Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,652	C→T	C350C (TGC→TGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,652	0	C	T	97.8%	283.9 / NA	90	C350C (TGC→TGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/46);  minor base A (2/0);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACA  >  NC_000913/4620405‑4620898                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGAt                                                                                                                                                                                                                                                     <  1:557375/251‑1 (MQ=255) gTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCCGGTGATAACAACATCGTCTTCACCAACTGCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCTACGTCGCCGGTTATGCTGCGGGTCTGGTGCTGTTCGACCGCCGTCTGCCGGAGCAGATGTCTCTGCTGCGGGATGACGACATCCTGAACCTCCCTGATTACCACGGTTGTGAt                                                                                                                                                                                                                                                     <  2:580229/251‑1 (MQ=255)   aaaaCCAAGAAAGTGAAAGCAACGGGACTGGACGCGCTGTTTG‑ACGCCACCACCAAAGAGATGAAAGAAGCGGGTGATAACGCCATCGTCTTCACCAACTTCGTTGACTTCGACTCTGCCTGGGGCCACCGTCGAGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGAGCCTCGTTGCTGACCACGTTTGTGATc                                                                                                                                                                                                                                                    <  2:61714/247‑1 (MQ=255)   aTCACCAAGACAGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGCCCCCGGTTGTGATcc                                                                                                                                                                                                                                                   >  2:594595/1‑251 (MQ=255)   aTCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATcc                                                                                                                                                                                                                                                   >  2:144981/1‑251 (MQ=255)            aaaGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGCTCACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGAc                                                                                                                                                                                                                                          >  1:611392/1‑251 (MQ=255)             aaGTGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGGAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGAcc                                                                                                                                                                                                                                         <  1:177778/251‑1 (MQ=255)              aGTGAAAGCAACTGACCTGGACGCGCT‑TTTGTACGCCCCCAACAAAGAGATGAAAGAAGCGGGTGATAACCCCCTCGTCTTCACCAACTGCGTTGACTTCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGTTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACGGCTGACCACGGTTGTGATCCGACTTGGACCg                                                                                                                                                                                                                                        <  2:400090/251‑1 (MQ=255)                tGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGCTGAAAGAAGCGGGTGATAACACCATCGTCTTCACCACCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGCCATCCTGATACTCACTGCTGACCACGGTTGTGATCCGACCTGGACCggg                                                                                                                                                                                                                                      >  1:605860/1‑250 (MQ=255)                tGAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGGTGACGCCATCCTGATCCTCACTGCTGTCCACGGTTGTTATCCGACCTGGACCGGt                                                                                                                                                                                                                                      >  2:312386/1‑251 (MQ=255)                 gAAAGCAACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGCGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGAGATCCGCCCTGGACCGGt                                                                                                                                                                                                                                      >  2:549050/1‑250 (MQ=255)                           ggCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCacac                                                                                                                                                                                                                           >  1:389947/1‑251 (MQ=255)                                     cgcTGGTTG‑ACGCCACCATCAAAGCGTTGAAAGAAAATAGAGCTAACACCATCGTCTTCACCAACTTAGTTGACTGCGAGTCTTCCTGGGGCCACCGTCCCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCCCGATGACGACATCCTGCTCCTCACTGCTGACCCCGGTTGTGATCCGCACTGGACCGGTCCTGACCACCCGCGTGcaca                                                                                                                                                                                                                  >  2:395875/1‑250 (MQ=255)                                      gcTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAacac                                                                                                                                                                                                                 >  1:422354/1‑250 (MQ=255)                                       cTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGCTCGACCGCCGTCGGCCGGAGCTGATGTCTCTGCTGCGCGATGAAGACATCCTGATCCACACTGCTGACCACGGATGTGATCCGACCTGGACCGGTACTGAACACACGCGTGAACACAt                                                                                                                                                                                                               >  1:107749/1‑251 (MQ=255)                                        ggTTTG‑ACGCCACCAGCAAAGAGATTAAAGAAGCGGGTGATAGCACCATCGTCTTCACCAACTTCTTTGACGTCGACTCTTCCTGGGCCCCCCGTCGCGACGTCGCCGGTTATGCTGCTGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCGGCTGCGCGATTACGACATCCTGATCCTCACTTCTTACCACGGTTGTGATCCGACCTGGACCGGTGCTGACCACACGCGTGAACACAtt                                                                                                                                                                                                              <  2:53400/250‑1 (MQ=255)                                          tttG‑AAGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGCCTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTCTGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGCCATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGCCCGGCACTGACCACACGCGTGAACACATTcc                                                                                                                                                                                                            >  2:623795/1‑251 (MQ=255)                                             g‑ACGCCACCAGCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCgg                                                                                                                                                                                                          <  1:129480/250‑1 (MQ=255)                                                  cccccagcAAAGAGAGGAAAGTAGCTGGTGATAACACCATCGTCTTCACCAACTTCGTTGGCTGCGACGCTTCCTGGTGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCTCGGTTGTGATCCGACCTGGACTGGTACTGACCCCACGCGTGAACACATTCCGGTACTg                                                                                                                                                                                                     <  2:175857/244‑1 (MQ=255)                                                    accaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                                                                                   <  1:73192/251‑1 (MQ=255)                                                        acAAAGATATGAGAGAAGCGGGTGATAACTCCATCGTCTTCACCAGCTGCGTTGACTTCACCGCGTCCTGGGGTCACCGTCGCGACGTCGCCGTTTATGCTGCGGGCCGGGAGCTGTTCGACCGCCGTCGGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCGGCTCCTCACTGCTGACCACGGTTGTGATCCGACCTGTACTGGTTCTGACGACACGCCTGAGCACGTTCCggggctggtttac                                                                                                                                                                                               <  2:300382/250‑12 (MQ=255)                                                          ccagaggtgacagcagCGGGTGCTACAACCATAGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCCTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACTTCCTGATCCTCACTGCTGCCCACAGTTGTGATCCGACATGGACCGGTGCTGACCTCACGCGTGAACCCATTCCGGGACTGGtttacgc                                                                                                                                                                                             >  2:69526/15‑245 (MQ=255)                                                            agagATGAAAGAAGCGCGTGATAACACCATCGTCTTCACCAACTTCGTTGACTGCGACTCTTCCTGGGACCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGACGTCTGCCGGAGCTGATGTCTCTTCTGCGCGATGACGACATCCTGATCCGCACTGCTGCCCCCGGTTGTGATCATACCTGGACCGGTACTGACCACACGCGTGAACACAATCCGATACTGGtttacgtac                                                                                                                                                                                           >  2:499029/1‑243 (MQ=255)                                                                 tGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGaa                                                                                                                                                                                       >  1:512661/1‑250 (MQ=255)                                                                 ggAAAAAAGCGGCTGATACCACCATCGTCTCCACCAACGTCGTTGACGTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCTGTTATGCTTCGGGTCTGGAGCTGTGCGTCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCCCGCTGACGACATCCGGATCCTCACTGCTGACCACGGTTTTGAGCCGACCTGGACCGGTACTGTCCACGCGCGTGACCACATTGCGGTACTGGTTTACGGCCTGaaa                                                                                                                                                                                      <  2:56211/250‑1 (MQ=255)                                                                   aaaGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCATGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGCCCGGTACTGACCACACGCGTGAACACCTTCCGGAACTGGTTTACGGCCCGAAAGt                                                                                                                                                                                    >  1:389566/1‑251 (MQ=255)                                                                           gggTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCgg                                                                                                                                                                            <  1:559371/251‑1 (MQ=255)                                                                             gTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGc                                                                                                                                                                          <  2:345695/251‑1 (MQ=255)                                                                               gATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTc                                                                                                                                                                        <  1:156994/251‑1 (MQ=255)                                                                               gATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTc                                                                                                                                                                        >  2:309750/1‑251 (MQ=255)                                                                                aTAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTc                                                                                                                                                                        >  1:393586/1‑250 (MQ=255)                                                                                   acacCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTg                                                                                                                                                                    <  2:96920/251‑1 (MQ=255)                                                                                     acCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTggg                                                                                                                                                                  >  1:171225/1‑251 (MQ=255)                                                                                     acCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTggg                                                                                                                                                                  <  1:287228/251‑1 (MQ=255)                                                                                        aTCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGCGCCACCGTCGCGACGTCGACGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGCTGTCTCTGCTGCGCGCTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGCCCTGGACCGGTCCTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCACGAAAGTAAAACCGGGCTCACTGGGTc                                                                                                                                                                >  2:547830/1‑250 (MQ=255)                                                                                          cggcTTCACCAACTTCGTTGACTTCGACTCTCCCTGGGGCAACCGTCGCGACGTCTCCGGGTATGCTGCGGGTCTGGAGCTGTTCGCCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAACCCGGGCTCACTGGGTCacc                                                                                                                                                             <  2:140476/248‑3 (MQ=255)                                                                                            tCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGt                                                                                                                                                           >  1:197037/1‑251 (MQ=255)                                                                                                 aCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGGCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGAc                                                                                                                                                                                                                                                <  1:328009/161‑1 (MQ=255)                                                                                                 aCCAACTTCGTTGACTTCGACTCTTCCTGGGGCAACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGAc                                                                                                                                                                                                                                                >  2:328009/1‑161 (MQ=255)                                                                                                   cAACTTCGTTGACTTCGCATCTCCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTCCCGGAGCTGATGTCTCTGCGGCGCGATGACGACCTCCTGATCCTCACTGCTGACCACGGTTGGGATCCGACCTGGCCCGGTACTGACCACACGCGTGACCACATTCCGGGACTGGTTTACGGCCCGACAATCACACCGGGCTAACTGGGCCACCGTGAAACCt                                                                                                                                                    >  2:275274/1‑251 (MQ=255)                                                                                                        tCGTTGACTTCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTcgc                                                                                                                                                <  2:457224/250‑1 (MQ=255)                                                                                                          gTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGgg                                                                                                                                             >  2:512661‑M2/1‑249 (MQ=255)                                                                                                          gTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGATGCGGGTCTGGAGCTGTACGACCGCCGTCTGACGGAGCTGTTGTCTCTGCTGTGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGATAGTAAAACCGGGCTCACTGGGTCACCGTGAACCCTTcgctgg                                                                                                                                             >  1:503917/1‑248 (MQ=255)                                                                                                             gACTTCGACTCTTCCTGGGGCCCCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCa                                                                                                                                                               <  2:179997/230‑1 (MQ=255)                                                                                                             gACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCa                                                                                                                                                               >  1:179997/1‑230 (MQ=255)                                                                                                               cTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatg                                                                                                                                        <  1:274302‑M2/251‑8 (MQ=255)                                                                                                                ttCGCCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGCAACCTTCGCGgggggtgc                                                                                                                                       <  2:338484‑M2/251‑9 (MQ=255)                                                                                                                   gACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCTCGggtgatgctgc                                                                                                                                    >  2:360831‑M2/1‑240 (MQ=255)                                                                                                                         tCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaactt                                                                                                                              <  1:140255‑M2/251‑18 (MQ=255)                                                                                                                         gccTGGGTCCGCCGTCGCGACGTCGCCGGTTATGCTTCTGTTCTGGAGCGGTGCGACCGCCGTCTGCCGGAGCTGATGCCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTTACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCTGTACTGGTGTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACAGTGAAACCTTCGCgggttatgctgacaactt                                                                                                                              <  2:503917/250‑17 (MQ=255)                                                                                                                             ggggCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCAGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactg                                                                                                                          >  1:594595‑M2/1‑230 (MQ=255)                                                                                                                              gggCCCCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCCCTGCTGACCACGGTTGTGATTCGCCCTGGACCGGTACTGCCCACACGCGTGAACACCGTCCGGTACTGGTTTACGGTGCGACAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactga                                                                                                                         >  1:383542‑M2/1‑229 (MQ=255)                                                                                                                               ggCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgctgctgccaacttactgat                                                                                                                        <  2:392225‑M2/251‑24 (MQ=255)                                                                                                                                gCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGACACCTTCGCGggggatgctgccaccttactgatt                                                                                                                       <  1:276073‑M2/251‑25 (MQ=255)                                                                                                                                        cgcgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggggatgctgccaacttactgatttagtgtat                                                                                                               <  2:280509‑M2/251‑33 (MQ=255)                                                                                                                                             cGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatgg                                                                                                          <  1:477435‑M2/251‑38 (MQ=255)                                                                                                                                             cGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGAAGACGACATCATGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGGCACCGTGAAACCTTCGCGggtgatgctgccaacttactgaattagtgtatgatgg                                                                                                          <  1:282347‑M2/251‑38 (MQ=255)                                                                                                                                                 gCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtt                                                                                                      >  2:49901‑M2/1‑210 (MQ=255)                                                                                                                                                       tATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttg                                                                                                  <  1:546654‑M2/249‑46 (MQ=255)                                                                                                                                                          gctgcgggtCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggt                                                                                              >  2:197037‑M2/4‑201 (MQ=255)                                                                                                                                                               gggTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctcca                                                                                        >  2:390006‑M2/1‑196 (MQ=255)                                                                                                                                                                ggTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccag                                                                                       >  1:235811‑M2/1‑195 (MQ=255)                                                                                                                                                                      gagctgtTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgcgccagtggctt                                                                                 >  1:493425‑M2/4‑189 (MQ=255)                                                                                                                                                                       agctgTTCGACCGCCGTCTGCCCGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGATCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttc                                                                                <  1:429554‑M2/249‑64 (MQ=255)                                                                                                                                                                        gctGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttct                                                                               >  1:76531‑M2/2‑187 (MQ=255)                                                                                                                                                                               gACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGCAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggggatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttct                                                                         <  1:538169‑M2/250‑71 (MQ=255)                                                                                                                                                                                         tGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtcc                                                              <  1:297408‑M2/251‑82 (MQ=255)                                                                                                                                                                                          gCCGGAGCTGATTTCTCTGCTGCGCGATGACGACATCCTGATCTTCACTGCTGACCTCGGTTGAGATCCGACCTGGACCGGTACTGACCACAAGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgttttggaggtgctccagtggcttctgtttctatcagctgt                                                                >  2:129475‑M2/1‑169 (MQ=255)                                                                                                                                                                                            cGGAGCTGATGTCTCGGCTGCGCGATGACGGCATCCTGAGCCTCACTGGTGACCACGGTTGTGAGCCGACCTTGACCGGTACTGACCACACGCGTGAACCCATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtgtttgaggtgctccagttgcttctttttctatcagctgtccctc                                                           >  2:389566‑M2/1‑167 (MQ=255)                                                                                                                                                                                                   gATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcc                                                          <  1:374873‑M2/245‑86 (MQ=255)                                                                                                                                                                                                   gATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcc                                                          >  2:374873‑M2/1‑160 (MQ=255)                                                                                                                                                                                                     ggtCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgagggtgttttggaggtgcgccagtggcttctgtttctatcagctctcccgcctgttcagc                                                  >  2:409528‑M2/2‑158 (MQ=255)                                                                                                                                                                                                          ctgctgCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgcgccagtggcttctgtttctatcagctgtccctcctgttcagctac                                               >  2:546654‑M2/1‑153 (MQ=255)                                                                                                                                                                                                            gctgcGCGATGACGACATCCTGCTCCTCACTGCTGACCGCGGTTGTTATCCGACCTGGTCCGGTACTGTCCACACGCGTGAACTCATTCCGGTACTGTTTTACGGCCCGAAAGTCAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttacttatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctccggttcagctact                                              >  2:184498‑M2/1‑151 (MQ=255)                                                                                                                                                                                                              tgcGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacgg                                         <  1:260126‑M2/251‑103 (MQ=255)                                                                                                                                                                                                                   gATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTAATGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtgg                                    <  2:419059‑M2/251‑108 (MQ=255)                                                                                                                                                                                                                          acATCATGATCCTCCCTGCTGAACACGGTTGTGCTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgctttagtgtatggtggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtg                              <  1:168843‑M2/250‑114 (MQ=255)                                                                                                                                                                                                                           cATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaac                            <  1:373666‑M2/251‑116 (MQ=255)                                                                                                                                                                                                                           cATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACCGGTTTACGGCCCGACAGTACAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgacaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtacctcctgttcagctactgacggggtggtgcgtaac                            <  1:398772‑M2/251‑116 (MQ=255)                                                                                                                                                                                                                                tGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggca                        <  1:152807‑M2/250‑120 (MQ=255)                                                                                                                                                                                                                                   tCCTCAGTGCTGACAACGGTTGTGATCCGCCCTGGACCGGTACTGAAAACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGAAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaaattccagatttagtgtatgatggtgtttttgaggtgctccagtggcttttgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggaaca                      <  2:555308‑M2/249‑122 (MQ=255)                                                                                                                                                                                                                                   tCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggcggtgcgtaacggcaaa                      <  1:375793‑M2/249‑122 (MQ=255)                                                                                                                                                                                                                                      tCACTGCTGAACACGGTTGTGATCCGCCCTGGACCGGTACTGCCCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcac                 <  2:592880‑M2/251‑127 (MQ=255)                                                                                                                                                                                                                                        actgctgACCACGGTTGTGATCTGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTCCTGGGTTAAGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGAGACACCTTCGCGggtgatgctgcaaacttactgctttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccg               <  1:405525‑M2/248‑129 (MQ=255)                                                                                                                                                                                                                                        actgctgACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccg               >  1:549050‑M2/4‑123 (MQ=255)                                                                                                                                                                                                                                           gCTGACCACTGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgcc             >  1:267927‑M2/1‑120 (MQ=255)                                                                                                                                                                                                                                            cTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccgg           <  1:171702‑M2/251‑133 (MQ=255)                                                                                                                                                                                                                                               aCCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggac         <  1:334784‑M2/250‑135 (MQ=255)                                                                                                                                                                                                                                                    ggTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagc   >  1:233983‑M2/1‑111 (MQ=255)                                                                                                                                                                                                                                                     gTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcg  >  1:587815‑M2/1‑110 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       GTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTG‑ACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTACCCCACACA  >  NC_000913/4620405‑4620898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑