| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 6,239 | G→T | A74E (GCG→GAG) | yaaA ← | DNA binding and peroxide stress response protein YaaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 6,239 | 0 | G | T | 87.7% | 173.6 / ‑1.9 | 74 | A74E (GCG→GAG) | yaaA | DNA binding and peroxide stress response protein YaaA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base T (33/31); minor base C (0/9); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTAT > NC_000913/5992‑6483 | gTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCg < 1:109470/251‑1 (MQ=255) tCAGCTTGTTGGTTATGCTACCTCCCCAGAAGTGATACAGATCTTTCCTCCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCAGTAAAGCGAGCGGGCGCAGTTCGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCACAATCGAAATCGTCTTCTCTGAAGGTTTCGGCCTTCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCgg < 1:125407/250‑1 (MQ=255) cAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCg < 1:487481/251‑1 (MQ=255) aGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCAGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGt > 1:205916/1‑251 (MQ=255) gCTTTTTGGGGATGATATCTCCCCAGAATTGATACAGATCTTGCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCATACGATAATGCTGCATTAAATCGAGCGGGGGCAGTACGCCATACAAGCCGGAAAGCATTGGCAAAGGCTGTG‑GTGCAAAATCGAAATCGTCTTCGCTGAAGGTGGCGGCCTGCAAGCCGGTTTAGACATCACCTTTAAACGCCAGAATCGCCTGGCTGGCAT‑TCTCCGGCGTg < 1:610482/251‑1 (MQ=255) tgttgGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGaa < 1:61594/250‑1 (MQ=255) tgttgGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGaa > 1:403872/1‑250 (MQ=255) ggTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAACGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTg > 1:237778/1‑251 (MQ=255) tctcCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTc > 2:247606/1‑251 (MQ=255) ctccAGAATTGGTACAGAACTTTTCCTCGTGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCAGTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTTGCGGGCGG‑TCTCCGGCGTTAAAGCTGGCTGACAGTCa < 1:501206/247‑1 (MQ=255) ccccAGAATTGATACAGATCTTTCCATCGTGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCAGTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAAGCGCCGGGCGTGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCAt < 2:64932/250‑1 (MQ=255) ccAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGaa < 2:433509/251‑1 (MQ=255) aaaTTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAagc < 2:173362/250‑1 (MQ=255) cAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCCGGCTGCCAGTCATGAAAGCGAGCGGCGt < 1:247757/251‑1 (MQ=255) aGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCGTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGtt < 1:290958/251‑1 (MQ=255) tttCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATAc > 1:287054/1‑251 (MQ=255) tgcccTCGTGCAGTCTCAATGCGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGTAATCGAGCGTCGTTGAtgc < 2:371235/248‑3 (MQ=255) cccgcGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATAccc < 1:400483/246‑1 (MQ=255) cccTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCg > 2:218781/1‑251 (MQ=255) gACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCCCCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATg < 1:179717/251‑1 (MQ=255) gACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGTCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTgctg > 1:40758/1‑248 (MQ=255) gACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGACATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATg > 1:386568/1‑251 (MQ=255) aCGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATgc > 1:497460/1‑251 (MQ=255) ccAGACGATAAGGCTACATTAAGTCGGGAGGGCGCAGTACGCCAAACAAGCCTGAAAGAATCCGCAAA‑GGTGTTTGGGAAAAATCGAAAGCGTCTGGTCTGAGGGTTTCTGCCGGCAAGCCGGTTTAGCCATCTCCTTGAACCCCTAGAATCGTCTGGCGGG‑ATGTCTCCGTCGTAAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCTTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTa < 2:33707/251‑1 (MQ=255) gACGATAAGGCTGCATAAAATCGAGCGGGCGAAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCGGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATc < 1:502037/251‑1 (MQ=255) aTAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCg < 1:287367/251‑1 (MQ=255) aagtgtccATTAAATCGAGCGGGCGCAGGACTCCATACAAGCCGGAAAGCTTTCGCAAATGCTGTT‑GGGAAAAACCGAAATCGTCTTCGCTGAAGGTTCCGGCCGGCAGGCGGGTGTAGACATCCCCTTTAAACGCCAGAATCGCCGGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCTTGAAAGCGAGCGTCGTTGAGACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGa < 2:570091/244‑1 (MQ=255) ggCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAgg > 1:192069/1‑251 (MQ=255) cTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTAGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGGGCGGCGTTGATACCCGCCATATTGGCGCTGATGCGCATAAGCGGGCTAATCTGCGGAGGCg > 2:353811/1‑251 (MQ=255) aTTAAACCGAGCGGGCGCAGTACGCCAAACAAGCCGAAAAGCATTCGCAAATGGGGTT‑GGGCAAAATCGAAATCGTCTTCGCGGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCATAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGTGCGGCGTTGATCCCCGCCATTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAg < 1:30890/251‑1 (MQ=255) cGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgc > 2:460336/1‑251 (MQ=255) gggcgggcgcAGTACCCCATCCAACCCGGAAAGCTTCCGCAAATGCTGTT‑GGGCAAAATCGAAACCGTCTTCGCTGAAGGTTTCGGCCGGCAGGCCGGTGTAGACATCACCTTTAACCTCAAGAATCGCCTGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGATCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCcgcg < 2:403872/249‑1 (MQ=255) cgggcgcAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCgcgctt > 1:261807/1‑249 (MQ=255) cgcaGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATg > 1:95768/4‑251 (MQ=255) aGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGAt > 1:573374/1‑251 (MQ=255) cccATACACGCCGGAAGGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAACCCTCTTCGCTGAATGTTCCGCCCTGCAGGCCGGTTTAGACCTCCCCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGGGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAAct < 2:172034/250‑1 (MQ=255) ccGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAAtt > 1:112450/1‑251 (MQ=255) gAAAGCATTCGCAAATGTTTTT‑GGGAAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCGCCGGCGGGAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTc < 2:210960/251‑1 (MQ=255) gAAAGCATTCGCAAATGCTGTT‑GGGCAAACTCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTc > 2:618735/1‑251 (MQ=255) gCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAAc > 2:419146/1‑251 (MQ=255) aTTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGCTCAACTGCTGGGAATTGTCTAAc > 1:470539/1‑249 (MQ=255) tccGCAAATGCGGTT‑GGGCAAAATCGATACCGTCTTGGCTGAAGGTGTCGGCCGGCAGGCCGGTGTAGACACCACCTTTAAACGCCAGAACCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCTTTGATTCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAg < 2:454560/248‑1 (MQ=255) tCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGGCTAACAGCt > 2:179280/1‑251 (MQ=255) cGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAACTCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGCTACCCGCCAGTTTGACGCTGATGCGAATCAGCGTGCTACTCTGCGGAGGCGTCAGTTTCCGCGCTTCATGAATCAACTGCTGGGAATTGTCTAACAGCTc > 1:585202/1‑251 (MQ=255) gCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTGGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTcc > 2:139891/1‑251 (MQ=255) gtcggccAAAATCGATATCGTCCTCGCTGAAGCTTTCGGCCTGCAACCCGGTGTAGCCATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCACGGATCACCTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtc > 2:432194/7‑250 (MQ=255) gggCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTCAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGCTCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtat > 2:7221/1‑249 (MQ=255) gggCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGtat > 1:212427/1‑249 (MQ=255) gggCAAAACCAAAATCGTCTTCGTTGAAGGTTTCGGCCTGAAAGCCGGTTTAGACATCCCCTTTAAACGCCAGAATCGCATGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAg < 2:316412/251‑1 (MQ=255) gCAAAATCGAAATCGTCTTCGCTGAAGTTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAgcg < 1:352362/251‑1 (MQ=255) caaaatcaaaatCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCCCCTTTAAACGCCAGAAGCGCCGGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCGGCCAGCCATGAAAGCGAGCGGCTTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCt < 2:500455/177‑1 (MQ=255) cAAAATCGCAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCt > 1:500455/1‑177 (MQ=255) cgaaatcgTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCgtggtg > 1:363595/1‑251 (MQ=255) gaaatcgTCATCGCTGACGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGACAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGCAAGCGAGCGGCGTTGATACCCGCAAGTTTGTCGCTGATGCGCATAAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGaggtgg > 2:454724/1‑251 (MQ=255) tcgTCTTCGCGGAAGGTTTCGGCCTGCAAGCCGGGGTAGACATCCCCTTTAAACGCCAGAATCGCCGGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTAATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCa < 2:13716/250‑1 (MQ=255) ccgTCTTCGCGGAAGGTTTCGGCCGGCAAGCCGGTTTAGACACCACCTTTAAACGCCAGAATCGCCTGGGGGGCAT‑TCTCGGGCGTAAAATCTGGCTGCCAGTCATGAAAGCGGGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCaa < 2:497460/250‑1 (MQ=255) tCGCTGAAGTTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACggg < 1:218781/249‑1 (MQ=255) cGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCCGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCtt > 2:102138/1‑251 (MQ=255) aaGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTa < 1:115106/251‑1 (MQ=255) cggccggcAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGGGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGc < 2:173988/251‑1 (MQ=255) cgcccggCAAGCCGGTGTAGACATCACCTTTAAACGCGAGAATCGCCGGGCGGGCTT‑TCCCCGGCGTAAAATCTGGCTGCCAGTCATGAAAGCGAGCGGGGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTCCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAg < 2:469461/244‑1 (MQ=255) gCCTGCAAGCCGGGGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGCGGCGTCAGTTTCAGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCgg > 2:91775/1‑250 (MQ=255) ggaaggcgggTGTAGACACCACCTTAAGACCCAAGAAGCGCCGGGCGGGCAT‑TCTCCGGCGGAAAATCTGCCGGCCAGCCATGAAAGCGAGCGGGGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTTGTAATCAAGCGtttt < 2:328308/243‑1 (MQ=255) gcaggCCGGTGTAGACATCCCCTTTAACCGCAAGAATCGCCTGGCGGGCTT‑TCTCCGGCGTGAAATCTGGCTGCCGGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTc < 2:386568/247‑1 (MQ=255) gCAAGCCGGTGCCGACATCAACTTTAAACGCCAGAATCGGCTGGCGGGCAT‑TCTCCGGCGAGACATCTGGCTGCCCGTCATGAAAGCGAGCGGCGATGATACCCGACAGTTTGTCGCTGCTGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTCTAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGtttcc > 2:45673/1‑249 (MQ=255) cgcgggTGTAGACACCACCTTTAAAACCAAAAATCGCCTGTCGGGTAT‑TCCCCGGCGTAAAATCGGGCTGCCTCTCATGAAAGCGAGCGGCGTTGATTCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCa < 2:174509/247‑1 (MQ=255) agacaccacttgtAAACGCAAGAATCGCCGGGCGGGCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTGGGTAATCAAGCGTTTTCGCAGGTGAAATa < 2:311456/239‑1 (MQ=255) cATCACCTTTAAACCCAAGAATCGCCGGGGGGGCAT‑TCCCCGGCGTGAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGa < 2:185474/251‑1 (MQ=255) aCGCCAGAATCGCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCAAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATcc < 1:432761/251‑1 (MQ=255) atagCCTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGACAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTTATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGACTTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGGGGTCACCGGGCTTTGGTCATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTg > 2:399113/4‑251 (MQ=255) gCCTGGCGGGCAT‑TCTCCGGCGTGAAATCCGGCTGCAAGTCATGACAGCGAGCGTCGATGATACCCGCCCGTTTGTCGCTGATGCGCATCCGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGACTTGTCTAACAGCTCCGGCAGCGTATAGCGCGAGGTGGTCAACGGGCTTTGGTAATCAAGCGCTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGc > 2:301475/1‑249 (MQ=255) ccTGGCGGGCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAgg < 1:332856/251‑1 (MQ=255) ggggCAT‑TCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAAtt < 1:179280/250‑1 (MQ=255) gCAT‑TCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTAt < 1:187362/251‑1 (MQ=255) | GTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTT‑GGGCAAAATCGAAATCGTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCAT‑TCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTAT > NC_000913/5992‑6483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |