Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,489	C→T	T87T (ACC→ACT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,489	0	C	T	98.8%	275.8 / NA	83	T87T (ACC→ACT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/35);  minor base G (0/1);  total (47/36)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTACGCG  >  NC_000913/4618247‑4618735                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         attattCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGt                                                                                                                                                                                                                                                  >  1:204571/1‑251 (MQ=255)        gTAAACAACGTGCTGGTCATGCACTGAGCGATTAAGAACTTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGACGTGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGCTATGACAATGCCTGAGCGTGTCTCGC‑TGTCCCTGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAACAGCCTGCATCTGACTGGCCCGATTTTTGATCACCATTCCACTGGTGGCGTCGGCGAt                                                                                                                                                                                                                                           >  2:355238/1‑251 (MQ=255)        gTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGAt                                                                                                                                                                                                                                           >  2:250603/1‑251 (MQ=255)          aaaaaaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGAtgt                                                                                                                                                                                                                                         <  1:303528/251‑1 (MQ=255)              aaCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACt                                                                                                                                                                                                                                     >  1:163051/1‑251 (MQ=255)               aCGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACtt                                                                                                                                                                                                                                    >  1:159040/1‑251 (MQ=255)                   gATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGACTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGACAAGCCTGCATCTGAATGGCCCGATTGTTGATCAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCt                                                                                                                                                                                                                                >  1:421556/1‑251 (MQ=255)                       gTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATg                                                                                                                                                                                                                            >  1:390690/1‑251 (MQ=255)                         cATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGtt                                                                                                                                                                                                                          <  1:128833/251‑1 (MQ=255)                            gcacTGAGCGATGAAGAAAATCGTTTCTTTATCAACGGCATTCGCGACAACCCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGAGATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTggg                                                                                                                                                                                                                       <  2:247987/248‑1 (MQ=255)                                 gAGCGATGAAGAAAGTCGTTTATTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TAATCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGcc                                                                                                                                                                                                                    <  2:495803/249‑1 (MQ=255)                                   gCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATg                                                                                                                                                                                                                <  1:349342/251‑1 (MQ=255)                                        gaagaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCg                                                                                                                                                                                                            <  1:250603/250‑1 (MQ=255)                                                   tttCTTTATCAACGGCATTAGAGCCAACACTATCTACGAAGGGCAGATTGCCGCCCTCGCGCTGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTAGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCCGCCTgcggcg                                                                                                                                                                                                >  2:223892/1‑251 (MQ=255)                                                      cTTTATCCACGGCATTCGCGACAACACTATCTCCGTCGGGCAGATTGCCGTCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATGTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTAGCTGATGTTGGGGCCGCTGGTCGCAGCCTGCGGCGGCt                                                                                                                                                                                             >  2:61376/1‑251 (MQ=255)                                                        ttATCAACGGCATTCGCGACAACCCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCta                                                                                                                                                                                            <  2:451690/250‑1 (MQ=255)                                                              aCGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCg                                                                                                                                                                                     <  1:33630/251‑1 (MQ=255)                                                               cggcaTTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTAGCGATGACCATTTTCTTCCACGATATGACAATGACTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACAGTTCTCGACTGGAACAGACTGCATCTGAATGGCACGATTGTTGATAAACATTCCACTGGTGACGTCGGCGATGTGACTTCGCTGATGATGGGGCCGATGGTCGCAGCCTGCGGCGGCTATCTTCCg                                                                                                                                                                                     >  1:561783/5‑250 (MQ=255)                                                                   aatCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTCCCACGATATGACAATGCCTGATCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGGCGGCGATGTGACTTCGCTGAGGTTGTTGCCGATTGTCGCAGCCTGCGGCGGCTATATTCCtatgat                                                                                                                                                                                <  2:344460/249‑1 (MQ=255)                                                                   aTTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCgatgat                                                                                                                                                                                >  1:177233/1‑251 (MQ=255)                                                                         gACAACACTCTCTCAGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGAGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTgg                                                                                                                                                                          >  2:567280/1‑251 (MQ=255)                                                                         gACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGACCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGCCTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGGCATTTTCCCATGATCTCTgg                                                                                                                                                                          >  2:434005/1‑251 (MQ=255)                                                                            aacaCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTcg                                                                                                                                                                       >  2:545736/1‑251 (MQ=255)                                                                               acTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCgg                                                                                                                                                                    <  2:9598/251‑1 (MQ=255)                                                                               acTATCTCAGTAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGCACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCTCTGATGTTGGGGCCGATGGTAGCAGCCTGCGGCGGATATATTCCGCTGATCTCTGGCCGCgg                                                                                                                                                                    >  1:501948/1‑251 (MQ=255)                                                                                  atcgccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTGCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATGCCGAGGATCTCTGGTCGCGGc                                                                                                                                                                   <  2:210158/245‑1 (MQ=255)                                                                                  aTCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGccc                                                                                                                                                                 >  2:264980/1‑250 (MQ=255)                                                                                      ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTTCGATGATCTCTGGTCGCGGCCTCggg                                                                                                                                                             >  1:352230/1‑250 (MQ=255)                                                                                               aGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATTCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGc                                                                                                                                                    <  2:347265/251‑1 (MQ=255)                                                                                                gATTGCCGCCCTCGCGATGACCATTTTCTGCCACGATATGCCAATGCCTGCGCGTCGCTCGCGTG‑CCATGGCGATGCGAGATTCAGGAACCGTTCTCGTCTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCTATGTGACTTCGTTCATGTTGGGGCAGATGGTCGCAGCCTGCGGCGGCTATAGTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCg                                                                                                                                                   <  2:494623/251‑1 (MQ=255)                                                                                                    gccgccCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTAc                                                                                                                                               <  2:390690/251‑1 (MQ=255)                                                                                                          cTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTaagcccgg                                                                                                                                         >  2:160915/1‑244 (MQ=255)                                                                                                                  gACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTgg                                                                                                                                >  1:178080/1‑251 (MQ=255)                                                                                                                    ccATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGaa                                                                                                                              <  1:409533/251‑1 (MQ=255)                                                                                                                       ttttCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCCTGGCGATGCGAGCTTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCCCTGGTCGCGGCCTAGTTCATACTGGCGGGACGcctggctacccggggacc                                                                                                                            >  2:279164/1‑234 (MQ=255)                                                                                                                       ttttCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGTTGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGGTCTCTGGTCGCGGCCTCGGGCATACCGGCGGGACGCCCGACA‑AAcgtgcaccc                                                                                                                           >  2:18086/1‑243 (MQ=255)                                                                                                                          tcttcCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAatccat                                                                                                                         <  1:517063/250‑1 (MQ=255)                                                                                                                          tcttcCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGGCATACTGGCGGTACGCTCGACA‑AACTGGAatccatc                                                                                                                        >  1:184303/1‑251 (MQ=255)                                                                                                                             tcctctatttGACAATGTCTGAGCGTGTCTCGC‑TGACCATGTCGATGCGAGATTCAGGAACCGTTCTCGACGGGAAAAGCCTGCATCTGAAGTTCTCGAGTTTTGATAAACATTCCACTGGTGGCGTCGGCGATGGGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCTGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACGGGTatccagc                                                                                                                        <  2:480802/239‑3 (MQ=255)                                                                                                                                   atatGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTc                                                                                                               <  1:508811/251‑1 (MQ=255)                                                                                                                                     atGACAATGCCTGACCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCg                                                                                                              <  2:337579/250‑1 (MQ=255)                                                                                                                                          aaTGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGGCC‑AAATGGAATCCCTCCCCCGCTTCGGCAttt                                                                                                        >  1:352220/1‑251 (MQ=255)                                                                                                                                            tGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCCTCCCCGGCTTCGACAttttt                                                                                                      >  1:409884/1‑250 (MQ=255)                                                                                                                                                  ggCGTGTCTTGC‑TGACCATGGCGATGCTAGATTCAGGACCCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAACCATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCGCGGTCATACGGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGAt                                                                                                <  2:385898/250‑1 (MQ=255)                                                                                                                                                  aGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGCTGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTAACCTCGAAC‑AACTGGGATCCCTCCCGCGCTTCGACATTTTCCCGGAt                                                                                                >  1:76753/1‑251 (MQ=255)                                                                                                                                                         ctcGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAGAGCATGCATCTGAATGGCCCGATTGTTGATAAGCATTCCACTGGTGGCATCGGCGATATGACTTCGCTGCTGTTGGGGCCGATCGTCGCAGCCTGCGGCGGCTATATTACGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCTCGTCACAAC‑GGAATCCATCCCCGGCTTCGTAATTTTCCCGGGTGGCAAcc                                                                                         >  1:594820/1‑251 (MQ=255)                                                                                                                                                            gC‑TGACCATGGCGATGCGAGATTCAGGAAGCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAACCATTCCACTGGTTGCGTCGGCGAGGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCGCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGtt                                                                                      <  2:159408/251‑1 (MQ=255)                                                                                                                                                               tGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCCACA‑AACTGGGATCCATCCCTGGCTTCGACATTTTCCCGGATGAAAACCGTTTc                                                                                    >  1:189920/1‑251 (MQ=255)                                                                                                                                                                gACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGttt                                                                                     <  1:386164/249‑1 (MQ=255)                                                                                                                                                                gACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGttt                                                                                     <  2:184303/249‑1 (MQ=255)                                                                                                                                                                        cGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAatt                                                                           <  1:487111/251‑1 (MQ=255)                                                                                                                                                                          aTGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGGTGATCTCTCGGCGAGGCCTCGGACCTACGGGCGGTACGCTCGGCA‑AACCGGAATCCCTCCCTTGCCTCGGCCTTTTTCCGGGTGGCAACCCTTTcccgggacttat                                                                         >  2:489637/1‑241 (MQ=255)                                                                                                                                                                          aTGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCAGCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCGCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAattat                                                                         <  2:189920/251‑1 (MQ=255)                                                                                                                                                                           tGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCAACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGACTGCGGCGGCTATATTCCGATGATCTCTGGGCGCGGCCTCGGTCATACTGGCGGCACGCTCGACA‑ACATGGAATCCATCCCTGGCTACGACATTTTCCCGGATGCCAACCGtttacccccaattatt                                                                        >  1:562397/1‑238 (MQ=255)                                                                                                                                                                            gCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCCTCCCTGGCTTCGCCATTTTTCCGGGTGACAACCGTTTCCGCGGAattttt                                                                        >  2:179503/1‑247 (MQ=255)                                                                                                                                                                             cGAGATTCAGGAACAGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACC‑AACTGGAATCCCTCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAAttatta                                                                       >  1:172763/1‑250 (MQ=255)                                                                                                                                                                                 aTTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGAc                                                                  <  2:163051/251‑1 (MQ=255)                                                                                                                                                                                    cAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGGTCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACCCCCGGCA‑AACTGGAAACCCTCCCTGGGTTCGGCATTTTCCCGGATGGCAACCGTTTACGCGACattttttaaaaagtc                                                               >  2:214960/1‑239 (MQ=255)                                                                                                                                                                                     aGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCg                                                              <  2:7497/251‑1 (MQ=255)                                                                                                                                                                                       gAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCAGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGc                                                            <  1:609919/251‑1 (MQ=255)                                                                                                                                                                                           cGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCTCGTCA‑AACTGGAATCCATCCCTGGCTTCGACATTTTACCGGGTGGCAACCGTTTCCGCGACATTTTTAAAAACGTCGGCGTgg                                                        >  1:532048/1‑251 (MQ=255)                                                                                                                                                                                            gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGTTCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACGCTCGACA‑AACTGGAATCCAACCCTGGCCTCGACATTTTTCCGGTTGAAAAACGTTTCCCCGAAATTATTTAAGAAGTCGGCGTggg                                                       >  1:449416/1‑250 (MQ=255)                                                                                                                                                                                               atcGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGGCATACTGGGGGGATGCGCGACA‑AACTGGATTGCCTCCCTGGCTTCGACATTTTTCCGGATGACAACCGTTTCCGCGACATTTTTTAAGACGTCGGCGGGGCggg                                                    >  2:2032/2‑249 (MQ=255)                                                                                                                                                                                                     tGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTTTATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑CACTGGCATCCCTCCCTGGCTTCGACCTTTTCCCGGGTGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCgg                                              >  1:361745/1‑251 (MQ=255)                                                                                                                                                                                                      ggAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATAGTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCTCGAAATTATTAAAGACGTCGGCGTGGCGATTATCg                                               <  1:171309/249‑1 (MQ=255)                                                                                                                                                                                                       gAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTTTTTAAGACGTCGGCGTGGCGATTATCgggc                                            >  1:254860/1‑249 (MQ=255)                                                                                                                                                                                                             ccTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCCTACTGGCGGGACGCTCGACC‑AACTGGGATCCATCCCTGGCCTCGACATTTTTCCGGGTGACAACCCTTTCCGCGGAATTATTAAAGACGTCGGCGGGGCGGTTTTCgggtaaaccc                                      >  2:275169/1‑243 (MQ=255)                                                                                                                                                                                                               tGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTTGCGGCCTCGGTCCTACTGGCGGTACCCCTGGCA‑AAAAGGAATCCATCCCTCGCTTTGACACTTTCCCGGGTGGCCAAAGGTTCCCCGAAATTATTTAATACGGCGGCGGGGGGGTTATCgggctaaccccc                                    >  2:136846/1‑241 (MQ=255)                                                                                                                                                                                                               gtcATCTGAATGGCCCGATTGTTGAGAAACATGCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTAGATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCTGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAgc                                   <  2:501948/249‑2 (MQ=255)                                                                                                                                                                                                                 cATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAgctc                                 <  2:159040/251‑4 (MQ=255)                                                                                                                                                                                                                    cTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGCTGCTCTCTGGTCGCGGCCTCGGTCATACTGGCGGTAAGCCCGACA‑AACTGGAATCCCTCCCCCGTCTCGACATTTTCCCCGCTGGAAACCGGTTTCGCGAAAATTTTAAAAACCTCGGCCTGGCGATTATCGGG‑TAGACCaccccccc                              >  2:52124/1‑244 (MQ=255)                                                                                                                                                                                                                     tGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGAAGCTCGACA‑AACTGGAATCCATCCCCGGCTTCGACATTTTCCCGGATGACAAACGTTTCCGCGAAATTATTAAAGACGGCGGCGTGGCGATTATCGGTTCA‑ACCAGCTCACTg                             >  1:111713/1‑251 (MQ=255)                                                                                                                                                                                                                      gAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGCTCAc                               <  1:526620/248‑1 (MQ=255)                                                                                                                                                                                                                       aaTGGCCAGATTGTTGAAAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCATCGGTCATACTGGCGGGACGCTCGACC‑AACTGGAATCCATCCCCGGCATCGCCATTTTCCCGGGTGACAACAGTTTCCGCGAAATTATTTAAGAAGTCGGCGGGGGGATTATCGGC‑CAGAACAGCTCACTGGc                           >  1:447965/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGCTCACTGGCTcc                        <  2:178080/251‑1 (MQ=255)                                                                                                                                                                                                                            cccGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGCCATTTTTCCGGATGACAACCGTTTCCGCGAAATTATTTAAAACGTCGGCGTGGCGGTTATCGGT‑CAGAcccacccactggcgcccg                      >  2:8066/1‑235 (MQ=255)                                                                                                                                                                                                                               gATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑CACTGGAATCCATCCCCGGCTTCGGCATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAAACGTCGGCGGGGCGGTTTTCGGT‑CAGaaccactcacctgcccccgggg                   >  2:286996/1‑230 (MQ=255)                                                                                                                                                                                                                                aTTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AAATGGAATCCATCCCTGGCTTCGACATTTTCCCCGGTGACAACCGTTTCCCCGAAATTATTAAAGACGTCGGCGTTGCGGTTATCGGT‑CAGACCAGCCCACTgggcccggcggg                  >  2:493954/1‑241 (MQ=255)                                                                                                                                                                                                                                aTTGTTGATAAACAGTCCACTGGTGGCGTCGGCGATGTGACTGCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCGCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGCTCACTGGCTCCGGcgga                  <  2:204571/251‑4 (MQ=255)                                                                                                                                                                                                                                     tgATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGGAATTATTAAAGACGTCGGCGTGGCGATTATCGGG‑CAGACCCGCTCACTGGCTcccgcgggtaaac             >  1:155832/1‑240 (MQ=255)                                                                                                                                                                                                                                       aTAAACATTCCACGGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCGTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGCTCACTGGCTCCGGCGGATAAACGt           <  1:52124/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATCTTCCGATGATCTCTGGTCGCGGCCTCGGGCATACTGGCGGTACGCCCGACA‑AACTGGAATCCCTCCCTGGCTTCGGCATTTTTCCGGGAGACAACCGTTTCCCCGAAATTATTAAAGACGCCGGCGGGGCGATTTTCGGG‑CCGTCCACATCACCGGCTCCGGCGGATAAACGTTTCTAcgcg  >  2:156873/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGCTCACTGGCTCCGGCGGATAAACGTTTCTAcgc   <  1:545736/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         ATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGC‑TGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACA‑AACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACCGTTTCCGCGAAATTATTAAAGACGTCGGCGTGGCGATTATCGGT‑CAGACCAGTTCACTGGCTCCGGCTGATAAACGTTTCTACGCG  >  NC_000913/4618247‑4618735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑