Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,563,567	T→C	I13V (ATT→GTT)	yjiK ←	PF06977 family protein YjiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,563,567	0	T	C	100.0%	165.2 / NA	51	I13V (ATT→GTT)	yjiK	PF06977 family protein YjiK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CGGATAAAATCACCATTGGTGGTCATTTCAACAATGGCGGCAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCG  >  NC_000913/4563326‑4563812                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cGGATAAAATCACCATTGGTGGTCATTTCAACAATGGCGGCAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCg                                                                                                                                                                                                                                              >  1:428117/1‑251 (MQ=255)   gATAAAATCACCATTGGTGGGCATTTCAACAATGGCGGAAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTAGTTATGCCTGCAATTTCTTTGCCGTCAATGGGGGCGTGGTAATTCTGAAATGATGCTGCCTGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGAACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCgct                                                                                                                                                                                                                                            >  1:2644/1‑249 (MQ=255)      aaaaTCACCATTGGTGGTCATTTCAACAATGGCGGCAGGTTTGTTGATAGCGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGAGTTTAc                                                                                                                                                                                                                                         >  1:177224/1‑251 (MQ=255)                            tCAACAATGGCGGCAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCa                                                                                                                                                                                                                    <  2:591596/250‑1 (MQ=255)                                 aaTGGCGGCAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGGCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTa                                                                                                                                                                                                               >  1:280672/1‑250 (MQ=255)                                           ggTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAg                                                                                                                                                                                                    <  1:89925/251‑1 (MQ=255)                                              ttgttgATAGTGCTAAAAAGAGTATTGCTTTGGTCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGAGTGCTTGTACGGGCGCAAGATTGCACGAAGAAAGTACGCACTTCCACAATGGCAAAAAGAATCACAATAACGGAGATTCGTGTACTTAACGAGATACTTTTTGTTACgggtagtgcgctaagtc                                                                                                                                                                                                  <  2:91043/250‑16 (MQ=255)                                                gttgATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCaaa                                                                                                                                                                                               <  1:175640/251‑1 (MQ=255)                                                   gATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCa                                                                                                                                                                                            <  2:432678/251‑1 (MQ=255)                                                    aTAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCaa                                                                                                                                                                                           <  1:396338/251‑1 (MQ=255)                                                      aGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCTAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACTCACTGCCACAATGGCAAAAAGAATCACAATAACGTAGATGCTTTTACTTAACGAAATACTTTCTGTCACGGTAATCCCTTTAAGGCTCATCAAt                                                                                                                                                                                          <  2:92193/250‑1 (MQ=255)                                                           tAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGat                                                                                                                                                                                     >  2:365619/1‑250 (MQ=255)                                                            aaaaaGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGatat                                                                                                                                                                                   <  1:317080/251‑1 (MQ=255)                                                            aaaaaGAGTATTGCTTTGTGCCGAACAGGTCAGCGCAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATACCTTTAAGGCAAATCAATAAGatat                                                                                                                                                                                   >  2:459610/1‑251 (MQ=255)                                                                           ttGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACg                                                                                                                                                                      <  1:144109/249‑1 (MQ=255)                                                                              tgCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCg                                                                                                                                                                 >  1:359714/1‑251 (MQ=255)                                                                                 cGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGAcc                                                                                                                                                              >  1:11003/1‑251 (MQ=255)                                                                                    ccAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTa                                                                                                                                                           <  1:184321/251‑1 (MQ=255)                                                                                              aaaGAGATATTATTCGTTATGCCTGCAATTTCTTGGCCGTCAATGGTGGCGTGGTAATTCTGAAAGGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCtt                                                                                                                                                 <  2:599245/250‑1 (MQ=255)                                                                                                      attattCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGga                                                                                                                                         <  2:446139/251‑1 (MQ=255)                                                                                                         attCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGgaga                                                                                                                                       >  1:33984/1‑250 (MQ=255)                                                                                                         attCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGgaga                                                                                                                                       >  1:305980/1‑250 (MQ=255)                                                                                                          ttCGTTATGCCTGCAATTTATTTGCCGTCAATCGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCCAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGaa                                                                                                                                      >  2:392386/1‑250 (MQ=255)                                                                                                                       cAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATTCTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGc                                                                                                                        <  1:346060/251‑1 (MQ=255)                                                                                                                               ttGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCa                                                                                                                  <  2:626661/249‑1 (MQ=255)                                                                                                                                  ccGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGcc                                                                                                             <  1:489694/251‑1 (MQ=255)                                                                                                                                  ccGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAAGCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCTGAGAAACCCCGTAATGTGCAATGCAGCGcc                                                                                                             <  2:591929/251‑1 (MQ=255)                                                                                                                                        aTGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTGTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGacac                                                                                                       <  1:507043/251‑1 (MQ=255)                                                                                                                                          ggtggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACAcc                                                                                                      <  2:76266/250‑1 (MQ=255)                                                                                                                                          ggtggCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCAAAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACAcc                                                                                                      >  2:332957/1‑250 (MQ=255)                                                                                                                                                    tAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACACTGGCAAAAAGAATCACAATAACGGAGATGCGTTTCCTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGCAGTATACGTAGCGACCTTAATGAATACTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCctgctgtc                                                                                           >  1:72151/1‑249 (MQ=255)                                                                                                                                                     aaTTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCg                                                                                                                                <  2:434761/213‑1 (MQ=255)                                                                                                                                                     aaTTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCg                                                                                                                                >  1:434761/1‑213 (MQ=255)                                                                                                                                                       ttCTGAACTGAAGCTGCATGATTGCTTTTACGGGCGCAAGATTGCCCGAAGACAGTACACACTGCCACCATGGAAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATCCTTTTTGTCACGGTACTCCCTTTAAGCCAACTCAATAAGATATCGAGACGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTCATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAAt                                                                                        >  1:313121/1‑251 (MQ=255)                                                                                                                                                                gatgCTGCATGATTGCTTTTACGGGCGCACGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACt                                                                               >  1:221373/1‑251 (MQ=255)                                                                                                                                                                        aTGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGCTGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGc                                                                       >  1:154275/1‑251 (MQ=255)                                                                                                                                                                             tGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAgg                                                                  >  2:572543/1‑251 (MQ=255)                                                                                                                                                                                      gggCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGa                                                                                 <  2:127107/227‑1 (MQ=255)                                                                                                                                                                                      gggCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCa                                                         <  1:365619/251‑1 (MQ=255)                                                                                                                                                                                      gggCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGCAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGa                                                                                 >  1:127107/1‑227 (MQ=255)                                                                                                                                                                                                  gCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTc                                             >  1:272432/1‑251 (MQ=255)                                                                                                                                                                                                             gTACACACTGCCACAATGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGAc                                  >  1:451795/1‑251 (MQ=255)                                                                                                                                                                                                                      gCCACAATGGCAAAACGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATACCTTTAAGGCAAATAAATAAGATATCGAGAAGTATACGAAGCGACCTTAATGAATCCTTAACGCGGCGAAACCCCGTAATGTGCAATGCAGCGCCAGACACAATCCTGCTGACAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCACCGGAGGGACAGGCCAGcc                         >  2:542478/1‑251 (MQ=255)                                                                                                                                                                                                                      gCCACAATGGCAAAAAGACTCACAATAACGGAGATGCGTTTACTTAACGCAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCAAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGcc                         >  2:587342/1‑251 (MQ=255)                                                                                                                                                                                                                      gCCACAATGACAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACACATTGTATTTCCTCGCCGGGTGGACAGGTCAGcc                         >  2:508191/1‑251 (MQ=255)                                                                                                                                                                                                                           aaTGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATCCTTTTTGTCACGGTAATCCCTTTAAGGCAACTCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCATTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATg                    >  1:537815/1‑251 (MQ=255)                                                                                                                                                                                                                           aaTGGCAAAAAGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATg                    >  1:124244/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaaaaGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCCCATCACTAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGACAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATacc              >  2:218091/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaaGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAAccc            <  1:152000/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCAGTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCg           <  2:519664/251‑1 (MQ=255)                                                                                                                                                                                                                                     aGAATCACAATAACGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGc          <  1:423564/251‑1 (MQ=255)                                                                                                                                                                                                                                             aataacGGAGATACGTATACTTAACGAACTACTTTTTGTCACGGTAATCTCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTCATGAATCCTTAACGCGGCGAATCCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGAAATAAATTGACTCAGTCAGCCCAGGGATCCCCCATTGTATTTACTCGCCGGGGGGACAGGTCCGCCAACTGCATCACACGCAGccgccg  >  1:614997/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CGGATAAAATCACCATTGGTGGTCATTTCAACAATGGCGGCAGGTTTGTTGATAGTGCTAAAAAGAGTATTGCTTTGTGCCGACCAGGTCAGCGAAGAGATATTATTCGTTATGCCTGCAATTTCTTTGCCGTCAATGGTGGCGTGGTAATTCTGAAATGATGCTGCATGATTGCTTTTACGGGCGCAAGATTGCACGAAGAAAGTACACACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCG  >  NC_000913/4563326‑4563812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑