1‑250 (MQ=255)                                                                                                                                     aGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCCCCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTTTGACCTAGGTAGGCGGCGTGACGGGACCCGAAAcc                                                                                                            >  2:481648/1‑249 (MQ=255)                                                                                                                                     aGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGGAACCGAAAcc                                                                                                            >  1:338919/1‑249 (MQ=255)                                                                                                                                      gTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGGGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCTATCCTGCTGGCTGTCTTGCACCGTCTCGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAgc                                                                                                         <  1:41984/251‑2 (MQ=255)                                                                                                                                        cgcgGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAgcgc                                                                                                       >  2:323810/1‑248 (MQ=255)                                                                                                                                            gggaggAAGTGACCTCCGGCAGCGCCGTGATGGTGGTGTGGGAAGGAACGCGGCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACGCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGAc                                                                                                   <  2:124842/246‑1 (MQ=255)                                                                                                                                            gagATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGGGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGAc                                                                                                   <  1:520473/249‑1 (MQ=255)                                                                                                                                              gATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACtt                                                                                                 <  2:134670/251‑1 (MQ=255)                                                                                                                                              gATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGGGAGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACtt                                                                                                 >  1:19933/1‑251 (MQ=255)                                                                                                                                                    gTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGtt                                                                                           <  1:79769/251‑1 (MQ=255)                                                                                                                                                    gTGACCGCCGGCAGCTCCGGGAGGGTGGGGTGGGAAGGCACGCGTCCTTTGCTGGTGGAGATTCAGGCGCGGGTCGATCACTCGATGAGGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTGTGTGAACGTGGGCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGtt                                                                                           <  2:67722/251‑1 (MQ=255)                                                                                                                                                      gcccgccGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGGCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTa                                                                                          <  2:116143/245‑1 (MQ=255)                                                                                                                                                           ccggcagtgacgggATGGTGGGGTGGGAAGGAACGCGTGCGTTGCTGGTGGAGATTCGGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGACCAGGATGTGTTTGTGAACGGGGGCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTActgc                                                                                      <  2:250875/236‑1 (MQ=255)                                                                                                                                                           ccGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTGGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTActgctg                                                                                    <  1:559294/251‑1 (MQ=255)                                                                                                                                                             ggcagctacgTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGACGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGACTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGCGGGCTGCAAAATGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGCCGTGAAGTTAACCGACACCAGCGCCGACTTAGCGTTACTGCTGGc                                                                                  >  2:614623/10‑251 (MQ=255)                                                                                                                                                               caGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGCGATCAAGGCGCTGGTCGATCACTCGCTGCTGGCGAATCCGCGCCGCGTGGCAGTGCGGCAGGAACCAAAACATCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGCTCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAACCCAGCGCCGACTTAGCGTTACTGCTGGCg                                                                                 >  1:369158/2‑250 (MQ=255)                                                                                                                                                               caGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCg                                                                                 >  1:377975/2‑250 (MQ=255)                                                                                                                                                                        taTGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTGGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTcc                                                                        <  2:427706/249‑1 (MQ=255)                                                                                                                                                                        gATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCa                                                                       <  1:11480/251‑1 (MQ=255)                                                                                                                                                                        gATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGAGGGTTTCCa                                                                       <  2:107908/251‑1 (MQ=255)                                                                                                                                                                         aTGGTGGTGTGGGAAGGATCGCGTCGGTTGCTGGTGGCGATGCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGGGGCAGTGGGGCTGGAACAAAACCTTCGGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACGTAGCGTTACTGCTGGCGATGGTTTCCAg                                                                      <  2:5273/251‑1 (MQ=255)                                                                                                                                                                           ggtggtGTGGGAAGGAACGCGCCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGAGGGGCTGGAACAAAACCGTATGGCCATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCACATGGCCGATCAGGATGTGGTTGCGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGAGCCGACTTGGCGTTTCTGCTGGCGATGGTTTCCaacc                                                                    >  2:37388/1‑248 (MQ=255)                                                                                                                                                                              ggtgtGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCTCTGGTCGATCACGCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGGCTTGCACCGTCACGGGGGTCTGCAAATTGCCGATCAGGATGTGTTTGTGAACGTGGGCGGCGGCGTGAAGGTAGCCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTgc                                                                 <  1:325285/247‑1 (MQ=255)                                                                                                                                                                               gtttgtgaaggacCGCGTCCGTTGCTGGGGGAGAGATAGTCGCTGGTCGAGCACTCGATGCTGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCGGCTGGCGGTCTTGCACCGTCGCGGTGGGCTGCAAATGGCCGATCTGGATGTGTGTGTGAGCGTGGTCGGCGGCGTGAAGGGAACGGAGCCCAGCGCCGACTTAGCGTTACTGCTTGCGAGGGCTTCCATCCTgcg                                                                <  2:545188/245‑1 (MQ=255)                                                                                                                                                                                tgtGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTG‑AACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTgcgtg                                                              >  2:270827/3‑249 (MQ=255)                                                                                                                                                                                 gtgggaaggagcgggtccgtggggggtggAGATTCAGGCGCTTGTCTATCACTCGATGATGGCGAATCCGCGTCGCGGTGCAGTGTGGCTGGAACAAATCCGTCTGGCAATCCTGCTGGCTGTCGTGCTCCGGCACGGTGGGCTGCAAAGGGCCGATCGGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTCACCGAATCCAGCGCCGACTTAGCGGTTCGGCTGGCGAGGGTTTCCAGCCTgcgt                                                               <  2:528169/226‑2 (MQ=255)                                                                                                                                                                                   gggAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGAc                                                            <  1:541860/251‑1 (MQ=255)                                                                                                                                                                                       aggaaCGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGAc                                                        <  1:295177/251‑1 (MQ=255)                                                                                                                                                                                        ggaaCGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGAcc                                                       <  2:67028/251‑1 (MQ=255)                                                                                                                                                                                           aCGCGTCCGTTGCTGGTGGGGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCt                                                    <  2:64639/251‑1 (MQ=255)                                                                                                                                                                                               gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGcc                                                 >  1:12899/7‑250 (MQ=255)                                                                                                                                                                                                   gttgttgggggAGAGTCAGGCGCTGGTAGATCACGCGAGGATGGCGAATCCGCTCCGCGTGGCAGTGTGGCTGCAACAAAACCGTCTGGCAATCCTGCGGGCTGTCTTGCACCGTCACGGTGGTCTGCAAAGGGCCGATCAGGATGGGTTTGTGAACGTGGGCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACGTAGCGTTACTGCTGGCGATGGTTTCCAGCCGGCGTGACAGACCGCTGCCACAgg                                            <  2:236166/242‑1 (MQ=255)                                                                                                                                                                                                     tgctgGTGGATATGCAGGCGCTGGTCGATCTCTCGAGGATGGGGAATTCGCGCGGCGTGGCTGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGCCTTGGACCGTAACGGTGGTCTGCAAATGGCCGATCAGGATGTTTTTGTGAACGGTGTCGGCGTCGTGAAGGTAACCGAAACCAGCGCCGGCTTAGCGTTACTGCTGGCGATGGTTTCCAGACTGGGTGACGGACCGCTGCCTCAGGa                                           <  2:296880/250‑1 (MQ=255)                                                                                                                                                                                                      gctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATc                                         <  1:358132/251‑1 (MQ=255)                                                                                                                                                                                                          gtggAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACTAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGATACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCtggt                                     >  1:396074/1‑251 (MQ=255)                                                                                                                                                                                                             gagaTTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTggtggt                                  >  2:110846/1‑251 (MQ=255)                                                                                                                                                                                                               gaTTCAGGCGCTGGTCGATCCCTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGgtgt                                <  2:260382/251‑1 (MQ=255)                                                                                                                                                                                                                aTTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTGGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGtt                               <  1:481648/251‑1 (MQ=255)                                                                                                                                                                                                                    gggCGCTGGTCGATCACTCGATGAGGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCGGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGt                           <  2:298548/250‑1 (MQ=255)                                                                                                                                                                                                                          tGGTCGATCACTCGCTGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTc                     >  1:487516/1‑251 (MQ=255)                                                                                                                                                                                                                           ggTCGTTCACTCGATGAGGTCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAATCCGTCTGGCAAGCCTGCTGGCTGTCTTGCACCGTCACGGGGGTCTGCAAATGGCCGATCAGGATGTGTTTGGGAACGTGGTCGGCGGCGTGAAGGTAGCCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGt                      <  2:192886/249‑1 (MQ=255)                                                                                                                                                                                                                                aTCACTCGATGATGGCGAATCCGCGCCGCGAGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTg               >  1:473823/1‑251 (MQ=255)                                                                                                                                                                                                                                          gatgGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGACCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAgggg        >  1:450746/1‑248 (MQ=255)                                                                                                                                                                                                                                           atgGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGGGAAGGTAACCGAAACCAGCGCAGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGGGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGGGAAGTCGGGCGGGCAGGGgag      >  2:541907/1‑249 (MQ=255)                                                                                                                                                                                                                                             ggCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATcc  <  1:350882/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCC  >  NC_000913/4626644‑4627130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑