Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,349	C→T	E37E (GAG→GAA)	yaaA ←	DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,349	0	C	T	100.0%	218.4 / NA	69	E37E (GAG→GAA)	yaaA	DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/38);  total (31/38)

CGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAAT  >  NC_000913/6104‑6595                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cgcaGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATg                                                                                                                                                                                                                                                   >  1:95768/4‑251 (MQ=255)    aGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGAt                                                                                                                                                                                                                                                >  1:573374/1‑251 (MQ=255)         cccATACACGCCGGAAGGCATTCGCAAATGCTGTTGGGCAAAATCGAAACCCTCTTCGCTGAATGTTCCGCCCTGCAGGCCGGTTTAGACCTCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGGGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAAct                                                                                                                                                                                                                                           <  2:172034/250‑1 (MQ=255)                   ccGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAAtt                                                                                                                                                                                                                                 >  1:112450/1‑251 (MQ=255)                      gAAAGCATTCGCAAATGTTTTTGGGAAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGGGAAATCGGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTc                                                                                                                                                                                                                              <  2:210960/251‑1 (MQ=255)                      gAAAGCATTCGCAAATGCTGTTGGGCAAACTCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTc                                                                                                                                                                                                                              >  2:618735/1‑251 (MQ=255)                          gCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAAc                                                                                                                                                                                                                          >  2:419146/1‑251 (MQ=255)                            aTTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGCTCAACTGCTGGGAATTGTCTAAc                                                                                                                                                                                                                          >  1:470539/1‑249 (MQ=255)                             tccGCAAATGCGGTTGGGCAAAATCGATACCGTCTTGGCTGAAGGTGTCGGCCGGCAGGCCGGTGTAGACACCACCTTTAAACGCCAGAACCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCTTTGATTCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAg                                                                                                                                                                                                                        <  2:454560/248‑1 (MQ=255)                              tCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGGCTAACAGCt                                                                                                                                                                                                                      >  2:179280/1‑251 (MQ=255)                               cGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAACTCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGCTACCCGCCAGTTTGACGCTGATGCGAATCAGCGTGCTACTCTGCGGAGGCGTCAGTTTCCGCGCTTCATGAATCAACTGCTGGGAATTGTCTAACAGCTc                                                                                                                                                                                                                     >  1:585202/1‑251 (MQ=255)                                gCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTGGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTcc                                                                                                                                                                                                                    >  2:139891/1‑251 (MQ=255)                                         gtcggccAAAATCGATATCGTCCTCGCTGAAGCTTTCGGCCTGCAACCCGGTGTAGCCATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCACGGATCACCTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtc                                                                                                                                                                                                           >  2:432194/7‑250 (MQ=255)                                            gggCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTCAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGCTCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtat                                                                                                                                                                                                          >  2:7221/1‑249 (MQ=255)                                            gggCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtat                                                                                                                                                                                                          >  1:212427/1‑249 (MQ=255)                                            gggCAAAACCAAAATCGTCTTCGTTGAAGGTTTCGGCCTGAAAGCCGGTTTAGACATCCCCTTTAAACGCCAGAATCGCATGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAg                                                                                                                                                                                                        <  2:316412/251‑1 (MQ=255)                                              gCAAAATCGAAATCGTCTTCGCTGAAGTTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAgcg                                                                                                                                                                                                      <  1:352362/251‑1 (MQ=255)                                                     cgaaatcgTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCgtggtg                                                                                                                                                                                               >  1:363595/1‑251 (MQ=255)                                                      gaaatcgTCATCGCTGACGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGACATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGCAAG‑CGAGCGGCGTTGATACCCGCAAGTTTGTCGCTGATGCGCATAAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGaggtgg                                                                                                                                                                                              >  2:454724/1‑251 (MQ=255)                                                          tcgTCTTCGCGGAAGGTTTCGGCCTGCAAGCCGGGGTAGACATCCCCTTTAAACGCCAGAATCGCCGGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTAATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCa                                                                                                                                                                                           <  2:13716/250‑1 (MQ=255)                                                          ccgTCTTCGCGGAAGGTTTCGGCCGGCAAGCCGGTTTAGACACCACCTTTAAACGCCAGAATCGCCTGGGGGGCATTCTCGGGCGTAAAATCTGGCTGCCAGTCATGAAAG‑CGGGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCaa                                                                                                                                                                                          <  2:497460/250‑1 (MQ=255)                                                                tCGCTGAAGTTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACggg                                                                                                                                                                                      <  1:218781/249‑1 (MQ=255)                                                                 cGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCCGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCtt                                                                                                                                                                                   >  2:102138/1‑251 (MQ=255)                                                                      aaGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTa                                                                                                                                                                              <  1:115106/251‑1 (MQ=255)                                                                             cggccggcAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGGGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGc                                                                                                                                                                       <  2:173988/251‑1 (MQ=255)                                                                             cgcccggCAAGCCGGTGTAGACATCACCTTTAAACGCGAGAATCGCCGGGCGGGCTTTCCCCGGCGTAAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGGGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTCCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAg                                                                                                                                                                        <  2:469461/244‑1 (MQ=255)                                                                               gCCTGCAAGCCGGGGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGCGGCGTCAGTTTCAGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCgg                                                                                                                                                                     >  2:91775/1‑250 (MQ=255)                                                                                  ggaaggcgggTGTAGACACCACCTTAAGACCCAAGAAGCGCCGGGCGGGCATTCTCCGGCGGAAAATCTGCCGGCCAGCCATGAAAG‑CGAGCGGGGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTTGTAATCAAGCGtttt                                                                                                                                                                  <  2:328308/243‑1 (MQ=255)                                                                                   gcaggCCGGTGTAGACATCCCCTTTAACCGCAAGAATCGCCTGGCGGGCTTTCTCCGGCGTGAAATCTGGCTGCCGGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTc                                                                                                                                                                 <  2:386568/247‑1 (MQ=255)                                                                                   gCAAGCCGGTGCCGACATCAACTTTAAACGCCAGAATCGGCTGGCGGGCATTCTCCGGCGAGACATCTGGCTGCCCGTCATGAAAG‑CGAGCGGCGATGATACCCGACAGTTTGTCGCTGCTGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTCTAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGtttcc                                                                                                                                                                 >  2:45673/1‑249 (MQ=255)                                                                                      cgcgggTGTAGACACCACCTTTAAAACCAAAAATCGCCTGTCGGGTATTCCCCGGCGTAAAATCGGGCTGCCTCTCATGAAAG‑CGAGCGGCGTTGATTCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCa                                                                                                                                                              <  2:174509/247‑1 (MQ=255)                                                                                               agacaccacttgtAAACGCAAGAATCGCCGGGCGGGCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTGGGTAATCAAGCGTTTTCGCAGGTGAAATa                                                                                                                                                     <  2:311456/239‑1 (MQ=255)                                                                                                  cATCACCTTTAAACCCAAGAATCGCCGGGGGGGCATTCCCCGGCGTGAAATCGGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGa                                                                                                                                                  <  2:185474/251‑1 (MQ=255)                                                                                                              aCGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCAAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATcc                                                                                                                                      <  1:432761/251‑1 (MQ=255)                                                                                                                      atagCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGACAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTTATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGACTTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGGGGTCACCGGGCTTTGGTCATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTg                                                                                                                              >  2:399113/4‑251 (MQ=255)                                                                                                                         gCCTGGCGGGCATTCTCCGGCGTGAAATCCGGCTGCAAGTCATGACAG‑CGAGCGTCGATGATACCCGCCCGTTTGTCGCTGATGCGCATCCGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGACTTGTCTAACAGCTCCGGCAGCGTATAGCGCGAGGTGGTCAACGGGCTTTGGTAATCAAGCGCTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGc                                                                                                                             >  2:301475/1‑249 (MQ=255)                                                                                                                          ccTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAgg                                                                                                                          <  1:332856/251‑1 (MQ=255)                                                                                                                               ggggCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAAtt                                                                                                                     <  1:179280/250‑1 (MQ=255)                                                                                                                                ggccattcccgtgcgtaaactctgtctgCCAGACATGAAAG‑CTCGCGGCTTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGATGCGTCAGTTTCCGCGCTTCATGGATCAACGGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGGGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAAttt                                                                                                                    <  2:618806/231‑1 (MQ=255)                                                                                                                                  gCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTAt                                                                                                                  <  1:187362/251‑1 (MQ=255)                                                                                                                                      tctccGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCg                                                                                                              >  2:312708/4‑251 (MQ=255)                                                                                                                                      cccccGGGGTGAAATCGGGCGGCCAGTCATGAAAG‑CGGGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCg                                                                                                              <  2:230559/248‑1 (MQ=255)                                                                                                                                         ccgccttGAAATCTGGCTGCCTGTCAGGAAAG‑CGTGCGGCGTTGATACCCGCCAGGTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCTCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCTTATAGCGCGTGGTGGTCATCGGGCTTTTGTAAGCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATAGCCAGTCCTTTGAGGAAAGTTATGCCGACt                                                                                                           <  2:250782/245‑1 (MQ=255)                                                                                                                                         ccGGCGTGAAATCTGGCTGCCATTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCg                                                                                                              <  2:490714/248‑1 (MQ=255)                                                                                                                                         ccGGCGTAAAATCTGGCTGCCAGTCA‑GAAAGGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACt                                                                                                           <  2:112450/251‑1 (MQ=255)                                                                                                                                             cGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACttt                                                                                                         >  2:188699/1‑249 (MQ=255)                                                                                                                                              gTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGTTACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGTAATTTATGCCGACTTTAGc                                                                                                      <  2:462993/251‑1 (MQ=255)                                                                                                                                                       ggCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGCCTTTCGCaaaaaa                                                                                                >  2:345912/1‑248 (MQ=255)                                                                                                                                                         cTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCaaaaaacg                                                                                              >  2:440098/1‑246 (MQ=255)                                                                                                                                                              ggTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAaaagagaatgag                                                                                      <  2:545457/250‑1 (MQ=255)                                                                                                                                                                atcgtgaaggcgagggggtttGATGCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGt                                                                                     <  1:63480/232‑1 (MQ=255)                                                                                                                                                                        ggccagcgGCGTTGATCCCCGACAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGAt                                                                             <  2:472146/247‑1 (MQ=255)                                                                                                                                                                             gcggcgTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCATGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTtg                                                                        <  1:392443/251‑1 (MQ=255)                                                                                                                                                                               ggcgTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTtgtg                                                                      >  1:2197/1‑251 (MQ=255)                                                                                                                                                                                 cgTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGAt                                                                    <  1:102138/251‑1 (MQ=255)                                                                                                                                                                                      aTACCCGCCAGTTTGGCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTc                                                               <  1:239375/251‑1 (MQ=255)                                                                                                                                                                                                gTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAAca                                                     <  1:618735/251‑1 (MQ=255)                                                                                                                                                                                                 tttGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGAGTATAGCGCGTGGTGGTCAACGGGATTTGGTAAGCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGAAGGAAATTTATGCCGTGTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAAcat                                                    >  1:134444/1‑251 (MQ=255)                                                                                                                                                                                                    gTCGCTGATGCGCATCAGCGTGCTAATCTGCTGAGGCTTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCATCGTATAGCGCGTGGTGGGCAACGGGCTTTGGTACTCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTATCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAAcatca                                                  <  1:30322/250‑1 (MQ=255)                                                                                                                                                                                                             gcgcATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCAcgcg                                         <  1:312708/250‑1 (MQ=255)                                                                                                                                                                                                                     gCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGaa                                <  1:159700/251‑1 (MQ=255)                                                                                                                                                                                                                         gCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGCAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCt                            >  2:87428/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGAGCGATAGTTGTGATTACGCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCggc                      <  2:287054/249‑3 (MQ=255)                                                                                                                                                                                                                                   ggAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGat                  <  1:460336/251‑1 (MQ=255)                                                                                                                                                                                                                                     aGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCCGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGatat                >  2:576501/1‑251 (MQ=255)                                                                                                                                                                                                                                        cgtaaGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCg             >  2:550022/5‑251 (MQ=255)                                                                                                                                                                                                                                             gTTTCAGCGCTTCATGGATCCACTGATGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCAGGAGAAAGCTGGCGGCCGATATCCGGATa         >  2:320958/1‑250 (MQ=255)                                                                                                                                                                                                                                              tttCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGAGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAAc       >  1:377631/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gcgcTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCaac  >  1:447593/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAG‑CGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAAT  >  NC_000913/6104‑6595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑