Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,439,578	G→A	intergenic (‑316/‑9)	ytfJ ← / → ytfK	PF09695 family protein YtfJ/stringent response modulator YtfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,439,578	0	G	A	100.0%	168.2 / NA	53	intergenic (‑316/‑9)	ytfJ/ytfK	PF09695 family protein YtfJ/stringent response modulator YtfK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/22);  total (31/22)

CGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGT  >  NC_000913/4439335‑4439817                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                cGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAAc                                                                                                                                                                                                                                          >  1:468677/1‑251 (MQ=255)    aTTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATGCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGGGGCACCGCACAGGCGTAATACGCAGCATAAGATAACAAt                                                                                                                                                                                                                                       <  2:208458/251‑1 (MQ=255)      ttCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATg                                                                                                                                                                                                                                      >  1:186712/1‑250 (MQ=255)             aaGTGTGATGCCAGTTTGCGGGCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGTATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATTAAAAtttt                                                                                                                                                                                                                              <  2:226263/251‑1 (MQ=255)              aGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATCACAATGAAAATTTTc                                                                                                                                                                                                                             >  1:606899/1‑251 (MQ=255)                        cAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTAc                                                                                                                                                                                                                    >  1:515340/1‑250 (MQ=255)                        cAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAAAGGTACa                                                                                                                                                                                                                   <  2:140768/251‑1 (MQ=255)                                         cacaAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCg                                                                                                                                                                                                  >  1:77412/1‑251 (MQ=255)                                                               tgCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTcc                                                                                                                                                                            >  1:618501/1‑251 (MQ=255)                                                               tgCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTcc                                                                                                                                                                            >  1:485226/1‑251 (MQ=255)                                                                    ataCTGAAGACCGCGCTGCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCATCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACACCCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGa                                                                                                                                                                       <  2:483481/251‑1 (MQ=255)                                                                          aaGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGTCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACg                                                                                                                                                                     >  1:405103/1‑247 (MQ=255)                                                                          aaGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGTCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACGTCAGGTGGCGAAGTACGTAAAGATCCTGGTCCGTGGACg                                                                                                                                                                     <  2:405103/247‑1 (MQ=255)                                                                                   cTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCa                                                                                                                                                          >  1:300408/1‑249 (MQ=255)                                                                                      cGGTAAAAAGATAATTCTGAATCATTGTAACCTTTAGGTAAAAAAAGTTATACACGGTGGAAACATTGCCCGTATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGTCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGAc                                                                                                                                                     >  2:426209/1‑251 (MQ=255)                                                                                          aaaaaGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGAAAAGATCATGTTCCGTGGACGGTTATACATCAAGGACGTTg                                                                                                                                                 >  2:119728/1‑251 (MQ=255)                                                                                              aGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGgcgc                                                                                                                                             >  1:458817/1‑251 (MQ=255)                                                                                                  aaTTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAACCATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTAGGCCGATTATGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCtttt                                                                                                                                         >  1:240563/1‑251 (MQ=255)                                                                                                        gAATAATTGTAACCTTTAGGGAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGCCTAAACTCACTAAGCATTAAAATTTGAGAATCCTACTAGTTGGGCCGATTGTGGCACCGCACAGGCGAACTACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGGAAAACCCCCTTCAGGTGGCGAAGTACGTAACGATCCTGTTCCGTGGACGGTTATAAAGCCAGGACGTTGGCGCTTTTGAATTc                                                                                                                                   >  2:208332/1‑251 (MQ=255)                                                                                                            aatTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATa                                                                                                                               <  1:72032/251‑1 (MQ=255)                                                                                                             atTGTAACCTTGAGGTAAAAAAAGTTAGACGCGGTGGAAACAGTGCCCGGATAGTCTATAGTCACGAAGCAGGAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAAGACTCAGCAGAAGATAACAATGAAAATTAGCCAACGGGGCAACCCACTGCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGGGGACGGTTATACATCACGGACGTCGGCGTTTTTGAATTCGATaa                                                                                                                              <  2:307478/251‑1 (MQ=255)                                                                                                              ttGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATa                                                                                                                               >  2:398957/1‑249 (MQ=255)                                                                                                               tGTAACCTTTAGGGAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGAGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGCACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAgg                                                                                                                            >  2:455399/1‑251 (MQ=255)                                                                                                                gTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAACCATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCCGGTGGCGAAGTACGTAAAGCTACTGTTCCGTGGACAGTTATACATCAAGGACGTTGGAGCTTTTGCATTCGATAAggg                                                                                                                           >  2:5584/1‑251 (MQ=255)                                                                                                                gTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAggg                                                                                                                           >  1:141344/1‑251 (MQ=255)                                                                                                                gTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAgg                                                                                                                            <  2:618501/250‑1 (MQ=255)                                                                                                                  aaCCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGt                                                                                                                          >  2:624752/1‑250 (MQ=255)                                                                                                                       ttAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGAt                                                                                                                     <  1:351806/250‑1 (MQ=255)                                                                                                                          ggTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCtt                                                                                                                 <  1:492398/251‑1 (MQ=255)                                                                                                                             aaaaaaaGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATc                                                                                                              <  2:254654/251‑1 (MQ=255)                                                                                                                                aaaaGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATccc                                                                                                            >  2:544500/1‑250 (MQ=255)                                                                                                                                      tataCGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTg                                                                                                     >  1:441812/1‑251 (MQ=255)                                                                                                                                       ataCGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTg                                                                                                     >  1:328957/1‑250 (MQ=255)                                                                                                                                          cgcgGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACATCCCTCTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAgt                                                                                                 <  2:424879/251‑2 (MQ=255)                                                                                                                                                         gCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGTCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGTGTAAGATTCTTATCCCAAAAGTGAAGGTTAAGCTGCATTTg                                                                                   <  1:202931/250‑1 (MQ=255)                                                                                                                                                            cGGATAGTCTATAGTCACGAAGCATTAAAATTTGCGCCTCATAATATTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACATCCCTCTTCAGTTGGCGAAGTACGTAAAGATCCTGTTCCGTGGTCGGTTATACATCAAGGACGTTGTCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAg                                                                               <  1:608217/251‑1 (MQ=255)                                                                                                                                                                    cTATAGGCACTAAGAATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGCTAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTcc                                                                       >  2:353933/1‑251 (MQ=255)                                                                                                                                                                                   aTTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACGTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTGGGCGCTTTTGAATTCGATAAGTGTAAGATGCTTAGCCCAAAAGTGAAGGATAAGCTGCATTGGTCAGTGATGTCCGAAGGTAACCGTCAg                                                        <  2:75700/251‑1 (MQ=255)                                                                                                                                                                                      aaaaTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGtt                                                     <  2:141344/251‑1 (MQ=255)                                                                                                                                                                                        aaTTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTAt                                                   >  1:132019/1‑251 (MQ=255)                                                                                                                                                                                             gcgcCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTc                                              >  2:364868/1‑251 (MQ=255)                                                                                                                                                                                                ccTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGc                                           >  1:299026/1‑251 (MQ=255)                                                                                                                                                                                                    ataataGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCaaa                                        >  1:465255/1‑250 (MQ=255)                                                                                                                                                                                                            tGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATACCAATGAAAATTTTCCAACCGTACAACCCACTTCAGGAGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGCTAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATgg                               >  1:413284/1‑251 (MQ=255)                                                                                                                                                                                                               gCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCtt                            >  1:208314/1‑251 (MQ=255)                                                                                                                                                                                                                      gtgGCACCGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaa                      >  2:211305/1‑250 (MQ=255)                                                                                                                                                                                                                             cGCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctat              <  2:300408/251‑1 (MQ=255)                                                                                                                                                                                                                              gCACAGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatg             <  1:401104/251‑1 (MQ=255)                                                                                                                                                                                                                                acaGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGCGATGGGTTAACCaaagtgctatgca           <  2:25883/251‑1 (MQ=255)                                                                                                                                                                                                                                 caGGCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcag          <  1:398957/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGTAATACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTGTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGGCTGCAAACAGAGATGGCTTAACCaaagtgctatgcagta        <  2:606899/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaTACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcagtaaaaag   <  1:426209/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTACTCAGCAGAAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGATCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCaaagtgctatgcagtaaaaagt  >  2:5356/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                CGAATTTCCCGCAAGTGTGATGCCAGTTTGCGGTCAAGCGCACAAATCATATGAAAAATGAATGCTTATACTGAAGACCGCGCTTCGGTAAAAAGATAATTCTGAATAATTGTAACCTTTAGGTAAAAAAAGTTATACGCGGTGGAAACATTGCCCGGATAGTCTATAGTCACTAAGCATTAAAATTTGCGCCTCATAATAGTTGGGCCGATTGTGGCACCGCACAGGCGTAATACTCAGCAGGAGATAACAATGAAAATTTTCCAACGGTACAACCCACTTCAGGTGGCGAAGTACGTAAAGATCCTGTTCCGTGGACGGTTATACATCAAGGACGTTGGCGCTTTTGAATTCGATAAGGGTAAGATTCTTATCCCAAAAGTGAAGGATAAGCTGCATTTGTCAGTGATGTCCGAAGTTAACCGTCAGGTTATGCGTCTGCAAACAGAGATGGCTTAACCAAAGTGCTATGCAGTAAAAAGT  >  NC_000913/4439335‑4439817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑