Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,234	G→A	M1M (GTG→ATG) †	yaaX →	DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,234	0	G	A	100.0%	150.9 / NA	48	M1M (GTG→ATG) †	yaaX	DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/18);  total (30/18)

GCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCC  >  NC_000913/4993‑5463                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      gcgtAAATTGAGGAGGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAATAACGACAGGGAAAAAGGAGAAAGTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTGCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGAt                                                                                                                                                                                                                             <  1:36974/251‑1 (MQ=255)   gtAAATTGATGATGAATAATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGc                                                                                                                                                                                                                           >  2:87016/1‑251 (MQ=255)     aaaTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCa                                                                                                                                                                                                                          >  1:346485/1‑250 (MQ=255)          gatgatgaATCATCAGTAAAAACTATTCATTATCTCAATCAGGCCGGGTTTGCTTGTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCAACGCGTAATCTCCGGATAACGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATcga                                                                                                                                                                                                                     <  2:525317/250‑3 (MQ=255)             gatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCg                                                                                                                                                                                                                  <  1:400548/250‑1 (MQ=255)                gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTAc                                                                                                                                                                                                               <  1:164638/250‑1 (MQ=255)                    catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCa                                                                                                                                                                                                          <  2:166611/251‑1 (MQ=255)                       caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACtt                                                                                                                                                                                                       <  2:2479/251‑1 (MQ=255)                             actCTAGTCATTATCTCACTCAGGCCGGGTTTACTTATATGCAGCC‑GGCTTTTTTTTCAAGAAATTATGGAGAAAAACGACAGGGAAATAGGTGAAATTCTCAATAAATGCGGTAACTAAGAGATTAGGATTGCGGAGAATAACAACCGCCGTACTCATCGCGTAATCTCCGGATATAAACCCATAACGGGCAATGCTAACAGGA‑TCACCATATGAAAAAGCTGAAATCTATCGTACTCGAACTTTCCCTg                                                                                                                                                                                                 >  2:109328/3‑251 (MQ=255)                              aTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTgg                                                                                                                                                                                                >  2:178695/1‑251 (MQ=255)                                    tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTgg                                                                                                                                                                                          >  1:411235/1‑251 (MQ=255)                                      aTTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTGTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                                                                                                                                                                                        <  2:212253/251‑1 (MQ=255)                                                 cAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgc                                                                                                                                                                             >  2:358045/1‑251 (MQ=255)                                                     ccGGGTGTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGATAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGAGTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAATATGCAATCTATCGTACTCGCACTTTCCCTCGTTCTGGCCGCTCCCATGgcagca                                                                                                                                                                         <  2:207439/251‑1 (MQ=255)                                                               ttttATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg                                                                                                                                                               >  1:448274/1‑251 (MQ=255)                                                                tttATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg                                                                                                                                                               >  1:329905/1‑250 (MQ=255)                                                                             gCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGt                                                                                                                                                  <  2:305462/251‑1 (MQ=255)                                                                             gCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGACAGCACAGGCTGCGGAAATTACGTTAGt                                                                                                                                                  >  1:329642/1‑251 (MQ=255)                                                                                     aTGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTc                                                                                                                                            >  1:143343/1‑249 (MQ=255)                                                                                            aaTTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAAtt                                                                                                                                   <  1:178695/251‑1 (MQ=255)                                                                                                 tGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTAc                                                                                                                                 >  1:550629/1‑248 (MQ=255)                                                                                                      aaaaaCGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGc                                                                                                                         <  1:59013/251‑1 (MQ=255)                                                                                                      aaaaaCGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGc                                                                                                                         <  2:512233/251‑1 (MQ=255)                                                                                                          acgaccgggAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGCTATCGACCCATAACGGGCCATGATAAAAGGAGTAACC‑TATGCAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCAATGGCAGCACGGGCTGCGGAAATTAAGTTAGTCCCGTCAGTAAAATTAAAGATAGGCGATc                                                                                                                     >  1:312192/7‑251 (MQ=255)                                                                                                             aCAGGGAAAAAGGAGACATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTg                                                                                                                  >  1:130876/1‑251 (MQ=255)                                                                                                                   aaaaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATc                                                                                                            >  2:179172/1‑251 (MQ=255)                                                                                                                     aaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGAGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATACCGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGt                                                                                                          >  1:89584/1‑251 (MQ=255)                                                                                                                      aaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTg                                                                                                         <  1:132884/251‑1 (MQ=255)                                                                                                                             aaTTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTAtt                                                                                                   >  2:181712/1‑250 (MQ=255)                                                                                                                              aTTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTa                                                                                                  >  1:219553/1‑250 (MQ=255)                                                                                                                                   cAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TAGGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTg                                                                                               <  1:363592/248‑1 (MQ=255)                                                                                                                                          tGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGt                                                                                     >  1:583388/1‑251 (MQ=255)                                                                                                                                                  cTTAGAGCTTAGGACTGCGGAGAATAACAACAGTCGTTCTCATAGCGTAATCTCCGGATATCGACCAATAACGGGCACTGATAAAAAGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCAATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGACAAATTACAGCACGGCGAACGTGATAATCGTGGCTCTTACTGGGATGGAGGCCACTGgcg                                                                             >  2:275291/1‑251 (MQ=255)                                                                                                                                                         aTTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATACTCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCAc                                                                      >  2:499542/1‑251 (MQ=255)                                                                                                                                                             ggATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCTGATATCGACCCATAACGGGCAATGATAAAATGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCTCACTTTCCCTTGTTCTTGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCTTCAGTAAAATTACAGATAGGCGATCGTg                                                                                                                  <  2:60704/203‑1 (MQ=255)                                                                                                                                                             ggATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGAACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTg                                                                                                                  >  1:60704/1‑203 (MQ=255)                                                                                                                                                                  gCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACGGGGATGGAGGTCACTGGCGCGACCACGGCtggtg                                                              <  1:165200/250‑1 (MQ=255)                                                                                                                                                                   cGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACACATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGACTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGAGCGTGATAATCGTGGCTATTATTGGGTTGGAGGTCACTGGCGCGACCACGACtggtgg                                                             >  1:478228/1‑250 (MQ=255)                                                                                                                                                                    ggAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGaa                                                           <  1:401410/251‑1 (MQ=255)                                                                                                                                                                                   tcgTTCTCATCGCGAAATCTCCGGATATAGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTAGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATAGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATg                                            >  2:286175/2‑251 (MQ=255)                                                                                                                                                                                     gTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGc                                          >  1:227347/1‑251 (MQ=255)                                                                                                                                                                                           aTCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATACAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGc                                     >  2:12607/1‑250 (MQ=255)                                                                                                                                                                                              gcgTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAAGCGATCGTGATAATCGTGGCTATTACTGGGTTGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATc                                 >  2:462938/3‑251 (MQ=255)                                                                                                                                                                                                   agcaccGGAGATCGACCCATAACGGTCAATGATAAAAGGAGTAACC‑TAGGAAAAAGATGCAATCTATCGTACTCGCCCTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTTCGGAAATTACGTTATTCCCGTCAGTAAAATTACAGATAGTCGCTCGTGATAATCGTGGCTATTACTGGGATGGAGGTCCCTGGCTCGGCCACTGCTAGTGGAAACAACATTATGAATGGCGAGGCAATCGCTgg                            <  2:257674/247‑1 (MQ=255)                                                                                                                                                                                                   aTCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTgg                            >  1:181074/1‑251 (MQ=255)                                                                                                                                                                                                         ggATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCAccc                       >  1:67416/1‑249 (MQ=255)                                                                                                                                                                                                                          gggCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAAGTGCGAGGCAATCGCTGGCACCCCCACGGACCGCCGCCAcc     >  2:147303/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaTGATAAAAGGACTAACC‑TATGAAAAAGATGCAATCTATCGTACTAGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGCCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGCTAGGAGAGGCAATCGCTGGCAACCACACGGACCGCCGCCAccgcc  >  2:572012/2‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      GCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCC  >  NC_000913/4993‑5463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑