Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,448,304	C→T	intergenic (+69/‑143)	ytfP → / → chpS	gamma‑glutamylamine cyclotransferase family protein YtfP/ChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,448,304	0	C	T	100.0%	185.4 / NA	61	intergenic (+69/‑143)	ytfP/chpS	gamma‑glutamylamine cyclotransferase family protein YtfP/ChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/36);  total (25/36)

TTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGC  >  NC_000913/4448058‑4448549                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ttATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGGAAAGAAACCATATGCATACGCCACCTTCGG‑TGGCCGTGGTTTTTTTCGAGACGCCCCGCATTCGGTTTTGAAATTTCCTc                                                                                                                                                                                                                                                   >  2:467868/1‑251 (MQ=255) ttATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCt                                                                                                                                                                                                                                                    <  1:449845/250‑1 (MQ=255)  tATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTGTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTc                                                                                                                                                                                                                                                   <  2:113921/250‑1 (MQ=255)        aTTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCttt                                                                                                                                                                                                                                             <  1:560159/250‑1 (MQ=255)        aTTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCttt                                                                                                                                                                                                                                             <  1:32752/250‑1 (MQ=255)        aTTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCttt                                                                                                                                                                                                                                             <  1:174566/250‑1 (MQ=255)              aaCGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCtttt                                                                                                                                                                                                                                      <  2:98252/251‑1 (MQ=255)                                  tGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTcc                                                                                                                                                                                                                    <  1:107674/249‑1 (MQ=255)                                       ccTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCt                                                                                                                                                                                                              >  1:83012/1‑250 (MQ=255)                                          tGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCCCCTTCGGGGGGC‑GTTGTTTTTTGCGGGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTAtt                                                                                                                                                                                                           >  2:432816/1‑250 (MQ=255)                                              cACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGc                                                                                                                                                                                                      <  1:439593/251‑1 (MQ=255)                                                  aGGGGCGGAGCATACGCTCGCCAGTTGATTCAGACGCCGTCCGGGAGAGCCTGGATGTAAGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGTTTAGGAACCATATGCATACGCCACCTTAGGGGGGC‑GTTGTTTTTTGCGGGTCGACTCGCATTCTTTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTTACGCAtc                                                                                                                                                                                                   >  2:435304/1‑249 (MQ=255)                                                     ggCGGTGAATACGCGCGCCAGTTGATTCATACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATaaaaa                                                                                                                                                                                               <  2:431865/251‑1 (MQ=255)                                                     ggCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATaaaaa                                                                                                                                                                                               <  1:145204/251‑1 (MQ=255)                                                         gTGAATACGAGCGCCAGTGGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTACTTGAAAGCAGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCCCCTTCGGGTGGC‑GTTGTTTTTTGCGCGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAAct                                                                                                                                                                                            >  2:560149/1‑250 (MQ=255)                                                                   gcgcCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCAtt                                                                                                                                                                                 <  2:527658/251‑1 (MQ=255)                                                                                  gACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAggg                                                                                                                                                                  >  2:587750/1‑251 (MQ=255)                                                                                  gACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAggg                                                                                                                                                                  >  2:584416/1‑251 (MQ=255)                                                                                   acgcagttcGGGAGTGCATGGATGTACGGTTATCAACTACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAgggg                                                                                                                                                                 <  2:164618/243‑1 (MQ=255)                                                                                        gTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTaaaa                                                                                                                                                            <  2:6174/251‑1 (MQ=255)                                                                                          aCGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAAc                                                                                                                                                           <  1:111738/250‑1 (MQ=255)                                                                                            gggAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCCGGGGTAAAACGtt                                                                                                                                                        >  2:493252/1‑251 (MQ=255)                                                                                                  gCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGt                                                                                                                                                  <  1:201897/251‑1 (MQ=255)                                                                                                      ggATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGc                                                                                                                                               >  1:220970/1‑250 (MQ=255)                                                                                                          gTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGt                                                                                                                                          <  1:51164/251‑1 (MQ=255)                                                                                                                 tATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTta                                                                                                                                    <  2:11585/250‑1 (MQ=255)                                                                                                                     aaCGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                               <  1:44604/251‑1 (MQ=255)                                                                                                                           ccGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCGCCGAGCCGCTTTCCATATCTAGTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGtata                                                                                                                         <  2:208820/251‑1 (MQ=255)                                                                                                                               cGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACt                                                                                                                      <  1:578875/250‑1 (MQ=255)                                                                                                                                     aTTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCGCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGGTTCCTGTAGCACCGTGAGTTATTCTTTGTATAACTTAaggag                                                                                                               <  2:410644/251‑1 (MQ=255)                                                                                                                                        aaaGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGt                                                                                                             <  1:345045/250‑1 (MQ=255)                                                                                                                                        aaaGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTg                                                                                                            <  1:244517/251‑1 (MQ=255)                                                                                                                                            cTAATTGAAAGCGGCGACTGGATAGACAGGGATACGTAACCATATGCCTACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGAAGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAACTGCGCAGGGGTAACACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGc                                                                                                           >  2:267451/1‑248 (MQ=255)                                                                                                                                              aaTTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGGTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGAt                                                                                                       <  2:98513/250‑1 (MQ=255)                                                                                                                                                    aagcgggcgcTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTATTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAtt                                                                                                <  1:467868/242‑1 (MQ=255)                                                                                                                                                               ggTTAGACAGGTATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAg                                                                                     <  2:538342/251‑1 (MQ=255)                                                                                                                                                               ggTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTTGCATTCACAAATGCGCAGGGGTAAAACGGTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATaaaa                                                                                       <  1:267451/249‑1 (MQ=255)                                                                                                                                                                  tAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGAt                                                                                   <  2:101496/250‑1 (MQ=255)                                                                                                                                                                  tAGACAGGGATAAGAAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAACAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATg                                                                                  >  2:74612/1‑251 (MQ=255)                                                                                                                                                                   aGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTTTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATGCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                 <  2:60295/251‑1 (MQ=255)                                                                                                                                                                          gATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTACTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAAc                                                                           >  1:298127/1‑250 (MQ=255)                                                                                                                                                                               gTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGcc                                                                     >  2:578043/1‑250 (MQ=255)                                                                                                                                                                               gTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGc                                                                      >  1:84248/1‑250 (MQ=255)                                                                                                                                                                                         gCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGCTTTGTAATTTACTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAAATCTACTGGCATTCACAAATGCGAAGGGGTAAAACGTTTCCTGTTGCACCGTGCGTTATACTTTGAATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAACGATGGGGGAACAGTGCAggcattgtc                                                            >  1:627553/1‑243 (MQ=255)                                                                                                                                                                                                    cTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCATGTAGCACCATGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTCTTACCATAAAAAGAAGGGGGAACAGAGCAGGTATGGGCGTTCCCAATATc                                                >  1:348947/1‑251 (MQ=255)                                                                                                                                                                                                     atCGGGAGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCAAAAATGCGCAGGGGTAAACCGTTTCCTGTAGCACCGTGAGATATAATTTGTATCACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAAAAGTGAAGGTATGGTCATTCCCAATATCg                                               >  2:446030/2‑251 (MQ=255)                                                                                                                                                                                                       cGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGt                                              >  2:142195/1‑250 (MQ=255)                                                                                                                                                                                                                 ttgttTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGa                                     >  1:177183/1‑250 (MQ=255)                                                                                                                                                                                                                  ggttTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGGGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaa                                    <  2:219986/249‑1 (MQ=255)                                                                                                                                                                                                                   gttTTTTGCGAGACTACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaact                                  <  2:470690/251‑1 (MQ=255)                                                                                                                                                                                                                    ttttttGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaactt                                 >  1:154555/1‑251 (MQ=255)                                                                                                                                                                                                                       tttGCGAGACGTCTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGTTCATTCCCAATATCGTAATGAAAGaacttaa                               <  1:178684/250‑1 (MQ=255)                                                                                                                                                                                                                       tttGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaa                               <  1:304142/250‑1 (MQ=255)                                                                                                                                                                                                                         tGCGAGACGACTCGCATTCTGTTTTGTAATTTCCTCCCCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaact                             <  2:143948/250‑1 (MQ=255)                                                                                                                                                                                                                             agaCGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTAc                          >  1:148463/1‑249 (MQ=255)                                                                                                                                                                                                                             agaCGACTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTAc                          >  1:279550/1‑249 (MQ=255)                                                                                                                                                                                                                              gacgacTCGCATTCTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGc                       >  1:375881/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTGTTTTGTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt          >  2:147828/1‑251 (MQ=255)                                                                                                                                                                                                                                            tGTTTTGTAATTTCCGCACATTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCTTAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGTAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAAATTAACTTACAGCCGGTGCAGAGCg           >  2:521889/1‑249 (MQ=255)                                                                                                                                                                                                                                                  gTAATTTCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGgc    <  2:3487/250‑1 (MQ=255)                                                                                                                                                                                                                                                    aaTTTCCTCACCTTCTGCTTTTCTCCCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAGCTCTGCTGGCACTCACAAAGGCGCAGGGGTAAAACGTTTCCTGAAGCAGCGTGAGTTATTCTTTGTATAACTTAAGGAGTTGCAGATGCGTATTTCGATAAAAAGATATGGGAACAGTGCAGTTATGGTCATTCCCAAGATCGTAATGAAAGATCTTAACTTACAGCCGGGGCAGAGCGTGGAGgcgc  <  1:616710/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TTATCGTATTGACAACGCCACGCTGGCCGAACTTGATGCCTTGCGCACCAGGGGCGGTGAATACGCGCGCCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGC‑GTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGC  >  NC_000913/4448058‑4448549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑