Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,099	T→C	intergenic (+79/‑135)	thrC → / → yaaX	threonine synthase/DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,099	0	T	C	100.0%	170.3 / NA	52	intergenic (+79/‑135)	thrC/yaaX	threonine synthase/DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/29);  total (23/29)

CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCG‑TGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGC  >  NC_000913/4855‑5344                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gggccccgcgctcccgggaaaaTTTAAAG‑GAGGGGTGGAAGCGTTTCCCGGGGAACCGTTGGATCGGCCAAAAGAGCTGGAAGAACGGTCTGATTTA‑CCCTTTCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAggg                                                                                                                                                                                                                                                 <  2:475593/232‑1 (MQ=255)     aCCGCGCATCCGGCGAACTTTAAAGAGAGCG‑TGGAAGCGATTCTCGGGGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGaaa                                                                                                                                                                                                                                              >  2:529777/1‑250 (MQ=255)          cctgccGGCGAAATTAAAAGAGGGTG‑GGGAAGCTGTTCCCGGTAAAACGTTGTACCTGCCAAAAGGGCGTGCAGAACTTGGGGATTAT‑CCCTTTTTTTCACAAAATCGTCCCGCCGAGTTTGCTGCGTTGCGTAAATTGATGATGAATTATCATTAAAATCTATTCATTAGCTCAATCAGGTCGGGTTTGCTTTTATGCA‑TCCGGCTTTTTTATGAAGAAATTATGAAGAAAAACGACATGGAAAgaggag                                                                                                                                                                                                                                        <  2:605638/247‑1 (MQ=255)            aTCCGGCGAAATTTAAAGAGAGCG‑TGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGgaga                                                                                                                                                                                                                                       >  2:132884/1‑250 (MQ=255)                        ttAAAGAGAGCG‑TGGAAGCGATTCTCGGTGAAACTTTGGATCTGCCAAAAGAGCTGGCAGAACTTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATaa                                                                                                                                                                                                                           <  1:65467/250‑1 (MQ=255)                              agaagg‑TGGAGGCAATTCTCTGTGAACCGTTGGATCTGCAAAAAGAGCTGGCAAAAAGTGGTG‑TTTATCCCGTGTTTTCACAAAAACTGACCGCCGATGTTTCTGCGTTGCGTAAATTGATGATGAATCATTAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCA‑GGATTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCGCAAGAAATGCGGt                                                                                                                                                                                                                    <  2:198663/246‑1 (MQ=255)                                     tGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAAt                                                                                                                                                                                                                         >  2:373966/1‑240 (MQ=255)                                     tGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAAt                                                                                                                                                                                                                         <  1:373966/240‑1 (MQ=255)                                     tGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTATCACTAAATGCGGTAACtt                                                                                                                                                                                                               >  2:402884/1‑250 (MQ=255)                                         aGCGATCCTCGGGGAAACGTTGTATCGGACAAAAGAGCTGGCAGAACGTGCTGATTGC‑CCCTTGCTTACACATACTCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAACTTCTCAATAAATGCCGTAACTTAgaga                                                                                                                                                                                                          <  2:217531/251‑1 (MQ=255)                                             aTTCTCGGTGAAACGTTGGATCGGCAAAAAGAGCTGGCAGAACGGGCTGATTTA‑CCCTTGCTTTCACATAACTTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAg                                                                                                                                                                                                      <  1:342785/251‑1 (MQ=255)                                                          cGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCAAATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCGGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGCAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAAAAt                                                                                                                                                                                         >  1:16018/1‑251 (MQ=255)                                                                    gCCAAAAGGGCGGGCCGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaac                                                                                                                                                                                   <  2:521626/247‑1 (MQ=255)                                                                           gagCTGGCAGAACGTGTTGATTTT‑CCCTTTTTTTCTCAATATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTATATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAAGGCGGTAACTTAGAGAGTAGGAGTGCGGTGAATAACAACCGTCGTTCTCa                                                                                                                                                                        <  2:315340/251‑1 (MQ=255)                                                                                        gTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTcc                                                                                                                                                           >  2:525338/1‑251 (MQ=255)                                                                                              aTTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGatat                                                                                                                                                     >  2:473191/1‑251 (MQ=255)                                                                                                  a‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGAc                                                                                                                                                 <  1:409543/251‑1 (MQ=255)                                                                                                     ccTTGTTTCCACAAAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCAGCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGTAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGAccc                                                                                                                                               <  2:623051/251‑1 (MQ=255)                                                                                                        tgtttttaaaaagtgtggccgccgATTTTGCTGCGTTGCGTAAATTGATGATGAAGCATCAGTAAAATCTATTCATTATCACAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAGCGACAGGGAAAAAGGAGAAAGTCTCAATAAATGCGGTAACTTAGGGAGTTGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCAt                                                                                                                                             <  2:236404/232‑1 (MQ=255)                                                                                                                  aaaTCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATGAGGAGTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATGACGGGCAATg                                                                                                                                  <  2:595073/250‑1 (MQ=255)                                                                                                                    aTCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                                                                                >  1:537329/1‑251 (MQ=255)                                                                                                                        gCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaaa                                                                                                                            <  1:558458/251‑1 (MQ=255)                                                                                                                          ccgccgATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTAATCATTATCTCAATCAGGCCGGGTGTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                                                          <  1:229716/251‑1 (MQ=255)                                                                                                                             ccgATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAACCGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                                                       <  1:473191/251‑1 (MQ=255)                                                                                                                               gATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa                                                                                                                      >  1:581382/1‑250 (MQ=255)                                                                                                                                aTTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaac                                                                                                                                                                                   >  1:284484/1‑188 (MQ=255)                                                                                                                                aTTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAAGaacaac                                                                                                                                                                                   <  2:284484/188‑1 (MQ=255)                                                                                                                                  tttGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑t                                                                                                                 >  1:487551/1‑251 (MQ=255)                                                                                                                                    tgctgcGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑tat                                                                                                               >  1:329579/1‑249 (MQ=255)                                                                                                                                             gcgtAAATTGAGGAGGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAATAACGACAGGGAAAAAGGAGAAAGTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTGCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGAt                                                                                                      <  1:36974/251‑1 (MQ=255)                                                                                                                                               gtAAATTGATGATGAATAATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGc                                                                                                    >  2:87016/1‑251 (MQ=255)                                                                                                                                                 aaaTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCa                                                                                                   >  1:346485/1‑250 (MQ=255)                                                                                                                                                      gatgatgaATCATCAGTAAAAACTATTCATTATCTCAATCAGGCCGGGTTTGCTTGTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCAACGCGTAATCTCCGGATAACGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATcga                                                                                              <  2:525317/250‑3 (MQ=255)                                                                                                                                                         gatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCg                                                                                           <  1:400548/250‑1 (MQ=255)                                                                                                                                                            gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTAc                                                                                        <  1:164638/250‑1 (MQ=255)                                                                                                                                                                catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCa                                                                                   <  2:166611/251‑1 (MQ=255)                                                                                                                                                                   caGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACtt                                                                                <  2:2479/251‑1 (MQ=255)                                                                                                                                                                         actCTAGTCATTATCTCACTCAGGCCGGGTTTACTTATATGCAGCC‑GGCTTTTTTTTCAAGAAATTATGGAGAAAAACGACAGGGAAATAGGTGAAATTCTCAATAAATGCGGTAACTAAGAGATTAGGATTGCGGAGAATAACAACCGCCGTACTCATCGCGTAATCTCCGGATATAAACCCATAACGGGCAATGCTAACAGGA‑TCACCATATGAAAAAGCTGAAATCTATCGTACTCGAACTTTCCCTg                                                                          >  2:109328/3‑251 (MQ=255)                                                                                                                                                                          aTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTgg                                                                         >  2:178695/1‑251 (MQ=255)                                                                                                                                                                                tCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTgg                                                                   >  1:411235/1‑251 (MQ=255)                                                                                                                                                                                  aTTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTGTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                                                                 <  2:212253/251‑1 (MQ=255)                                                                                                                                                                                             cAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgc                                                      >  2:358045/1‑251 (MQ=255)                                                                                                                                                                                                 ccGGGTGTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGATAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGAGTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAATATGCAATCTATCGTACTCGCACTTTCCCTCGTTCTGGCCGCTCCCATGgcagca                                                  <  2:207439/251‑1 (MQ=255)                                                                                                                                                                                                           ttttATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg                                        >  1:448274/1‑251 (MQ=255)                                                                                                                                                                                                            tttATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg                                        >  1:329905/1‑250 (MQ=255)                                                                                                                                                                                                                         gCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGt                           <  2:305462/251‑1 (MQ=255)                                                                                                                                                                                                                         gCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGACAGCACAGGCTGCGGAAATTACGTTAGt                           >  1:329642/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTc                     >  1:143343/1‑249 (MQ=255)                                                                                                                                                                                                                                        aaTTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAAtt            <  1:178695/251‑1 (MQ=255)                                                                                                                                                                                                                                             tGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTAc          >  1:550629/1‑248 (MQ=255)                                                                                                                                                                                                                                                  aaaaaCGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGc  <  2:512233/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaaaCGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGc  <  1:59013/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCG‑TGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTA‑CCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACC‑TGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGC  >  NC_000913/4855‑5344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑