Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,616,073	T→C	E331E (GAA→GAG)	yjjI ←	DUF3029 domain‑containing protein YjjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,616,073	0	T	C	100.0%	207.5 / NA	69	E331E (GAA→GAG)	yjjI	DUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/33);  total (36/33)

AGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGT  >  NC_000913/4615827‑4616316                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTcac                                                                                                                                                                                                                                                  >  1:86056/1‑251 (MQ=255)   cATGATGCGTGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGAGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTcacac                                                                                                                                                                                                                                                >  2:529396/1‑251 (MQ=255)         gCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACCGCa                                                                                                                                                                                                                                            >  2:12533/1‑249 (MQ=255)              acGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACCCCGCAAGTTAAc                                                                                                                                                                                                                                     >  2:11037/2‑251 (MQ=255)                gACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCg                                                                                                                                                                                                                                   >  1:239718/1‑251 (MQ=255)                               ggTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCCAGTTAAACGCTTCCGCCAGCCCAt                                                                                                                                                                                                                    >  1:99100/1‑251 (MQ=255)                                 tGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc                                                                                                                                                                                                                  >  2:21898/1‑251 (MQ=255)                                    tCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCACTCCCTTCTTTCTCACACCGCAAGGTAACCGCTTCCGCCAGCCCATACATg                                                                                                                                                                                                               >  1:126829/1‑251 (MQ=255)                                    ccGGACCTGGCTCATCGCCATACGGCACACCCGCGCCCGGCGTGGTTCCGAGAACGGAACTGTTCCCCGACTGTGCGTGTAACATGGCGCGTTTTGGCCAGCCATATTTCACGGTGGTAGTGGCGACAAACTCCGCCTGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCTTAGCGCGCGGCAATCCCTGCTTGCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCACACATg                                                                                                                                                                                                               <  2:370559/250‑1 (MQ=255)                                       gATCTGGCTCATCGCCCTAAGGCAAACGCGCCCCCGACGTGGTGCCGCTATCGGACCTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGAAAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCCCTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACCCAGCACGTTAACCGCTTCCGCCAGCCCATACATGccc                                                                                                                                                                                                            >  2:40819/1‑250 (MQ=255)                                           tGGCTCAACCCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGTACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCCGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTTTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCCCTTCCGCCAGCCCATACATGCCAAAc                                                                                                                                                                                                         >  2:37012/1‑250 (MQ=255)                                            ggCTCATCGCCATCCGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACa                                                                                                                                                                                                        <  2:494259/250‑1 (MQ=255)                                            ggCTCATCGCCAGACGGCAAACGCGCGCCCGGCGTGGTGCCGATACCGGAACTGATCCCCGACTGGGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCa                                                                                                                                                                                                            <  1:299460/246‑1 (MQ=255)                                             gCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACAtt                                                                                                                                                                                                      <  2:216995/251‑1 (MQ=255)                                                cATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGc                                                                                                                                                                                                   >  1:541024/1‑251 (MQ=255)                                                        tACGACAAACGCGCGCCCGGAGTGCTGCCGATATCGCAACTGATACCAGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTAACGGGGGCATTCGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTTCCGTAGCGTGCAGCAATCCCTTCTTTCTCACACAGCCAGTTAACCGCTTCCGCCAGACCATCAATGCCAAACATTGGccccact                                                                                                                                                                                             >  2:137411/1‑243 (MQ=255)                                                               aaCGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAggg                                                                                                                                                                                    <  1:211416/251‑1 (MQ=255)                                                                           gagtggagCCGATATCGGAACTGATCCCCGACTGTGCGTGTACCATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGctct                                                                                                                                                                        <  2:158547/249‑4 (MQ=255)                                                                               ggtgCCGATTTCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGTGTTAATCAGCCcttct                                                                                                                                                                     <  1:13787/250‑1 (MQ=255)                                                                                                 gATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATtct                                                                                                                                                 <  1:95484/251‑1 (MQ=255)                                                                                                     cccGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCaa                                                                                                                                              >  1:454761/1‑250 (MQ=255)                                                                                                      ccGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCaa                                                                                                                                              >  1:344903/1‑249 (MQ=255)                                                                                                           tgtgCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAgtg                                                                                                                                       >  1:398229/1‑251 (MQ=255)                                                                                                           tgtgCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTAGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAgtg                                                                                                                                       >  1:30894/1‑251 (MQ=255)                                                                                                            gtgCGTGTAACATGGCGCTTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAgtg                                                                                                                                       <  2:174233/250‑1 (MQ=255)                                                                                                               cGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGAt                                                                                                                                   <  1:540359/251‑1 (MQ=255)                                                                                                               cGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGAt                                                                                                                                   >  1:126182/1‑251 (MQ=255)                                                                                                                         ggCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGAt                                                                                                                            >  1:597784/1‑248 (MQ=255)                                                                                                                           cgcgTTTTTGCCAGCCATATGTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGTGCGCTGATGCTATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCTCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCGTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTGTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAgg                                                                                                                       <  1:93954/251‑1 (MQ=255)                                                                                                                            gcgTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGa                                                                                                                      <  1:140432/251‑1 (MQ=255)                                                                                                                             cgTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGACAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTGCTCACACAGTAAGTTAATCGCTTCCGCCAGCCCATACATGCCAAACATTGGCTCAAAACGTTCAGGGTTAATCAGCCCTGCTTTC‑ACCAGGAAGCTAGTCTCAAAGAAGTGTGATTGTTGATAGAGGaa                                                                                                                     <  1:557932/251‑1 (MQ=255)                                                                                                                               tttttGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCCGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGAGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAAtt                                                                                                                   >  1:68775/1‑251 (MQ=255)                                                                                                                                          caTATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCgcgcc                                                                                                        >  2:138409/1‑250 (MQ=255)                                                                                                                                          caTATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCa                                                                                                        <  1:37012/251‑1 (MQ=255)                                                                                                                                             atTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATc                                                                                                      <  1:410515/250‑1 (MQ=255)                                                                                                                                                 cACGGGGGTATTGGCGACAAACTCCGCAAGTTGCGCGATGATGCGATAACCGACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGACACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACTAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACAACGCGCATCgttg                                                                                                  >  1:449134/1‑247 (MQ=255)                                                                                                                                                    gggggTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATgg                                                                                              <  1:12533/251‑1 (MQ=255)                                                                                                                                                     ggggTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGAGTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGc                                                                                             <  2:272378/251‑1 (MQ=255)                                                                                                                                                      gggTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCg                                                                                            <  1:15391/251‑1 (MQ=255)                                                                                                                                                          agtGGCGACAAACGCCGGCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAAGCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACCCCGCGCATCGATGATGGCGATc                                                                                         <  2:590136/248‑1 (MQ=255)                                                                                                                                                                    aaCTCCGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCCGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATctgctgcttgc                                                                               >  2:6771/1‑247 (MQ=255)                                                                                                                                                                         cGCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCagtagt                                                                         <  1:241487/251‑1 (MQ=255)                                                                                                                                                                          gCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCCTACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑CCCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATCGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTg                                                                        >  2:412251/1‑251 (MQ=255)                                                                                                                                                                          gCCAGTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGCTAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTg                                                                        >  2:46996/1‑251 (MQ=255)                                                                                                                                                                            cAGTTGCGCGCTGATGCGATAACCCACTGCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCg                                                                      <  1:216856/251‑1 (MQ=255)                                                                                                                                                                              gTTGCGCGCTGATGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGt                                                                    <  2:239718/251‑1 (MQ=255)                                                                                                                                                                                tgcgcgacgATGCGATAACACACCTCATTTGCGGCGGCCTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTAACACCGCAAGTTAACCGCTTCCGACCGCCAATACACGCCCAACATTGGCACAAAACGTTCAGGGCTAATCAGCCCTTCTTTCCACC‑GGCAGCTATTCTCCAAGCCGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGt                                                                    >  2:255430/9‑249 (MQ=255)                                                                                                                                                                                        gATGCGATAACCCACTTCATTTGCGGCGGCTTATTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGTTAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGcgcg                                                          >  1:457147/1‑251 (MQ=255)                                                                                                                                                                                         aTGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGt                                                         >  1:267711/1‑251 (MQ=255)                                                                                                                                                                                          tGCGATAACCCACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGt                                                         <  1:26869/250‑1 (MQ=255)                                                                                                                                                                                           gCGATAACCCACTGCATTTGCGGCGGCTTCTTTACCGTAGCTCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCGTCTTTC‑GCCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGGAGAGTGCGCGTa                                                        <  2:374946/250‑1 (MQ=255)                                                                                                                                                                                                    caCTTCATTAGCGGCGTCTTCTTTACCGTAACGCGCAGCAATCCCTTCTTTCTCTCACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGATCAGGGCTCATCAGCCCGTCTTTC‑ACCCGGGAGCTCTTCTCGAAGAAGTGTGATTGTTGTTTGAGGATTTCACACTGCGCATCCATGATGGCGATCTGCTGCTGGTAGTGGTGCGGTAGAGTGCGCGTTAAGAAGTCAt                                              >  2:5456/2‑251 (MQ=255)                                                                                                                                                                                                     aCTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCGCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGGGCGCGTAAAGAAGTCATc                                             <  2:547935/251‑1 (MQ=255)                                                                                                                                                                                                      cTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATcc                                            >  2:218501/1‑251 (MQ=255)                                                                                                                                                                                                         cATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGc                                         <  2:126829/251‑1 (MQ=255)                                                                                                                                                                                                              gctgcggcTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACTCCGCGCATCGATGATGGCGAGCTGCTGCTGGCAGTAGTGCGGTAGAGGGCGCGTAAAGAAGTGATCCAGCGAtt                                     <  2:86056/247‑1 (MQ=255)                                                                                                                                                                                                                 gcggcTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCt                                 >  1:268317/1‑251 (MQ=255)                                                                                                                                                                                                                 gcggcTTCTTTACCGTAGCGCGCGGCAAGCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAAGTCACACCGCGCAGCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCt                                 <  1:404555/251‑1 (MQ=255)                                                                                                                                                                                                                       tcttTACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑TCCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCAGCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCt                            <  1:42039/250‑1 (MQ=255)                                                                                                                                                                                                                          ttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCgg                         <  1:224414/250‑1 (MQ=255)                                                                                                                                                                                                                          ttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCg                          <  2:117079/249‑1 (MQ=255)                                                                                                                                                                                                                           tACCGTAGCGCGCGGCCATCCCTTCTTTCTCACACAGCAAGTTAAACGCTTCCGCCAGCCCATACATGCCAAACCTTGGCACCACACGTTCAGGGTTCATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGCGGAATTCATACAGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTCGCGTGCGCGTACAGAAGTCATCCAGCGATTCGCTGCGCTCGGcc                       >  2:292430/1‑250 (MQ=255)                                                                                                                                                                                                                              cGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTAAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATg                    >  2:122514/1‑251 (MQ=255)                                                                                                                                                                                                                               gTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCCAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGAGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGCCATCCAGCGCTTCGCTGCGCTCGGCAATgg                   >  1:539940/1‑251 (MQ=255)                                                                                                                                                                                                                               gTAGCGCGCGGCAATCCCTTCTTTCTCACAAAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATgg                   >  1:198424/1‑251 (MQ=255)                                                                                                                                                                                                                                     cgcgGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCa             <  2:398229/251‑1 (MQ=255)                                                                                                                                                                                                                                         gCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTGCAGGtt         <  2:477542/251‑1 (MQ=255)                                                                                                                                                                                                                                          cAATCCCTTCTTTCTCACAAAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTGCAGGtt         >  2:391957/1‑250 (MQ=255)                                                                                                                                                                                                                                                cttcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAACCATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGCGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGt  >  1:416176/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTTTTGCCAGCCATATTTCACGGGGGTATTGGCGACAAACTCCGCCAGTTGCGCGCTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAAACGTTCAGGGTTAATCAGCCCTTCTTTC‑ACCAGGAAGCTATTCTCAAAGAAGTGTGATTGTTGATAGAGGAATTCACACCGCGCATCGATGATGGCGATCTGCTGCTGGCAGTAGTGCGGTAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGT  >  NC_000913/4615827‑4616316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑