Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,256	C→T	G218G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,256	0	C	T	98.6%	207.6 / NA	73	G218G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (33/39);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAG‑AAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGG‑TAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCA  >  NC_000913/4620024‑4620500                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      aCCTCGGTAACTGCCACTCTTCCGGTACGGACATTCTGGATCAGCTGGGCGAAGAGCACATGCAAACCGGCAAGCCGATTTTCTATACCTCCGCAGACCCCGTGTTCCAGCTTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACCTCGGTCGCGTTATCGCTCGACCCGTTATCGGCGCCAAATCCGGTTACTTc                                                                                                                                                                                                                                     >  2:278604/1‑251 (MQ=255)                   ctTCCGGTACGGTCATTTGGGCTCAGCTGGGCGAAGAGCACATGAAATCCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTTGTCCAGAGTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGTCCTACGGCGGCTACAACATCGGTCTTGTTATCGCTCGTCCGTTTATCGGTGACAAGGCCGGTAACTTCCATCGTACCGGTatcc                                                                                                                                                                                                                     <  2:360515/249‑4 (MQ=255)                     tCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGt                                                                                                                                                                                                                   <  1:137081/249‑1 (MQ=255)                      acGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAAAATCGGTCGTGTTATAGCTCGTCCGTTTTTCGGTGACCAATCCGGGAACTTCCAGCGTACCGGTAAACGTCa                                                                                                                                                                                                                 >  2:247827/2‑250 (MQ=255)                                       gATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCCGCGTCCCGGTAACCGTCACGACCTGGCTGTTGAgcc                                                                                                                                                                                               >  1:270021/1‑251 (MQ=255)                                                ggCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTTAGACGCCCGCacc                                                                                                                                                                                      >  2:559371/1‑251 (MQ=255)                                                  cGAAGAGCACAGGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACTGCGGCTACAACATCGGTCGTGTTATCGCGCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCaccga                                                                                                                                                                                    <  1:369655/251‑1 (MQ=255)                                                     agaacacaTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCaccgaccg                                                                                                                                                                                 <  2:83932/247‑1 (MQ=255)                                                         cacaTGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGtgct                                                                                                                                                                             <  1:295173/251‑1 (MQ=255)                                                                 aaCCGGCAAGCCGATTTTCTATACCTCCGCTGACTACGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGGTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTACCTGCCAGCGTACCGGGAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAAc                                                                                                                                                                     <  2:321881/251‑1 (MQ=255)                                                                   ccGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAATCCGGTAAATTCAAGCGTACCGGTAACCGGCAAGACCTGGATGTTGAGCCGCCAGCACCGACCGTGCGGCAGAAACTg                                                                                                                                                                   >  1:580229/1‑251 (MQ=255)                                                                     ggCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGt                                                                                                                                                                 <  1:599437/251‑1 (MQ=255)                                                                       cAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGACCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACAAACGGCGGCTAAAACATCGGTCGTGTTATAGCTCGTCCGTTTATCGGTGACAAAACCGGTAACTTCCAGCGTACCGGTAACCGTCACAACCTGGCTGTTGAGCCGCCCGCACCGACCGTGCAGCAGAAACTGGTtg                                                                                                                                                               >  1:497988/1‑251 (MQ=255)                                                                        aaGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGACGCCCGCACCGACCGTGCTGCAGAAAATGGTtg                                                                                                                                                               >  1:243332/1‑250 (MQ=255)                                                                            cGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGTCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGaa                                                                                                                                                          <  1:13379/251‑1 (MQ=255)                                                                                     ataCCTCCGCTGACTCCGTGTTACAGCTTGCCTGACACGAAGGAAACTTT‑GGCCTGGATAAACTCTACGACCTCTGCGAAATCGCCCGTGAAGAGCTGACCAACGCCGGCTACAACATCGGTCGTGATATCGGTCGTCCGTTTATCCGTGACAAAGCCGGTAACTTCCAAAGCACCGGTAACACTCACGCCCTGGCTGGTGTGCCGCCAGCACCGACCGTGCTGCAGACACTGGTTGCTGAAAAacagggg                                                                                                                                                 >  2:267927/1‑247 (MQ=255)                                                                                       aCCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGGAACCGTCCCGAACTGGCTGTTTGGCCGCCAGCCCCGCCCGTGCTGCAGCAAATGGGTGATGAAAAACACggtc                                                                                                                                                >  2:177778/1‑248 (MQ=255)                                                                                        ccaccGCTGACCCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAg                                                                                                                                              <  2:480110/248‑1 (MQ=255)                                                                                         cTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGa                                                                                                                                                                                                  >  1:254278/1‑198 (MQ=255)                                                                                         cTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGa                                                                                                                                                                                                  <  2:254278/198‑1 (MQ=255)                                                                                           ccGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCACCGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACAGTCACGACCTGGCTGTTGAGCCCCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAggtg                                                                                                                                           >  1:61714/1‑251 (MQ=255)                                                                                           ccGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAgg                                                                                                                                             <  2:217192/249‑1 (MQ=255)                                                                                           ccGCTGACTCCGTGTTCCAAATTGCCTGCCACGAAG‑GACCCTTTGGCCTGGATAAACTCTACGAACTGTGCTAAAGCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCATCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTTCTGCAGAAACTGGTTTATGAAAAACACGGTCAgg                                                                                                                                             <  2:441674/249‑1 (MQ=255)                                                                                             gCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTCCGACCTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAggtggt                                                                                                                                         <  1:512251/251‑1 (MQ=255)                                                                                             gCTGACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCCGTGACAAAGCCGGTAACTTTCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCCGCACCCACCGTGCTGCAGAAACTGGTTGATGACAAACACGGTCAggtggt                                                                                                                                         >  2:557375/1‑251 (MQ=255)                                                                                             gCTGACGCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGTATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACAAACGGCGGCTACAACATCGGTCGTGTTAACGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCATCACCGTCCGTGCTGCATAAACTGGTTGATGAAAAACACGGTCAggtggt                                                                                                                                         <  1:205520/251‑1 (MQ=255)                                                                                                gACTCCGTGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTc                                                                                                                                      <  1:139523/251‑1 (MQ=255)                                                                                                 aCTCCGGGTTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACACCATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCt                                                                                                                                     <  2:130787/251‑1 (MQ=255)                                                                                                      gtgtTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAAAATCGGTCGTGTTATCGATCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCgg                                                                                                                                >  1:560342/1‑251 (MQ=255)                                                                                                        gtTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑t                                                                                                                              <  1:402771/250‑1 (MQ=255)                                                                                                        gtTCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTAGCGCGCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTCCCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑t                                                                                                                              <  2:128811/250‑1 (MQ=255)                                                                                                          tCCAGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAAAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGCTGAAAAACACGGTAAGGTGGTTTCTGCCGG‑Taa                                                                                                                            >  1:360831/1‑250 (MQ=255)                                                                                                             aGATTGCCTGCCACGAAG‑GAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGG‑TAAAAtt                                                                                                                        >  1:390006/1‑251 (MQ=255)                                                                                                                   cctgccACGAAG‑GAACCTTGGGCCTGGATAAACTCTCCGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCATCGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGAc                                                                                                                  <  2:399168/251‑1 (MQ=255)                                                                                                                       gccacgaaggaaccgttggccTGGATAAACTCTACGAACTGGGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTCCCAGCGTACCGGTAACCGTCACGACCTGGCTGTGGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACAt                                                                                                                <  1:554644/230‑1 (MQ=255)                                                                                                                        ccacgaaggaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACACAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACAGACCGTGCTGCCGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑GAAAATTGCAGACAtat                                                                                                              >  1:53400/20‑249 (MQ=255)                                                                                                                         cacgaaggaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGG‑TAAAATTGCAGACATCt                                                                                                              >  1:6123/19‑250 (MQ=255)                                                                                                                         cacgaaggaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACctct                                                                                                              >  1:202734/19‑250 (MQ=255)                                                                                                                            gaaggaacctttggccTGGATAAACTCTACGAACTGTGCGAATTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGGGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGc                                                                                                          <  2:400060/236‑1 (MQ=255)                                                                                                                             aaggaacctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATAGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTCACAGTCACGACCTGGATGATGAGCCGCCAGCACCGACCGTGCTACAGAAACTGGTTGGTGAAAAACACGGTCAGGGGGTTTCTGTCGGATAAAAT‑GCAGACATCTACGcc                                                                                                         >  1:614944/15‑251 (MQ=255)                                                                                                                                        ggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACCTCGGCCGTGGTATCGCTCGTCCGGTTATCGGTGACAAAGCCGATAAATTCCACCGTACCGGTAACCGTCCCGCACTGGCTGTTGAGCCGCCAGCACCGCGCGTGCTGCTGAAACTGGTTGGTGAAAAAAACGGACCGGGGGGTTCTGTCGG‑TAAAATTGTAGACATCTACGCCCACTGCGGTAt                                                                                              >  2:534319/4‑251 (MQ=255)                                                                                                                                            tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCAcc                                                                                          >  1:457224/1‑251 (MQ=255)                                                                                                                                             ggATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGTTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCAc                                                                                           <  2:308234/249‑1 (MQ=255)                                                                                                                                               aTAAACTCTACGAACTTTGCGAAATCGCCCGCGAAGAGCTGACCAACGGCGGCTAAAACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGCCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaag                                                                                       <  2:539168/251‑2 (MQ=255)                                                                                                                                                tAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCa                                                                                         >  1:345695/1‑248 (MQ=255)                                                                                                                                                 aaaCTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTTGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaagaa                                                                                     <  1:264645/251‑4 (MQ=255)                                                                                                                                                  aaCTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaagaaa                                                                                    <  1:420927/251‑1 (MQ=255)                                                                                                                                                      ctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTTTCGGTGACCAAACCGGTAACTTCCCGCGTACCGGTAACCGTCACGACCTGGCTTTTGAGCCGCCAGCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCCGACCTCTACACCAACTGCGGTATCACCaagcaaatg                                                                                 >  2:55999/1‑243 (MQ=255)                                                                                                                                                          gAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCTCCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGc                                                                            <  1:145338/251‑1 (MQ=255)                                                                                                                                                               gggCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTg                                                                       <  2:173186/249‑1 (MQ=255)                                                                                                                                                                   gAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCt                                                                   <  2:115780/251‑1 (MQ=255)                                                                                                                                                                   gAAATCGCCCGTGAAGAGCTGACAAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTTCAGCGTACCGGTAACCGTCACGACCTGGCTGGTGAGCCGCCAGCACCGAACGGGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGTCGG‑TAAAATTGCAGACATTTACGCCAACTGCGGTATAACCAAGAAAGTGAAAGCAACTGGCCt                                                                   >  1:184498/1‑251 (MQ=255)                                                                                                                                                                      agcGCCCGTGAAGAGCTGCCCAACGGCGGCTACAACAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGa                                                                <  2:544973/249‑1 (MQ=255)                                                                                                                                                                              ggAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGt                                                        <  2:156083/250‑1 (MQ=255)                                                                                                                                                                                   agCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCCGGGGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACTGGCCTGGCCGCGCTGTTTGAc                                                   >  2:38504/1‑251 (MQ=255)                                                                                                                                                                                     cTGACCAACGGCGGCTCCAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGAGAAAGCAACTGGCCTGGACGCGCTGTTTGACGc                                                 >  2:73192/1‑251 (MQ=255)                                                                                                                                                                                       gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTCTCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGACGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcc                                                >  1:61037/1‑250 (MQ=255)                                                                                                                                                                                              ggcggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCa                                         >  2:487191/1‑250 (MQ=255)                                                                                                                                                                                                cggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAag                                      <  1:561349/251‑1 (MQ=255)                                                                                                                                                                                                 ggcTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAaga                                     <  1:603907/251‑1 (MQ=255)                                                                                                                                                                                                   cTACAACATCGGTCGGGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGGACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCCCCGACCGTGCTGCAGACACTGGTTGATGAAACACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCCAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCaacg                                      >  2:226973/1‑246 (MQ=255)                                                                                                                                                                                                    tACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAag                                      >  1:569303/1‑247 (MQ=255)                                                                                                                                                                                                     acaacaTCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGAt                                  <  2:243332/250‑1 (MQ=255)                                                                                                                                                                                                        acatCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAATCCGTGAACTTCCAGCGTACCGTTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGTTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACGGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACTCGCTGTTGGACGACATCATCAAAGAGTTGaaa                              <  2:614944/249‑1 (MQ=255)                                                                                                                                                                                                            cGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAaagaa                           <  2:546333/250‑1 (MQ=255)                                                                                                                                                                                                                   gtTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCGAACTGCGGTATCACCTAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGa                   <  1:568892/251‑1 (MQ=255)                                                                                                                                                                                                                      aTCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCGCCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATa                 <  1:229142/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAac               >  2:235811/1‑251 (MQ=255)                                                                                                                                                                                                                       tCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGATAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAac               >  1:540357/1‑251 (MQ=255)                                                                                                                                                                                                                         ccTCGTCCGTTTATCGGTGACACAGCCGGTAACTTCCAGCGTACCGGCAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGCAAAACACGGTCAGGCGGTTTCTGTCGG‑TAACATTGCAGACATCTACCCCAACTGCGGCATCACCAAGAACGTGAACGCAACTGCCCTGGACGCGCTGCTTGACGCCACCATCAAAGAGATGAACGAAGCgggcgctacccc             >  2:508567/2‑242 (MQ=255)                                                                                                                                                                                                                               ccGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGt       <  1:487191/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTTTATCGGTGTCAAAGCCGGTAACTTCCAGCGGACCGGTAACCGGCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGGCTG‑TAAAATTGCAGACATCTACGCCATCTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCt     <  1:570875/251‑1 (MQ=255)                                                                                                                                                                                                                                    tATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGACGCCCGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGG‑TAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGAAGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCCTCGTCTTcc  >  2:561891/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      ACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAG‑AAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGG‑TAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCA  >  NC_000913/4620024‑4620500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑