Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969	T→G	A211A (GCT→GCG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969	0	T	G	100.0%	139.6 / NA	45	A211A (GCT→GCG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/13);  total (32/13)

TCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACC  >  NC_000913/724‑1208                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tCGATCGCCCTTCTGGCCGGCGTATTAGAAGCGCGCGTTCACAACGTTACCGTAATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAACAAGGCGACCTGGTGGTACTTGGCCGCAACGGTTCCGACTACTCCGcggcggg                                                                                                                                                                                                                                             >  2:323895/1‑250 (MQ=255) tCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGcggcggg                                                                                                                                                                                                                                             >  1:463793/1‑250 (MQ=255) tCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGt                                                                                                                                                                                                                                             >  2:198302/1‑251 (MQ=255)   gATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTgc                                                                                                                                                                                                                                           >  1:129594/1‑251 (MQ=255)               ggCCGGCGTATTAGAAGCGCGCGGTCACAAAGTTACCGTTATCGATCCGGACGAAAAACTGCTGGCCGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGCCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGCTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGtt                                                                                                                                                                                                                               >  2:258859/1‑251 (MQ=255)                 ccGGCGTGTTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGCATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGAATTCCGGCTGATCACATGGTGCTGATGGCCGGTTTCACAGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCGGCCAGtttc                                                                                                                                                                                                                             >  2:227933/1‑250 (MQ=255)                       tATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGcc                                                                                                                                                                                                                       >  1:596700/1‑251 (MQ=255)                              aGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGCAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGAttgtt                                                                                                                                                                                                                >  2:263388/1‑251 (MQ=255)                                cgcgcgGTCACAACGTTACCGTTATCGATCAGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGCACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCCGGCTGTTTACGCGCCGATTGTTGc                                                                                                                                                                                                              >  1:198867/1‑251 (MQ=255)                                                    gTTATCGATCCTGTCGAAAAACTGCTGGCAGTGGGGCATTCCCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCCGGTTTCACCGACGGTAATGAAAAAGGCGAACTGGTGGCAATTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTCCGCGCAGATTGCTGCGAGATTTGGCCGGGCGTTgg                                                                                                                                                                                          >  2:627216/1‑250 (MQ=255)                                                                  cGAACAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCCCCGCCGGTAATGAAAAAGGCGAACTGGTGGCACTTGGACGCAACGGTTCCGACTAATCCGCGGCGGTGCTGGCTGGCTGTTTATGCGCCGGTTGTTGCGAGGTTTggacgggagttttcggggtctttcacc                                                                                                                                                                            >  1:595954/1‑230 (MQ=255)                                                                    aaaaaCTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGACAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGTGCCTGTTTACGCGCCCGTTGTTGCGAGGTTTGGACGGACGTTGACGGGGTCTCTACCTGc                                                                                                                                                                          >  1:337812/1‑251 (MQ=255)                                                                          tgctgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCcg                                                                                                                                                                    >  1:381568/1‑251 (MQ=255)                                                                           gctgGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCcgc                                                                                                                                                                   >  1:439628/1‑251 (MQ=255)                                                                                     gggCATTACCTCGAATCTACCGTCGATATTGCTGCGTCCACCCGCCGAATTGCGGCAAGTCGCATTCCGGCAGATAGCCAGGCACTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGTACTGGGGGTACTTGGACGCACCGGTTCCGACTACTCCGCGGCGGTGCTGGCAGCCTGTTTAAGCGCCGATTGATGAGAGATTTGGACGGACGTTGAACGGGTCtataacgtcggcccgggacatggacc                                                                                                                                                         >  1:425323/1‑229 (MQ=255)                                                                                       gCATTACCTCGAATCTACCGTCGATATTGCTGAGTACACCCGCCGAATTGCGGCAAGTCGCATTCCGGCTGATCAAATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGAGGCGGTGCTGGATGCCTGTTTACGCGCCGATTGTTGCGGGCTTTGGACGGACGTTGACGGGGCCTATACCCTCGACCCGCGTCAGGTGGCCg                                                                                                                                                       >  1:262244/1‑251 (MQ=255)                                                                                                      caccctctttttgggtatctccccccGCCGTATTGCTGCAAGTCGCATTCCGGCGTATCACATGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGa                                                                                                                                        <  1:143448/228‑1 (MQ=255)                                                                                                         cGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCCGCCTGTTTTCGCGCCGGTTGTTGCGAGATTTGGACGGACGTTGACGGGGGCTATACCTGCGGCCCGCGGCAGGGGCCCGATGCGGGGttgttggagg                                                                                                                                     >  2:542522/1‑247 (MQ=255)                                                                                                             gATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGACGGTAATGAAAAAGGCGAACTGGTGGTCCTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCCGCGACCCGCGTCAGGAGCCCGATGCGAGGGTGTTGAAggcggt                                                                                                                                 >  1:412174/1‑246 (MQ=255)                                                                                                                         tCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGGTGCCTGTTTAAGCGCCGATTGTTGCGAGATTTGGACGGGCGGTGACGGGGTCTATACCTGCGAACCGCGTCAGGGGCCCGATGCGAGGTTGTTGGAGTCGGTGTCCTAcccgg                                                                                                                      >  2:446314/1‑247 (MQ=255)                                                                                                                         tCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGAGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGCCTATACCTGCGCCCCCCGTCAGGTGCCCGGTGCGAGGGTGTTGAAGGCGATGGCCTACCAggg                                                                                                                     >  2:345777/1‑250 (MQ=255)                                                                                                                          ccACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGa                                                                                                                     >  1:401480/1‑250 (MQ=255)                                                                                                                              ccgccgTATTGCGGCAAGTCGAATCCCGGCGGTTCACATGGTGCTGAGGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGa                                                                                                                <  1:585065/251‑1 (MQ=255)                                                                                                                               cgccgTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCCGGTGCCCGATGCGCGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGAt                                                                                                               >  1:60219/1‑251 (MQ=255)                                                                                                                                gccgTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGGGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGCGGTTGTTGAAGTCGGTGTCCTACCAGGAAGCggtg                                                                                                              >  2:353802/1‑248 (MQ=255)                                                                                                                                             agggcGCATTCCGGTTGACCCCATGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACt                                                                                                 <  1:353802/247‑1 (MQ=255)                                                                                                                                                          gCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGTAGCTTTCCTACTTTGGCGCTAAAGt                                                                                    >  1:400791/1‑251 (MQ=255)                                                                                                                                                                   aTGGTGCTGATGGCAGGTTTCACAGCCGGTAATGCAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGGCTTTACCTGCGACCCGCGTCAGGGGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCAccc                                                                           >  1:172222/1‑251 (MQ=255)                                                                                                                                                                        gtggaggcagtgttcacccgccgGTAA‑GAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCAc                                                                     <  1:437246/234‑1 (MQ=255)                                                                                                                                                                        gCTGATGGCAGGTTTCACCGCCGGTAATGACAAAGGCGAACTGGTCGCAATTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCAGTTTACGCGCCCATTGTTGCGAGCTTTGGAAGGACGTTGACGGGGTCTATAACTGCGACCCGCGTCAGGGGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTCCTTCACACCCGcc                                                                      >  1:266992/1‑250 (MQ=255)                                                                                                                                                                         aggaggtcagttttCCCCGACGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTGTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGGCCTACCATGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTGTTCAcctgcgcac                                                                     <  2:288047/240‑8 (MQ=255)                                                                                                                                                                              ggCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCACCGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGGGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCAtt                                                                 >  1:50983/1‑250 (MQ=255)                                                                                                                                                                               gggggTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAc                                                               <  1:263388/248‑1 (MQ=255)                                                                                                                                                                               gCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCCCCCCATTAc                                                               >  2:442888/1‑251 (MQ=255)                                                                                                                                                                                 aggcttCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGCCGCAACGGTTCCGACTACTCCGCGGCGGTGGTGGCTGCCTGTTTACGCGCCGCTTGTTGCGAGATTTGGACGGACGTTGAAGGGGTCTATACCTGCGACCCGCGGCAGGTGCCCGGTGCGAGGTTGCTGAAGTCGGTGTCCTACCAAGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGCTCTTCACCCCCGCACCATTAccc                                                             >  1:603549/5‑251 (MQ=255)                                                                                                                                                                                 acgTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGGTTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGGGGTTGTTGAAGTCGATGTCCTACCCGGAAACGATGGGGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCCCACCCTTAccc                                                             >  2:340212/3‑251 (MQ=255)                                                                                                                                                                                            ccgGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAg                                                 <  1:442888/251‑1 (MQ=255)                                                                                                                                                                                                 aaTGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTTCTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGCTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTcc                                             <  2:156689/250‑1 (MQ=255)                                                                                                                                                                                                     aaaaaGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATc                                        <  1:446314/251‑1 (MQ=255)                                                                                                                                                                                                     aaaaaGGCGAACTGGGGGTACTTGGACGCATCGGTTCGGACTACGCCGCGGTGGTGCTGGCTGCCTGTTGACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCTCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCAGTACCCCCCATCGCCCAGTTCCAGAt                                         <  2:523213/251‑1 (MQ=255)                                                                                                                                                                                                                          ttGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTCCGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGACTCTACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGCAGCCATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCCGTTCCAGATCCCTTGCCTGATTAAAAATAcc                   >  1:420413/1‑251 (MQ=255)                                                                                                                                                                                                                           tGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATAcc                   <  2:596700/250‑1 (MQ=255)                                                                                                                                                                                                                             gACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCCGGTGCCCGATGCGAGGTTGTTGCAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATAACgg                 >  1:353887/1‑250 (MQ=255)                                                                                                                                                                                                                              aCGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGGTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCGCCATTACCCCC‑AGCGCCCAGTTCCAGATCCCTTGCCTGAGTAAAAATACCGGaa               <  2:439628/251‑1 (MQ=255)                                                                                                                                                                                                                                   gcGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTGTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGAGTAAAAATACCGGAAATCCt          <  1:258859/250‑1 (MQ=255)                                                                                                                                                                                                                                      gcaccGACTACTCCGCGGCGGTGCTGGATGCCTCTTTACCCGCCGACTGTTGCGGGGTTTGGAAGGCCGTTGACGGGGGCTATACCTGCTACCCGCGTCAGGAGCCCGATGCGAGGGTGTTTGAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCGTAAGTAGGCGCTAAAATTCTTCACACCCACACCATTACCCCC‑AACGCCCACTTCCAGAACACTTGCCTGCTTCAAAATACCGGaacacctcac       >  2:480550/4‑243 (MQ=255)                                                                                                                                                                                                                                              tactCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCCGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGctcc  >  2:8622/1‑246 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACC  >  NC_000913/724‑1208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑