Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,568	G→A	G219G (GGG→GGA)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,568	0	G	A	97.9%	269.8 / NA	94	G219G (GGG→GGA)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (45/46);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAA‑GATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTT‑G‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGT  >  NC_000913/4626321‑4626810                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gggCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTAGAGTCAGCGTCTGGCAGCGTGGCGCATGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCATAACGCGCGGTGTGGCGATTGGCATGGTGGGacac                                                                                                                                                                                                                                                    <  2:298251/250‑1 (MQ=255)              tCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGTGGCGATTGTCATGGTTGGACCCGTAACCAAA‑GATg                                                                                                                                                                                                                                      >  1:67722/1‑251 (MQ=255)               cATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATg                                                                                                                                                                                                                                      <  2:200223/250‑1 (MQ=255)               cATCGCCTTGGCCTGCCAACTGACAATCTCAATATGTTGGCGGAAAACAGCATCGAGCAGAACTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCAGATGTACAGGCACCGCCTGGCGGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTTGACACGTAACCAAA‑GATg                                                                                                                                                                                                                                      <  1:335230/250‑1 (MQ=255)                aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACCGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAACCGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGGGGGGCGTTTGGCATGGGTGGACACGGAACCAAA‑GATg                                                                                                                                                                                                                                      >  2:499808/1‑249 (MQ=255)                aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATggc                                                                                                                                                                                                                                    >  1:421831/1‑250 (MQ=255)                aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATggc                                                                                                                                                                                                                                    <  2:502082/251‑2 (MQ=255)                  cGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATggct                                                                                                                                                                                                                                   <  1:274012/250‑3 (MQ=255)                       tGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCgctgg                                                                                                                                                                                                                             >  1:291995/1‑251 (MQ=255)                       tGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCgctg                                                                                                                                                                                                                              <  2:449376/250‑1 (MQ=255)                                 aCTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGcccta                                                                                                                                                                                                                    >  1:522969/1‑248 (MQ=255)                                   tGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGGGATGCATATGGCGGGTGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGACACGGAGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGAAACGTAAACAAA‑GATGGCTCGCTGGCGGGccctaa                                                                                                                                                                                                                   >  1:60570/1‑246 (MQ=255)                                    gACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTaaa                                                                                                                                                                                                                  <  2:97332/249‑1 (MQ=255)                                              atatGTTTTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGTTCGCTGGCTGGCCCTAAAGTGCTGGAacac                                                                                                                                                                                                      <  1:554812/251‑1 (MQ=255)                                              atatGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAacac                                                                                                                                                                                                      <  2:178297/251‑1 (MQ=255)                                                atGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGGTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTg                                                                                                                                                                                                    >  1:146210/1‑251 (MQ=255)                                                atGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGAGTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTGATCTGACTCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAGAGTGCTGGATCACTg                                                                                                                                                                                                    <  2:378860/251‑1 (MQ=255)                                                    tgtCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGGTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGGAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTAt                                                                                                                                                                                                 <  1:618915/250‑1 (MQ=255)                                                     gtCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATc                                                                                                                                                                                                <  1:150952/250‑1 (MQ=255)                                                       cGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGAc                                                                                                                                                                                             <  1:508084/251‑1 (MQ=255)                                                         gAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACACTGTATCGACTg                                                                                                                                                                                           >  1:487397/1‑251 (MQ=255)                                                             ccAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAC‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCg                                                                                                                                                                                       >  1:575637/1‑251 (MQ=255)                                                                 cATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGc                                                                                                                                                                                   >  1:612404/1‑251 (MQ=255)                                                                    cgagcAGATCTGCCTGATTGCCTAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATGGTCATGGTTGGACACGTAACCAAA‑TATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCtt                                                                                                                                                                                 <  1:30371/246‑1 (MQ=255)                                                                    cgagcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCttt                                                                                                                                                                                >  1:615607/5‑251 (MQ=255)                                                                    cgagcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCttt                                                                                                                                                                                <  2:177545/247‑1 (MQ=255)                                                                    cgagcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCtt                                                                                                                                                                                 <  2:310698/246‑1 (MQ=255)                                                                      atcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGAGGGTAATTGACTCGATCCAGGTGATGCATATGGCTGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGGAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTAGCGACTGTTCTGTGCTTTTg                                                                                                                                                                              <  1:210358/249‑1 (MQ=255)                                                                        cAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCTTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGa                                                                                                                                                                            <  1:144490/251‑1 (MQ=255)                                                                             cTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCg                                                                                                                                                                       >  1:109813/1‑251 (MQ=255)                                                                               gCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGAt                                                                                                                                                                     >  1:427706/1‑251 (MQ=255)                                                                                 cTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGGTTGTCATGGTTGGACCCGTAACCAAA‑GAGGGCTCGCTGGCGGGCCCTAAAGGGGTGGAACACTGAATCGACTGTTTGTGGCTTTTGGGTgggggtgc                                                                                                                                                                   >  2:346211/1‑245 (MQ=255)                                                                                   gATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACGCGATCCAGGTGATGCATATGGCTGATGTACAGTCAGCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGcc                                                                                                                                                                  <  2:289862/250‑1 (MQ=255)                                                                                     ttGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGAc                                                                                                                                                               >  1:127974/1‑251 (MQ=255)                                                                                                   cAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGAAGCGTGGCGCAGGTGAGTGAAACGGCGGGTTATTTGACGCGCTTCGCCAAAAAGCGGGGTGTGTCGGTTGTCATGGTTGTACACGGAACCAAA‑GATGTCTCGCTGGCGGGGCCCAAAATGGTGGAAAACTGTATCTACTGTTCGGGGGTTTTGGATGGCGAAGTGGAATCCCGTTTTCTCAc                                                                                                                                                 >  2:337828/1‑251 (MQ=255)                                                                                                     aCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTTGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGccctt                                                                                                                                               >  2:235891/1‑247 (MQ=255)                                                                                                      ccGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCACAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGAATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCActtt                                                                                                                                              >  1:418419/1‑248 (MQ=255)                                                                                                      ccGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGACCTAAAGCGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGCCTCCCGTTTTCTCActtt                                                                                                                                              >  1:354240/1‑248 (MQ=255)                                                                                                       cGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTTTCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCGCActttt                                                                                                                                             >  1:116143/1‑247 (MQ=255)                                                                                                           gCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCa                                                                                                                                       <  1:170639/251‑1 (MQ=255)                                                                                                                 ggTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATa                                                                                                                                 <  2:485417/251‑1 (MQ=255)                                                                                                                       tGACTCGATCCAGGTGATGCATTTGGCGTATGTACGGGCATCGCCTGGCAGCGTGGCGCAGGGGCGTGAAACGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GAGGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAAc                                                                                                                            <  2:500164/250‑1 (MQ=255)                                                                                                                        gACTCGCTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGGCACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑C‑CGCAACCATAAAAAc                                                                                                                            >  2:122025/1‑249 (MQ=255)                                                                                                                        gACTCGATCCAGGTGATGCATATGGCGGCTGTACAGTCATCGCCCGGCAGCGTGGCGCAGGGGCGTGAAACGGCGGCTTAACTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAATGCTGGGACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACGCCCGTTTTCGCACTTT‑A‑CGCCGCCATAAAAACCg                                                                                                                          >  2:398064/1‑251 (MQ=255)                                                                                                                        gACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑T‑CGCAGCCATAAAAACCg                                                                                                                          >  1:107908/1‑251 (MQ=255)                                                                                                                            cGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTc                                                                                                                      <  2:487397/251‑1 (MQ=255)                                                                                                                             gATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGAAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCCCGCTGGCGGGCCCTACAGTGCTGGGACACTGAATCGACTGTTCGGTGCTTTTGGGTGGCGATGCCGACTCCAGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACAGCTTCg                                                                                                                     >  1:437094/1‑251 (MQ=255)                                                                                                                              aTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCgg                                                                                                                    <  2:99810/251‑1 (MQ=255)                                                                                                                                ccAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCCAAACGCGCGGTGTGGCGATTGTCATGGTTGGGCACGTAACCAAAAGA‑GGCTCGCTGGCGGGCCCTAAAGAGCTGGGACACTTTATCGGATGTTTGGGGGTTTTGGATGGGGCTGCCGGCCCCCGTTTTTACACTTT‑T‑CGTAGCCATAAAAACCCCTTCGgcg                                                                                                                  >  2:237617/1‑251 (MQ=255)                                                                                                                                  aGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGGATGTTCGGTGATTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCCTAAAAACCGCTTCGGcgcg                                                                                                                >  2:417225/1‑251 (MQ=255)                                                                                                                                  aGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGGTGGCGATGCCGCCTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGcgcg                                                                                                                >  1:211699/1‑251 (MQ=255)                                                                                                                                  aGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGcgcg                                                                                                                <  2:417620/251‑1 (MQ=255)                                                                                                                                  aGGTGATGCATAGGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACTTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGcgcg                                                                                                                <  1:393526/251‑1 (MQ=255)                                                                                                                                   ggTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGGCTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCgg                                                                                                               >  1:539972/1‑251 (MQ=255)                                                                                                                                   ggTGATGCATATGGCGGATGTACAGTCATCGCCCGGCAGCGGGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGGAACCAAA‑GATGGCTCGCTGGCGGGCCCTCAAATGCTGGAACACTGTATCGACCGTTCGGTGCTTTTGGATGGCGATGCCCACTCCCGTTTTCGCCCTTT‑A‑AGCAGCCATAAAAACCGCCTCGGCGCgg                                                                                                               >  2:567272/1‑251 (MQ=255)                                                                                                                                   ggTGATGCATATGGCGGATGTACAGTCAGCGCCTGGCAGCGTGGCGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGGTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAACCCGCTTCGGCGCgg                                                                                                               <  2:306166/251‑1 (MQ=255)                                                                                                                                        tGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTGACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACACTGTATCAACCGTTCGGGGGTTTTGGATGGGGATGCCGACCCCCGGTTTAGCCCTTT‑A‑CGCAGCCATAAAAACCGccttggcgggggggat                                                                                                          >  2:358132/1‑236 (MQ=255)                                                                                                                                           atatGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCCCGCTGGCGGGCCCTAAAGGGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑T‑CGCAGCCATAAAAACCCCCTCGGCGCgggtattg                                                                                                         >  2:11480/1‑243 (MQ=255)                                                                                                                                            tatGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGGTTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGGTGGc                                                                                                                                                                        >  1:275226/1‑187 (MQ=255)                                                                                                                                            tatGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGc                                                                                                                                                                        <  2:275226/187‑1 (MQ=255)                                                                                                                                             atGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTATAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                                     <  1:613454/251‑1 (MQ=255)                                                                                                                                             atGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGGGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                                     <  1:255616/251‑1 (MQ=255)                                                                                                                                             atGGCGGATGTACAGTCATCGCCGGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCt                                                                                                     <  1:339311/251‑1 (MQ=255)                                                                                                                                              tGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACCCTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATCAAAACCGCTTCGGCGCGGTGAATGGGCTg                                                                                                    >  1:512365/1‑251 (MQ=255)                                                                                                                                               ggCGGATGTACAGTTTTCGCCTGGCAGCGGGGCGCAGGTGCGTGAAACGGCTGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGTCATAAAAACCGCTTCGGCGCGGTGAATGAGCTgg                                                                                                   <  2:81184/251‑1 (MQ=255)                                                                                                                                               ggCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTgg                                                                                                   <  1:29448/251‑1 (MQ=255)                                                                                                                                                      gcaCAGTGATCGCCTGGCAACGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGGTGTGGAGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACCCCCGTTTTCGCACTTT‑T‑AGCAGCCCTAAAAACCGCTACGGCGCGGTGAAGGAGCTGGGctgctt                                                                                            >  2:100038/3‑246 (MQ=255)                                                                                                                                                        aCAGTCATCGCCTGGCAGCGTGGCGCAGGGGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTACCCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCAC‑TT‑GACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg                                                                                          <  2:460847/251‑1 (MQ=255)                                                                                                                                                          aGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcgcg                                                                                        >  1:616792/1‑251 (MQ=255)                                                                                                                                                                     ggcaGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAc                                                                              >  1:542246/4‑250 (MQ=255)                                                                                                                                                                       caGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGCCTGTTCGGGGCTTTTGGATGGCGCTGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCGTCGGCGCGGTGAATGAGATGGGCGTCTTCGCGCTTACCGAACAgg                                                                           >  2:461587/2‑251 (MQ=255)                                                                                                                                                                               cgcAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAAAGCGCGGTGTGGCGATTGTCATGGTTGGCCACGTAACCAAA‑GATGGCTCGCTTGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCG‑ACCTTTA‑CCCAGCCATAAAAACCGCTTCGGCGCGGTGAAAGAGCTGGGCGTCTTCGGGATGACCGAAAAGGGGCTGCgg                                                                   >  2:459261/1‑250 (MQ=255)                                                                                                                                                                                 catgTGCGTGAAACGGCGGCTTATCTTACGTGCTTCCCCAAAGCGCGCGGTGTGGCGATTATCATGGTTGGACACGTAACCAAA‑GAGGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACTCTGTATCTACTATCCGGTGCTTTTGGATGGCGATTCCTACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGGTGGACGTCGTCGCGATGACCGAACAGTCGCTGCGTGa                                                                 <  2:437094/248‑1 (MQ=255)                                                                                                                                                                                           aaaCGGCGGCTTATCTGACGCGCTGCGCCAAAACGCGCGGTGGGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTAGCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAc                                                       <  2:376488/251‑1 (MQ=255)                                                                                                                                                                                            aaCGGCGGCTTATCTGACTCGCTGCGCCAAAACTCGCGGTTTGGCGATTGTCATGGTTGTACACGTAACCAAA‑GATGGCGCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGCTGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAcc                                                      <  2:226033/251‑1 (MQ=255)                                                                                                                                                                                                  ggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCgg                                                >  1:384892/1‑251 (MQ=255)                                                                                                                                                                                                  ggcTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCgg                                                >  1:260382/1‑251 (MQ=255)                                                                                                                                                                                                    gttatctggcgcgcatggCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGATACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATAGCGATGCCGACTCCCGTTTTCGCAC‑TT‑GACGCAGCCATAAAAACCGCGTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCgtca                                              <  2:430827/234‑4 (MQ=255)                                                                                                                                                                                                     ttatctgaagagCTTCGCCAAAGCGCGCGGTGTGGCGCTTGTACTGGTTGGACCCGTACCCACA‑GATGGCTCGCTGGCGGGAACTAACGGGCTGGAACAGTGTATCAACTGTTAGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAAACGCTTCGGCGAGGTTCCTGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCGGCAACCCTTCGGCa                                              >  2:576685/12‑250 (MQ=255)                                                                                                                                                                                                       atctGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCCCGATGACCGAACAGGGGCTGCGTGAAGTCAGCACCCCTTCGGCAAtt                                           >  2:185011/4‑251 (MQ=255)                                                                                                                                                                                                            acgcGCTTCGCCAAACCGCGCGGTGTGGCGATTGTCATGGTTGGACACGTACCCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCtt                                      <  2:295831/248‑1 (MQ=255)                                                                                                                                                                                                                gCTTCGCCAAAACGGGCGGTGTGGCGAGTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACAGTGTGTCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGt                                  <  2:532185/251‑1 (MQ=255)                                                                                                                                                                                                                gCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGt                                  >  1:192886/1‑251 (MQ=255)                                                                                                                                                                                                                ccTTCGCCAAAGCGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAT‑GATGGCTCTCTGGCGGGCCCTAATGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGAGGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAATCCTTCGGCAATTTTCTTAAGt                                  <  2:569101/250‑1 (MQ=255)                                                                                                                                                                                                                    cGCCAAAACGCTCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTcgc                               <  2:421831/250‑1 (MQ=255)                                                                                                                                                                                                                        aaaaCGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAAAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCgcgg                             >  1:579741/1‑248 (MQ=255)                                                                                                                                                                                                                          aaCGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGTAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATg                        <  1:237617/251‑1 (MQ=255)                                                                                                                                                                                                                             gcgcgGTTTTGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAg                     <  1:69973/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggTGTGGCGATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGAc                 >  2:295177/1‑251 (MQ=255)                                                                                                                                                                                                                                         gATTGTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggcc         >  2:541860/1‑249 (MQ=255)                                                                                                                                                                                                                                          atggTCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggcag        <  1:499808/248‑4 (MQ=255)                                                                                                                                                                                                                                              tCATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTcc    >  1:284588/1‑250 (MQ=255)                                                                                                                                                                                                                                               cATGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTccg   <  2:522969/251‑3 (MQ=255)                                                                                                                                                                                                                                               cATGGTTGGACACGTAACCAAA‑GATGGCTCGCGGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTcc    <  2:109813/250‑2 (MQ=255)                                                                                                                                                                                                                                                aTGGTTGGACACGTAACCAAA‑GATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTT‑A‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTccgt  >  1:64639/1‑248 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAA‑GATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTT‑G‑CGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGT  >  NC_000913/4626321‑4626810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑