Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,442	T→C	L70P (CTA→CCA)	yaaX →	DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,442	0	T	C	100.0%	136.6 / NA	50	L70P (CTA→CCA)	yaaX	DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/21);  total (29/21)

GGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTA  >  NC_000913/5210‑5685                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gggCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAAGTGCGAGGCAAT‑CGCTGGCACCCCCACGGACCGCCGCCAcc                                                                                                                                                                                                                                      >  2:147303/1‑251 (MQ=255)    aaaTGATAAAAGGACTAACCTATGAAAAAGATGCAATCTATCGTACTAGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGCCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGCTAGGAGAGGCAAT‑CGCTGGCAACCACACGGACCGCCGCCAccgcc                                                                                                                                                                                                                                   >  2:572012/2‑251 (MQ=255)                      cctatgcaaaaGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGCATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCCGGCACCCACACGGCCCGCCCCCACCGCCGCGCC‑ACCATAAGAGAGc                                                                                                                                                                                                                >  2:302680/8‑251 (MQ=255)                        tatgAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGGC‑ACCATAAGAAAGctc                                                                                                                                                                                                              >  2:162828/3‑251 (MQ=255)                          tgAAAAAGATGCAATCTATAGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCAATGGCAGCACAGGCTGCGGAAATTACGTTATTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTCTGAATCGCGAGGCAATACG‑TGGCCCCCAAACGGCCCGCCCCCACCGCCGCGCCCACC‑TATGAAAGctcct                                                                                                                                                                                                            >  2:521276/1‑251 (MQ=255)                           gAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCTCCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGctcct                                                                                                                                                                                                            <  1:87016/250‑1 (MQ=255)                            aaaaaGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑CCCATAAGAAAGctcctc                                                                                                                                                                                                           >  2:366763/1‑250 (MQ=255)                             aaaaGATGCAATCTATCGTACTCGCACTTTCCCTGTTTCTGGTCGCTCCCATGGCATCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGTTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCAt                                                                                                                                                                                                         <  1:402884/251‑1 (MQ=255)                                  aTGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTCCGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGatca                                                                                                                                                                                                    <  1:473673/251‑1 (MQ=255)                                     cAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCAGCGCC‑ACCATAAGAAAGCTCCTCATGatcatcc                                                                                                                                                                                                 >  1:559210/1‑250 (MQ=255)                                       aacTATCGTCCCCGCACTTTCCCTGGTTCGGGTCGCTCCCATGGCAGCACAGGTTGCGGAAATTACGTTAGTCCCGTCAGAAAAATTACAGAGAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCAcg                                                                                                                                                                                               <  2:448274/249‑1 (MQ=255)                                        tCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCAcgg                                                                                                                                                                                              >  2:344895/1‑251 (MQ=255)                                             cGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTc                                                                                                                                                                                         >  1:568926/1‑251 (MQ=255)                                                aCTCGCCCTTTCCCGGGTGCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCAt                                                                                                                                                                                       <  2:329905/250‑1 (MQ=255)                                                    gCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACAAGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCCTGACCATCAcggcggcccgggccc                                                                                                                                                                                  >  2:411276/1‑242 (MQ=255)                                                       cTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGCCCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCAcggcgggcccggtccc                                                                                                                                                                                 >  2:344232/1‑239 (MQ=255)                                                         ttCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATCGTCCCGGc                                                                                                                                                                              >  1:93906/1‑250 (MQ=255)                                                                 ttttgggcgtccccaggacagcaCAGGCTGCGGAAATTACGTTAGTCCCTTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACGGGCGCGACCACGGCTGTTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGTTCATGGTCCAGGCAAACATCa                                                                                                                                                                      <  2:487551/232‑1 (MQ=255)                                                                 ttCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGCTCATCACGGCGGTccagggcccggcaaaaaaaac                                                                                                                                                                     >  2:478938/1‑231 (MQ=255)                                                                     ggTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCCCCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCg                                                                                                                                                                   <  1:12607/249‑1 (MQ=255)                                                                      gTCGCTCCCATGACAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCCCCCACCCGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTa                                                                                                                                                                <  2:329642/251‑1 (MQ=255)                                                                               atggcgccacaGGCTGCGGAAATTACGTTAGTCCCGGCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCTACCACGGCTGGTGGAAACACCATTATTAATGGCTAGGCAAT‑CTCTGGGACCCACACGGACCGCCGCCACCGCCGCGCC‑TCCATAAGAAAGCTCCTCATGatcat                                                                                                                                                                                                   <  2:298988/200‑1 (MQ=255)                                                                               aTGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGatcat                                                                                                                                                                                                   >  1:298988/1‑207 (MQ=255)                                                                                  gcagcaCAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGc                                                                                                                                                    <  1:49153/251‑1 (MQ=255)                                                                                        caGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCGGGTGGAAACAACATTAGGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTa                                                                                                                                              <  2:329579/251‑1 (MQ=255)                                                                                              gCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGACATGGTCCCGGCACACCACACCGCTaacagaccaaatccggggaaaaatc                                                                                                                                        >  1:601037/1‑228 (MQ=255)                                                                                                    aTTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTCCTGGGTTGGAGGTCCCTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACCCGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑At                                                                                                                                 <  1:55675/251‑1 (MQ=255)                                                                                                      tACGTTAGTCC‑GTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCCCGGCGGACATGGTCCAAGCAAACCTCACCCCCAAATAAAAAACGCCGGGTaaaaaacccgcaatac                                                                                                                               >  2:458069/1‑237 (MQ=255)                                                                                                                ccGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGAt                                                                                                                     <  1:130708/251‑1 (MQ=255)                                                                                                                        aaaaTTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGGAACAATCCGGC‑CTTCAGCGCCCGATGCGACGCt                                                                                                             >  1:170605/1‑251 (MQ=255)                                                                                                                                  aTAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCACTGTCCAAGCAAACATCCCCCCTAAAAGACCAATGCCGGGGAACAATCCGGC‑CTTACGCGCCCGATGCTAAGCTGGCGCGTCtt                                                                                                   >  2:198795/1‑251 (MQ=255)                                                                                                                                        gATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGTCCAGGCAAACATTACCCCTAACTGCCAAATGGCGGGGAACAATTCTGC‑ATTTAAGGCCCGATGCAACGCTGgcgtcgtttatta                                                                                               >  2:445762/1‑239 (MQ=255)                                                                                                                                              gATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGCCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACg                                                                                      <  1:181712/251‑1 (MQ=255)                                                                                                                                              aaaaagcgtgtgtATTACGGGGAGGGAGTCCACTGCGGCGACAACGGCTGGTGGAAATAACATTATGAATGGCGAGGCAAT‑CGCTGGCCCCCACACGGCCCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACg                                                                                      <  2:537329/239‑1 (MQ=255)                                                                                                                                                aaatctgggCTATGACTGGGATGGAGGTCACGGGCGCGCCCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGtg                                                                                    <  2:583388/244‑2 (MQ=255)                                                                                                                                                    cGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAAAATCACCGCTAAATGACAAAAGCCGGGGAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGtggatt                                                                                >  1:543858/1‑246 (MQ=255)                                                                                                                                                              aCTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAatat                                                                       >  1:117925/1‑250 (MQ=255)                                                                                                                                                                  ggATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTCTGAATGGCGAGGCAAT‑CGCTGGCACACACACGGACCGCCGCCTCCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCAGGCACAAATACCCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTAAGCGCCTGATGCGACGCTGGCGCGTCCTAAC‑CGGACCACGTGTATTCTGCAATATTTTg                                                                   >  1:386087/1‑250 (MQ=255)                                                                                                                                                                          gTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGCTCATCACGGCGGACATGGTCCAGGCAAACCTCAACGATAAATGACCAATACCGGGGAACAATCCCGC‑ATTCAGCGCACGGTGCGAAGCTGGCGCGTCTTCTC‑AGGCCTACGCGAATTCTGCAATATATTTTATCTGCAt                                                          >  1:375152/1‑251 (MQ=255)                                                                                                                                                                                                 tggAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑CTTCAACGCCCGATGCGACGCTGGGGCGTCTTATC‑AGGCCTACGTGAATTCTGCCATATATTTAATCTGCATGCTTTTGTAGGCCGGATAAGGCg                                   >  1:138324/1‑251 (MQ=255)                                                                                                                                                                                                 gggAAACAACATTATGAAGGGCGAGGCAAC‑CGCTGCCACCCACCCGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTGATC‑AGGCTTACGGGAAGTCGGCAATATATTGAATCTGCATGCTTGTGTAGGCCGGAGAAGGCg                                   <  2:93906/250‑1 (MQ=255)                                                                                                                                                                                                    aaaCACCATTATGAAGGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTc                                <  1:179172/251‑1 (MQ=255)                                                                                                                                                                                                               tGAATGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGGCCAAGCAAAAAACACAGCTAAATGACAAAAGTCGGGCAACAATCCGGCCATT‑AGCCCCTGATGCGACGCTGGCGCGGCTTATCCAGGCTAAGGGCAATTCTG‑AATATATTGAATCTGCATGGTTTTGTAGGACGGGTAAGGAGTTCACGCCGCacac                     >  2:176286/1‑248 (MQ=255)                                                                                                                                                                                                                gAATGGCCAGGCAAC‑CGCTGCCACCCACCCGGCCCGCCGCCCCCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                    <  2:550629/251‑1 (MQ=39)                                                                                                                                                                                                                   tGGCGAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTTAATCTGCATGCTTTTTTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGc                  >  1:149492/1‑250 (MQ=255)                                                                                                                                                                                                                       gAGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCCGGCAAACATCACCGCTAAATGACAAAAGCCGGGGAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGGATTTGCATGCTTTTTTAGGCCGGATAAGGCGGTCCCGCCGCCTCCGGCAttt              >  1:182571/1‑249 (MQ=255)                                                                                                                                                                                                                        aGGCAAT‑CGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGAc            <  1:286175/251‑1 (MQ=255)                                                                                                                                                                                                                           cAAT‑GGCTGGCCCCCACCCGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCCCGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGc         <  1:478938/251‑1 (MQ=38)                                                                                                                                                                                                                                 gCTGGCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGCTCATCACGGCGGACCTGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGGAACAATCCGGC‑ATTAAGCGCCTGCTGCCACGCTGGCGCGTCTTATC‑AAGCCTCCGTGAATTCTTCCAAATATTGAATCTGCATGCTTTTGTTGGCCGGATAAGGCGTTTACGCCCCCTCCGGCCTTGACTGCCAACt    >  2:283693/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggCACCCACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTa  >  1:43710/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAAT‑CGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCC‑ACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGC‑ATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATC‑AGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTA  >  NC_000913/5210‑5685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑