BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,501	C→T	A303A (GCG→GCA)	fecC ←	ferric citrate ABC transporter membrane subunit FecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,501	0	C	T	99.2%	396.5 / NA	127	A303A (GCG→GCA)	fecC	ferric citrate ABC transporter membrane subunit FecC
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (63/61); minor base G (1/0); total (66/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTG  >  NC_000913/4512254‑4512741

cgcgGCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAgtgcg                                                                                                                                                                                                                                               >  2:140590/1‑251 (MQ=255)

   ggCAGTCGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCCGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGtgcgcgg                                                                                                                                                                                                                                            >  2:31580/1‑250 (MQ=255)

         cGGTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAg                                                                                                                                                                                                                                       <  1:588921/250‑1 (MQ=255)

          ggTACTCCATCAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCATGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTa                                                                                                                                                                                                                                     <  2:109147/251‑1 (MQ=255)

             aCTCCATCAATACATAATAATGCTCGCGTACGACATGCCAGTCGGTCAGCAGCGCGCGCAACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCACGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCACTCAGCGCAAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGAGCGCGTGCGAGTACAt                                                                                                                                                                                                                                  >  1:331589/1‑251 (MQ=255)

                   ccAATACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAAGCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCca                                                                                                                                                                                                                            <  2:154806/250‑1 (MQ=255)

                     aaTACATAATAATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCcagc                                                                                                                                                                                                                          >  2:525211/1‑251 (MQ=255)

                         aataataATGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCagcagc                                                                                                                                                                                                                       >  2:348679/2‑250 (MQ=255)

                            aataatGCCCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCGGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCa                                                                                                                                                                                                                   <  2:136319/251‑1 (MQ=255)

                               aatGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCg                                                                                                                                                                                                                >  1:450141/1‑251 (MQ=255)

                                atGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGt                                                                                                                                                                                                               <  1:137901/251‑1 (MQ=255)

                                atGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGAGCGAGTACATCTGCCAGCAGCATCAGCGt                                                                                                                                                                                                               >  2:261230/1‑251 (MQ=255)

                                 tGCTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATCTGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGCAAATAACCAGAGCAATTTTCATCCTCGCCTCCTCACAAGCCAGCCATAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGACAGATCTCCGGGGAAGGCCAGTGCGCGGGCGGGTACCTCGGCCAGCAGCATCAGCGTg                                                                                                                                                                                                              >  2:14459/1‑251 (MQ=255)

                                  gctcgcggccGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCAGATGGAGGGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTgg                                                                                                                                                                                                             <  2:167986/243‑1 (MQ=255)

                                  gcgcgcgtCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTgg                                                                                                                                                                                                             <  1:104809/245‑1 (MQ=255)

                                   cTCGCGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGc                                                                                                                                                                                                            >  1:563810/1‑251 (MQ=255)

                                     cgcgtCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCATCACGGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGccc                                                                                                                                                                                                          <  1:614688/248‑1 (MQ=255)

                                       cgtCCGGCCTGCCAGTCGGGCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGccccc                                                                                                                                                                                                        <  2:76589/250‑1 (MQ=255)

                                            ggCCTGCCAGTCGGTCAGCAGCGCGCTCCACGGCACGGGGATCACTCCCATATGGAATGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGGGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCcagcag                                                                                                                                                                                                   <  1:379218/251‑1 (MQ=255)

                                              ccTGCCAGGCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAAGTTGCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAgcagca                                                                                                                                                                                                 <  1:261811/251‑1 (MQ=255)

                                                 gCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCCCTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGc                                                                                                                                                                                              >  2:310947/1‑251 (MQ=255)

                                                 gCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCAt                                                                                                                                                                                                <  2:123410/249‑1 (MQ=255)

                                                    aGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTc                                                                                                                                                                                            >  1:447862/1‑250 (MQ=255)

                                                     ggcGGTCAGCAGCGCGCGCCACGGCACGGGTATCACTCCCATATGGAGTGATAACACCTCACAGCCCTCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTGTCATCCTCGCCTCCGCACAAGCCAGACACAGCAAGAGCTGCCTATCAGCGCCAGCACTGCGCCTGCTGGCAAATCTCCGGGGAAGGCCTGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTTGCCTCCAGCAGCATGCTc                                                                                                                                                                                            <  2:596799/247‑1 (MQ=255)

                                                     gTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCAc                                                                                                                                                                                          <  1:96959/251‑1 (MQ=255)

                                                        gggcagcagCGCGCGCCACGTCACGGGGATCACTCCCATATGGAGTGATAACATCGCGCAGCCCTCCATGGCAAGAGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGACATCACGGCGCCTGCGGGCAGATCTCCGTGGAAGGCCAGGGCGCATGCGTGTACATCTACCAGCAGCATCAGCGTGTCCCCAAGCATCATGCTCACTg                                                                                                                                                                                        >  2:573812/4‑250 (MQ=255)

                                                        ggTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTgg                                                                                                                                                                                       >  1:442965/1‑251 (MQ=255)

                                                           cagcagCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGc                                                                                                                                                                                      >  2:7106/1‑249 (MQ=255)

                                                              cagcGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTAc                                                                                                                                                                                 <  1:425977/251‑1 (MQ=255)

                                                                       ccACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTg                                                                                                                                                                        <  1:475266/251‑1 (MQ=255)

                                                                        cacggcacggGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTg                                                                                                                                                                        >  1:599281/1‑250 (MQ=255)

                                                                        cacggcacggGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGa                                                                                                                                                                       <  1:45325/251‑1 (MQ=255)

                                                                           ggcacggGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCg                                                                                                                                                                    <  1:178079/251‑1 (MQ=255)

                                                                                 gggATCCCGCCCATATGTAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTGCATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATTCTCACTGGCATTACGTTTCGCTGATCGAAGCCt                                                                                                                                                              <  2:411956/251‑1 (MQ=255)

                                                                                  ggATCACTCCCATATGGAGTGATACCAGCGAACAGCCCGCCAGGGCAAGGGTGATGAAAATAAACAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAATGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCCGCAGAATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTg                                                                                                                                                             >  2:481662/1‑251 (MQ=255)

                                                                                    aTCACTCCCATATGGAGTGATACCAGCGCACAGCCCGCCAGGGCAAGCGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCCATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCCGCACCAGCCTGGCCCCCAGCTGCAAGCTCACTGGCAGTACGTTGAGCTGATCGAAGCCTGcc                                                                                                                                                           >  2:356704/1‑251 (MQ=255)

                                                                                    aTCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGcc                                                                                                                                                           <  1:31580/251‑1 (MQ=255)

                                                                                              tatGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAgcgcg                                                                                                                                                 >  2:447035/1‑251 (MQ=255)

                                                                                                tGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATGACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGcc                                                                                                                                               >  1:36606/1‑251 (MQ=255)

                                                                                                           cAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCAc                                                                                                                                    >  1:452286/1‑251 (MQ=255)

                                                                                                           cAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGAACATCTGCCAGCAGCATCAGCGTGGCACCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCACAGAAGCGCGCCAGATGTGacac                                                                                                                                    >  1:53702/1‑251 (MQ=255)

                                                                                                            aGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCAcc                                                                                                                                   >  1:286030/1‑251 (MQ=255)

                                                                                                               gcccaagccGCCAGGGCAAGGGGGATGAAAACAACCAGCGCAATTTTCATCCTCGCCTCCTCACCAGCCAGACAAACCAAGTGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGAAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCGCACTGGCAGTCCGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCAAcagc                                                                                                                                <  2:21251/244‑1 (MQ=255)

                                                                                                               gcACATCCCGCCAGGGCAAGGGTGATTAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAATCAAGGGCTGCTAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCTCGTGCGAGTACATCTGCCAGCAGCATCAGCGTTGCCCCCAGCAGCATGCTCTCTGGCAGTGCGTTGCGCTGATTGAATTCGGCTCAGAAGCGCGCCAGATGTGGCACcagt                                                                                                                                <  2:563107/251‑2 (MQ=255)

                                                                                                               gcACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTACGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACcagc                                                                                                                                <  2:462492/251‑1 (MQ=255)

                                                                                                                 acaGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACcagc                                                                                                                                >  1:121927/1‑249 (MQ=255)

                                                                                                                   aGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGCCAAAGCAAGGCCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGAc                                                                                                                            >  1:562738/1‑251 (MQ=255)

                                                                                                                   aGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCCGAAGCGCGCCAGATGTGGCACCAGCAGAc                                                                                                                            >  2:193210/1‑251 (MQ=255)

                                                                                                                    gCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCCCAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCTCGCCAGATGTGGCACCAGCAGAcc                                                                                                                           <  2:339340/251‑1 (MQ=255)

                                                                                                                         ccAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATa                                                                                                                       >  2:266320/1‑250 (MQ=255)

                                                                                                                            gggCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCACTCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGc                                                                                                                                                                                      >  2:516167/1‑184 (MQ=255)

                                                                                                                            gggCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGc                                                                                                                                                                                      <  1:516167/184‑1 (MQ=255)

                                                                                                                            gggCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACg                                                                                                                   >  1:62499/1‑251 (MQ=255)

                                                                                                                             ggCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCATCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGc                                                                                                                  <  1:161351/251‑1 (MQ=255)

                                                                                                                             ggCAAGGGGGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGc                                                                                                                  <  1:499806/251‑1 (MQ=255)

                                                                                                                                 aGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCAcc                                                                                                              <  2:335498/251‑1 (MQ=255)

                                                                                                                                         gaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGc                                                                                                      >  2:342993/1‑251 (MQ=255)

                                                                                                                                          aaaaTAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGGGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGcc                                                                                                     <  1:604584/251‑2 (MQ=255)

                                                                                                                                            aaTAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCCCCGGACCTGccac                                                                                                   <  1:456579/251‑4 (MQ=255)

                                                                                                                                                aCCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTg                                                                                               >  2:23356/1‑251 (MQ=255)

                                                                                                                                                aCCAGCGCAATTTTCATCCTCGCCTCCCCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCAGCAGCGTGGCCCCCAGCAGCAGGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCATATGTGGCACCAGCATACCGATAAACGCCACCGGACCTGccgcgctg                                                                                               <  2:85219/251‑8 (MQ=255)

                                                                                                                                                   aGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGacg                                                                                            <  1:289729/251‑1 (MQ=255)

                                                                                                                                                      gcAAGTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGAGCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGacgcac                                                                                         <  1:589035/251‑1 (MQ=255)

                                                                                                                                                             tCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCacca                                                                                   >  2:123405/1‑250 (MQ=255)

                                                                                                                                                                 ccTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACACCACCGGACCTGCCACGCTGACGCACGCACCAACCAg                                                                              <  2:81565/251‑1 (MQ=255)

                                                                                                                                                                    ggccgccccacaAGCCAGACAAAGCAAGGGCGGCCAACCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCTCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAag                                                                           <  2:532507/243‑1 (MQ=255)

                                                                                                                                                                       ctcctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAagc                                                                          >  1:127167/1‑249 (MQ=255)

                                                                                                                                                                       ctcctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAagc                                                                          >  1:289261/1‑249 (MQ=255)

                                                                                                                                                                        tcctcACAAGCCAGACAACGCAAGGGCTGCCAATCAGCGCCAGCCCTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATTAGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAagcagc                                                                       >  2:440805/1‑251 (MQ=255)

                                                                                                                                                                        tcctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAagcagc                                                                       >  1:311821/1‑251 (MQ=255)

                                                                                                                                                                         cctaacaAGCCAGACAAAGCATGGGCTGCCAATCAGCGCCAGCACGGCGCCTGCGGGCAGAGCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCTAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGCCCGATAAAGGCCTCCGTACCTGCCACGCTGACGCACGCACCAACCAGAAgcagca                                                                      <  2:504188/247‑1 (MQ=255)

                                                                                                                                                                           tcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCCCCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACt                                                                    >  2:80570/1‑251 (MQ=255)

                                                                                                                                                                            cacaAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCAc                                                                     <  1:52294/249‑1 (MQ=255)

                                                                                                                                                                                   cAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATAtt                                                            >  1:293012/1‑251 (MQ=255)

                                                                                                                                                                                    ataCAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCGTCGTGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATtg                                                           <  1:356106/249‑1 (MQ=255)

                                                                                                                                                                                    aGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCACAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTAACGCACGCACCAACCAGAAGCAGCACTAACATATtg                                                           >  1:209262/1‑251 (MQ=255)

                                                                                                                                                                                         aaGCAAGGGCTGCCAATAAGAGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGAc                                                      >  1:186785/1‑251 (MQ=255)

                                                                                                                                                                                          aGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGAcc                                                     >  1:537005/1‑251 (MQ=255)

                                                                                                                                                                                           gCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGAcc                                                     >  1:25185/1‑250 (MQ=255)

                                                                                                                                                                                            cAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCaa                                                   <  1:513243/251‑1 (MQ=255)

                                                                                                                                                                                                 gCTGCCAATCAGCGCCAGCACGGCGCCGGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGGGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGAGCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTa                                              <  2:293012/251‑1 (MQ=255)

                                                                                                                                                                                                   tGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCACCCAGAAGCAGCACTAACATATTGATGACCAAACGTAGc                                            >  2:450967/1‑251 (MQ=255)

                                                                                                                                                                                                     ccAATCAGCGCCAGCACTCCGCCTCCGGGCAGATCTCCGGGGAAGGCCCGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGCTGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCCCGCCCCAACCAGAAGCAGCACTACCACATTGATGACCAAACGTATCCt                                          >  2:69708/1‑251 (MQ=255)

                                                                                                                                                                                                          cAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCCGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTc                                      >  1:574225/1‑250 (MQ=255)

                                                                                                                                                                                                          cAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTc                                      <  1:233962/250‑1 (MQ=255)

                                                                                                                                                                                                          cAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCa                                     <  1:265622/251‑1 (MQ=255)

                                                                                                                                                                                                          cAGCGCCAGCACGGCGCCTGCGGGCAGACCCCAGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATGGATGACCAAACGGAGCCTCGTc                                      <  2:260630/250‑1 (MQ=255)

                                                                                                                                                                                                                cAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGtcc                                >  1:559251/1‑248 (MQ=255)

                                                                                                                                                                                                                    aCTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTcc                           >  1:174565/1‑251 (MQ=255)

                                                                                                                                                                                                                     ccgcgcCTGCGGGCAGATCCCCGGGGAAGGCCAGTGCGCGTGCGAGTACACCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCCCCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTccc                          <  2:24056/249‑1 (MQ=255)

                                                                                                                                                                                                                      ggcgcCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCa                         <  2:62499/250‑1 (MQ=255)

                                                                                                                                                                                                                       gcgcCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTccc                          <  1:461855/249‑1 (MQ=255)

                                                                                                                                                                                                                               gggaaGATCTACGGGGAAGGCCAGTGCGCGTGCGGGTACAGCTGCCAGCAGCAACAGCGTGGCCCCCAGCAGCATGCGCACTGGCAGAACGTGACACTGATCGAAGCCTGCCCAGAGGCGCGCCAGATGTGGCACCAGCAGACCGATAACCGCCACCGGACCTGCAACGCGGACGCACGCACCAACAAGCAGCAGCACGAGCATATTAAAGACCAAACGACACCTAGTCAGGTTACCGCCCAGCGTaag                  >  2:426466/5‑247 (MQ=255)

                                                                                                                                                                                                                                  cAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCgg             >  1:435116/1‑251 (MQ=255)

                                                                                                                                                                                                                                   aGATCTCCGGGGAAGGCCAATGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCCGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCCGGTTCCATCCCAGCGTATGGGCggg            >  2:72156/1‑250 (MQ=255)

                                                                                                                                                                                                                                     aTCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGc          >  2:178860/1‑251 (MQ=255)

                                                                                                                                                                                                                                        cccGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGt       <  1:525211/250‑1 (MQ=255)

                                                                                                                                                                                                                                         ccGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCt         <  1:348679/248‑1 (MQ=255)

                                                                                                                                                                                                                                           ggggAAGGCCGGTGCGCGTTCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGACCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGc    <  2:563810/251‑1 (MQ=255)

                                                                                                                                                                                                                                             ggAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTg  >  1:229660/1‑251 (MQ=255)

CGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTG  >  NC_000913/4512254‑4512741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`