Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,080	G→T	L284L (CTG→CTT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,080	0	G	T	98.9%	277.1 / NA	88	L284L (CTG→CTT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (39/48);  total (40/48)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑GGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG  >  NC_000913/4618834‑4619321                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTAtct                                                                                                                                                                                                                                                >  1:530473/1‑251 (MQ=255) cGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTAtct                                                                                                                                                                                                                                                <  2:530473/251‑1 (MQ=255)  gTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTAtctc                                                                                                                                                                                                                                               <  1:459372/251‑1 (MQ=255)   ggAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGTTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTTAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTTGGTGATGTCACGATGGCGCTGGGCGGGGAGATGTTTATCTcc                                                                                                                                                                                                                                              <  2:591890/250‑1 (MQ=255)    gAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACTATGGCGCTGTGCGTGGAGATGCTTATCTCCg                                                                                                                                                                                                                                             <  1:536594/251‑1 (MQ=255)      aaGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGc                                                                                                                                                                                                                                           >  1:477577/1‑251 (MQ=255)      aaGTGGGTAGCGGCGCGTCTATGCCGACCTCCGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCCCCACCGCGCTGCTCACCGACATAAATCAGGGACTGGCCTCCCGTGCAGGTAACGCGGTTGCAGTTCGTGAAGCGGGGCAGGTCCTGACGGGGGAGTATAGTAACCCGCGGCTGTTTGCGGTCCCGATGGCGATGTGCGGGGAGATGCTGAGCTCCGGc                                                                                                                                                                                                                                           >  2:2064/1‑251 (MQ=255)        gTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCaa                                                                                                                                                                                                                                         <  1:509016/251‑1 (MQ=255)           ggTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGCAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGACTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGGGGAGATGCTTATCTCCGGCAAACt                                                                                                                                                                                                                                      >  2:504908/1‑251 (MQ=255)           ggTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACt                                                                                                                                                                                                                                      >  1:133835/1‑251 (MQ=255)                 ggcgCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGa                                                                                                                                                                                                                                 >  1:190454/1‑250 (MQ=255)                  gcggcttgaTGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGTCAGGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTGGAATTGCGTGAAGCGGTGCTGTTCCTGACGGTTGAGTATCGTAACCCGCTTCTGTTTGATGTCACGATGGCGCTGTGCGTGGTGATGCTTAGCTCCGTCAAACTGGCGaaa                                                                                                                                                                                                                               <  2:372847/243‑1 (MQ=255)                    gcgTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGaa                                                                                                                                                                                                                                >  2:108980/1‑248 (MQ=255)                       tttATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATg                                                                                                                                                                                                                           >  2:339168/1‑250 (MQ=255)                        ttATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGa                                                                                                                                                                                                                          <  1:419566/250‑1 (MQ=255)                        ttATGCCGACCTACGAACTCGCTGAAGCCCGTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCGCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGa                                                                                                                                                                                                                          <  2:95857/250‑1 (MQ=255)                            gcctaCCTACGAACGCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGGCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGcc                                                                                                                                                                                                                      <  2:172763/246‑1 (MQ=255)                               gACCTACGCACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGCGGAGATGCTTATCTCCGGCACACTGGCGAAAGATGACGCCGa                                                                                                                                                                                                                    >  2:77422/1‑249 (MQ=255)                                    tcGAACTCTCTGAAGCCCTTGCCGAAGCGATTGGTGGCGGGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGGTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGGGCGTGGAGAGGCTTATCTCCGGGAAACTGGCGAAAGATGACGCCGAAgcgc                                                                                                                                                                                                               <  2:48214/248‑1 (MQ=255)                                      gAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcgcgc                                                                                                                                                                                                           >  2:223451/1‑251 (MQ=255)                                       aaCTCTCTGAAGCCCGTGCCGAAGCGAGTGGTGGCGTGGCTAACGGCGCGGGCGTGCGCACCACCGCGCGGCTGACCGACATGAATCAGGGACGGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTTTTGAAGCGGGGCAGTTGCTGACGGGTGAGTATCGTAACCCGCGTCTGGTTGATGTCAGGATGGCGCTGTGCGTGGATATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGcgcgcgcg                                                                                                                                                                                                          <  1:621855/251‑1 (MQ=255)                                          tctctGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCCGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCCCGATGGCGCTGTGCGTGGAGATTCTTATCTCCGGAAAACTGGCGAAATATGACGCCGAAGCGCGCGCGaa                                                                                                                                                                                                        >  2:488618/1‑250 (MQ=255)                                              tGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGc                                                                                                                                                                                                   >  1:515860/1‑251 (MQ=255)                                               gAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCa                                                                                                                                                                                                  <  1:156873/251‑1 (MQ=255)                                                gtgCCCTTGCCGAGTCGATCGTTGGCGTGGCTGTCGGAGTGGGCGTGCGCACTACCCCCCTGCTACCCGACATGAAGCATGTACTGGCCTCCAGTGCAGGTAACGCGTTTGAAGGTCGTGAAGCGGTGCATTTCCTGACGGGTGAGGATCGTTACCCGCGTCTGTTTGATGTCACGATGGCGCGGTGCGGTGAGATGCTTATCTCCTGCAAACTGGCTAAAGATGActattaagtgcgcgcgacatttct                                                                                                                                                                                                  <  2:367100/248‑24 (MQ=255)                                                   cccTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCaagca                                                                                                                                                                                              <  1:223451/251‑5 (MQ=255)                                                       tGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGAAGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGc                                                                                                                                                                                         >  1:229651/1‑251 (MQ=255)                                                          cGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCAAGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCCGGTAACGCGGTTGAAGTTCGTGACGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGCTGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTgg                                                                                                                                                                                      >  1:411657/1‑251 (MQ=255)                                                           gAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGACGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTgg                                                                                                                                                                                      >  1:83665/1‑250 (MQ=255)                                                           gAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTgg                                                                                                                                                                                      >  1:46069/1‑250 (MQ=255)                                                               agATTGTTGGCGTGGCTAACGGCGCTGGCGTGAGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGACAGATGACGCAGAAGCGCGCGCGCAATTGCA‑AGCATGGCTGGACAAc                                                                                                                                                                                 >  2:332586/2‑251 (MQ=255)                                                                gATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACg                                                                                                                                                                                <  1:347686/251‑1 (MQ=255)                                                                   tgttgGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGATGCTCCCCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGAGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGt                                                                                                                                                                              >  2:553892/1‑250 (MQ=255)                                                                      tgGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTaaa                                                                                                                                                                           <  1:507785/250‑1 (MQ=255)                                                                        gCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCg                                                                                                                                                                        <  2:167158/251‑1 (MQ=255)                                                                         cGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCgg                                                                                                                                                                       <  1:553892/251‑1 (MQ=255)                                                                                aaCGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAg                                                                                                                                                                 <  1:548592/250‑1 (MQ=255)                                                                                aaCGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGt                                                                                                                                                                <  1:356066/251‑1 (MQ=255)                                                                                 aCGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGCAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGaagaaggc                                                                                                                                                               >  2:222720/1‑249 (MQ=255)                                                                                  cGGCGCTGGCGTGCGCACCAACGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGACGCGGAGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGATGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAACGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCCGTGCTGGACAACGGGAAAGCGGCAGACGTCt                                                                                                                                                              >  2:542879/1‑251 (MQ=255)                                                                                      gcTGGCGTGCGAAACACCGAGCTGCTCACAGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGATGAAGTTCGTGAAGCGGTGCCGTTCCTGGCGGGTGCGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGGGCGTGGAGATGCTTATCTACGGCAAACTGGCGCAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGAAGAAGTATTTgg                                                                                                                                                          >  2:558002/1‑251 (MQ=255)                                                                                       cTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGCTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTTGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTCGGt                                                                                                                                                         >  1:108740/1‑251 (MQ=255)                                                                                                        gcgcTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGcaca                                                                                                                                        <  2:241846/251‑1 (MQ=255)                                                                                                                 aCCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGATGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGAAGCGGGACAAAAAAGccc                                                                                                                               >  1:59242/1‑251 (MQ=255)                                                                                                                        tGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGGTCGTGAAGCGGTGCAGTTCATGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGATGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGCCGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTAGGGGAGCGGCACaaaacagccccacggac                                                                                                                        >  2:195975/1‑238 (MQ=255)                                                                                                                               ggTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGGTCGTGAAGCGGTGCAGTTCCTGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTg                                                                                                                 <  2:229651/251‑1 (MQ=255)                                                                                                                                  ccTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCGGACGGGTGAGTATCGTAACCCGCGTCTGGTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTgaga                                                                                                              <  2:352220/250‑1 (MQ=255)                                                                                                                                  aCTGGCCTCCAGTGCAGGTAACGCCGTTGAAGTTCGTGACGCGGTCCAGTTCATGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCGCGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGCAAGCTGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGAATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTg                                                                                                                 >  2:618561/1‑248 (MQ=255)                                                                                                                                    tGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGCTGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAAc                                                                                                            >  2:42293/1‑251 (MQ=255)                                                                                                                                                         cgGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCTTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACa                                                                                       <  1:431748/251‑1 (MQ=255)                                                                                                                                                          ggTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGAc                                                                                        >  1:450275/1‑249 (MQ=255)                                                                                                                                                          ggTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGAc                                                                                        >  1:541385/1‑249 (MQ=255)                                                                                                                                                              gAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGAt                                                                                  <  1:108980/251‑1 (MQ=255)                                                                                                                                                                   tCGTGAAGCGGTGCAGTTCTTGACGTGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGGGTGGAGATGCGTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAAGCGATTTCGTTGGGAACTACGCGAAGTATCTGCCCACAGCGATGCTg                                                                              <  2:429027/250‑1 (MQ=255)                                                                                                                                                                    cGTGAAGCGGTGCAGTTCCTGACGGTTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGTTTTCGTTGAGAACTACGCGAAGTAGCTGCCGACAGCGATGCTGAc                                                                            <  1:503959/251‑1 (MQ=255)                                                                                                                                                                    cGTGAAGCGGTGCAGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGCAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGAc                                                                            >  1:257609/1‑251 (MQ=255)                                                                                                                                                                    agTGAAGCGGTGCAGTTCCTGAAGGGTGAGTCTCGTAACACGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAAAGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGAACATAAAGGCCCAACCGACTTCGTTGAGAACTCAGCGAAGTATAGACCGACAGCGATGCTGa                                                                             >  2:416431/2‑250 (MQ=255)                                                                                                                                                                                 aGTTCCTGACGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTAt                                                               >  1:351969/1‑251 (MQ=255)                                                                                                                                                                                     ccTGACGGGGGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAATATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGTCTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTg                                                           <  2:492749/251‑1 (MQ=255)                                                                                                                                                                                         gcGGGTGAGTGTCGTAACCCGCGGCTGTGTGATGTCACGATGGCGCTGTGCGGGGAGATGCGTAGCTCCGGTAAAGTGGCGAAAGATGACGGCGAAGCGCGCGCGAAATGGCAAGGAGG‑GTTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTTGTATGGTAGCTGCACAAAAAGGCCCAAGCGTCTTCGTTGAGAACTGCGCGAAGTATCGGCCGACAGTGAGGCTGTCGAAAGCACTCTTTGCTGATAc                                                       <  2:568568/250‑1 (MQ=255)                                                                                                                                                                                          cGGGTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATAcc                                                      <  2:83665/251‑1 (MQ=255)                                                                                                                                                                                           gggTGAGTAGCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGGCTTTGGTCGTATGGTTGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATGTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCg                                                     <  1:266720/251‑1 (MQ=255)                                                                                                                                                                                            ggTGAGTATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGa                                                    <  1:432227/251‑1 (MQ=255)                                                                                                                                                                                               gattatCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAg                                                  <  2:254860/247‑1 (MQ=255)                                                                                                                                                                                                      gTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTc                                          >  1:482498/1‑251 (MQ=255)                                                                                                                                                                                                           ccGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTg                                      >  2:616974/1‑250 (MQ=255)                                                                                                                                                                                                            cgcgTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGTCGAAGCTCGCGCGAAATTGCA‑TGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCATCCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATTCTGATACCGAAGGTTTTGTTAGTGaa                                    <  2:123000/251‑1 (MQ=255)                                                                                                                                                                                                             gcgTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGaaa                                   <  2:153408/251‑1 (MQ=255)                                                                                                                                                                                                             gcgTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGaaa                                   <  1:190327/251‑1 (MQ=255)                                                                                                                                                                                                                 cggTTTGATGTCTCGATGGCGCGGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAAGTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTGTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGTTGATACCGAAGGTTTTGTCAGTGAAATgg                                <  1:209565/248‑1 (MQ=255)                                                                                                                                                                                                                   gTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAt                              >  1:600478/1‑250 (MQ=255)                                                                                                                                                                                                                   gTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTAGGCTGATACCGAAGGTTTTGTCAGGGAAATgg                                <  1:162722/248‑1 (MQ=255)                                                                                                                                                                                                                      tGATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGTCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAccc                          >  1:532582/1‑251 (MQ=255)                                                                                                                                                                                                                       gATGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAccc                          <  1:540913/250‑1 (MQ=255)                                                                                                                                                                                                                        aTGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTCAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCcgc                        >  2:39987/1‑251 (MQ=255)                                                                                                                                                                                                                         tGTCACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCcgcg                       >  2:503947/1‑251 (MQ=255)                                                                                                                                                                                                                            cACGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCcgcgcg                     <  1:542879/250‑1 (MQ=255)                                                                                                                                                                                                                             aCGATGGCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCt                   <  1:488618/251‑1 (MQ=255)                                                                                                                                                                                                                               gagtgcgcTGTGCGTGGAGATGCTTATCTCCGGCAACCTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGGCGTATGTTAGCGGCACAAAAAGGCCCAACCGTCTTCGTTGAGAACGACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCTGTCTATGCTGATACCTAAGGTTTTGTCAGTGAAATGGATACCCCCGCGCTgg                 <  2:368932/247‑1 (MQ=255)                                                                                                                                                                                                                                  ggCGCTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTgggg               <  1:77422/250‑1 (MQ=255)                                                                                                                                                                                                                                    cgcTGTGCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGGCGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGTTAAAGCGGCAGAAGTCTTTGGGCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATg            <  1:359188/251‑1 (MQ=255)                                                                                                                                                                                                                                       tgtgCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTg                                                                              >  2:295829/1‑182 (MQ=255)                                                                                                                                                                                                                                       tgtgCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTg                                                                              <  1:295829/182‑1 (MQ=255)                                                                                                                                                                                                                                         ggCGTGGAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGt       <  2:482498/250‑1 (MQ=255)                                                                                                                                                                                                                                            gTGGAGATGCTTATCTACGGCAAACTGGCGAGAGATGACGGCGAAGCGCGCGAGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGACTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGACGACAGCGATGCTGCCGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATAACCGCGCGCTGGGGATGGCAGTGGt    >  2:400796/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGTGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAATGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg  <  1:630110/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggAGATGCTTATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTg  >  2:9074/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggAGATGCTTAGCTCCGGCAAACTGGCGAAAGAGGACGCCGAAGCGTGCGCGAAATTGCA‑AGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACGACGCGAAGGATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTGGTCATTGAAATGGATACCCGCTCGCTGGGGATGGCAGTGGTTg  <  2:434436/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGAACTCTCTGAAGCCCTTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCA‑GGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTG  >  NC_000913/4618834‑4619321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑