Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,340,953	C→T	G226R (GGG→AGG)	melR ←	DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,953	0	C	T	100.0%	219.5 / NA	73	G226R (GGG→AGG)	melR	DNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/35);  total (38/35)

TGATATCGTCTTTAGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGT  >  NC_000913/4340709‑4341192                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                tgataTCGTCTTTAGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGc                                                                                                                                                                                                                                           >  1:563135/1‑251 (MQ=255)  gataTCGTCTTTAGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTCCTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGct                                                                                                                                                                                                                                          >  1:464876/1‑250 (MQ=255)         tCTTTAGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAATTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTAATTGAACGAAAGCCTGCCGTCACGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAAAGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGCATATCCTCATTGCATAGTTgg                                                                                                                                                                                                                                   >  2:580304/1‑251 (MQ=255)               gCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTaa                                                                                                                                                                                                                             <  2:464876/251‑1 (MQ=255)                ccGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTa                                                                                                                                                                                                                              >  2:348954/1‑249 (MQ=255)                     aaaCGTCTGGCGGCGCTGTTGGCTACGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTgc                                                                                                                                                                                                                       >  2:98236/1‑250 (MQ=255)                             ggcggcGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCa                                                                                                                                                                                                               <  1:326141/251‑1 (MQ=255)                                  cgcTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTTCTCAGCcac                                                                                                                                                                                                          <  2:323809/251‑1 (MQ=255)                                    cTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACCTATTTGCCGAACGTGCTGTAAAAACGCCTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACAt                                                                                                                                                                                                        >  2:259233/1‑251 (MQ=255)                                    cTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGttt                                                                                                                                                                                                                         >  1:169007/1‑234 (MQ=255)                                    cTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGttt                                                                                                                                                                                                                         <  2:169007/234‑1 (MQ=255)                                     tGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATc                                                                                                                                                                                                       >  2:258554/1‑251 (MQ=255)                                             aaGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGAt                                                                                                                                                                                                 <  1:576235/249‑1 (MQ=255)                                             aaGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATAATTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTGGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACACGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGGGCGCAGCAACATCGTTGATgg                                                                                                                                                                                               >  2:487270/1‑251 (MQ=255)                                              aGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCTTAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGt                                                                                                                                                                                              >  2:181864/1‑251 (MQ=255)                                                   gCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTAGCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAgc                                                                                                                                                                                          <  1:180995/250‑1 (MQ=255)                                                   gCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCGGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAgcg                                                                                                                                                                                         <  1:397636/251‑1 (MQ=255)                                                      ggaTTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCt                                                                                                                                                                                      <  2:474544/249‑1 (MQ=255)                                                      gTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTGGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGGTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCt                                                                                                                                                                                      <  2:305674/251‑1 (MQ=255)                                                        aTTGTTGCGGCGACTTGCCGACATATTTGCCGAACGTGCCGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGa                                                                                                                                                                                    <  2:434552/251‑1 (MQ=255)                                                               cggcgACATGCCGACATATTTGCCTAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCGGCCGTCATGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTGTCATCGTCTATTGCATGACCCGCTGGAATATGCTCATTGCATAGTTGGCGTTAAGTTTGACGGGCTCAGCCATAGCGTAGATTGTCAGCGCCTGATCATAGt                                                                                                                                                                             <  2:562243/251‑1 (MQ=255)                                                                ggcgACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGTTCAgcg                                                                                                                                                                                         >  2:164493/1‑238 (MQ=255)                                                                ggcgACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAgcg                                                                                                                                                                                         <  1:164493/238‑1 (MQ=255)                                                                      aTGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTGGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGc                                                                                                                                                                      <  1:555946/251‑1 (MQ=255)                                                                        gCCGACATATTTGCCGAACGTGCTGTAAACACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGCGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCa                                                                                                                                                                     >  2:420907/1‑250 (MQ=255)                                                                           gACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGATCGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTACCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATaa                                                                                                                                                                 <  1:534271/251‑1 (MQ=255)                                                                                 tttGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGccc                                                                                                                                                            >  1:229944/1‑250 (MQ=255)                                                                                  ttGCTGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGccc                                                                                                                                                            >  1:321436/1‑249 (MQ=255)                                                                                     ccGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCAt                                                                                                                                                       <  1:292339/251‑1 (MQ=255)                                                                                         aCGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTgg                                                                                                                                                   <  2:24117/251‑1 (MQ=255)                                                                                          cGTGCTGTAAAAACGACTACTTGAACGAAAGCGTGCCGTCAGGGCAATATCGAGACTAATTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGAGCATCGCGGTAATGTACTGTTTAATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAATTGGCGTTACGTTTGACGTGATCAGACACATCGTTGATGGACAGCGCCTGCTCATAGTTTTCGGCAATAAAGCCCAGCATCTGGc                                                                                                                                                  >  1:535965/1‑251 (MQ=255)                                                                                            ggCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTa                                                                                                                                                <  2:281847/250‑1 (MQ=255)                                                                                                gTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACa                                                                                                                                             <  2:522555/250‑1 (MQ=255)                                                                                                        gACTACATGAACGCACGCCTGCCGACAGGCCAGTAGCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGACTGAGGCGCATGGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGCTGGCGTTAAGTTTGACGCGCTCAGCCACATCGTTGATGGTCATCGCCTGATCATAGTTCTCGGCAATAAAGCCCAGCATCTGGCTACCATAAAATTgc                                                                                                                                    >  2:3332/1‑251 (MQ=255)                                                                                                                gAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGgc                                                                                                                             <  1:122926/250‑1 (MQ=255)                                                                                                                      aaGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGAc                                                                                                                      <  2:127140/251‑1 (MQ=255)                                                                                                                            gCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTg                                                                                                                  <  1:446042/249‑1 (MQ=255)                                                                                                                                 cAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGTACGTGGTTGATGCGCAGCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGTCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGTCGCTGTTTTtgt                                                                                                           <  1:110857/251‑1 (MQ=255)                                                                                                                                   gggCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTtgtgt                                                                                                         <  2:258316/251‑1 (MQ=255)                                                                                                                                              agaATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGt                                                                                              <  1:388087/251‑1 (MQ=255)                                                                                                                                               gaATTCTTTTAACGGTATCGCTCAGTAACGCGCGTACGTGGTTGATGCGCATCGCGGTAATGTACTGTTGCATCGTCAATTGCATGACCCGCTGGAAGATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCTCAGCATCTGGCTAACATAAAATTGCGCATGTCGCAAGACGCTGTTTTTGTGTGTGCGCGAGGtt                                                                                             <  2:427747/251‑1 (MQ=255)                                                                                                                                                       ttATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGAc                                                                                     >  1:629171/1‑251 (MQ=255)                                                                                                                                                            ggTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGAAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGaa                                                                                <  1:258554/251‑1 (MQ=255)                                                                                                                                                             gTATCGCGCAGTAACGAGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTACATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGACACATCGTTGATGGTCAGCGCCTGATCCTAGTTTTCGGCAATAAATCCCAGCATCTGGCTAACATAAAATTGCGCATGGAGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAAt                                                                               >  2:240855/1‑251 (MQ=255)                                                                                                                                                                 cGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGt                                                                           >  1:192471/1‑251 (MQ=255)                                                                                                                                                                  gctccgcaaagcgcgAACATGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACCTCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAACATTGCGCATGGCGCGCGACGCTGTTTTTGTGTGTGCGCGCGGCTTTATTGACCAGAATCGGtt                                                                          >  2:87359/11‑251 (MQ=255)                                                                                                                                                                            gcgcgAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCccagccagg                                                                >  2:232831/1‑250 (MQ=255)                                                                                                                                                                              gcgAACGGGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGGCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCACGAGGTTTTATTGACCAGAATCGGTTCcaagcccggg                                                               >  2:1761/1‑246 (MQ=255)                                                                                                                                                                                gAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGAGAGGTTTTATTGACAAGAATCGGTTCCCATCAAGAGAgg                                                            >  2:466105/1‑251 (MQ=255)                                                                                                                                                                                    gTGGTTGCTGCGCATCGAGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTa                                                         >  1:169523/1‑250 (MQ=255)                                                                                                                                                                                      ggTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAAt                                                      >  1:431360/1‑251 (MQ=255)                                                                                                                                                                                                 aTCGCGGTAATGTACTGTTTCATCGTCAATTGCCTGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCt                                                                                                                   >  2:448820/1‑179 (MQ=255)                                                                                                                                                                                                 aTCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGgc                                                                                                                             >  2:448152/1‑169 (MQ=255)                                                                                                                                                                                                 aTCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGgc                                                                                                                             <  1:448152/169‑1 (MQ=255)                                                                                                                                                                                                 aTCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCt                                                                                                                   <  1:448820/179‑1 (MQ=255)                                                                                                                                                                                                    gcgGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATc                                          >  1:589670/1‑249 (MQ=255)                                                                                                                                                                                                      ggTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCCGCCagaga                                                              >  2:167471/1‑227 (MQ=255)                                                                                                                                                                                                      ggTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCagaga                                                              <  1:167471/227‑1 (MQ=255)                                                                                                                                                                                                       gTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTGTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGcc                                     <  1:293269/251‑1 (MQ=255)                                                                                                                                                                                                         aaTGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTAGTGACCAGAATCGGTTCCCAGCCAGAGATGCTAAATCGCTTGa                                               <  1:112563/239‑1 (MQ=255)                                                                                                                                                                                                         aaTGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGAGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTgc                                               >  2:112563/1‑238 (MQ=255)                                                                                                                                                                                                         aaTGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCAGTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCTCATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTCTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCaa                                   <  1:284895/251‑1 (MQ=255)                                                                                                                                                                                                                tGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTtcatc                            >  2:462796/1‑251 (MQ=255)                                                                                                                                                                                                                   ttaattgtaatttgaataaccagCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATTAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGGCCAGAATCGGTGCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAAt                         >  2:287419/23‑251 (MQ=255)                                                                                                                                                                                                                   ttCATCGTCATTTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTGCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGGTAAATCGCTTGAGCATCATGCCAATTTCATCaa                          <  1:185998/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTTGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGACTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGACAATTTCATCAATGGCg                     >  1:421897/1‑251 (MQ=255)                                                                                                                                                                                                                           cAATTGCATGACCCGCTGGAATCTCCTCATTGCATAGTTGGCGTTAAGTTTGACGCGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATACAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCt                 >  2:55825/1‑251 (MQ=255)                                                                                                                                                                                                                            aaTTGCGTGACTCGCAGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTCTTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTg                >  2:55794/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCt     >  1:79010/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccGCTGGAATATCCTCATTGCAGAGTTGGCGTTAAGTTTGACGTGCTCAGCCACAACGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTTGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGATCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCATGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCt     <  2:323037/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccGCTGGAATATAATCATTGCCTAGTTGGCGTTCAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCt     >  2:49854/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGCTGGAATATCCTCAGTGCATAGTGGGCGTGAAGTTTGACGTGCGCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAAGCGGTTCCCAGCCAGAGAGGCTAAAGCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTc    <  2:88193/251‑1 (MQ=255)                                                                                                                                                                                                                                          cTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGt  >  2:181133/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TGATATCGTCTTTAGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGT  >  NC_000913/4340709‑4341192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑