Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,806	G→A	F218F (TTC→TTT)	yaaA ←	DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,806	0	G	A	100.0%	217.0 / NA	68	F218F (TTC→TTT)	yaaA	DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/30);  total (38/30)

CAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCAT  >  NC_000913/5561‑6050                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACg                                                                                                                                                                                                                                                  >  1:444429/1‑251 (MQ=255)      ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCATTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACtca                                                                                                                                                                                                                                             <  1:499542/251‑1 (MQ=255)         gaggCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCTTTAACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGGTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACtcatca                                                                                                                                                                                                                                          <  2:581382/248‑1 (MQ=255)             cGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGAcc                                                                                                                                                                                                                                      >  2:563882/1‑251 (MQ=255)              gACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACcg                                                                                                                                                                                                                                     >  1:121295/1‑251 (MQ=255)                cGCTGGCGCGTCTTATCCGGCCTACGTGAATTACGCCATATATTGCATCTGCATGCTTTTGTAGGCCGGATACGGCGTTCACGCCGCATCCGGTATTGACTGCAAACTTAACGCTGCTCGTCGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGACGTCACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGCCTCATCAGACagcg                                                                                                                                                                                                                                   >  2:30890/1‑251 (MQ=255)                cGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACcgcg                                                                                                                                                                                                                                   <  1:439937/251‑1 (MQ=255)                 gCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAACACGACTCATCAGACcgcat                                                                                                                                                                                                                                  >  1:371235/1‑249 (MQ=255)                            ttATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGc                                                                                                                                                                                                                       >  1:23864/1‑251 (MQ=255)                              aTCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATACTAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGt                                                                                                                                                                                                                     >  2:400483/1‑251 (MQ=255)                                 aGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCCGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGt                                                                                                                                                                                                                     >  1:343904/1‑248 (MQ=255)                                    ccTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGc                                                                                                                                                                                                               >  2:325508/1‑251 (MQ=255)                                          tgaaTTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtgatt                                                                                                                                                                                                          >  2:488606/3‑249 (MQ=255)                                                     atatatTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTg                                                                                                                                                                                              <  2:170605/251‑1 (MQ=255)                                                         atTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCg                                                                                                                                                                                           <  1:344895/250‑1 (MQ=255)                                                                cTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTtctc                                                                                                                                                                                   >  1:545457/1‑251 (MQ=255)                                                                  gCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATACAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCAt                                                                                                                                                                                 >  2:533227/1‑251 (MQ=255)                                                                  gCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCAt                                                                                                                                                                                 <  1:343330/251‑1 (MQ=255)                                                                  gCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCAt                                                                                                                                                                                 >  2:502037/1‑251 (MQ=255)                                                                  gCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTAGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTtctc                                                                                                                                                                                   <  1:458069/249‑1 (MQ=255)                                                                   cATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATc                                                                                                                                                                                <  1:344232/251‑1 (MQ=255)                                                                   cATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGAAAACTTAACGCTGATCGTAGCGTTTAAACACAAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGCTTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATc                                                                                                                                                                                >  1:577858/1‑251 (MQ=255)                                                                           tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAac                                                                                                                                                                         <  2:543858/250‑1 (MQ=255)                                                                             tAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAGGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCGGCCGTTTTTCAATAATAAAACGCCTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTATTCGAGGaccacc                                                                                                                                                                      >  2:136384/1‑251 (MQ=255)                                                                               ggccggATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCgg                                                                                                                                                                    >  1:33707/5‑251 (MQ=255)                                                                               ggccggATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTACCGTTCTTCTCATCGAGGAACACCgg                                                                                                                                                                    >  2:108103/5‑251 (MQ=255)                                                                                       tatgcctccaagccgcATCCGGCATTGCCTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGATGCTGCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGTTTTGGTCCGCCGATTTTCAATAATAAAACGCCTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCGTAAAATTGCCGTCCTtctcctc                                                                                                                                                                                <  2:426969/220‑4 (MQ=255)                                                                                       aaGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGAGTTTAAACACCAGGTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAAACAGTCAGTTGCTCTGGGTTGGTCAGCCGATTTTCAATATTAAAACGACTCATCAGACCGCGAGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATc                                                                                                                                                                                >  1:426969/1‑231 (MQ=255)                                                                                        aGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑At                                                                                                                                                          <  1:198795/251‑1 (MQ=255)                                                                                            gTTCACGCCGCCTCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCg                                                                                                                                                      >  2:239505/1‑251 (MQ=255)                                                                                               cACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGc                                                                                                                                                    >  1:236789/1‑250 (MQ=255)                                                                                                              aatcacagCAAACTTATCGCTGCGCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGATTCATCAAAGAAGTAAACTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGTCGATTTTCAATAATAATACCACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTTCCGTTCTTCTCATCGAGGAACACCGCCTTGATA‑ATCTCGTCATTCAATTTCTTCGGc                                                                                                                                    <  2:478228/244‑1 (MQ=255)                                                                                                              aTTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGc                                                                                                                                    >  1:297170/1‑251 (MQ=255)                                                                                                                 gACTGCAAACTTAACGCTGCTCGGAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTc                                                                                                                                 <  1:275291/251‑1 (MQ=255)                                                                                                                   cTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCAc                                                                                                                               >  2:354485/1‑251 (MQ=255)                                                                                                                   cTGCAAACTTAACGCTGCTCGTAGAGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGAATCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTG‑TATATCTCGGCATTCAATTTCTTCGGCttaac                                                                                                                               >  2:354477/1‑248 (MQ=255)                                                                                                                     gCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCg                                                                                                                             >  1:462993/1‑251 (MQ=255)                                                                                                                       aaaCTTAACGCTGCTCGTAGCGTTTAAACACCAGTGCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCGCGGCATTCAATTTCATCGGCTTCACCGAt                                                                                                                           <  1:176286/251‑1 (MQ=255)                                                                                                                                   gctCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGGTTTAAAATACTc                                                                                                                >  2:247757/1‑250 (MQ=255)                                                                                                                                         aGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGAc                                                                                                         >  1:570091/1‑251 (MQ=255)                                                                                                                                           cGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTTATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑AGCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCAGCTGACGc                                                                                                       <  2:43710/251‑1 (MQ=255)                                                                                                                                                     ccAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATc                                                                                             >  1:433509/1‑251 (MQ=255)                                                                                                                                                          tCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATcaccac                                                                                        >  1:173362/1‑251 (MQ=255)                                                                                                                                                                  gCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCg                                                                                 <  1:325508/250‑1 (MQ=255)                                                                                                                                                                    tGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCt                                                                              >  1:618806/1‑251 (MQ=255)                                                                                                                                                                                 atcaAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAgct                                                                 <  2:444429/251‑2 (MQ=255)                                                                                                                                                                                      agaagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCg                                                            >  1:409236/1‑251 (MQ=255)                                                                                                                                                                                        aagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGGGCTGCGAGCGCCTCGtt                                                          <  1:462938/251‑1 (MQ=255)                                                                                                                                                                                         agTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGCTTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCGCCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTc                                                         >  1:33868/1‑251 (MQ=255)                                                                                                                                                                                            aaCCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCCGACTCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAg                                                       <  1:101231/250‑1 (MQ=255)                                                                                                                                                                                                  cGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCttgttg                                                >  1:437641/1‑251 (MQ=255)                                                                                                                                                                                                       ttAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCCGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGttcttc                                                                                                                                                                                     >  2:231442/1‑114 (MQ=255)                                                                                                                                                                                                       ttAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGttcttc                                                                                                                                                                                     <  1:231442/114‑1 (MQ=255)                                                                                                                                                                                                        tAAATCCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGtgat                                           <  1:302680/250‑1 (MQ=255)                                                                                                                                                                                                              cagtcagtTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATAtct                                    <  1:128946/251‑1 (MQ=255)                                                                                                                                                                                                                gtcagtTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCTCATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTcc                                  <  2:149492/251‑1 (MQ=255)                                                                                                                                                                                                                                tGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTGCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAg                   <  2:513330/250‑1 (MQ=255)                                                                                                                                                                                                                                 ggTCAGCCGATTTTCACTAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATATTACATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATAc                     >  2:290958/1‑247 (MQ=255)                                                                                                                                                                                                                                 ggTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGAt                 <  1:136384/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATGTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTTATACATATc                <  2:143343/251‑1 (MQ=255)                                                                                                                                                                                                                                    cAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTACCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCt               <  1:108103/250‑1 (MQ=255)                                                                                                                                                                                                                                      gCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTGTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTc            <  1:445762/251‑1 (MQ=255)                                                                                                                                                                                                                                        cGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTccc          >  2:352362/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTc        >  2:110544/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttttCAATAATAAAACGACTCCTCAGACCGCGTGCTTTCTTAGCGTCGAAACTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGTCGCCAGATTAATCACCCCATTCTCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCg       >  2:432761/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttttCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCACTCg       >  1:472146/1‑251 (MQ=255)                                                                                                                                                                                                                                            tttCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCg       <  2:121295/250‑1 (MQ=255)                                                                                                                                                                                                                                                aataatAAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCAt  <  1:563882/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ataataAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAACTACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTTCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGCTATCTCCCCAGACTTGATa                      >  2:165959/1‑230 (MQ=255)                                                                                                                                                                                                                                                 ataataAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATTATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAAGACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATa                      <  1:165959/230‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATA‑ATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACGCCAGATTAATCACCACATTATCGCCTTGTGCTGCGAGCGCCTCGTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCAT  >  NC_000913/5561‑6050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑