Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,822	T→C	D363D (GAT→GAC)	thrC →	threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,822	0	T	C	100.0%	194.9 / NA	62	D363D (GAT→GAC)	thrC	threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/38);  total (24/38)

CCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTT  >  NC_000913/4579‑5058                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             ccTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCTCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTg                                                                                                                                                                                                                                        <  1:412990/250‑1 (MQ=255) ccTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTg                                                                                                                                                                                                                                        >  1:198663/1‑250 (MQ=255)    gCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATc                                                                                                                                                                                                                                    >  2:180692/1‑251 (MQ=255)      acgacgGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATcc                                                                                                                                                                                                                                   >  1:623051/1‑250 (MQ=255)        gagggtaagtggccccccAAAGCGACTCAGGCGACGTTATCCAACGGGATGGACGTGAGTCAGCCGAACAACTGGGCGCGTGTGGAAGAGTTGTTCCGCCGCAAATTCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCTCGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAgg                                                                                                                                                                                                                                <  2:200014/236‑1 (MQ=255)           ggTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGAATCGTGCGCTGCGTGACCAGTTGCATCCAGGCg                                                                                                                                                                                                                              >  2:402899/1‑250 (MQ=255)                          aaaGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTccc                                                                                                                                                                                                              >  2:312827/1‑250 (MQ=255)                          aaaGCGACTCAGGAGACGTTATCCAACGCGATGGACGTGAGTAAGCCGAAAAACTGGCCGCGTGTGGAAGAGTTGTTCAGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACTACGCAAAAGACAATGAGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCAGTAGCGTATCGTGCGCTGAGCGACCAGTGCAATCCAGGCGAATATGGATTGttacc                                                                                                                                                                                                              >  2:146517/1‑248 (MQ=255)                            aGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCg                                                                                                                                                                                                            <  1:222498/251‑1 (MQ=255)                               gACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGc                                                                                                                                                                                                          >  1:502576/1‑250 (MQ=255)                                ccTCAGGCGACTTTATCCAACGCGATGGAGGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTTAATCCAGGCGAATATGGCTTGTTCCTCGGCAc                                                                                                                                                                                                        <  2:502626/250‑1 (MQ=255)                                       cGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCAt                                                                                                                                                                                                 <  1:396826/251‑1 (MQ=255)                                         aCGTTATCCACCGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATcc                                                                                                                                                                                               <  1:335938/251‑1 (MQ=255)                                         aCGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCCTcc                                                                                                                                                                                               >  2:480416/1‑251 (MQ=255)                                            ttATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGACGAGTTGTTCCGCCGCAAACTCTGGCAACTGCAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGGAGCGTATCGTGAGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGc                                                                                                                                                                                            >  2:473673/1‑251 (MQ=255)                                             tATCCAACTCGGTGGACGTTAGTCAGCCGAACACCGGGCCGCGTGTGGAAGAGTTGTGCCGCCGCAAAATCTGGCAACGTAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAAGCCAGGCGTATATGGCTTGTTCCTCGTCACCGCGCAGCCGGc                                                                                                                                                                                            <  2:576247/250‑1 (MQ=255)                                              acccAACGCGATGGACGTGAGTCGGCCGAACAACGGGCCGCGTGTGGAAGATTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCg                                                                                                                                                                                           <  1:443692/248‑1 (MQ=255)                                                    cgagatggcgttgattCAGACGAACAACGGGCCGCGTGTGGAAGAGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGGTGAATCCAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCCGGCGAAATTTa                                                                                                                                                                                    <  2:57304/236‑1 (MQ=255)                                                          ggACGTGGGTCAGCCGAACAACTGGCCGCGTGGGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga                                                                                                                                                                              <  2:338095/251‑1 (MQ=255)                                                             cGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCTGCACCGCGCATCCGGCGAAATTTAAAGAGAGCg                                                                                                                                                                           <  1:195817/251‑1 (MQ=255)                                                              gTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGt                                                                                                                                                                          >  2:558458/1‑251 (MQ=255)                                                                       ccGAACAACTGGCCGCGTGTGGAAGAGTGGTTCCGCAGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGGGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGAt                                                                                                                                                                 <  1:118417/251‑1 (MQ=255)                                                                              cagggccgggggtgGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGt                                                                                                                                                          <  2:2605/240‑1 (MQ=255)                                                                                 ggCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAAGTTAAAGAGAGCGTGGAAGCGATTCTCGGTGaa                                                                                                                                                       <  1:521993/251‑1 (MQ=255)                                                                                   cggcgggtgGAAGGGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAAc                                                                                                                                                     <  2:184268/245‑1 (MQ=255)                                                                                    agagtgtGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGACGCGATTCTCGGTGAAACg                                                                                                                                                    >  2:342785/4‑251 (MQ=255)                                                                                     gcgTGTGGAAGAGTGGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGGGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGt                                                                                                                                                   <  1:319368/251‑1 (MQ=255)                                                                                       gtgtGGAAGAGTTGCTCCGCAGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAACCCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGATTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGGGTGGCAGCGCTTCTCGGTGAAACGTTg                                                                                                                                                 >  2:382018/1‑251 (MQ=255)                                                                                         gtgGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTgg                                                                                                                                                <  1:357705/250‑1 (MQ=255)                                                                                             aaGAGTTGTTCAGCCGCAAAATCTGGCACCTGACAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGAAACAGACAATGAGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGAATTGTTCCTCGGGACCGCGCATCCGGCGCAATTTAAAGAGAGCGTGGAAGAGCTTCTCGGTGAAACGTGGGATCTg                                                                                                                                           >  2:49153/1‑251 (MQ=255)                                                                                               gagTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGcc                                                                                                                                         >  1:305462/1‑251 (MQ=255)                                                                                                    gttCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCaaaa                                                                                                                                     <  1:146207/250‑1 (MQ=255)                                                                                                    gttCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAag                                                                                                                                    <  1:133005/251‑1 (MQ=255)                                                                                                          cggcaaaatcgggtatgtGAAAGAGCTGGGGTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTGAAAAGAACTGGTCTACACCCCGGAGCCGCACGCTGCCGTAGCGAATGGTGCGGTGAGTGACCAGTTGAATTCAGGCGAATATGTTTGGTGCCTCTGCACCGCGTATCCGGCGAAATGTAAAGAGAGCGGGGAAGCGATTCTCGTTGAAACGTAGGAGCTTCCAAAACAGCTgg                                                                                                                              <  2:303860/234‑1 (MQ=255)                                                                                                             cAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCGCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAg                                                                                                                           <  1:180692/251‑1 (MQ=255)                                                                                                              aaaaTCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGCAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGc                                                                                                                             >  2:400548/1‑248 (MQ=255)                                                                                                                aaTCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAAc                                                                                                                        <  1:382018/251‑1 (MQ=255)                                                                                                                  tCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTg                                                                                                                                                                                                                                        <  1:200461/137‑1 (MQ=255)                                                                                                                  tCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTg                                                                                                                                                                                                                                        >  2:200461/1‑137 (MQ=255)                                                                                                                   cTGGCAACTGACAGAGCTGGGTTATGCACCCGTGGATGATAAAACCCCGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACACTGCCGTAGCGTATCGTGCGCTGAGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGCACCGATTCTCGGGGAAACGTTGGATCTGCCAAAAGCGCGGGCAGAACGTg                                                                                                                     >  2:229716/1‑251 (MQ=255)                                                                                                                   cTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGAATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGCAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTg                                                                                                                     >  1:400293/1‑251 (MQ=255)                                                                                                                    tGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGCGCGTGGAAGCGATTCTCGGTGAAAAGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGc                                                                                                                    >  1:212253/1‑251 (MQ=255)                                                                                                                        aaCTGAAAGAGCTGGGTTATGCAGCCGTTGATGATGAAACCACGTAACAGACAATGCGTGAGTTAAAAGAACTGGTCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGCTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGAt                                                                                                                <  1:16452/251‑1 (MQ=255)                                                                                                                         aCTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACCCCTCGGAGCTGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGTCGTATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGACTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGAtt                                                                                                               <  1:583280/251‑1 (MQ=255)                                                                                                                            gaaagttcggggTTGTGCAGCCGGGGAGGATGAAACCTCGCAAAATACAAGGCGTGAGGTAAAAGAACTGGGCGACTCCTCGGAGTCGCACGCTGCCGGAGCGGATCGTGCGCTGCGTGACCAGGTGAATCAAGGTGAAGAGGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAAGTTAAAGAGAGCGGGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCTGAACGTGCTGAtttgc                                                                                                            <  2:7306/242‑3 (MQ=255)                                                                                                                            gAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGAttt                                                                                                              <  2:486002/249‑1 (MQ=255)                                                                                                                             aaaGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTAcc                                                                                                           <  1:431329/251‑1 (MQ=255)                                                                                                                               agagCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGGGCTGATTTACCCt                                                                                                         >  2:164638/1‑251 (MQ=255)                                                                                                                                 agCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTg                                                                                                       <  2:65737/251‑1 (MQ=255)                                                                                                                                    tGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCtt                                                                                                    >  1:521626/1‑251 (MQ=255)                                                                                                                                            gccgCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACCCCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGTATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACAtt                                                                                             <  2:364483/247‑2 (MQ=255)                                                                                                                                             cAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAAt                                                                                           <  1:174817/251‑1 (MQ=255)                                                                                                                                                          ggaacccccGCAACAGACAAGGCGTGAGTGAAAAGAACGGGGCTCCACCTCGGAGCCGCACGCTGCCGTAGCGTAGCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGACCCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTGGCTTTCGCATAATCTGCCCGCCGAtt                                                                              <  2:231728/243‑1 (MQ=255)                                                                                                                                                            aaaCCACGCAACAGACAATGCGTGAGTTAAAAGACCTGGGCTACCCCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGAtttt                                                                            <  1:402899/251‑1 (MQ=255)                                                                                                                                                                                cGTGAGTTAAAAGAACGGGGCTACCCCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATtgat                                                        <  1:312827/251‑1 (MQ=255)                                                                                                                                                                                       tAAAAGAACTGTGCTACACATCGGAGCCGCACGCTGCCGTAGCGAATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAACTTTAAATAGAGCGTGGAAGCGATTCTCGGTGAAACGGTGGGTCTTCCAAAAAAGCTGGCAGCACGTGCTGATTTACCCTTTCTTTCACATAATCTGCCCGCCGATTTTGCTACGTTTCGTAAATTGATGAAGAat                                                 >  2:55675/1‑251 (MQ=255)                                                                                                                                                                                           aGAACTGGGCTACACCTCGGAGCCGCACGCTGCCGTAGCGTATCGTGCGCGGCGTGACCAGTTGAATCCAGGGGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGGGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAatcatc                                             <  1:480416/251‑1 (MQ=255)                                                                                                                                                                                                        ccctCGGTGCCGCCCGCTGCCGTAGCTTAGCTTGCTCTGCGTGCCCAGTTGAGTCCAGGCGAATATGGCTTGTTCCTCGGCCCCGCGCATCCGGAAAAATTTAAAGAGAGCGTGGAAGCGATTCTCGTTGAAACTTTGGATCTGCCAAAAGAGCTGGCAGACCGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTAtt                                <  2:310166/248‑1 (MQ=255)                                                                                                                                                                                                                              tAGCGTATCGTGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGt          >  2:59013/1‑251 (MQ=255)                                                                                                                                                                                                                               ggggtttCGTGCGGTGGGTGACCAGTTGAACCCAGGCGAATAGGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTAAAAGAGAGCGTGAAAGGGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTTCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGtt         <  2:502576/245‑1 (MQ=255)                                                                                                                                                                                                                               agtgtATCGGGCGCTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCAGCACCGCGCATCCGGCTAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGGGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGTTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGt          <  1:379601/246‑1 (MQ=255)                                                                                                                                                                                                                                       gtacgcTGCGTGACCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGGTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCtttt  >  2:165200/4‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             CCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTT  >  NC_000913/4579‑5058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑