Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,424	G→C	pseudogene (228/255 nt)	insO →	IS911B regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,424	0	G	C	100.0%	214.8 / NA	68	pseudogene (228/255 nt)	insO	IS911B regulator fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (37/31);  total (37/31)

CAGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACT  >  NC_000913/4507186‑4507663                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                caGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGgag                                                                                                                                                                                                                                     <  2:46706/251‑1 (MQ=255)  aGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGgaga                                                                                                                                                                                                                                    <  1:549206/251‑1 (MQ=255)  aGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTGGGCCTTGCCACAATGACAAGATGTGTCAAGCAACTGCGTGATGAGCGGCGGGGCAAAACACCAAAAGCCGCTCCGATAACACCAGAACAAATCCAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGgaga                                                                                                                                                                                                                                    <  1:361973/251‑1 (MQ=21)      gCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATg                                                                                                                                                                                                                                 >  1:588519/1‑250 (MQ=255)              gAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTaa                                                                                                                                                                                                                        <  1:79498/251‑1 (MQ=255)               aaaaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTaa                                                                                                                                                                                                                        >  2:474897/1‑250 (MQ=255)               aaaaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTaa                                                                                                                                                                                                                        >  1:414735/1‑250 (MQ=255)                 aaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACACCGCATTGAAATGGAGAATGAAATATTaaaa                                                                                                                                                                                                                      >  1:388521/1‑250 (MQ=255)                 aaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTg                                                                                                                                                                                                                                             <  1:139427/227‑1 (MQ=255)                 aaaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTg                                                                                                                                                                                                                                             >  2:139427/1‑227 (MQ=255)                  aaaaGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAATACCAGAACAAATCGAAATACGTAAGCTGAGGAAAACGCTACAACGCATTGAAATGGAGAATGAAATATTaaaa                                                                                                                                                                                                                      >  1:527022/1‑249 (MQ=255)                         aTTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGGTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGAGTCAAACAACTGCGTGATGAGCGTCAGGTCAAAACACCAAAAGTCTCTCCGATAACACCAGAACAAATCGAAATAGTTAAGCGGAGGAAAAAGCTACAACTCAGTGAAATGGAGAAGGAAATATTAAAAAGGCTaccg                                                                                                                                                                                                             >  2:604436‑M2/1‑247 (MQ=255)                           ttCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcg                                                                                                                                                                                                           <  2:485339‑M2/251‑7 (MQ=255)                           ttCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcg                                                                                                                                                                                                           <  1:287323‑M2/251‑7 (MQ=255)                                   agagTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAATCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgat                                                                                                                                                                                                   >  1:403220‑M2/1‑237 (MQ=255)                                   agagTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGACAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgat                                                                                                                                                                                                   <  2:176944‑M2/251‑15 (MQ=255)                                            aCGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactc                                                                                                                                                                                          >  2:506745‑M2/1‑228 (MQ=255)                                             agcgaATCCGCTCAACTGGATGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactcc                                                                                                                                                                                         <  2:350370‑M2/250‑25 (MQ=255)                                               cgAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccct                                                                                                                                                                                       >  1:605347‑M2/1‑225 (MQ=255)                                                gAATCCGCTTAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCCGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactacctg                                                                                                                                                                                      <  2:511083‑M2/251‑28 (MQ=255)                                                gAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACACTGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctg                                                                                                                                                                                      <  2:255084‑M2/251‑28 (MQ=255)                                                 aaTCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctga                                                                                                                                                                                     >  2:22261‑M2/1‑223 (MQ=255)                                                 aaTCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctg                                                                                                                                                                                      <  1:422387‑M2/250‑28 (MQ=255)                                                      gCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACACCGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagt                                                                                                                                                                                <  1:404792‑M2/251‑34 (MQ=255)                                                             tGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGAGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAAAAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGAAAGCTGAGGAAAAAGCTACAACGCATTGCAATGGAGAATGAACTATAACAAAGGCTaccgcgctcttgatgtaagactacatgaacagttct                                                                                                                                                                             <  1:250543‑M2/247‑37 (MQ=255)                                                              gtttgttgacCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagacgccctgaacagttctcgata                                                                                                                                                                        >  2:426107‑M2/3‑210 (MQ=255)                                                              ggTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCCAGCAACTGCGTGATGAGCGTCAGGGCAAAACACAAAAAGCCTCTCCGATAAAACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGAAGAATGAAATATTAAAAAGGCTacggcgctcttgatgtcagactccctgaacagttctcgat                                                                                                                                                                         <  1:413910‑M2/250‑41 (MQ=255)                                                                       acaGAAATACACGGTGGCAGATGCCGCGACAGCTATGGCTGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAACACACCACAAGCCTCTCCGATAACACCAGATCAAATCGACATACGTAAGCTGAGGAAAACGCTACCACGCATTGAAATGGAGACTGATATATTAAAAAGGCTaccgcgctattgatgtcagactccctgaacagttctcgataatcgggta                                                                                                                                                                <  2:232325‑M2/249‑50 (MQ=255)                                                                              tACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagcg                                                                                                                                                        <  2:218425‑M2/251‑58 (MQ=255)                                                                               ccacgGTGGCAGTGGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGTGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACTCCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctctttatgtcagactccctgaacatttctcgataatcgggatactcagagc                                                                                                                                                       >  2:290460‑M2/2‑193 (MQ=255)                                                                               acacGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatggcagactccctgaacagttctcgagaaacgggaaactcagagc                                                                                                                                                       >  1:243744‑M2/1‑193 (MQ=255)                                                                                      ggCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACACGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGACTGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattat                                                                                                                                                <  1:554705‑M2/251‑66 (MQ=255)                                                                                      ggCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattat                                                                                                                                                <  1:212826‑M2/251‑66 (MQ=255)                                                                                        cAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcc                                                                                                                                              >  1:451476‑M2/1‑184 (MQ=255)                                                                                           aTGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgt                                                                                                                                           <  1:364518‑M2/251‑71 (MQ=255)                                                                                               cgcgAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtgggc                                                                                                                                       >  2:386442‑M2/1‑177 (MQ=255)                                                                                                   aaaGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacac                                                                                                                                   <  2:167961‑M2/251‑79 (MQ=255)                                                                                                       cTATGGATGTTGGCCTTTCAACAATGACAAGATGGGTCAAACAACTGCGTGATGCGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTAAAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgctgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcccactctg                                                                                                                               <  2:526989‑M2/251‑83 (MQ=255)                                                                                                         aTGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgcc                                                                                                                             >  2:396860‑M2/1‑167 (MQ=255)                                                                                                           ggATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctttggtcacactctgcca                                                                                                                            >  1:400114‑M2/1‑165 (MQ=255)                                                                                                              tGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtg                                                                                                                        <  2:289558‑M2/251‑90 (MQ=255)                                                                                                               gTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtg                                                                                                                        >  2:422387‑M2/1‑161 (MQ=255)                                                                                                                  ggCCTTTCCACAATGACAAGATGGGGCAAACAACTGCGTGATGAGCGTCAGGGAAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcctgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcatgatcctgtggtcacactctgccatgtgttcg                                                                                                                    >  2:155057‑M2/1‑158 (MQ=255)                                                                                                                        tCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaaaagttctcgataatcgggaaactcagagcgccttatcctgtggtcacactctgccatgtgatcggggttc                                                                                                              <  1:379054‑M2/251‑100 (MQ=255)                                                                                                                         ccACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttca                                                                                                             >  1:344557‑M2/1‑151 (MQ=255)                                                                                                                         ccACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttca                                                                                                             >  2:221380‑M2/1‑151 (MQ=255)                                                                                                                             aaTGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAACACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGCGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGACTaccgcgctcttgatgtcagactacctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgc                                                                                                         <  2:329953‑M2/251‑105 (MQ=255)                                                                                                                               tGACAAGATGGGTCAAACCACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgatactcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgc                                                                                                         <  1:162770‑M2/249‑105 (MQ=255)                                                                                                                                                 aacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATCACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactgga                                                                                      <  1:90080‑M2/250‑124 (MQ=255)                                                                                                                                                 aacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaa                                                                                     >  1:289558‑M2/1‑127 (MQ=255)                                                                                                                                                      cGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacgctctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaacc                                                                                >  1:78687‑M2/1‑122 (MQ=255)                                                                                                                                                          atgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatacttgaaaaaccgtcc                                                                            >  1:186983‑M2/1‑118 (MQ=255)                                                                                                                                                              gCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaa                                                                        >  2:390375‑M2/1‑114 (MQ=255)                                                                                                                                                                   aGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCCGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaacc                                                                   >  1:511083‑M2/1‑109 (MQ=255)                                                                                                                                                                            cacCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcag                                                          >  1:570957‑M2/1‑100 (MQ=255)                                                                                                                                                                                     ccTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgt                                                 >  1:175374‑M2/1‑91 (MQ=255)                                                                                                                                                                                          ccGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctgcagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtatt                                              >  1:565456‑M2/1‑86 (MQ=255)                                                                                                                                                                                             aTAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgc                                          <  1:419320‑M2/250‑168 (MQ=255)                                                                                                                                                                                              tAACACCAGAACAAATCGACATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaacccagacggcagacgggctgtattacgc                                          <  2:27374‑M2/249‑168 (MQ=255)                                                                                                                                                                                                 cacCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTCAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtc                                      <  1:422112‑M2/250‑172 (MQ=255)                                                                                                                                                                                                   ccAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcagg                                   >  1:475898‑M2/1‑77 (MQ=255)                                                                                                                                                                                                   ccAGAACAAATCGAAAGACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactcccggaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacgtcagacgggctgtattacgcagtcagg                                   >  2:281419‑M2/1‑77 (MQ=255)                                                                                                                                                                                                      gAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtac                                <  1:581680‑M2/251‑178 (MQ=255)                                                                                                                                                                                                           aaTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgag                           >  1:263840‑M2/1‑69 (MQ=255)                                                                                                                                                                                                            aTCGAAATACGGAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagc                          >  2:388521‑M2/1‑68 (MQ=255)                                                                                                                                                                                                                        aaGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatca              >  2:162770‑M2/1‑56 (MQ=255)                                                                                                                                                                                                                                  aaaaGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttc    >  1:95799‑M2/1‑46 (MQ=255)                                                                                                                                                                                                                                    aaGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcgg  >  1:118805‑M2/1‑44 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                CAGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACT  >  NC_000913/4507186‑4507663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑